Dr. Joannes B. Hoek

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Thomas Jefferson University
Jefferson Medical College
Pathology, Anatomy & Cell Biology
Cell Biology
Professor
Professional Headshot of Joannes B. Hoek

Mailing Address

Thomas Jefferson University
Dept of Pathology, Anatomy and Cell Biology
Rm 269 JAH
1020 Locust Street
Philadelphia, Pennsylvania 19107-5587
United States

Contact Information

Phone: (215) 503-5016
Fax: (215) 923-2218
Jan.Hoek@jefferson.edu

Qualifications

Ph.D..

Expertise and Research Interests

Research in my laboratory is focused on the analysis of integrated signaling responses in liver and other tissues and on the adaptive or maladaptive modification of these responses by acute and chronic ethanol exposure. The following project are currently ongoing:
- Analysis of growth factor signaling networks and related gene expression profiles in liver, using a combination of computational modeling and experimental studies
- Signaling events associated with the onset and progression of liver regeneration, with emphasis on the control of energy metabolism and AMP-activated protein kinase
- Mechanisms of ethanol-associated disturbance of cellular Ca2+ homeostasis and Ca2+ signaling and its role in adaptation and stress-responses in the liver
- Ethanol-induced changes in mitochondrial energy conservation, oxidative stress, and damage to mtDNA

Keywords

COS Keywords:

Alcohol Or Alcoholism, Alcoholism Prevention, Alcoholism Treatment, Cell Culture, Cytokines, Cytoskeleton, DNA Replication, Growth Factor Receptors, Growth Factors, Laboratory Animal Science, Microscopy, Signal Transduction, Tissue Culture.

Additional Terms:

Biological Signal Transduction, Calcium Flux, Cytokine, Cytoskeleton, DNA Replication, Diacylglycerol, Digital Imaging, Epidermal Growth Factor, Ethanol, Fluorescence Microscopy, G Protein, Growth Factor, Growth Factor Receptor, Inositol Phosphate, Laboratory Rat, Liver Regeneration, Mitogen Activated Protein Kinase, Protein Kinase A, Protein Kinase C, Protein Tyrosine Kinase, Systems Biology, Tissue Cell Culture, Transforming Growth Factor.

Funding Received

  • R24 AA014986: Core Facilities to Support Alcohol Research, 2005 to 2010.
  • T32 AA07463: Alcoholic Tissue Injury, 2005 to 2010.
  • R01 AA015311: Effect of chronic ethanol treatment on Ca2+-mediated signal-response coupling in the liver, 2004 to 2009.
  • R01 AA08714: Alcohol and the control of liver regeneration., 1991 to present.

Publications

  • Borisov, N.M., Markevich, N.I., Hoek, J.B. and Kholodenko, B.N. (2006) Trading the micro-world of combinatorial complexity for the macro-world of protein interaction domains, Biosystems, 83, 152-166
  • Covarrubias, M.Y., Khan, R.L., Vadigepalli, R., Hoek, J.B. and Schwaber, J.S. (2005) Chronic alcohol exposure alters transcription broadly in a key integrative nucleus for homeostasis: the nucleus tractus solitarius., Physiological Genomics, 24, 45-58
  • Pastorino,J.G., Hoek,J.B. and Shulga, N. (2005) Activation of glycogen synthase kinase 3-beta disrupts the binding of hexokinase II to mitochondria by phosphorylating voltage-dependent anion channel and potentiates chemotherapy-induced cytotoxicity., Cancer Research, 65, 10545-10554
  • Borisov NM, Markevich NI, Hoek JB, Kholodenko BN (Aug 2005) Signaling through receptors and scaffolds: independent interactions reduce combinatorial complexity., Biophysical Journal, 89 (2), 951-66 Abstract
  • Hajnoczky G, Buzas CJ, Pacher P, Hoek JB, Rubin E (May 2005) Alcohol and mitochondria in cardiac apoptosis: mechanisms and visualization., Alcoholism, Clinical and Experimental Research, 29 (5), 693-701 Abstract
  • Shulga N, Hoek JB, Pastorino JG (Mar 2005) Elevated PTEN levels account for the increased sensitivity of ethanol-exposed cells to tumor necrosis factor-induced cytotoxicity., The Journal of Biological Chemistry, 280 (10), 9416-24 Abstract
  • Rubin R, Harrison R, Chen XF, Corzitotto J, Hoek JB, Hallak H (Nov 2004) Inhibition of insulin-like growth factor I receptor tyrosine kinase by ethanol., Biochemical Pharmacology, 68 (10), 2009-17 Abstract
  • Hoek JB, Pastorino JG, Cellular signaling mechanisms in alcohol-induced hepatic injury, Seminars in Liver Disease, 24(3), 257-272, August 2004
  • Markevich NI, Hoek JB, Kholodenko BN, Signaling switches and bistability arising from multisite phosphorylation in protein kinase cascades, Journal of Cell Biology, 164(3), 353-9, February 2004 Abstract
  • Suenaga A, Kiyatkin AB, Hatakeyama M, Futatsugi N, Okimoto N, Hirano Y, Narumi T, Kawai A, Susukita R, Koishi T, Furusawa H, Yasuoka K, Takada N, Ohno Y, Taiji M, Ebisuzaki T, Hoek JB, Konagaya A, Kholodenko BN, Tyr-317 phosphorylation increases Shc structural rigidity and reduces coupling of domain motions remote from the phosphorylation site as revealed by molecular dynamics simulations, Journal of Bioligical Chemistry, 279(6), 4657-62, February 2004 Abstract
  • Pastorino JG, Shulga N, Hoek JB, TNF-alpha-induced cell death in ethanol-exposed cells depends on p38 MAPK signaling but is independent of Bid and caspase-8, American Journal of Physiology. Gastrointestinal and Liver Physiology, 285(3), G503-16, September 2003 Abstract
  • Pastorino JG, Hoek JB, Hexokinase II: the integration of energy metabolism and control of apoptosis, Current Medicinal Chemistry, 10(16), 1535-51, August 2003 Abstract
  • Hallak H, Moehren G, Tang J, Kaou M, Addas M, Hoek JB, Rubin R, Epidermal growth factor-induced activation of the insulin-like growth factor I receptor in rat hepatocytes, Hepatology (baltimore, Md.), 36(6), 1509-18, December 2002 Abstract
  • Kholodenko BN, Kiyatkin A, Bruggeman FJ, Sontag E, Westerhoff HV, Hoek JB, Untangling the wires: a strategy to trace functional interactions in signaling and gene networks, Proceedings of the National Academy of Sciences of the United States of America., 99(20), 12841-6, October 2002 Abstract
  • Bruggeman FJ, Westerhoff HV, Hoek JB, Kholodenko BN (Oct 2002) Modular response analysis of cellular regulatory networks., Journal of Theoretical Biology, 218 (4), 507-20 Abstract
  • Nesti LJ, Caterson EJ, Wang M, Chang R, Chapovsky F, Hoek JB, Tuan RS, TGF-beta1 calcium signaling increases alpha5 integrin expression in osteoblasts, Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society., 20(5), 1042-9, September 2002 Abstract
  • Pusl T, Wu JJ, Zimmerman TL, Zhang L, Ehrlich BE, Berchtold MW, Hoek JB, Karpen SJ, Nathanson MH, Bennett AM, Epidermal growth factor-mediated activation of the ETS domain transcription factor Elk-1 requires nuclear calcium, Journal of Bioligical Chemistry, 277(30), 27517-27, July 2002 Abstract
  • Hoek JB, Cahill A, Pastorino JG, Alcohol and mitochondria: a dysfunctional relationship, Gastroenterology, 122(7), 2049-63, June 2002 Abstract
  • Nesti LJ, Caterson EJ, Wang M, Chang R, Chapovsky F, Hoek JB, Tuan RS (Jun 2002) TGF-beta1-stimulated osteoblasts require intracellular calcium signaling for enhanced alpha5 integrin expression., Annals of the New York Academy of Sciences, 961, 178-82 Abstract
  • Hoek JB, Pastorino JG, Ethanol, oxidative stress, and cytokine-induced liver cell injury, Alcohol (fayetteville, N.y.), 27(1), 63-8, May 2002 Abstract
  • Pastorino JG, Shulga N, Hoek JB (Mar 2002) Mitochondrial binding of hexokinase II inhibits Bax-induced cytochrome c release and apoptosis., The Journal of Biological Chemistry, 277 (9), 7610-8 Abstract
  • Moehren G, Markevich N, Demin O, Kiyatkin A, Goryanin I, Hoek JB, Kholodenko BN (Jan 2002) Temperature dependence of the epidermal growth factor receptor signaling network can be accounted for by a kinetic model., Biochemistry, 41 (1), 306-20 Abstract
  • Donahue RJ, Razmara M, Hoek JB, Knudsen TB, Direct influence of the p53 tumor suppressor on mitochondrial biogenesis and function, Faseb Journal, 15(3), 635-44, March 2001 Abstract
  • Nojiri S, Hoek JB, Suppression of epidermal growth factor-induced phospholipase C activation associated with actin rearrangement in rat hepatocytes in primary culture, Hepatology, 32(5), 947-57, November 2000 Abstract
  • Kholodenko BN, Brown GC, Hoek JB, Diffusion control of protein phosphorylation in signal transduction pathways, Biochemical Journal, 350 Pt 3, 901-7, 2000 Abstract
  • Kholodenko BN, Brown GC, Hoek JB (Sep 2000) Diffusion control of protein phosphorylation in signal transduction pathways., The Biochemical Journal, 350 Pt 3, 901-7 Abstract
  • Marcinkeviciute A, Mildaziene V, Crumm S, Demin O, Hoek JB, Kholodenko B, Kinetics and control of oxidative phosphorylation in rat liver mitochondria after chronic ethanol feeding, Biochemical Journal, 349(Pt 2), 519-26, 2000 Abstract
  • Wu J, Liu SL, Zhu JL, Norton PA, Nojiri S, Hoek JB, Zern MA (Jul 2000) Roles of tissue transglutaminase in ethanol-induced inhibition of hepatocyte proliferation and alpha 1-adrenergic signal transduction., The Journal of Biological Chemistry, 275 (29), 22213-9 Abstract
  • Miro O, Robert J, Casademont J, Alonso JR, Nicolas JM, Fernandez-Sola J, Urbano-Marquez A, Hoek JB, Cardellach F (Jun 2000) Heart mitochondrial respiratory chain complexes are functionally unaffected in heavy ethanol drinkers without cardiomyopathy., Alcoholism, Clinical and Experimental Research, 24 (6), 859-64 Abstract
  • Kholodenko BN, Hoek JB, Westerhoff HV (May 2000) Why cytoplasmic signalling proteins should be recruited to cell membranes., Trends in Cell Biology, 10 (5), 173-8 Abstract
  • Pastorino JG, Hoek JB (May 2000) Ethanol potentiates tumor necrosis factor-alpha cytotoxicity in hepatoma cells and primary rat hepatocytes by promoting induction of the mitochondrial permeability transition., Hepatology (baltimore, Md.), 31 (5), 1141-52 Abstract

Profile Details

Last Updated: 5/5/2006

COS Expertise ID #536603
Reference this profile directly: http://myprofile.cos.com/Jan.Hoek