George W. Smith

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Michigan State University
College of Natural Science
Physiology
Adjunct Associate ProfessorAppointed: 2003
Michigan State University
College of Agriculture & Natural Resources
Animal Science
Associate ProfessorAppointed: 2003

Mailing Address

Department of Animal Science
Michigan State University
1230D Anthony Hall
East Lansing, Michigan 48824-1225
United States

Contact Information

Phone: (517) 432-5401
Fax: (517) 353-1699
Smithge7@msu.edu

Qualifications

Ph.D., University of Missouri-Columbia, Animal Science, 1996.
M.S., University of Missouri-Columbia, Animal Science, 1992.
B.S., University of Idaho, Animal Science, 1989.

Expertise and Research Interests

Research efforts in my laboratory over the past years have focused on investigation of fundamental mechanisms that regulate ovarian function in farm animals and the physiological mechanisms involved in regulation of the stress response. We have obtained new insight into the hormonal regulation and regulatory role of members of the matrix metalloproteinase gene family in control of follicle rupture and the hormonal regulation and physiological role of various components of the corticotropin releasing factor (CRF) system in promoting the stress response. Recent research efforts in my laboratory utilize functional genomics approaches to identify specific components of the oocyte transcriptome [catalog of genes expressed in the female germ cell] that play a key role in regulation of folliculogenesis, oocyte function and early embryonic development, and to identify objective molecular markers that are predictive of oocyte competence and subsequent potential reproductive success post-fertilization.

Keywords

COS Keywords:

Animal Science, Genomics, Molecular Biology, Reproductive Physiology, Transgenics.

Additional Terms:

Embryo, Genomics, Oocyte, Ovarian Function, Reproductive Physiology, Stress.

Memberships

American Association for the Advancement of Science
Endocrine Society
Society for the Study of Reproduction

Previous Positions

1999-2003, Adjunct Assistant Professor, Michigan State University, College of Natural Science, Physiology
1997-2003, Assistant Professor, Michigan State University, College of Agriculture & Natural Resources, Animal Science
1996-1997, Postdoctoral Fellow, Clayton Foundation Laboratories for Peptide Biology, The Salk Institute

Funding Received

  • United States Department of Agriculture (USDA): Role of Matrix Metalloproteinases in Ovulation, $155,000, Sep 1998 to Aug 2000.
  • United States Department of Agriculture (USDA): Neuroendocrine regulation of the stress response in cattle, $260,000, Oct 2001 to Sep 2004.
  • United States Department of Agriculture (USDA): Intrafollicular signaling pathways that control follicle rupture in dairy cattle, $267,000, Dec 2002 to Nov 2005.

Publications

  • Zielak AE, Canty MJ, Forde N, Coussens PM, Smith GW, Lonergan P, Ireland JJ, Evans AC (May 2008) Differential expression of genes for transcription factors in theca and granulosa cells following selection of a dominant follicle in cattle., Molecular reproduction and development, 75 (5), 904-14 Abstract
  • Forde N, Rogers M, Canty MJ, Lonergan P, Smith GW, Coussens PM, Ireland JJ, Evans AC (Feb 2008) Association of the prion protein and its expression with ovarian follicle development in cattle., Molecular reproduction and development, 75 (2), 243-9 Abstract
  • Bettegowda A, Lee KB, Smith GW (2008) Cytoplasmic and nuclear determinants of the maternal-to-embryonic transition., Reproduction, fertility, and development, 20 (1), 45-53 Abstract
  • Bettegowda A, Yao J, Sen A, Li Q, Lee KB, Kobayashi Y, Patel OV, Coussens PM, Ireland JJ, Smith GW (Nov 2007) JY-1, an oocyte-specific gene, regulates granulosa cell function and early embryonic development in cattle., Proceedings of the National Academy of Sciences of the United States of America, 104 (45), 17602-7 Abstract
  • Knights M, Smith GW (Nov 2007) Decreased ACTH secretion during prolonged transportation stress is associated with reduced pituitary responsiveness to tropic hormone stimulation in cattle., Domestic animal endocrinology, 33 (4), 442-50 Abstract
  • Sen A, Bettegowda A, Jimenez-Krassel F, Ireland JJ, Smith GW (Sep 2007) Cocaine- and amphetamine-regulated transcript regulation of follicle-stimulating hormone signal transduction in bovine granulosa cells., Endocrinology, 148 (9), 4400-10 Abstract
  • Ireland JJ, Ward F, Jimenez-Krassel F, Ireland JL, Smith GW, Lonergan P, Evans AC (Jun 2007) Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle., Human reproduction (Oxford, England), 22 (6), 1687-95 Abstract
  • Li Q, Jimenez-Krassel F, Bettegowda A, Ireland JJ, Smith GW (Mar 2007) Evidence that the preovulatory rise in intrafollicular progesterone may not be required for ovulation in cattle., The Journal of endocrinology, 192 (3), 473-83 Abstract
  • Smith GW, Rosa GJ (Mar 2007) Interpretation of microarray data: trudging out of the abyss towards elucidation of biological significance., Journal of animal science, 85 (13 Suppl), E20-3 Abstract
  • Bettegowda A, Smith GW (2007) Mechanisms of maternal mRNA regulation: implications for mammalian early embryonic development., Frontiers in bioscience : a journal and virtual library, 12, 3713-26 Abstract
  • Bettegowda A, Smith GW (2007) Mechanisms of maternal mRNA regulation: implications for mammalian early embryonic development., Frontiers in bioscience : a journal and virtual library, 12, 3713-26 Abstract
  • Patel OV, Bettegowda A, Ireland JJ, Coussens PM, Lonergan P, Smith GW (Jan 2007) Functional genomics studies of oocyte competence: evidence that reduced transcript abundance for follistatin is associated with poor developmental competence of bovine oocytes., Reproduction (Cambridge, England), 133 (1), 95-106 Abstract
  • Saama PM, Patel OV, Bettegowda A, Ireland JJ, Smith GW (Dec 2006) Novel algorithm for transcriptome analysis., Physiological genomics, 28 (1), 62-6 Abstract
  • Mihm M, Baker PJ, Ireland JL, Smith GW, Coussens PM, Evans AC, Ireland JJ (Jun 2006) Molecular evidence that growth of dominant follicles involves a reduction in follicle-stimulating hormone dependence and an increase in luteinizing hormone dependence in cattle., Biology of reproduction, 74 (6), 1051-9 Abstract
  • Corcoran D, Fair T, Park S, Rizos D, Patel OV, Smith GW, Coussens PM, Ireland JJ, Boland MP, Evans AC, Lonergan P (Apr 2006) Suppressed expression of genes involved in transcription and translation in in vitro compared with in vivo cultured bovine embryos., Reproduction (Cambridge, England), 131 (4), 651-60 Abstract
  • Li Q, Jimenez-Krassel F, Kobayashi Y, Ireland JJ, Smith GW (Mar 2006) Effect of intrafollicular indomethacin injection on gonadotropin surge-induced expression of select extracellular matrix degrading enzymes and their inhibitors in bovine preovulatory follicles., Reproduction (Cambridge, England), 131 (3), 533-43 Abstract
  • Bettegowda A, Patel OV, Ireland JJ, Smith GW (Mar 2006) Quantitative analysis of messenger RNA abundance for ribosomal protein L-15, cyclophilin-A, phosphoglycerokinase, beta-glucuronidase, glyceraldehyde 3-phosphate dehydrogenase, beta-actin, and histone H2A during bovine oocyte maturation and early embryogen, Molecular reproduction and development, 73 (3), 267-78 Abstract
  • Kobayashi Y, Jimenez-Krassel F, Ireland JJ, Smith GW (2006) Evidence of a local negative role for cocaine and amphetamine regulated transcript (CART), inhibins and low molecular weight insulin like growth factor binding proteins in regulation of granulosa cell estradiol production during follicular waves in cattle, Reproductive biology and endocrinology : RB&E, 4, 22 Abstract
  • Patel OV, Suchyta SP, Sipkovsky SS, Yao J, Ireland JJ, Coussens PM, Smith GW (May 2005) Validation and application of a high fidelity mRNA linear amplification procedure for profiling gene expression., Veterinary immunology and immunopathology, 105 (3-4), 331-42 Abstract
  • Li Q, Bakke LJ, Pursley JR, Smith GW (Nov 2004) Localization and temporal regulation of tissue inhibitors of metalloproteinases 3 and 4 in bovine preovulatory follicles., Reproduction (Cambridge, England), 128 (5), 555-64 Abstract
  • Kobayashi Y, Jimenez-Krassel F, Li Q, Yao J, Huang R, Ireland JJ, Coussens PM, Smith GW (Nov 2004) Evidence that cocaine- and amphetamine-regulated transcript is a novel intraovarian regulator of follicular atresia., Endocrinology, 145 (11), 5373-83 Abstract
  • Yao J, Ren X, Ireland JJ, Coussens PM, Smith TP, Smith GW (Sep 2004) Generation of a bovine oocyte cDNA library and microarray: resources for identification of genes important for follicular development and early embryogenesis., Physiological genomics, 19 (1), 84-92 Abstract
  • Bakke LJ, Li Q, Cassar CA, Dow MP, Pursley JR, Smith GW (Aug 2004) Gonadotropin surge-induced differential upregulation of collagenase-1 (MMP-1) and collagenase-3 (MMP-13) mRNA and protein in bovine preovulatory follicles., Biology of reproduction, 71 (2), 605-12 Abstract
  • Peters MW, Pursley JR, Smith GW (Jun 2004) Inhibition of intrafollicular PGE2 synthesis and ovulation following ultrasound-mediated intrafollicular injection of the selective cyclooxygenase-2 inhibitor NS-398 in cattle., Journal of animal science, 82 (6), 1656-62 Abstract
  • Weber PS, Toelboell T, Chang LC, Tirrell JD, Saama PM, Smith GW, Burton JL, Mechanisms of glucocorticoid-induced down-regulation of neutrophil L-selectin in cattle: Evidence for effects at the gene-expression level and primarily on blood neutrophils, Journal of Leukocyte Biology, 75(5), 815-27, May 2004
  • Veney SL, Peabody C, Smith GW, Wade J, Sexually dimorphic neurocalcin expression in the developing zebra finch telencephalon, Journal of Neurobiology, 56(4), 372-86, September 2003 Abstract
  • Silva LF, VandeHaar MJ, Weber Nielsen MS, Smith GW, Evidence for a local effect of leptin in bovine mammary gland, Journal of Dairy Science, 85(12), 3277-86, December 2002 Abstract
  • Vetter DE, Li C, Zhao L, Contarino A, Liberman MC, Smith GW, Marchuk Y, Koob GF, Heinemann SF, Vale W, Lee KF, Urocortin-deficient mice show hearing impairment and increased anxiety-like behavior, Nature Genetics, 31(4), 363-9, Aug 2002 Abstract
  • Qahwash IM, Cassar CA, Radcliff RP, Smith GW, Bacterial lipopolysaccharide-induced coordinate downregulation of arginine vasopressin receptor V3 and corticotropin-releasing factor receptor 1 messenger ribonucleic acids in the anterior, Endocrine, 18(1), 13-20, June 2002 Abstract
  • Bakke LJ, Dow MP, Cassar CA, Peters MW, Pursley JR, Smith GW, Effect of the preovulatory gonadotropin surge on matrix metalloproteinase (MMP)-14, MMP-2, and tissue inhibitor of metalloproteinases-2 expression within bovine periovulatory follicular an, Biology of Reproduction, 66(6), 1627-34, June 2002 Abstract
  • Ricke WA, Smith GW, McIntush EW, Smith MF, Analysis of luteal tissue inhibitor of metalloproteinase-1, -2, and -3 during prostaglandin F(2alpha)-induced luteolysis, Biology of Reproduction, 66(5), 1387-94, May 2002 Abstract
  • Cassar CA, Dow MP, Pursley JR, Smith GW, Effect of the preovulatory LH surge on bovine follicular progesterone receptor mRNA expression, Domestic Animal Endocrinology, 22(3), 179-87, May 2002 Abstract
  • Smith MF, Ricke WA, Bakke LJ, Dow MP, Smith GW, Ovarian tissue remodeling: Role of matrix metalloproteinases and their inhibitors, Molecular and Cellular Endocrinology, 191(1), 45-56, May 2002
  • Dow MP, Bakke LJ, Cassar CA, Peters MW, Pursley JR, Smith GW, Gonadotrophin surge-induced upregulation of mRNA for plasminogen activator inhibitors 1 and 2 within bovine periovulatory follicular and luteal tissue, Reproduction (Cambridge, England), 123(5), 711-9, May 2002
  • Ricke WA, Smith GW, Reynolds LP, Redmer DA, Smith MF, Matrix metalloproteinase (2, 9, and 14) expression, localization, and activity in ovine corpora lutea throughout the estrous cycle, Biology of Reproduction, 66(4), 1083-94, April 2002 Abstract
  • Peters MW, Smith MF, Smith GW, Use of critical interactive thinking exercises in teaching reproductive physiology to undergraduate students, Journal of Animal Science, 80(3), 862-5, March 2002 Abstract
  • Ricke WA, Smith GW, Smith MF, Matrix metalloproteinase expression and activity following prostaglandin F(2 alpha)-induced luteolysis, Biology of Reproduction, 66(3), 685-91, March 2002 Abstract
  • Weber PS, Madsen SA, Smith GW, Ireland JJ, Burton JL, Pre-translational regulation of neutrophil L-selectin in glucocorticoid-challenged cattle, Veterinary Immunology and Immunopathology, 83(3-4), 213-40, December 2001 Abstract
  • Lee S, Smith GW, Vale W, Lee KF, Rivier C, Mice that lack corticotropin-releasing factor (CRF) receptors type 1 show a blunted ACTH response to acute alcohol despite up-regulated constitutive hypothalamic CRF gene expression, Alcoholism, Clinical and Experimental Research, 25(3), 427-33, March 2001 Abstract
  • Contarino A, Dellu F, Koob GF, Smith GW, Lee KF, Vale WW, Gold LH, Dissociation of locomotor activation and suppression of food intake induced by CRF in CRFR1-deficient mice, Endocrinology, 141(7), 2698-702, July 2000 Abstract
  • Bale TL, Contarino A, Smith GW, Chan R, Gold LH, Sawchenko PE, Koob GF, Vale WW, Lee KF, Mice deficient for corticotropin-releasing hormone receptor-2 display anxiety-like behaviour and are hypersensitive to stress, Nature Genetics, 24(4), 410-4, April 2000 Abstract
  • Contarino A, Dellu F, Koob GF, Smith GW, Lee KF, Vale W, Gold LH, Reduced anxiety-like and cognitive performance in mice lacking the corticotropin-releasing factor receptor 1, Brain Research, 835(1), 1-9, July 1999 Abstract
  • Turnbull AV, Smith GW, Lee S, Vale WW, Lee KF, Rivier C, CRF type I receptor-deficient mice exhibit a pronounced pituitary-adrenal response to local inflammation, Endocrinology, 140(2), 1013-7, February 1999 Abstract
  • Smith MF, McIntush EW, Ricke WA, Kojima FN, Smith GW, Regulation of ovarian extracellular matrix remodelling by metalloproteinases and their tissue inhibitors: Effects on follicular development, ovulation and luteal function, Journal of Reproduction and Fertility. Supplement, 54, 367-81, 1999
  • Gentry PC, Smith GW, Leighr DR, Bao B, Smith MF, Ontogeny of stem cell factor receptor (c-kit) messenger ribonucleic acid in the ovine corpus luteum, Biology of Reproduction, 59(4), 983-90, October 1998 Abstract
  • Smith GW, Aubry JM, Dellu F, Contarino A, Bilezikjian LM, Gold LH, Chen R, Marchuk Y, Hauser C, Bentley CA, Sawchenko PE, Koob GF, Vale W, Lee KF, Corticotropin releasing factor receptor 1-deficient mice display decreased anxiety, impaired stress response, and aberrant neuroendocrine development, Neuron, 20(6), 1093-102, June 1998 Abstract
  • Zhao L, Donaldson CJ, Smith GW, Vale W, The structures of the mouse and human urocortin genes, Genomics, 50, 23-33, 1998
  • Bao B, Garverick H A, Smith G W, Smith M F, Salfen B E, Youngquist R S, Expression of messenger ribonucleic acid (mRNA) encoding 3beta-hydroxysteroid dehydrogenase delta4,delta5 isomerase (3beta-HSD) during recruitment and selection of bovine ovarian follicles: Identifica, Biology of Reproduction, 56(6), 1466-73, June 1997
  • Bao B, Garverick HA, Smith GW, Smith MF, Salfen BE, Youngquist RS, Changes in messenger ribonucleic acid encoding luteinizing hormone receptor, cytochrome P450-side chain cleavage, and aromatase are associated with recruitment and selection of bovine ovarian follicle, Biology of Reproduction, 56(5), 1158-68, May 1997 Abstract
  • Smith GW, Gentry PC, Bao B, Long DK, Roberts RM, Smith MF, Control of extracellular matrix remodelling within ovarian tissues: Localization and regulation of gene expression of plasminogen activator inhibitor type-1 within the ovine corpus luteum, Journal of Reproduction and Fertility, 110(1), 107-14, May 1997
  • Gentry PC, Smith GW, Anthony RV, Zhang Z, Long DK, Smith MF, Characterization of ovine stem cell factor messenger ribonucleic acid and protein in the corpus luteum throughout the luteal phase, Biology of Reproduction, 54(5), 970-9, May 1996 Abstract
  • McIntush EW, Pletz JD, Smith GW, Long DK, Sawyer HR, Smith MF, Immunolocalization of tissue inhibitor of metalloproteinases-1 within ovine periovulatory follicular and luteal tissues, Biology of Reproduction, 54(4), 871-8, April 1996 Abstract
  • Smith GW, Juengel JL, Mclntush EW, Youngquist RS, Garverick HA, Smith MF, Ontogenies of messenger RNA encoding tissue inhibitor of metalloproteinases 1 and 2 within bovine periovulatory follicles and luteal tissue, Domestic Animal Endocrinology, 13(2), 151-60, March 1996 Abstract
  • Smith GW, Gentry PC, Long DK, Smith MF, Roberts RM, Differential gene expression within the ovine corpus luteum: Identification of secreted protein acidic and rich in cysteine as a major secretory product of small but not large luteal cells, Endocrinology, 137(2), 755-62, February 1996
  • Smith GW, Gentry PC, Roberts RM, Smith MF, Ontogeny and regulation of luteinizing hormone receptor messenger ribonucleic acid within the ovine corpus luteum, Biology of Reproduction, 54(1), 76-83, January 1996 Abstract
  • Xu Z, Garverick HA, Smith GW, Smith MF, Hamilton SA, Youngquist RS, Expression of follicle-stimulating hormone and luteinizing hormone receptor messenger ribonucleic acids in bovine follicles during the first follicular wave, Biology of Reproduction, 53(4), 951-7, October 1995 Abstract
  • Xu Z, Garverick HA, Smith GW, Smith MF, Hamilton SA, Youngquist RS, Expression of messenger ribonucleic acid encoding cytochrome P450 side-chain cleavage, cytochrome p450 17 alpha-hydroxylase, and cytochrome P450 aromatase in bovine follicles during the first follicul, Endocrinology, 136(3), 981-9, March 1995 Abstract
  • Smith GW, McCrone S, Petersen SL, Smith MF, Expression of messenger ribonucleic acid encoding tissue inhibitor of metalloproteinases-2 within ovine follicles and corpora lutea, Endocrinology, 136(2), 570-6, February 1995 Abstract
  • Roberts RM, Mathialagan N, Duffy JY, Smith GW, Regulation and regulatory role of proteinase inhibitors, Critical Reviews in Eukaryotic Gene Expression, 5(3-4), 385-436, 1995 Abstract
  • Anthony RV, Smith GW, Duong A, Pratt SL, Smith MF, Identification of two forms of prolactin receptor mRNA in ovine fetal liver and adult ovary, Endocrine, 3, 291-295, 1995
  • Smith M F, McIntush E W, Smith G W, Mechanisms associated with corpus luteum development., Journal of Animal Science, 72(7), 1857-72, July 1994 Abstract
  • Smith M F, Kemper C N, Smith G W, Goetz T L, Jarrell V L, Production of tissue inhibitor of metalloproteinases-1 by porcine follicular and luteal cells, Journal of Animal Science, 72(4), 1004-12, April 1994
  • Juengel J L, Smith G W, Smith M F, Youngquist R S, Garverick H A, Pattern of protein production by bovine corpora lutea during luteolysis and characterization of expression of two major secretory products of regressing corpora lutea, Journal of Reproduction and Fertility, 100(2), 515-20, March 1994
  • Smith G W, Goetz T L, Anthony R V, Smith M F, Molecular cloning of an ovine ovarian tissue inhibitor of metalloproteinases: Ontogeny of messenger ribonucleic acid expression and in situ localization within preovulatory follicles and luteal tissue, Endocrinology, 134(1), 344-52, January 1994
  • Smith G W, Moor R M, Smith M F, Identification of a 30,000 M(r) polypeptide secreted by cultured ovine granulosa cells and luteal tissue as a tissue inhibitor of metalloproteinases, Biology of Reproduction, 48(1), 125-32, January 1993

Profile Details

Last Updated: 3/11/2008

COS Expertise ID #444682
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