Rudolf H. Aebersold

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University of Washington
School of Medicine
Molecular Biotechnology
Associate ProfessorAppointed: 1993

Mailing Address

Box 357730
University of Washington
Seattle, Washington 98195
United States

Contact Information

Phone: (206) 685-4235
Fax: (206) 685-6932
ruedi@u.washington.edu

Qualifications

Ph.D., Switzerland-Med Fac University of Basel, 1983.

Expertise and Research Interests

The objective of research in our group is the development of new technologies for the comprehensive analysis of regulated biological systems and to apply these technologies to the study of the signal transduction pathways which induce and control the fate of T cells during development and activation.

These signal transduction pathways, like other regulated systems, depend on the coordinated expression and activation of multiple genes and their respective products. We are developing new experimental approaches for the identification of the proteins which are part of a particular pathway and for the determination of the function and the state of activity of the identified proteins.

Our approach is based on the integration of high resolution proteinsand peptide separation techniques with high sensitivity detectors such as mass spectrometers and atomic force microscopes.

As the number of the gene sequences determined is rapidly increasing, we anticipate that technologies which focus on the characterization of proteins, i.e., the biological effector molecules and their state of activity will become essential tools for the comprehensive analysis of biological systems.

Other Expertise

Consultant:
MesaGnostics, La Jolla, 1994-present;
Abiotic Systems, Palo Alto., 1994-present;
Millipore Corp., 1988-1993.

Editorial Board:
Protein Science, 1992-present;
Analytical Biochemistry, 1991-present;
Electrophoresis, 1989-1993.

Manuscript Reviewer:
Grant Review Study Section H, 1990-1992;
Protein Science;
Analytical Biochemistry;
Journal of Biological Chemistry;
TEBS;
National Cancer Institute of Canada.

Advisory Committees:
Priorities and Planning Committee, Canadian Genetic Diseases Network, 1989-1993;
NIH Mass Spectrometry Resource Center at Rockefeller University;
Resource Advisory Committee, QUEST Protein Database Center, Cold Spring Harbor Laboratory.

Research contract with Pfizer Inc., on structural and functional studies of non-receptor protein tyrosine kinases

NIH Special Study Section, 1992

Keywords

COS Keywords:

Biochemistry, Proteins, Bioengineering, Biotechnology, Cell Biology, Chemical Sciences, Mass Spectrometry, Microbiology, Molecular Genetics, Peptides, Proteins and Macromolecules.

Additional Terms:

Bioengineering, Cell Biology, Microbiology, Molecular Engineering, Specific Cell Types, Viral Disease, Viruses.

Memberships

American Association for the Advancement of Science
American Society for Biochemistry and Molecular Biology
Biotechnology Alliance of British Columbia
New York Academy of Sciences (NYAS)
Protein Society

Previous Positions

1989-1993, Assistant Professor, University of British Columbia
1987-1988, California Institute of Technology, Biology, Senior Research Fellow
1984-1986, California Institute of Technology, Biology, Postdoctoral Position\

Publications

  • Ostrowski J, Schullery DS, Denisenko ON, Higaki Y, Watts J, Aebersold R, Stempka L, Gschwendt M, Bomsztyk K, Role of tyrosine phosphorylation in the regulation of the interaction of heterogenous nuclear ribonucleoprotein K protein with its protein and RNA partners., Journal of Biological Chemistry, 275(5), 3619-28, 4 2000 Abstract
  • Goodlett DR, Aebersold R, Watts JD, Quantitative in vitro kinase reaction as a guide for phosphoprotein analysis by mass spectrometry., Rapid Communications in Mass Spectrometry, 14(5), 344-8, 2000 Abstract
  • Figeys D, Corthals GL, Gallis B, Goodlett DR, Ducret A, Corson MA, Aebersold R, Data-dependent modulation of solid-phase extraction capillary electrophoresis for the analysis of complex peptide and phosphopeptide mixtures by tandem mass spectrometry: application to endothelial ni, Anal Chem, 71(13), 2279-87, 1 Jul 1999 Abstract
  • Watts JD, Gu M, Patterson SD, Aebersold R, Polverino AJ, On the complexities of ceramide changes in cells undergoing apoptosis: lack of evidence for a second messenger function in apoptotic induction., Cell Death and Differentiation, 6(2), 105-14, February 1999 Abstract
  • Ducret A, Bartone N, Haynes PA, Blanchard A, Aebersold R, A simplified gradient solvent delivery system for capillary liquid chromatography-electrospray ionization mass spectrometry., Anal Biochem, 265(1), 129-38, 1 Dec 1998 Abstract
  • Figeys D, Aebersold R, Nanoflow solvent gradient delivery from a microfabricated device for protein identifications by electrospray ionization mass spectrometry., Anal Chem, 70(18), 3721-7, 15 Sep 1998 Abstract
  • Haynes PA, Gygi SP, Figeys D, Aebersold R, Proteome analysis: biological assay or data archive?, Electrophoresis, 19(11), 1862-71, August 1998 Abstract
  • Figeys D, Aebersold R, High sensitivity analysis of proteins and peptides by capillary electrophoresis-tandem mass spectrometry: recent developments in technology and applications., Electrophoresis, 19(6), 885-92, May 1998 Abstract
  • Steinmetz A, Hermann M, Nimpf J, Aebersold R, Ducret A, Weinberg R B, Schneider W J, Expression and conservation of apolipoprotein AIV in an avian species., Journal of Biological Chemistry, 273(17), 10543-9, 24 Apr 1998 Abstract
  • Ducret A, Van Oostveen I, Eng J K, Yates J R 3rd, Aebersold R, High throughput protein characterization by automated reverse-phase chromatography/electrospray tandem mass spectrometry., Protein Science, 7(3), 706-19, March 1998 Abstract
  • Figeys D, Ning Y, Aebersold R, A microfabricated device for rapid protein identification by microelectrospray ion trap mass spectrometry., Analytical Chemistry, 69(16), 3153-60, 15 Aug 1997 Abstract
  • Figeys D, Aebersold R, Capillary electrophoresis of peptides and proteins at neutral pH in capillaries covalently coated with polyethyleneimine., Journal of chromatography. B, Biomedical Sciences and Applications, 695(1), 163-8, 18 Jul 1997 Abstract
  • Watts J D, Gu M, Polverino A J, Patterson S D, Aebersold R, Fas-induced apoptosis of T cells occurs independently of ceramide generation., Proceedings of the National Academy of Sciences (USA), 94(14), 7292-6, 8 Jul 1997 Abstract
  • Ducret A, Bures E J, Aebersold R, High-sensitivity detection of 4-(3-pyridinylmethylaminocarboxypropyl) phenylthiohydantoins by capillary liquid chromatography-microelectrospray ion trap mass spectrometry., Journal of Protein Chemistry, 16(5), 323-8, July 1997 Abstract
  • Takaki S, Watts J D, Forbush K A, Nguyen N T, Hayashi J, Alberola-Ila J, Aebersold R, Perlmutter R M, Characterization of Lnk. An adaptor protein expressed in lymphocytes., Journal of Biological Chemistry, 272(23), 14562-70, 6 Jun 1997 Abstract
  • van Oostveen I, Ducret A, Aebersold R, Colloidal silver staining of electroblotted proteins for high sensitivity peptide mapping by liquid chromatography-electrospray ionization tandem mass spectrometry., Analytical Biochemistry, 247(2), 310-8, 1 May 1997 Abstract
  • Palaty C K, Kalmar G, Tai G, Oh S, Amankawa L, Affolter M, Aebersold R, Pelech S L, Identification of the autophosphorylation sites of the Xenopus laevis Pim-1 proto-oncogene-encoded protein kinase., Journal of Biological Chemistry, 272(16), 10514-21, 18 Apr 1997 Abstract
  • Morris N J, Ducret A, Aebersold R, Ross S A, Keller S R, Lienhard G E, Membrane amine oxidase cloning and identification as a major protein in the adipocyte plasma membrane., Journal of Biological Chemistry, 272(14), 9388-92, 4 Apr 1997 Abstract
  • Johnson J E, Aebersold R, Cornell R B, An amphipathic alpha-helix is the principle membrane-embedded region of CTP:phosphocholine cytidylyltransferase. Identification of the 3-(trifluoromethyl)-3-(m-[125I]iodophenyl) diazirine photolabeled, Biochimica Et Biophysica Acta, 1324(2), 273-84, 13 Mar 1997 Abstract
  • Figeys D, Aebersold R, High sensitivity identification of proteins by electrospray ionization tandem mass spectrometry: initial comparison between an ion trap mass spectrometer and a triple quadrupole mass spectrometer., Electrophoresis, 18(3-4), 360-8, March 1997 Abstract
  • Figeys D, Ducret A, Aebersold R, Identification of proteins by capillary electrophoresis-tandem mass spectrometry. Evaluation of an on-line solid-phase extraction device., Journal of chromatography. A, 763(1-2), 295-306, 28 Feb 1997 Abstract
  • Gu M, Kerwin J L, Watts J D, Aebersold R, Ceramide profiling of complex lipid mixtures by electrospray ionization mass spectrometry., Analytical Biochemistry, 244(2), 347-56, 15 Jan 1997 Abstract
  • Raval-Fernandes S, Sawant L A, Aebersold R H, Ducret A, Rome L H, Axonal proteins involved in myelination: characterization of a collagen-like protein., Developmental Neuroscience, 19(5), 421-9, 1997 Abstract
  • La Roche J, van der Staay G W, Partensky F, Ducret A, Aebersold R, Li R, Golden S S, Hiller R G, Wrench P M, Larkum A W, Green B R, Independent evolution of the prochlorophyte and green plant chlorophyll a/b light-harvesting proteins., Proceedings of the National Academy of Sciences (USA), 93(26), 15244-8, 24 Dec 1996 Abstract
  • Durnford D G, Aebersold R, Green B R, The fucoxanthin-chlorophyll proteins from a chromophyte alga are part of a large multigene family: structural and evolutionary relationships to other light harvesting antennae., Molecular and General Genetics, 253(3), 377-86, 13 Dec 1996 Abstract
  • Watts J D, Brabb T, Bures E J, Wange R L, Samelson L E, Aebersold R, Identification and characterization of a substrate specific for the T cell protein tyrosine kinase ZAP-70., Febs Letters, 398(2-3), 217-22, 2 Dec 1996 Abstract
  • Figeys D, Ducret A, Yates J R 3rd, Aebersold R, Protein identification by solid phase microextraction-capillary zone electrophoresis-microelectrospray-tandem mass spectrometry [see comments], Nature Biotechnology, 14(11), 1579-83, November 1996 Abstract
  • Isakov N, Wange R L, Watts J D, Aebersold R, Samelson L E, Purification and characterization of human ZAP-70 protein-tyrosine kinase from a baculovirus expression system., Journal of Biological Chemistry, 271(26), 15753-61, 28 Jun 1996 Abstract
  • Figeys D, van Oostveen I, Ducret A, Aebersold R, Protein identification by capillary zone electrophoresis/microelectrospray ionization-tandem mass spectrometry at the subfemtomole level., Analytical Chemistry, 68(11), 1822-8, 1 Jun 1996 Abstract
  • Ducret A, Bruun C F, Bures E J, Marhaug G, Husby G, Aebersold R, Characterization of human serum amyloid A protein isoforms separated by two-dimensional electrophoresis by liquid chromatography/electrospray ionization tandem mass spectrometry., Electrophoresis, 17(5), 866-76, May 1996 Abstract
  • Tull D, Burgoyne D L, Chow D T, Withers S G, Aebersold R, A mass spectrometry-based approach for probing enzyme active sites: identification of Glu 127 in Cellulomonas fimi exoglycanase as the residue modified by N-bromoacetyl cellobiosylamine., Analytical Biochemistry, 234(2), 119-25, 15 Feb 1996 Abstract
  • Ng D H, Watts J D, Aebersold R, Johnson P, Demonstration of a direct interaction between p56lck and the cytoplasmic domain of CD45 in vitro., Journal of Biological Chemistry, 271(3), 1295-300, 19 Jan 1996 Abstract
  • Gebler J C, Trimbur D E, Warren A J, Aebersold R, Namchuk M, Withers S G, Substrate-induced inactivation of a crippled beta-glucosidase mutant: identification of the labeled amino acid and mutagenic analysis of its role., Biochemistry, 34(44), 14547-53, 7 Nov 1995 Abstract
  • Keller S R, Scott H M, Mastick C C, Aebersold R, Lienhard G E, Cloning and characterization of a novel insulin-regulated membrane aminopeptidase from Glut4 vesicles [published erratum appears in J Biol Chem 1995 Dec 15;270(50):30236], Journal of Biological Chemistry, 270(40), 23612-8, 6 Oct 1995 Abstract
  • Patterson S D, Aebersold R, Mass spectrometric approaches for the identification of gel-separated proteins., Electrophoresis, 16(10), 1791-814, October 1995 Abstract
  • Wange R L, Guitian R, Isakov N, Watts J D, Aebersold R, Samelson L E, Activating and inhibitory mutations in adjacent tyrosines in the kinase domain of ZAP-70., Journal of Biological Chemistry, 270(32), 18730-3, 11 Aug 1995 Abstract
  • Dechert U, Affolter M, Harder K W, Matthews J, Owen P, Clark-Lewis I, Thomas M L, Aebersold R, Jirik F R, Comparison of the specificity of bacterially expressed cytoplasmic protein-tyrosine phosphatases SHP and SH-PTP2 towards synthetic phosphopeptide substrates., European Journal of Biochemistry, 231(3), 673-81, 1 Aug 1995 Abstract
  • Withers S G, Aebersold R, Approaches to labeling and identification of active site residues in glycosidases., Protein Science, 4(3), 361-72, March 1995 Abstract
  • Tull D, Miao S, Withers S G, Aebersold R, Identification of derivatized peptides without radiolabels: tandem mass spectrometric localization of the tagged active-site nucleophiles of two cellulases and a beta-glucosidase., Analytical Biochemistry, 224(2), 509-14, 20 Jan 1995 Abstract
  • Agrez M V, Meldrum C J, Sim A T, Aebersold R H, Clark I M, Cawston T E, Burns G F, A fibroblast elongation factor purified from colon carcinoma cells shares sequence identity with TIMP-1., Biochemical and Biophysical Research Communications, 206(2), 590-600, 17 Jan 1995 Abstract
  • Wange R L, Isakov N, Burke T R Jr, Otaka A, Roller P P, Watts J D, Aebersold R, Samelson L E, F2(Pmp)2-TAM zeta 3, a novel competitive inhibitor of the binding of ZAP-70 to the T cell antigen receptor, blocks early T cell signaling., Journal of Biological Chemistry, 270(2), 944-8, 13 Jan 1995 Abstract
  • Isakov N, Wange R L, Burgess W H, Watts J D, Aebersold R, Samelson L E, ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity., Journal of Experimental Medicine, 181(1), 375-80, 1 Jan 1995 Abstract
  • Hess D, Nika H, Chow D T, Bures E J, Morrison H D, Aebersold R, Liquid chromatography-electrospray ionization mass spectrometry of 4-(3-pyridinylmethylaminocarboxypropyl) phenylthiohydantoins., Analytical Biochemistry, 224(1), 373-81, 1 Jan 1995 Abstract
  • Bures E J, Nika H, Chow D T, Morrison H D, Hess D, Aebersold R, Synthesis of the protein-sequencing reagent 4-(3-pyridinylmethylaminocarboxypropyl) phenyl isothiocyanate and characterization of 4-(3-pyridinylmethylaminocarboxypropyl) phenylthiohydantoins., Analytical Biochemistry, 224(1), 364-72, 1 Jan 1995 Abstract
  • Amankwa L N, Harder K, Jirik F, Aebersold R, High-sensitivity determination of tyrosine-phosphorylated peptides by on-line enzyme reactor and electrospray ionization mass spectrometry., Protein Science, 4(1), 113-25, January 1995 Abstract
  • Watts J D, Affolter M, Krebs D L, Wange R L, Samelson L E, Aebersold R, Identification by electrospray ionization mass spectrometry of the sites of tyrosine phosphorylation induced in activated Jurkat T cells on the protein tyrosine kinase ZAP-70., Journal of Biological Chemistry, 269(47), 29520-9, 25 Nov 1994 Abstract
  • Affolter M, Watts J D, Krebs D L, Aebersold R, Evaluation of two-dimensional phosphopeptide maps by electrospray ionization mass spectrometry of recovered peptides., Analytical Biochemistry, 223(1), 74-81, 15 Nov 1994 Abstract
  • Gold M R, Chiu R, Ingham R J, Saxton T M, van Oostveen I, Watts J D, Affolter M, Aebersold R, Activation and serine phosphorylation of the p56lck protein tyrosine kinase in response to antigen receptor cross-linking in B lymphocytes., Journal of Immunology, 153(6), 2369-80, 15 Sep 1994 Abstract
  • Saxton T M, van Oostveen I, Bowtell D, Aebersold R, Gold M R, B cell antigen receptor cross-linking induces phosphorylation of the p21ras oncoprotein activators SHC and mSOS1 as well as assembly of complexes containing SHC, GRB-2, mSOS1, and a 145-kDa tyrosine-p, Journal of Immunology, 153(2), 623-36, 15 Jul 1994 Abstract
  • Deretic D, Aebersold R H, Morrison H D, Papermaster D S, Alpha A- and alpha B-crystallin in the retina. Association with the post-Golgi compartment of frog retinal photoreceptors., Journal of Biological Chemistry, 269(24), 16853-61, 17 Jun 1994 Abstract
  • Miao S, Ziser L, Aebersold R, Withers S G, Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry., Biochemistry, 33(23), 7027-32, 14 Jun 1994 Abstract
  • Winz R, Hess D, Aebersold R, Brownsey R W, Unique structural features and differential phosphorylation of the 280-kDa component (isozyme) of rat liver acetyl-CoA carboxylase., Journal of Biological Chemistry, 269(20), 14438-45, 20 May 1994 Abstract
  • Miao S, McCarter J D, Grace M E, Grabowski G A, Aebersold R, Withers S G, Identification of Glu340 as the active-site nucleophile in human glucocerebrosidase by use of electrospray tandem mass spectrometry., Journal of Biological Chemistry, 269(15), 10975-8, 15 Apr 1994 Abstract
  • Zhang W, Czernik A J, Yungwirth T, Aebersold R, Chait B T, Matrix-assisted laser desorption mass spectrometric peptide mapping of proteins separated by two-dimensional gel electrophoresis: determination of phosphorylation in synapsin I., Protein Science, 3(4), 677-86, April 1994 Abstract
  • Gold M R, Yungwirth T, Sutherland C L, Ingham R J, Vianzon D, Chiu R, van Oostveen I, Morrison H D, Aebersold R, Purification and identification of tyrosine-phosphorylated proteins from B lymphocytes stimulated through the antigen receptor., Electrophoresis, 15(3-4), 441-53, March 1994 Abstract
  • Harder K W, Owen P, Wong L K, Aebersold R, Clark-Lewis I, Jirik F R, Characterization and kinetic analysis of the intracellular domain of human protein tyrosine phosphatase beta (HPTP beta) using synthetic phosphopeptides., Biochemical Journal, 298 ( Pt 2), 395-401, 1 Mar 1994 Abstract
  • Mastick C C, Aebersold R, Lienhard G E, Characterization of a major protein in GLUT4 vesicles. Concentration in the vesicles and insulin-stimulated translocation to the plasma membrane., Journal of Biological Chemistry, 269(8), 6089-92, 25 Feb 1994 Abstract
  • Gold M R, Duronio V, Saxena S P, Schrader J W, Aebersold R, Multiple cytokines activate phosphatidylinositol 3-kinase in hemopoietic cells. Association of the enzyme with various tyrosine-phosphorylated proteins., Journal of Biological Chemistry, 269(7), 5403-12, 18 Feb 1994 Abstract
  • Gold M R, Aebersold R, Both phosphatidylinositol 3-kinase and phosphatidylinositol 4-kinase products are increased by antigen receptor signaling in B cells., Journal of Immunology, 152(1), 42-50, 1 Jan 1994 Abstract
  • Watts J D, Welham M J, Kalt L, Schrader J W, Aebersold R, IL-2 stimulation of T lymphocytes induces sequential activation of mitogen-activated protein kinases and phosphorylation of p56lck at serine-59., Journal of Immunology, 151(12), 6862-71, 15 Dec 1993 Abstract
  • Watts J D, Sanghera J S, Pelech S L, Aebersold R, Phosphorylation of serine 59 of p56lck in activated T cells., Journal of Biological Chemistry, 268(31), 23275-82, 5 Nov 1993 Abstract
  • van Oers N S, Tao W, Watts J D, Johnson P, Aebersold R, Teh H S, Constitutive tyrosine phosphorylation of the T-cell receptor (TCR) zeta subunit: regulation of TCR-associated protein tyrosine kinase activity by TCR zeta., Molecular and Cellular Biology, 13(9), 5771-80, September 1993 Abstract
  • Hess D, Covey T C, Winz R, Brownsey R W, Aebersold R, Analytical and micropreparative peptide mapping by high performance liquid chromatography/electrospray mass spectrometry of proteins purified by gel electrophoresis., Protein Science, 2(8), 1342-51, August 1993 Abstract
  • Aebersold R, Mass spectrometry of proteins and peptides in biotechnology., Current Opinion In Biotechnology, 4(4), 412-9, August 1993 Abstract
  • Wang Q, Tull D, Meinke A, Gilkes N R, Warren R A, Aebersold R, Withers S G, Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase., Journal of Biological Chemistry, 268(19), 14096-102, 5 Jul 1993 Abstract
  • Daga A, Micol V, Hess D, Aebersold R, Attardi G, Molecular characterization of the transcription termination factor from human mitochondria., Journal of Biological Chemistry, 268(11), 8123-30, 15 Apr 1993 Abstract
  • Keller S R, Aebersold R, Garner C W, Lienhard G E, The insulin-elicited 160 kDa phosphotyrosine protein in mouse adipocytes is an insulin receptor substrate 1: identification by cloning., Biochimica Et Biophysica Acta, 1172(3), 323-6, 20 Mar 1993 Abstract
  • Harrington M G, Aebersold R, Martin B M, Merril C R, Hood L, Identification of a brain-specific human cerebrospinal fluid glycoprotein, beta-trace protein., Applied and Theoretical Electrophoresis, 3(5), 229-34, 1993 Abstract
  • Kim S, Sandusky P, Bowlby N R, Aebersold R, Green B R, Vlahakis S, Yocum C F, Pichersky E, Characterization of a spinach psbS cDNA encoding the 22 kDa protein of photosystem II., Febs Letters, 314(1), 67-71, 7 Dec 1992 Abstract
  • Green B R, Shen D, Aebersold R, Pichersky E, Identification of the polypeptides of the major light-harvesting complex of photosystem II (LHCII) with their genes in tomato., Febs Letters, 305(1), 18-22, 22 Jun 1992 Abstract
  • Gebler J C, Aebersold R, Withers S G, Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli., Journal of Biological Chemistry, 267(16), 11126-30, 5 Jun 1992 Abstract
  • Liu M S, Aebersold R, Fann C H, Lee C Y, Molecular and developmental studies of a sperm acrosome antigen recognized by HS-63 monoclonal antibody., Biology of Reproduction, 46(5), 937-48, May 1992 Abstract
  • Aebersold R, Bures E J, Namchuk M, Goghari M H, Shushan B, Covey T C, Design, synthesis, and characterization of a protein sequencing reagent yielding amino acid derivatives with enhanced detectability by mass spectrometry., Protein Science, 1(4), 494-503, April 1992 Abstract
  • Patterson S D, Hess D, Yungwirth T, Aebersold R, High-yield recovery of electroblotted proteins and cleavage fragments from a cationic polyvinylidene fluoride-based membrane., Analytical Biochemistry, 202(1), 193-203, April 1992 Abstract
  • Ettehadieh E, Sanghera J S, Pelech S L, Hess-Bienz D, Watts J, Shastri N, Aebersold R, Tyrosyl phosphorylation and activation of MAP kinases by p56lck., Science, 255(5046), 853-5, 14 Feb 1992 Abstract
  • Watts J D, Wilson G M, Ettenhadieh E, Clark-Lewis I, Kubanek C A, Astell C R, Marth J D, Aebersold R, Purification and initial characterization of the lymphocyte-specific protein-tyrosyl kinase p56lck from a baculovirus expression system., Journal of Biological Chemistry, 267(2), 901-7, 15 Jan 1992 Abstract
  • Gilkes N R, Claeyssens M, Aebersold R, Henrissat B, Meinke A, Morrison H D, Kilburn D G, Warren R A, Miller R C Jr, Structural and functional relationships in two families of beta-1,4-glycanases., European Journal of Biochemistry, 202(2), 367-77, 5 Dec 1991 Abstract
  • Aebersold R, Watts J D, Morrison H D, Bures E J, Determination of the site of tyrosine phosphorylation at the low picomole level by automated solid-phase sequence analysis., Analytical Biochemistry, 199(1), 51-60, 15 Nov 1991 Abstract
  • Schwartz E, Stasys R, Aebersold R, McGrath J M, Green B R, Pichersky E, Sequence of a tomato gene encoding a third type of LHCII chlorophyll a/b-binding polypeptide., Plant Molecular Biology, 17(4), 923-5, October 1991 Abstract
  • Tull D, Withers S G, Gilkes N R, Kilburn D G, Warren R A, Aebersold R, Glutamic acid 274 is the nucleophile in the active site of a "retaining" exoglucanase from Cellulomonas fimi., Journal of Biological Chemistry, 266(24), 15621-5, 25 Aug 1991 Abstract
  • Babcook J, Watts J, Aebersold R, Ziltener H J, Automated nonisotopic assay for protein-tyrosine kinase and protein-tyrosine phosphatase activities., Analytical Biochemistry, 196(2), 245-51, 1 Aug 1991 Abstract
  • Keller S R, Kitagawa K, Aebersold R, Lienhard G E, Garner C W, Isolation and characterization of the 160,000-Da phosphotyrosyl protein, a putative participant in insulin signaling., Journal of Biological Chemistry, 266(20), 12817-20, 15 Jul 1991 Abstract
  • Pichersky E, Subramaniam R, White M J, Reid J, Aebersold R, Green B R, Chlorophyll a/b binding (CAB) polypeptides of CP29, the internal chlorophyll a/b complex of PSII: characterization of the tomato gene encoding the 26 kDa (type I) polypeptide, and evidence for a secon, Molecular and General Genetics, 227(2), 277-84, June 1991 Abstract
  • Patel S D, Aebersold R, Attardi G, cDNA-derived amino acid sequence of the NADH-binding 51-kDa subunit of the bovine respiratory NADH dehydrogenase reveals striking similarities to a bacterial NAD(+)-reducing hydrogenase., Proceedings of the National Academy of Sciences (USA), 88(10), 4225-9, 15 May 1991 Abstract
  • Posada J, Sanghera J, Pelech S, Aebersold R, Cooper J A, Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts., Molecular and Cellular Biology, 11(5), 2517-28, May 1991 Abstract
  • Clark-Lewis I, Moser B, Walz A, Baggiolini M, Scott G J, Aebersold R, Chemical synthesis, purification, and characterization of two inflammatory proteins, neutrophil activating peptide 1 (interleukin-8) and neutrophil activating peptide., Biochemistry, 30(12), 3128-35, 26 Mar 1991 Abstract
  • Schwartz E, Shen D, Aebersold R, McGrath J M, Pichersky E, Green B R, Nucleotide sequence and chromosomal location of Cab11 and Cab12, the genes for the fourth polypeptide of the photosystem I light-harvesting antenna (LHCI)., Febs Letters, 280(2), 229-34, 25 Mar 1991 Abstract
  • Wettenhall R E, Aebersold R H, Hood L E, Solid-phase sequencing of 32P-labeled phosphopeptides at picomole and subpicomole levels., Methods In Enzymology, 201, 186-99, 1991 Abstract
  • Sanghera J S, Aebersold R, Morrison H D, Bures E J, Pelech S L, Identification of the sites in myelin basic protein that are phosphorylated by meiosis-activated protein kinase p44mpk., Febs Letters, 273(1-2), 223-6, 29 Oct 1990 Abstract
  • Aebersold R, Morrison H D, Analysis of dilute peptide samples by capillary zone electrophoresis., Journal of Chromatography, 516(1), 79-88, 7 Sep 1990 Abstract
  • Kalmar G B, Kay R J, Lachance A, Aebersold R, Cornell R B, Cloning and expression of rat liver CTP: phosphocholine cytidylyltransferase: an amphipathic protein that controls phosphatidylcholine synthesis., Proceedings of the National Academy of Sciences (USA), 87(16), 6029-33, August 1990 Abstract
  • Aebersold R, Leavitt J, Sequence analysis of proteins separated by polyacrylamide gel electrophoresis: towards an integrated protein database., Electrophoresis, 11(7), 517-27, July 1990 Abstract
  • Aebersold R, Pipes G D, Wettenhall R E, Nika H, Hood L E, Covalent attachment of peptides for high sensitivity solid-phase sequence analysis., Analytical Biochemistry, 187(1), 56-65, 15 May 1990 Abstract
  • Lin C S, Aebersold R H, Leavitt J, Correction of the N-terminal sequences of the human plastin isoforms by using anchored polymerase chain reaction: identification of a potential calcium-binding domain., Molecular and Cellular Biology, 10(4), 1818-21, April 1990 Abstract
  • Aebersold R, Solid-phase protein sequence analysis., Nature, 343(6255), 291-2, 18 Jan 1990 Abstract
  • Withers, S.G., and R. Aebersold, Approaches to Labeling and Identification of Active Site Residues in Glycosidases, Protein Science, 1995
  • Hess, D., H. Nika, E.J. Bures, D. Chow, H.D. Morrison, R. Aebersold, Liquid Chromatography-electrospray ionization mass spectrometry of 4-(3-pyridinylmethylaminocarboxypropyl) phenylthiohydantoins, Ana. Biochem., 224:373-381, 1995
  • Bures, E.J., H. Nika, D. Hess, H.D. Morrison, R. Aebersold, Synthesis of the Protein Sequencing Reagent 4-(3-pyridinylmethyl-aminocarboxypropyl) Phenyl Isothiocyanate and Characterization of 4-(3-pyridinylmethylaminocarboxypropyl) Phenylthiohydantoins, Anal. Biochem., 224:364-372, 1995
  • Amankwa, L.N., K. Harder, F. Jirik, R. Aebersold, High Sensitivity Determination of Sites of Protein Phosphorylation by On-line Capillary Electrophoresis and Electrospray Ionization Mass Spectrometry, Protein Science, 4:113-125, 1995
  • Watts, J.D., M. Affolter, D.L. Krebs, R.L. Wange, L.E. Samelson, R. Aebersold, Identification by Electrospray Ionization Mass Spectrometry: The Sites of Tyrosine Phosphorylation Induced in Jurkat T-cells on the Protein Tyrosine Kinase ZAP-70, J. Biol. Chem., 269(47), P 29520-9, 1994

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Last Updated: 9/11/1998

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