Dr. Alfio Comis

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Adelaide University
MAbSA Technologies
Managing ScientistAppointed: 2005
Professional Headshot of Alfio  Comis

Mailing Address

MAbSA Technologies
Molecular and Biomedical Science
University of Adelaide
North Terrace Campus
Adelaide, South Australia 5005
Australia

Contact Information

Phone: +61 8 8303 3524
Mobile: 0434072524
alfio.comis@adelaide.edu.au
http://www.adelaide.edu.au/mbs/mabsa/

Qualifications

Ph.D., University of Sydney, Biochemistry, 1988.
Hons, University of New South Wales, Biochemistry, 1981.
B.Sc., University of New South Wales, Science, 1980.

Expertise and Research Interests

My expertise is in hybridoma technology. I have had 18 years experience in producing specific monoclonal antibody-secreting hybridoma. I have also set up 7 separate monoclonal antibody facilities at different Universities and private companies in Australia and the USA.

Although most of my past experience has been with mouse monoclonal antibodies, I am currently also working with rat and human monoclonals.

I am currently the Managing Scientist of MAbSA Technologies, a joint venture between the University of Adelaide and the Institute of Medical and Veterinary Science in Adelaide, Australia. The company specialises in custom monoclonal antibody service where we produce new hybridoma cell lines, as well as producing affinity-purified monoclonal antibodies from existing cell lines. Our cells are grown in serum-free media to help the scientist in their specific assays.

Other Expertise

Plant Proteomics
Two dimensional electrophoresis
Peptide synthesis
Diagnostic kits
Receptor-ligand binding studies
Combinatorial chemistry
Animal toxin isolation and characterization

Future Research

Future research will focus on improving hybridoma technology methods to include chicken and rabbit monoclonal antibodies. Also new ways to immunise animals with specific cells genetically modified to express difficult membrane proteins of interest on their own membrane system.

Industrial Relevance

For three years, I was in charge of the research conducted by Deakin Research, a small Australian Biotechnology Company working on the development of synthetic vaccines and diagnostic kits. This work resulted in a patent to produce oral, enzymatically-stable, retro-inverso immunogens.

Currently, I am running MAbSA Technology, focussing on the production of hybridoma cell lines and their products, as well as their use and commercialization in diagnostic kits and research tools.

Keywords

COS Keywords:

Agricultural Biotechnology, Electrophoresis, Genomics, Immunoprecipitation, Molecular Biology, Monoclonal Antibodies, Peptides, Plant Genetics, Proteomics, Recombinant DNA, Signal Transduction.

Additional Terms:

2-D Gel Electrophoresis, Chicken Antibodies, Diagnostic Kits, Hybridoma Technology, Immunolocalization, Membrane Proteins, Membrane Receptors, Monoclonal Antibodies, Phospho-proteome, Post-translation Modifications in Proteins, Protein Structure, Proteomics, Retro-inverso Peptides, Signal Transduction, Stress Physiology, Transgenic Crops, Western Blots.

Languages

(Reading, Writing, Speaking)

English: (Fluent, Fluent, Fluent)
Italian: (Fluent, Fluent, Fluent)

Memberships

Australian Society for Biochemistry and Molecular Biology
Centre for Biostructural and Biomolecular Research
Crop Science Society of America
Sydney Protein Group

Previous Positions

2003-2005, Research Fellow, Adelaide University, Australian Centre for Plant Functional Genomics
2001-2003, Associate Professor, Cornell University, Agriculture and Life Sciences, Crop & Soils Sciences, Biochemistry
1997-2000, Tenured Faculty Member, University of Western Sydney, Science and Technology, Centre for Biostructural and Biomolecular Research
1995-1997, NH&MRC Senior Research Officer, University of New South Wales, Faculty of Medicine, Physiology and Pharmacology
1990-1995, Senior Research Scientist, Deakin Research Ltd.
1988-1990, NH & MRC Postdoctoral Research Fellow, Deakin University, Biological Sciences
1986-1988, NH & MRC Postdoctoral Research Fellow, Macquarie University, Environmental and Life Sciences, Chemistry

Patents

Vascular plants expressing Na+ pump ATPase, Patent Number: PCT- AU2005/001553, 2005, Industry, United States of America.
Retro-, inverso-, and retro-inverso peptide analogs and their use as antigens., Patent Number: 6261569 B1, 2001, Industry-owned, United States of America.

Funding Received

  • Australian Research Council: The effect of proline on the three-dimensional structure of retro-inverso peptides., AU$14,000, Jan 1, 2000 to Dec 31, 2000.
  • Australian Research Council: Symbiotic implications of type III protein secretions in rhizobia bacteria., AU$13,453, Jan 1, 2000 to Dec 31, 2000.
  • Australian Research Council: Monoclonal antibodies to tachykinin receptors., AU$11,735, Jan 1, 1999 to Dec 31, 1999.
  • Australian Academic Development Fund: Integrating Web-based Chemistry and Biochemistry teaching with the Hawkewsbury Shell, AU$19,970, Jan 1, 1998 to Dec 31, 2000.
  • Australian APA Industry Scholarship: Synthesis of metabolically stable 'retro-inverso' analogues of neurokinin A and assessment of their pharmacological activity in human bladder., AU$105,000, Jan 1, 1997 to Dec 31, 2000.
  • Australian Research Council: Antisense peptides. A novel approach to receptor structure/function., AU$10,997, Jan 1, 1996 to Dec 31, 1996.
  • United States Department of Agriculture (USDA): Impact of transgenic corn on the soil microbial food web, US$30,000, Feb 2002 to Nov 2004.
  • ARC - Linkage Infrastructure (Application submitted): Adelaide Mass spectrometer Analysis Network, AU$657,054, 2006 to .
  • BioInnovation SA: MAbSA Technology, AU$160,000, 2005 to 2007.

Publications

  • Jacobs A.K., Lunde C., Juttner J., Comis A., Drew D., Plett D., Tester M. (2006) Functional analysis of a sodium transport gene expressed in model and crop species, Plant & Animal Genomes XIV Conference, P804 pages
  • Malone J., Comis A., Oldach K. (2006) Analysis of signal pathway protein-protein interactions during biotic and abiotic stress, XV Congress of the FESPB, Federation of European Societes of plant Biology, In Press
  • Malone J, Shirley N., Comis A., Langridge P., Oldach K. (2005) Analysis of signal pathway protein-protein interactions during biotic and abiotic stress, ComBio 2005
  • Dolman F., Juttner J., Baumann U., Comis A., Shirley N., Langridge P. (2005) Plant cytosolic Thioredoxin expression and protein-protein interactions in Hordeum vulgare reproductive tissues, ComBio 2005
  • Comis A., Malone J., Oldach K. (2005) 14-3-3 affinity purification of phosphoproteins in barley during biotic and abiotic stress, 30th Annual Lorne Conference on Protein Structure and Function
  • Oldach K., Comis A. (2005) Screening for differentially-expressed proteins in the host-pathogen interaction between barley and the fungus Rhinchosporium secalis, 10th Lorne Proteomics Symposium
  • Harman G.E., Comis A., Chen J. (2005) Proteins related to the biocontrol of Pythium damping-off in maize with Trichoderma harzianum Rifai, J. Integrative Plant Biol. (Acta Botanica Sinica), 47 (8), 988-997
  • Juttner J., Comis A., Dolman F., Langridge P., Baumann U. (2004) Molecular characterization and stress-responsive expression of the thioredoxin h family in the grasses, 1st Annual ACPFG Research Symposium: Genomics in the Barossa
  • Chen J., Harman G.E., Comis A. (2004) Proteomics related to the biocontrol of Pythium damping off in maize with trichoderma harzianum, J. ZheJiang University (Agriculture & Life Sciences, 30 (4), 441
  • Chen J., Comis A., Harman G.E. (2004) Effects of Trichoderma harzianum and Pythium ultimum on the function of maize plant and its proteome, XVth International Plant Protection Congress
  • Harman G.E., Petzoldt R., Comis A., Chen J., Interactions between Trichoderma harzianum strain T22 and maize inbred line Mo17 and effects of this interaction on diseases caused by Pythium ultimum and Colletotrichum graminicola, Phytopathology, 94(2), 147-153, February 2004
  • Emmanuel C., Foo E., Medbury H.J., Matthews J., Comis A., Zoellner H., Synergistic induction of apoptosis in human endothelial cells by tumour necrosis factor-alpha and transforming growth factor-beta, Cytokine, 18(5), 237-241, 7 Jun 2002
  • Warner F.J., Mack P., Comis A., Miller R.C., Burcher E., Structure-activity relationships of neurokinin A (4-10) at the human tachykinin NK2 receptor: the role of natural residues and their chirality, Biochemical Pharmacology, 61(1), 55-60, 01 2001
  • Liu L., Shang F., Perry M.A., Comis A.,Burcher E., Bufokinin: Immunoreactivity, receptor localization and actions in toad intestine and mesenteric circulation, Peptides, 21(9), 1345-1354, 09 2000
  • Liu L., Shang F., Comis A., Burcher E., Bufokinin: actions and distribution in the toad cardiovascular system, Clinical and Experimental Pharmacology and Physiology, 27(11), 911-916, 11 2000
  • Chapman T., Medbury H., Comis A., Zoellner H. (1999) Polar nuclear labeling of DNA by bromodeoxyuridine, Aust. Soc. Med. Res. 38th National Meeting
  • Matuszek M.A., Comis A., Burcher E., Binding and functional potency of neurokinin A analogs in the rat fundus. A sytructure-activity study, Pharmacology, 58(5), 227-235, 05 1999
  • McGlasson WB, Holford P, Comis A, Abdi N, Fruit maturity, new and practical methods for objective measurement, Annual Technical Update for the Summer Fruit Industry of Western Australia, 1999
  • McGlasson B., Holford P., Comis A., Abdi N., A new reliable maturity index for stone fruit, Australian Fresh Stone Fruit Quarterly, 1, 15-16, 1999
  • Warner F.J., Comis A., Miller R.C., Burcher E. (07 1999) Characterization of the [125I]-neurokinin A binding site in the circular muscle of human colon, British Journal of Pharmacology, 127 (5), 1105-1110
  • Comis A., Burcher E. (03 1999) Structure-activity studies at the rat tachykinin NK2 receptor: effect of substitution at position 5 of neurokinin A, Journal of Peptide Research, 53 (3), 337-342
  • Burcher E., Markus I., Shang F., Comis A. (1998) Actions of tachykinins and neurotensin and tachykinin-like immunoreactivity in human colon, Annual Meeting American Gastroenterology Society
  • Burcher E., Markus I., Shang F., Comis A., Lubowski D.Z., Actions of tachykinins and neurotensin and tachykinin-like immunoreactivity in human colon, Gastroenterology, 114(4), G3010, 15 Apr 1998
  • Burcher E., Shang F., Markus I., Lubowski D.Z., Comis A. (1998) Neurokinin A and substance P-like immunoreactivity in muscle, mucosa and ganglia of human colon, Australian Neuroscience Society
  • Matuszek M.A., Comis A., Burcher A. (1997) Smooth muscle, neurons and interstitial cells of guinea-pig ileum: are there tachykinin NK-1 receptor subtypes?, Tachykinin in Health and Disease
  • Pearson J.A., Barnett D., Comis A., Connor M., Harrison B.M., Lloyd D.R., Nicholson G.M., Nicholson P., Retson K.V., Spence I., Tyler M.I., Wilson H.I., Howden M.E.H., Protein toxins and their use in cell biology, In: Protein toxins and their use in cell biology. Editors: Rappuoli R., Montecucco C., 228-231, 1997
  • Matuszek M.A., Comis A., Burcher E. (1997) Muscle, neuronal and cellular regions of guinea-pig ileum: are there tachykinin receptor subtypes?, Proc. Aust. Soc. Clin. Exp. Pharmacol. Toxicol
  • Comis A., Burcher E. (1996) Binding to the NK2 receptor by an antibody to an antisense substance P, Second Australian Peptide Meeting
  • Comis A., Tyler M.I., Vonarx E.J., Atkinson R.K., Mylecharane E.J., Spence I., Howden M.E.H., Synthetic approaches to spider venom peptide toxins, Abstracts of papers of the American Chemical Society, 209, 28, 2 Apr 1995
  • Comis A., tyler M.I., Vonarx E.J., Atkinson R.K., Mylecharane E.J., Spence I, Wilson H.I., Howden M.E.H. (1995) Synthetic approaches to spider venom peptide toxins, Abstracts of Papers of the American Chemical Society
  • Collins S.P., Comis A., Tyler M.I., Marshall M., Howden M.E.H., Evidence for a high molecular weight pre-robustoxin molecule in the venom of the male Sydney funnel-web spider (Atrax robustus), Comparative Biochemistry and Physiology, 110C, 89-93, 1995
  • Collins S.P., Comis A., Marshall M., Hartwick R.F., Howden M.E.H., Monoclonal antibodies neutralizing the haemolytic activity of box jellyfish (Chironex fleckerii) tentacle extracts, Comparative Biochemistry and Physiology, 106B, 67-70, 1993
  • Mylecharane E.J., Spence I., Comis A., Tyler M., Howden M.E.H., Immunization with a synthetic robustoxin derivative lacking disulphide bridges protects anaesthetised monkeys against potentially lethal challenge with a male funnel-web spider (Atrax robustus) venom, Recent advances in Toxicology Research; eds P. Gopalakrishnakone and C.K. Tan, 2, 163-168, 1992
  • Sheumack D.D., Comis A., Claassens R., Milecharane E.J., Spence I., Howden M.E.H., An endogenous antitoxin to the lethal venom of the funnel web spider, Atrax robustus, in rabbit sera, Comparative Biochemistry and Physiology, 99C, 157-161, 1991
  • Sheumack D.D., Phillips C.A., Mylecharane E.J., Spence I., Claassens R., Brown M.R., Comis A., Howden M.E.H., Protection of monkeys against the lethal effects of male funnel-web spider (Atrax robustus) venom by immunization with a toxoid, Toxicon, 29, 603-611, 1991
  • Comis A., Hartwick R.F., Howden M.E.H., Stabilization of lethal and haemolytic activities of box jellyfish (Chirones fleckerii) venom, Toxicon, 27, 439-447, 1989
  • Fisher P.M., Comis A., Howden M.E.H. (1989) Direct immunisation with synthetic peptidyl-polyamide resin. Comparison with antibody production from free peptide and conjugates with carrier protein, Journal of Immunological Methods, 119, 118-123
  • Holland R.A.B., Rimes A.F., Comis A., Tyndale-Biscoe C.H., Oxygen carriage and carbonic anhydrase activity in the blood of a marsupial, the tammar wallaby (Macropus eugenii) during early development, Respiratory Physiology, 73, 69-86, 1988
  • Comis A., Tyler M.I., Mylecharane E.J. Spence I., Sheumack D.D., Howden M.E.H., Immunochemistry of funnel-web spider venoms, Proceedings of the Sydney Allergen Group, 6, 37-48, 1988
  • Holland R.A.B., Comis A., Carbonic anhydrase activity in the blood of adult sheep and lambs, Comparative Biochemistry and Physiology, 86, 673-678, 1987
  • Comis A., Easterbrook-Smith S.B., Binding of complement component C1q by spectrin, Biochimica et Biophysica Acta, 870, 426-431, 1986
  • Comis A., Easterbrook-Smith S.B., Inhibition of serum complement haemolytic activity by lipid vesicles containing phosphatidylserine, FEBS Letters, 197, 321-326, 1986
  • Comis A., Easterbrook-Smith S.B., Binding of complement component C1q by rat adipocyte membranes, Molecular Immunology, 22, 857-861, 1985
  • Comis A., Easterbrook-Smith S.B., C1q binding to mitochondria: a possible artefact?, FEBS Letters, 185, 105-108, 1985
  • Comis A., Easterbrook-Smith S.B., Evidence for arginine residues in the immunoglobulin-binding site of human C1q, Biochimica et Biophysica Acta, 842, 45-51, 1985
  • Comis A., Easterbrook-Smith S.B., Chemical modification of arginyl residues in human C1q, Proceedings of the Australian Biochemical Society, 16, 19, 1984
  • Comis A., Easterbrook-Smith S.B. (1984) Chemical modification of arginyl residues in human C1q, Proc. Australian Biochem. Soc.
  • Comis A., Easterbrook-Smith S.B., Modification of rabbit IgG with fluorescein isothiocyanate, Proceedings of the Australian Biochemical Society, 15, 25, 1983
  • Comis A., Easterbrook-Smith S.B. (1983) Modification of rabbit IgG with fluorescein isothiocyanate, Proc. Australian Biochem. Soc.
  • Malone J., Analysis of signal pathway protein-protein interactions during biotic and abiotic stress, XV Congress of the FESPB, Federation of European Societes of plant Biology, In Press

Profile Details

Last Updated: 8/15/2007

COS Expertise ID #908516
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