Dr. Paul E. Neiman |
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Fred Hutchinson Cancer Research Center Basic Sciences Member and Director EmeritusAppointed: 2004 University of Washington School of Medicine Medicine Oncology Professor EmeritusAppointed: 2005 Fred Hutchinson Cancer Research Center Human Biology Member EmeritusAppointed: 2004 |  |
Mailing AddressFred Hutchinson Cancer Research Center 1100 Fairview Avenue N. P.O. Box 19024 C2-023 Seattle, Washington 98109-1024United States | Contact Information |
Qualifications
M.D., University of Washington, 1964.
Expertise and Research Interests
Cellular and viral oncogenes
Normal and neoplastic B-cell development in the bursa of Fabricius
Functional genomic analysis of somatic genomic instability in cancer
The research in the Neiman laboratory, over the years, focussed generally on retroviruses and on normal and neoplastic development in the B-cell component of the immune system. The principal experimental system we exploited is the bursa of Fabricius in chickens. We demonstrated that bursal stem cells are selective targets in the B-cell lineage for the action of Myc oncogenes. Introduction of deregulated myc oncogenes into bursal stem cells in a transplantation assay developed in this laboratory, results in the proliferation of preneoplastic cells that are arrested at the bursal stem-cell stage of B-cell differentiation. Our work has included analysis of apoptotic
cell death in the bursa, which characterized little-studied Bcl-2 family members controlling bursal stem cell survival and follicular development. We developed tools for functional genomic analysis in this system and compared transcriptional signatures of normal embryonic bursal development with the preneoplastic and neoplastic stages of myc-induced lymphomagenesis. These studies demonstrated large neoplasia-associated transcriptional change, and a strong effect of the level of myc oncogene expression on the pattern of transcription. Comparison of bursal lymphomagenesis induced by Myc characterized similar neoplasms induced by two other transcription factors c-myb and v-rel. More recently, using array comparative genomic hybridiztion (Array CGH) and genome-wide analysis of pallindrome formation (GAPF), we examined aspects of genetic instabilty during Myc-induced lymphomagenesis . We discovered extensive DNA copy number change, principally amplification, and palindrome formation at early preneoplastic stages of tumorigenesis.
Currently, experimental work in the Neiman laboratory is being phased out. Dr. Neiman continues his interests through collaborative research with the Tapscott laboratory in Human Biology at FHCRC exploring GAPF-related technology for detecting genome-wide DNA palindrome formation and hyper methylation during human tumorigenesis
Normal and neoplastic B-cell development in the bursa of Fabricius
Functional genomic analysis of somatic genomic instability in cancer
The research in the Neiman laboratory, over the years, focussed generally on retroviruses and on normal and neoplastic development in the B-cell component of the immune system. The principal experimental system we exploited is the bursa of Fabricius in chickens. We demonstrated that bursal stem cells are selective targets in the B-cell lineage for the action of Myc oncogenes. Introduction of deregulated myc oncogenes into bursal stem cells in a transplantation assay developed in this laboratory, results in the proliferation of preneoplastic cells that are arrested at the bursal stem-cell stage of B-cell differentiation. Our work has included analysis of apoptotic
cell death in the bursa, which characterized little-studied Bcl-2 family members controlling bursal stem cell survival and follicular development. We developed tools for functional genomic analysis in this system and compared transcriptional signatures of normal embryonic bursal development with the preneoplastic and neoplastic stages of myc-induced lymphomagenesis. These studies demonstrated large neoplasia-associated transcriptional change, and a strong effect of the level of myc oncogene expression on the pattern of transcription. Comparison of bursal lymphomagenesis induced by Myc characterized similar neoplasms induced by two other transcription factors c-myb and v-rel. More recently, using array comparative genomic hybridiztion (Array CGH) and genome-wide analysis of pallindrome formation (GAPF), we examined aspects of genetic instabilty during Myc-induced lymphomagenesis . We discovered extensive DNA copy number change, principally amplification, and palindrome formation at early preneoplastic stages of tumorigenesis.
Currently, experimental work in the Neiman laboratory is being phased out. Dr. Neiman continues his interests through collaborative research with the Tapscott laboratory in Human Biology at FHCRC exploring GAPF-related technology for detecting genome-wide DNA palindrome formation and hyper methylation during human tumorigenesis
Keywords
COS Keywords:
Apoptosis, Cancer Biology, Hematology, Oncogenes, Oncology, Retrovirus, Stem Cells, Tumors.Additional Terms:
B-Cell Development, Bursa of Fabricius, Somatic Stem Cells, Tumor Biology.Previous Positions
1983-1996, Director,
Fred Hutchinson Cancer Research Center,
Basic Sciences
1982-2004, Adjunct Professor,
University of Washington,
School of Medicine,
Pathology
1980-1983, Acting Scientific Director,
Fred Hutchinson Cancer Research Center
1978-2004, Member,
Fred Hutchinson Cancer Research Center,
Basic Sciences
1978-1999, Member,
Fred Hutchinson Cancer Research Center,
Clinical Research
1978-2004, Professor,
University of Washington,
School of Medicine,
Internal Medicine,
Oncology
1971-1978, Assistant and Associate Professor,
University of Washington,
School of Medicine,
Internal Medicine,
Oncology
1968-1971, Fellow,
University of Washington,
School of Medicine,
Internal Medicine,
Hematology and Oncology
1966-1968, Clinical Associate,
National Cancer Institute
1964-1966, Resident,
Case Western Reserve University,
School of Medicine,
Internal Medicine
Publications
- Paul E. Neiman, Katrina Elsaesser, Gilbert Loring, Robert Kimmel (2008) Myc Oncogene-Induced Genomic Instability: DNA Palindromes in Bursal Lymphomagenesis., Plos Genetics, 4 (7), e1000132 doc10.1371
- Neiman PE, Kimmel, R, Icreversi, A, Elsaesser, K, Bowers, SJ, Burnside, J, Delrow, J (2006) Genomic Instability during Myc-induced neoplastic development in the bursa of Fabricius, Oncogene, 25, 6325-633
- Burnside, J., Neiman, P.E, Aronszajn, M, Burt, D., Talbot, R, Delrow, J, Development of a CDNA Array for Chicken Gene Expression Analysis., BMC Genomics, 6, 13, 2005
- Parghi, S.S., Brandvold, K.A.,, Bowers, S.J, Neiman, P.E, Ruddell, A, Reduced Myc Overexpression and Normal B-cell Differentiation Mediate Resistance to Avian Leukosis Virus Lymphomagenesis., Oncogene, 23, 4413 - 4421, 2004
- Brown, C.Y., Bowers, S.J., Loring, G, Heberden, C., Lee, R-L., Neiman, P.E., Role of Mtd/Bok in Normal and Neoplastic B-cell Development in the Bursa of Fabricius., Developmental and Comparative Immunology, 28, 619-634, 2004
- Paul E. Neiman, Jovana J. Grbic, Tatjana S. Polony, Robert Kimmel, Sandra J Bowers, Jeffrey Delrow, Karen Beemon, Functional Genomic Analysis Reveals Distinct Neoplastic Phenotypes Associated With C-myb Mutation in the Bursa of Fabricius, Oncogene, 22, 1073-1086, 2003
- Paul E Neiman, Alanna Ruddell, Christine Jasoni, Gil Loring, Sandra Jo Thomas, Kimberly A. Brandvold, Ruey-min Lee, Joan Burnside, Jeffrey Delrow, Analysis of gene expression during myc oncogene-induced lymphomagenesis in the bursa of Fabricius, Proceedings of the National Academy of Sciences (USA), 98(11), 6378-6383, 22 May 2001
- Kimberly A Brandvold, Donald L Ewart, Sally Kent, Paul Neiman, Alanna Ruddell, Blocked B cell differentiation and emigration support early growth of Myc-induced lymphomas, Oncogene, 20, 3226-3234, 2001
- Lee, R-M, Germain, G, Burnside, J, Thomas, SJ. and Neiman P., The role of Nr13 in regulation of programmed cell death in the bursa of Fabricius, Genes and Development, 13, 718-728, 1999
Profile Details
Last Updated: 8/6/2009
COS Expertise ID #135372
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