Dr. David Fredricks

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Fred Hutchinson Cancer Research Center
Clinical Research Division
Vaccine and Infectious Diseases Institute
Program in Infectious Diseases
Associate MemberAppointed: 2007
University of Washington
School of Medicine
Medicine
Allergy and Infectious Diseases
Associate ProfessorAppointed: 2007
Professional Headshot of David Fredricks

Mailing Address

Fred Hutchinson Cancer Research Center
P.O. Box 19024
Vaccine and Infectious Disease Institute
D3-100
Seattle, Washington 98109-1024
United States

Contact Information

dfredric@fhcrc.org

Qualifications

M.D., Case Western Reserve University, Medicine, 1990.

Expertise and Research Interests

The Fredicks laboratory develops and applies molecular diagnostic tests for the detection and identification of microbial pathogens, including those pathogens that are difficult to culture in the laboratory. We employ the polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) to detect and localize bacteria and fungi in human tissues. These molecular approaches are applied to improve the detection of well known pathogens, and to investigate the role of novel or uncultivated microbes in unexplained human diseases (pathogen discovery). The laboratory has developed a suite of PCR assays for the detection of fungal pathogens in immunocompromised patients.

We also study the constituents of the human indigenous microbiota, using cultivation-independent approaches to characterize microbes present on human mucosal and epithelial surfaces. The microbiology of the human vagina is an area of research focus, and we are particularly interested in the role of fastidious bacteria in the syndrome bacterial vaginosis. We also study the role of fastidious vaginal bacteria in other adverse health conditions in women.

Other Expertise

Previous research has been performed on the cultivation-resistant pathogens Tropheryma whipplei (bacterial cause of Whipple Disease) and Rhinosporidium seeberi (protistan parasite that causes nasal and ocular tumors in rhinosporidiosis).

Keywords

COS Keywords:

Infectious Diseases or Agents, Microbiology, Sexually Transmitted Diseases.

Additional Terms:

Bacterial Infections, Bacterial Vaginosis, Female Genital Disorders, Fungal Diseases, Infectious Diseases or Agents, Microbiology, Microbiome, Sexually Transmitted Diseases.

Memberships

American Association for the Advancement of Science
American Society for Microbiology
Infectious Diseases Society of America

Honors and Awards

Physician Scientist Award, National Institutes of Health, Stanford University
Program Commitee Choice Award, Infectious Diseases Society of America, Best abstract at 2004 annual meeting, Boston.
James McDougall Faculty Mentor Award, Fred Hutchinson Cancer Research Center

Previous Positions

2001-2007, Assistant Member, Fred Hutchinson Cancer Research Center, Clinical Research Division, Program in Infectious Diseases
2001-2007, Assistant Professor, University of Washington, School of Medicine, Medicine, Allergy and Infectious Diseases
1998-2001, Research Associate, Stanford University, School of Medicine, Medicine, Infectious Diseases

Funding Received

  • National Institutes of Health (NIH): R01, The role of uncultivated bacteria in bacterial vaginosis, 2006 to .
  • National Institutes of Health (NIH): R01, Quantitative PCR for the detection of fungal infections, 2004 to .

Publications

  • Nelson D, Hanlon A, Hassan S, Britto J, Geifman-Holtzman O, Haggerty C, Fredricks DN (2008) Preterm labor and bacterial vaginosis-associated bacteria among urban women, Journal of Perinatal Medicine
  • Oakley BB, Fiedler TL, Marrazzo JM, Fredricks DN (2008) The Diversity of Human Vaginal Bacterial Communities and their Association with Clinically-Defined Bacterial Vaginosis, Applied and Environmental Microbiology, 74, 4898-909
  • Khot PD, Ko DL, Hackman RC, Fredricks DN (2008) Development and Optimization of quantitative PCR for the diagnosis of invasive aspergillosis using bronchoalveolar lavage fluid, BioMed Centra Infectious Diseases, 8, 73
  • Marrazzo JM, Thomas KK, Fiedler TL, Ringwood K, Fredricks DN (2007) Relationship of Specific Vaginal Bacteria and Bacterial Vaginosis Treatment Failure in Women Who Have Sex with Women, Annals of Internal Medicine, 149, 20-28
  • Fredricks DN, Fiedler TL, Thomas KK, Oakley BB, Marrazzo JM (2007) Targeted PCR for Detection of Vaginal Bacteria Associated with Bacterial Vaginosis, Journal of Clinical Microbiology, 45 (10), 3270-3276
  • Fredricks DN, Fiedler TL, Marrazzo JM (Nov 2005) Molecular identification of bacteria associated with bacterial vaginosis., 353 (18), 1899-911 Abstract
  • Fredricks DN, Smith C, Meier A (Oct 2005) Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR., 43 (10), 5122-8 Abstract
  • Fredricks DN, Schubert MM, Myerson D (Jul 2005) Molecular identification of an invasive gingival bacterial community., 41 (1), e1-4 Abstract
  • Musher B, Fredricks D, Leisenring W, Balajee SA, Smith C, Marr KA (Dec 2004) Aspergillus galactomannan enzyme immunoassay and quantitative PCR for diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid., 42 (12), 5517-22 Abstract
  • Imhof A, Balajee SA, Fredricks DN, Englund JA, Marr KA (Sep 2004) Breakthrough fungal infections in stem cell transplant recipients receiving voriconazole., 39 (5), 743-6 Abstract
  • Maiwald M, von Herbay A, Fredricks DN, Ouverney CC, Kosek JC, Relman DA (Sep 2003) Cultivation of Tropheryma whipplei from cerebrospinal fluid., 188 (6), 801-8 Abstract
  • Fredricks DN, Martin TM, Edwards AO, Rosenbaum JT, Relman DA, Human herpesvirus 8 and sarcoidosis, Clinical Infectious Diseases, 34(4), 559-60, February 2002
  • Fredricks DN (Dec 2001) Microbial ecology of human skin in health and disease., 6 (3), 167-9 Abstract
  • Fredricks DN, Relman DA, Localization of Tropheryma whippelii rRNA in tissues from patients with Whipple's disease, Journal of Infectious Diseases, 183(8), 1229-37, 2001 Abstract
  • Maiwald M, von Herbay A, Persing DH, Mitchell PP, Abdelmalek MF, Thorvilson JN, Fredricks DN, Relman DA, Tropheryma whippelii DNA is rare in the intestinal mucosa of patients without other evidence of Whipple disease, Annals of Internal Medicine, 134(2), 115-9, 2001 Abstract
  • Fredricks DN, Jolley JA, Lepp PW, Kosek JC, Relman DA, Rhinosporidium seeberi: a human pathogen from a novel group of aquatic protistan parasites, Emerging Infectious Diseases, 6(3), 273-82, 2000 Abstract
  • Relman DA, Fredricks DN, Yoder KE, Mirowski G, Berger T, Koehler JE, Absence of Kaposi's sarcoma-associated herpesvirus DNA in bacillary angiomatosis-peliosis lesions, Journal of Infectious Diseases, 180(4), 1386-9, October 1999 Abstract
  • Fredricks DN, Relman DA, Application of polymerase chain reaction to the diagnosis of infectious diseases, Clinical Infectious Diseases, 29(3), 475-86, September 1999 Abstract
  • Fredricks DN, Relman DA, Paraffin removal from tissue sections for digestion and PCR analysis, Biotechniques, 26(2), 198-200, February 1999 Abstract
  • Fredricks DN, Relman DA, Improved amplification of microbial DNA from blood cultures by removal of the PCR inhibitor sodium polyanetholesulfonate, Journal of Clinical Microbiology, 36(10), 2810-6, October 1998 Abstract
  • Petrides PE, Muller-Hocker J, Fredricks DN, Relman DA, PCR analysis of T. whippelii DNA in a case of Whipple's disease: effect of antibiotics and correlation with histology, American Journal of Gastroenterology, 93(9), 1579-82, September 1998 Abstract
  • Fredricks DN, Relman DA, Infectious agents and the etiology of chronic idiopathic diseases, Current Clinical Topics in Infectious Diseases, 18, 180-200, 1998 Abstract
  • Fredricks DN, Rojanasthien N, Jacobson MA, AIDS-related disseminated histoplasmosis in San Francisco, California, Western Journal of Medicine, 167(5), 315-21, November 1997 Abstract
  • Fredricks DN, Relman DA, Cultivation of Whipple bacillus: the irony and the ecstasy, Lancet, 350(9087), 1262-3, 1997 Abstract
  • Lynch T, Odel J, Fredericks DN, Louis ED, Forman S, Rotterdam H, Fahn S, Relman DA, Polymerase chain reaction-based detection of Tropheryma whippelii in central nervous system Whipple's disease, Annals of Neurology, 42(1), 120-4, July 1997 Abstract
  • Ramzan NN, Loftus E Jr, Burgart LJ, Rooney M, Batts KP, Wiesner RH, Fredricks DN, Relman DA, Persing DH, Diagnosis and monitoring of Whipple disease by polymerase chain reaction, Annals of Internal Medicine, 126(7), 520-7, 1997 Abstract
  • Fredricks DN, Remington JS, Tularemia presenting as community-acquired pneumonia. Implications in the era of managed care, Archives of Internal Medicine, 156(18), 2137-40, 1996 Abstract
  • Marth T, Fredericks D, Strober W, Relman DA, Limited role for PCR-based diagnosis of Whipple's disease from peripheral blood mononuclear cells, Lancet, 348(9019), 66-7, 1996 Abstract
  • Banuelos AF, Williams PL, Johnson RH, Bibi S, Fredricks DN, Gilroy SA, Bhatti SU, Aguet J, Stevens DA, Central nervous system abscesses due to Coccidioides species, Clinical Infectious Diseases, 22(2), 240-50, February 1996 Abstract
  • Wagner JA, Keer H, Fredricks DN, Disseminated coccidioidomycosis diagnosed by culture of a central venous catheter tip, Clinical Infectious Diseases, 22(1), 180-1, January 1996 Abstract
  • Fredericks DN, Relman DA, Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates, Clinical Microbiology Reviews, 9(1), 18-33, January 1996 Abstract
  • Kirn DH, Fredericks D, McCutchan JA, Stites D, Shuman M, Serum ferritin levels correlate with disease activity in patients with AIDS and disseminated histoplasmosis, Clinical Infectious Diseases, 21(4), 1048-9, October 1995 Abstract

Profile Details

Last Verified: 2/28/2009

COS Expertise ID #345988
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