Dr. Vyacheslav Andrianov

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Thomas Jefferson University
Jefferson Medical College
Cancer Biology
Biotechnology Foundation Laboratories/IBAMM
Research Assistant ProfessorAppointed: 2000
Professional Headshot of Vyacheslav Andrianov

Mailing Address

Jefferson Center at Delaware Valley College
700 East Butler Ave.
Philadelphia, Pennsylvania 19107-5587
United States

Contact Information

Phone: (215) 489-4926
Fax: (215) 489-4941
Vyacheslav.Andrianov@jefferson.edu

Qualifications

Ph.D..

Expertise and Research Interests

Experienced in most modern techniques for gene transfer and expression in plants.

In 1974-79 first in the USSR constructed a genomic library for plant (pea) chloroplast DNA in E.coli.

In 1979-85 worked on developing vectors and methods of plant transformation with Agrobacterium. First in the USSR performed plant transformation that was recognized with issued USSR patents.

In 1985-95 in collaboration with others first in Russia performed transformation of several genes into plants including genes having potential practical application like insect resistance (BT gene), herbicide resistance, cytokinin biosynthesis gene, glucose isomerase gene. Developed an experimental model to study phytohormonal regulation in plants using expression of bacterial genes.

Current research interest is in making edible vaccines and pharmaceuticals in plants. Particularly development of vaccines against such diseases as anthrax, smallpox, and SARS is in progress in my lab under joint BFL Program. The approach is to use the expression in transgenic plants of a protein from pathogen, which is commonly used for traditional vaccination. The advantage of PA production in plants includes lower risk and purification cost comparing with vaccine production from attenuated pathogen. Making a plant-derived vaccine is tightly connected with a common problem of the expression of foreign proteins in plants that is important for production of cost-effective vaccines. Approaches to get higher accumulation of a transgene protein in plants use the advantage of fusion of target protein with other proteins, which are known to accumulate at high level, and targeting of vaccine proteins to cell compartments. Another approach to increase accumulation of ‘badly expressed’ vaccine proteins in transgenic plants is to use co-expression of chaperone proteins that could stabilize and solubilize other proteins accumulated in the same plant.

Other Expertise

University education and initial research experience received in experimental Radiation Physics.

Additionally to plant molecular biology experienced to work with different bacteria, plant viruses, and yeast. Particularly, had experience to work on plant genomics and yeast two-hybrid system.

In 1995-98, working with Prof. S. Howell at BTI, was mostly involved in a genomic project on cloning of an Arabidopsis gene (Car1), which confers resistance to systemic infection with cauliflower mosaic virus(CaMV) (see: http://bti.cornell.edu/pcbg/HowellLab/Plant-virus%20interact%20page). A possible involvement in virus resistance of plant defense response was studied (MPMI 1996, 9, 810). Then fine chromosome mapping of the gene was performed that permittedfurther cloning using chromosome walking strategy. But recent success in sequencing of the Arabidopsis genome suggested that these mapping data should be combined with the arising genomic data for easier cloning. A second project was also devoted to a study of CaMV movement in Arabidopsis. Yeast two-hybrid system was used to identify plant proteins that interact with CaMV movement protein (MP). Screening of an Arabidopsis cDNA library for proteins that interact with MP revealed at least three candidates that interact with N-terminal third of the CaMV MP. One clone MPIP7 is similar to a rat protein (PRA1) that associates with the vesicle transport machinery in mammalian cells and is described as a dual prenylated rab and VAMP2 receptor. Further study in the same lab revealed that some non-infectious CaMV MP mutants failed to interact with MPIP7 while the MP from infectious second-site mutant interacted positively. This is consistent with a presumed role of the CaMV MP in viral movement and suggests that the viral MP may interact with a component of the vesicle trafficking machinery in plants.

In 1998-2000 was involved in transformation of Gladiolus plants to obtain resistance for cucumber mosaic virus (CMV). Expression of a defective CMV replicase and/or coat protein (CP) gene(s) was used to generate virus resistance in Gladiolus. Several independently transformed transgenic lines were selected and analyzed for each gene combination. Northern and immuno analysis were used to study the expression of transgenes.

Review Panels and Committees:
June 2001 Center for Scientific Review Special Emphasis Panel, Council ZRG1 SSS-2, National Institutes of Health, Bethesda, MD.

2000-01 - Cooperative Grant Program of the U.S. Civilian Research and Development Foundation (CRDF) for the Independent States of the Former Soviet Union Review Panels, Arlington VA.

January 2000 - United States-Israel Bi-National Agricultural Research and Development Fund. BARD Review Panel, P.O. Box 6, Bet-Dagan 50250, Israel.

Nov. 1998 - Expanding Agricultural Production Review Panel. The Middle East Regional Cooperation Program (MERC) and U.S.-Israel Cooperative Development Research (CDR) Program, U.S. Agency for International Development. Washington DC, USA.

1990-1995 - The former USSR and Russian State Research Programs in Plant Biotechnology and Genetic Engineering Review Panels.

1986-1988 - Chairman of the 'Youth Summer School on Molecular Genetics' organized by the Institute of Molecular Genetics (held in Uzhgorod, Ukraine, 1986, 1987 and Dushanbe, Tajikistan, 1988).

Future Research

Many recent immunological studies reveals that some bacterial toxins can stimulate immune responses when fused with other peptid antigens. This is of great practical importance because permits to obtain more effective vaccines to many otherwise poorly immunogenic antigens. Our future research aimed to make multiple vaccines in plants using stimulatory effect of protein fusions.

Industrial Relevance

Although many aspects of our study are theoretical and intended to develop common approach for generation edible vaccines in plants, eventually the developed methods and transgenic plants may be valuable for pharmaceutical industry.

Keywords

COS Keywords:

Genetic Engineering, Microbiology, Molecular Biology, Molecular Genetics, Plant Genome, Plant Sciences, Plant Virology, Transgenic Plants, Transgenics.

Additional Terms:

Plant Edible Vaccines.

Languages

(Reading, Writing, Speaking)

Russian: (Fluent, Fluent, Fluent)

Memberships

All-Russian Society of Geneticists and Breeders

Honors and Awards

1993, Doctor of Sciences Degree in Biology, All-Russian Supreme Attestation Committee, Institute of General Genetics, Russian Academy of Sciences, For Achievements in Plant Molecular Biology

Previous Positions

1998-2000, Research Molecular Biologist, USDA/ARS, U.S. National Arboretum, Floral Nursery Plant Research Unit
1995-1998, Visiting Researcher, Cornell University, Boyce Thompson Institute for Plant Research, Howell Laboratory
1986-1989, Senior Scientist, Institute of Molecular Genetics, Plant Molecular Genetics
1979-1985, Post-Doctoral Research Scientist, Institute of Molecular Genetics, Laboratory of Plant Molecular Genetics
1974-1979, Research Scientist, N.I.Vavilov Institute of General Genetics, Laboratory of Biochemical Genetics
1973-1974, Engineer, USSR State Department of Health, Institute for Biomedical Problems, Radiation Physics

Patents

The method of transgenic plant regeneration, recombinant vector plasmid pTiVY4 DNA for gene transfer into plants and Agrobacterium tumefaciens bacterial strain for plant transformation., Patent Number: SU 1547316 A1, 1989, Institution-owned, Russia.
The method of gene transfer into plants, Agrobacterium tumefaciens bacterial strain and recombinant vector plasmid pAK320Neo DNA for gene transfer into plants., Patent Number: SU 1427833 A1, 1988, Institution-owned, Russia.
Vector plasmid pAK333 for DNA fragments cloning in Escherichia coli and Rhizobiaceae bacterial cells, the method of its construction and Escherichia coli bacterial strain for pAK333 vector production., Patent Number: SU 1389299 A1, 1987, Institution-owned, Russia.

Funding Received

  • ISTC (International Science @ Technology Center) research project # T-1105: Genome analysis of cereal crops in Tajikistan, 2004 to 2007.
  • International Science Foundation: Travel Grant for Outstanding Scientists, 1994 to 1994.
  • International Science Foundation: Support Grant for Outstanding Scientists in the former USSR, 1993 to 1993.
  • Russian State Scientific Programs: 'Priority Directions in Genetics' and 'Modern Methods in Bioengineering': Co-applicator of Research Projects, 1991 to 1994.

Publications

  • Golovkin M., Spitsin S., Andrianov V., Smirnov Yu., Xiao Y., Pogrebnyak N., Markley K., Brodzik R., Gleba Yu., Isaacs S.N., and Koprowski H. (2007) ·Smallpox subunit vaccine produced in planta confers protection in mice., Proc. Natl. Acad. Sci.,, 104, 6864-6869
  • Pogrebnyak N., Golovkin M., Andrianov V., Spitsin S., Smirnov Yu., Egolf R., Koprowski H., Severe Acute Respiratory Syndrome (SARS) S Protein Production in Plants: Development of Recombinant Vaccine, Proc. Natl. Acad. Sci., 102(25), 9062-9067, June 21, 2005
  • Pogrebnyak N., Andrianov V., Spitsin S., Towards the Development of Edible Vaccine Against SARS Coronavirus. In: Molecular Approaches to Vaccine Design, Abstracts, Cold Spring Harbor, NY, Cold Spring Harbor Laboratories, p. 32, 4-7 Dec 2003
  • Huang Zh., Andrianov V.M., Han Y., Howel, Identification of Arabidopsis Proteins That Interact With the Cauliflower Mosaic Virus (CaMV) Movement Protein, Plant Molecular Biology, 47, 663-675, 2001
  • Makarova R.V., Andrianov V.M., Borisova T.A., Piruzyan E.S., Kefeli V.I., Morphogenetic Manifestations of the Expression of the Bacterial ipt Gene in Regenerated Tobacco Plants in vitro, Fiziologiya Rastenii (Russian Plant Physiology), 44(1), 11-19, 1997
  • Makarova R.V., Borisova T.A., Vlasov P.V., Machᣫová ‰., Andrianov V.M., Piruzyan E.S., Kefeli V.I., Phytohormone Production By Tobacco Ipt-Regenerants in Vitro, Fiziologiya Rastenii (Russian Plant Physiology), 44(5), 762-768, 1997
  • Callaway A, Liu W, Andrianov V, Stenzler L, Zhao J, Wettlaufer S, Jayakumar P, Howell SH, Characterization of cauliflower mosaic virus (CaMV) resistance in virus-resistant ecotypes of Arabidopsis, Molecular Plant-microbe Interactions, 9(9), 810-8, December 1996 Abstract
  • Andrianov V., Kononova, M., Myasoedov N., Piruzian E., Bacterial isopentenyl transferase gene expression input into phytohormonal balance alteration in different parts of transgenic plants, Andrianov V., Kononova, M., Myasoedov N., Piruzian E. Bacterial isopentenyl transferase gene expression input into phytoh In: 4th International Congress of Plant Molecular Biology, Amsterdam, Abstract #928, 1994
  • Krasheninnikova LV, Rassadina GV, Kirsanova SN, Khromova LM, Iusibov VM, Pak Chun Ir, Andrianov VM, Piruzian ES, Obtaining transgenic Solanum tuberosum potato plants with active bacterial genes xyl and T-cyt effecting the phytohormone balance, Molekuliarnaia Genetika, Mikrobiologia, I Virusologa, 17-20, November 1991 Abstract
  • Yusibov VM, Il PC, Andrianov VM, Piruzian ES, Phenotypically normal transgenic T-cyt tobacco plants as a model for the investigation of plant gene expression in response to phytohormonal stress, Plant Molecular Biology, 17(4), 825-36, October 1991 Abstract
  • Norova G.Ye., Kaliev A.B., Andrianov V.M., Piruzyan E.S., Transformation of tomato Licopersicon esculentun L. cv. 'Bakhtemir' plants, Biotekhnologiya (Russ.), 16-18, 1991
  • Ulmasov TN, Gulov MK, Aliev KA, Andrianov VM, Piruzian ES, Nucleotide sequences of the chloroplast psbA and trnH genes from cotton Gossypium hirsutum, Nucleic Acids Research, 18(1), 186, January 1990 Abstract
  • Gulov MK, Ulmasov TN, Aliev KA, Andrianov VM, Piruzian ES, Nucleotide sequence of the chloroplast rbcL gene from cotton Gossypium hirsutum, Nucleic Acids Research, 18(1), 185, January 1990 Abstract
  • Lee K.G., Andrianov V.M., Piruzian E.S., Agrobacterium tumefaciens nopaline-type Ti-megaplasmid C58 virD locus is positively regulated, Izvestiya Akademii Nauk SSSR, Seriya Biologicheskaya (Russ.), 325-328, 1990
  • Evstratova V.V., Ulmasov T.N., Andrianov V.M., Piruzian E.S., Introduction of delta-endotoxin gene yielding resistance to Lepidoptera from Bacillus thuringiensis subspecies kurstaki HD-1-Dipel into Nicotiana tabacum plants, Biotekhnologiya (Russ.), 26-29, 1990
  • Ul'masov TN, Gulov MK, Aliev KA, Andrianov VM, Piruzian ES, Cloning of chloroplast psbA and rbcL-genes from cotton Gossipium hirsutum, Molekuliarnaia Genetika, Mikrobiologia, I Virusologa, 17-20, August 1989 Abstract
  • Iusibov VM, Pogosian GP, Andrianov VM, Piruzian ES, Transfer of the agrobacterial gene for cytokinin biosynthesis into tobacco plants, Molekuliarnaia Genetika, Mikrobiologia, I Virusologa, 11-3, July 1989 Abstract
  • Li KG, Kobets NS, Andrianov VM, Piruzian, The study of binding of the virG gene product with the virD gene promotor region of the Agrobacterium tumefaciens Ti-plasmid C58, Molekuliarnaia Genetika, Mikrobiologia, I Virusologa, 6-11, June 1989 Abstract
  • Andrianov V.M., Molecular and genetic basis of plant tumor induction by agrobacteria, MolecuIn: Advances in Science and Technique. Ser. General Genetics. v. 11. Molecular-Genetic Mechanisms of Plant Cell Proliferation. M, VINITI ed. (Russ.), 82-205, 1989
  • Argentova V.V., Andrianov V.M., Piruzian E.S., Cloning of bacterial plasmids' sequences from Nicotiana debneyi plant cells transformed by microinjection of DNA, Genetika (Russian), XXV(5), 799-808, 1989
  • Srivastava D.K., Andrianov V.M., Piruzian E.S., Tissue culture and plant regeneration of watermelon (Citrullus vulgaris Schrad var Melitopolski), Plant Cell Reports, 8, 300-302, 1989
  • Shestakov S.V., Yelanskaya I.V., Yermakova S.Yu., Andrianov V.M., Ulmasov T.N., Koksharova O.A., Cloning of the DNA fragments which restore photosynthetic ability in mutants of Synechocystis 6803, Doklady Akademii Nauk SSSR (Russ.), 308(1), 211-214, 1989
  • Piruzian ES, Andrianov VM, Iusibov VM, Mett VL, Expression of the Escherichia coli glucose isomerase gene in transgenic plants, Doklady Akademii Nauk Sssr, 305(3), 729-31, 1989 Abstract
  • Andrianov V.M., Phytohormonal balance alteration after tumorous transformation of plant cells by Agrobacterium tumefaciens Ti plasmids, In: 'Growth and Resistance of Plants', 'Nauka', Novosibirsk (Russ.), 22-29, 1988
  • Srivastava D.K., Andrianov V.M., Piruzian E.S., Organogenesis and plant regeneration in water-melon tissue culture, Fiziologiya Rastenii (Plant Physiol. Russ.), 35(6), 1243-1247, 1988
  • Evstratova V.V., Kaliev A.B., Andrianov V.M., Piruzian E.S., The use of the vector Agrobacterium plasmids for transferring of delta-endotoxine gene from Bacillus thuringiensis into plants, Vestnik Selsko-Khozyaistvennoi Nauki (Russ.: Trends in Agricultural Science), 8(384), 71-77, 1988
  • Argentova V.V., Pasternak T.P., Ulmasov T.N., Melnikov P.V., Andrianov V.M., Gleba Yu.Yu., Piruzian E.S., Argentova V.V., Pasternak T.P., Ulmasov T.N.ce of Agrobacterium Ti plasmStudies on the influenrs on integration into the plant cell genome by microinjection, Genetika (Russian), XXIV(10), 1730-1738, 1988
  • Kozarov E., Kaliev A., Andrianov V., Piruzian E., Construction of plant expression vector active in prokaryotic cells, Doklady Bolgarskoi Academii Nauk, 41(9), 117-118, 1988
  • Stekhin IN, Andrianov VM, Piruzian ES, Conjugation transfer of the bireplicon plasmid pSPA044 into Rhizobiaceae bacteria, Molekuliarnaia Genetika, Mikrobiologia, I Virusologa, 31-3, October 1986 Abstract
  • Andrianov V.M., Perspectives for application of Ti plasmids in plant gene engineering, Selskohkozyaistvennaya Biologia (Russ.), 48-56, 1986
  • Pasternak T.P., Melnikov P.V., Gleba Yu.Yu., Sytnik K.M., Argentova V.V., Kaliev A.B., Andrianov V.M., Piruzian E.S., Pasternak T.P., Melnikov P.V., Gleba Yu.Yu., Sytnik K.M., ArgentovaGenetic transformation of higher plant cells by DNA microinjections, Izvestiya Akademii Nauk SSSR, Seriya Biologicheskaya (Russ.), 314-316, 1986
  • Stekhin I.N.., Andrianov V.M., Piruzian E.S., Stekhin I.N.., Bireplicon plasmid pSPA044 transfer by conjugation into bacteria of Rhizobiaceae family, Molekulyarnaya genetika, microbiologia and virusologia (Russ.), 31-33, 1986
  • Piruzian E.S., Andrianov V.M., Agrobacterium Plasmids and Plant Genetic Engineering, Monograph. Nauka, Moscow, 1-280, 1985
  • Chernin L.S., Avdienko I.D., Shchipkova N.I., Ovadis M.I., Andrianov V.M., Chernin L.Two regions of pTiC58 plasmid determine auxin production in Agrobacterium tumefaciens, Genetika (Russian), XXI(9), 1428-1437, 1985
  • Andrianov V.M., Agrobacterium plasmids as potential vector systems for genetic engineering in higher plants, Genetika (Russian), XX(5), 709-721, 1984
  • Stekhin I.N., Andrianov V.M., Piruzian E.S., Stekhin I.N., AnConstruction of hybrid pland Agrobacterium tumefaciens cells on the basis of pBR325 and nopaline type asmids capable to replicate in Escherichia coli Ti plasmid vectors, Molekulyarnaya genetika, microbiologia and virusologia (Russ.), 19-23, 1984
  • Mogutov MA, Kobets NS, Velikodvorskaia GA, Andrianov VM, Piruzian ES, Integration of the mini-Mu phage into multicopy plasmids, Genetika, 20(1), 16-25, January 1984 Abstract
  • Piruzian ES, Stekhin IN, Andrianov VM, Cloning of the Ti-plasmid DNA fragments of Agrobacterium tumefaciens in Escherichia coli and the identification of the site of origin of Ti-plasmid replication, Doklady Akademii Nauk Sssr, 273(5), 1249-51, 1983 Abstract
  • Piruzian E, Andrianov V, Mogutov M, Krivtsova E, Yuzeeva V, Vetoshkin A, Kobets N, Specificity of bacteriophage Mu integration into DNAs of different origins, Cold Spring Harbor Symposia On Quantitative Biology, 45 Pt 1, 365-9, 1981 Abstract
  • Andrianov VM, Amerkhanova MB, Vinetskii IuP, Chloroplast DNA cloning in Escherichia coli. II. The properties of the recombinant plasmids bearing the EcoRI fragments of pea chloroplast DNA and the cloning of the DNA sequences with rRNA genes, Genetika, 15(11), 1918-24, November 1979 Abstract
  • Poluektova E.U., Andrianov V.M., Vinetski Yu.P., Prozorov A.A., Poluektova E.U., Andrianov V.M., Vinetski Yu.P.Some properties of the defective phage isolated from the rec149 Bacillus subtilis mutant cells and its DNA, Genetika (Russian), XV(7), 1199-1205, 1979
  • Andrianov VM, Zemlianaia NIu, Karimov M, Amerkhanova MB, Vinetskii IuTs, Cloning chloroplast DNA in escherichia coli. I. Construction and selection of recombinant plasmids containing fragments of pea chloroplast DNA, Genetika, 14(9), 1503-12, September 1978 Abstract
  • Andrianov V.M., Fedotov A.R., Shiyan A.N., Amerkhanova M.B., Vinetski Yu.P., Andrianov V.M., Fedotov A.R.Selection of colicine-defective recombinant plasmids by mitomycin C, Gene, 2(5-6), 317-322, 1977
  • Kislina O.S., Naroditski B.S., Chapligina N.M., Karamov E.B., Tikhonenko T.I., Dreisin R.S., Zolotarskaya E.V., Andrianov V.M., Vinetski Yu.P., Digestion and splicing of monkey type 7 adenovirus DNA by EcoRI restriction endonuclease and DNA-ligase, Voprosy Virusologii (Russ.), 271-275, 1977
  • Andrianov VM, Fedotov AR, Shiian AN, Amerkhanova MB, Vinetskii IuP, Selection of recombinant plasmids using mitomycin C, Doklady Akademii Nauk Sssr, 231(5), 1237-40, 1976 Abstract
  • Vinetski Yu.P., Andrianov V.M., Alexandrova N.M., Kuz'mina Ye.A., A genetic method of assay of transitions and transversions in the rIIA region of the phage T4B, Genetika, X(3), 102-110, 1974
  • Vinetski Yu.P., Andrianov V.M., Ivanov V.I., Marker-specificity of the high negative interference in phage T4 recombination, Genetika, X(4), 120-128, 1974

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