Dr. Evangelyn C. Alocilja

powered by
COS Expertise®
Michigan State University
College of Engineering
Biosystems & Agricultural Engineering
Biosystems Engineering
Associate ProfessorAppointed: 2000
Michigan State University
National Center for Food Protection and Defense
Co-investigatorAppointed: 2004
Michigan State University
National Food Safety/Toxicology Center
Associate ProfessorAppointed: 2002
Professional Headshot of Evangelyn C. Alocilja

Mailing Address

Biosystems Engineering Program
Department of Biosystems and Agricultural Engineering
Michigan State University
213 Farrall Hall
East Lansing, Michigan 48824-1323
United States

Contact Information

Phone: (517) 355-0083
Fax: (517) 432-2892
alocilja@msu.edu
http://www.egr.msu.edu/~alocilja/

Qualifications

Ph.D., Michigan State University, Systems Science/Electrical Engineering, 1987.
M.S., Michigan State University, Systems Science/Electrical Engineering, 1983.
M.S., University of the Philippines, Soil Chemistry/Plant Physiology, 1981.
B.S., Silliman University, Chemistry (cum laude), 1973.

Expertise and Research Interests

Focus:
Development of bio-inspired nano-structured biosensors and bioelectronic devices

Applications:
Homeland security, food and environmental safety (biosafety), point-of-care diagnostics, tamper evidence, and anti-counterfeiting

Other Expertise

Sensor-based packaging, sensor-based supply chain, and sensors against counterfeiting

Future Research

Development of bio-inspired multi-array nano-structured biosensors with remote data transmission capabilities for rapid, sensitive, specific, handheld, and point-of-care detection/diagnosis of disease-causing agents and other contaminants.

Industrial Relevance

Biosensors are important to biosecurity, homeland security, food safety, environmental monitoring, medical diagnostics, tamper evidence, and against counterfeiting activities.

Keywords

COS Keywords:

Agricultural Engineering, Biomimetics, Biosensors, Diagnostic Monitoring Systems, Electrical Engineering or Electronics, Engineering Education, Food Safety, Foodborne Diseases, Infectious Diseases or Agents, National Security, Plant Physiology or Morphology, Soil Chemistry, Toxicology.

Additional Terms:

Biosecurity, Biosensors, Diagnostic Monitoring Systems, Environmental Safety, Food Safety, Foodborne and Wateborne Pathogens, Homeland Security, Infectious Diseases, Rapid Detection.

Languages

(Reading, Writing, Speaking)

English: (Fluent, Fluent, Fluent)
Tagalog (Philippine Language): (Fluent, Fluent, Fluent)
Spanish: (Functional, Basic, Basic)

Memberships

American Association for the Advancement of Science
American Society for Engineering Education
American Society of Agricultural and Biological Engineers
Institute of Biological Engineering
Institute of Electrical and Electronics Engineers
Phi Beta Delta
Sigma Xi, The Scientific Research Society

Honors and Awards

2003, Excellence in Teaching Materials and Methods in Biological and Agricultural Engineering, American Society for Engineering Education
1995, Withrow Teaching Excellence Award, College of Engineering, Michigan State University, Teaching

Previous Positions

1992, Visiting Scientist, Commonwealth Scientific and Industrial Research Organization (CSIRO), Water Resources
1991-1993, Research Consultant, International Rice Research Institute, Agroecology
1991, Management Consultant, Louis Berger International, Inc., Feasibility Study and Industrial Master Planning of the Caga
1988-1990, Visiting Assistant Professor, Michigan State University, College of Engineering, Agricultural Engineering

Patents

Biologically enhanced electrically-active magnetic nanoparticles for concentration, separation, and detection applications, Patent Number: MSU 4.1-891 TEC2007-0157-01 Serial No. 12/214325, 2008, United States of America.
DNA-polypyrrole based biosensor for rapid detection of biohazardous agents, Patent Number: , 2005, United States of America.
Nanoporus silicon-based electrochemical DNA biosensor, Patent Number: , 2005, United States of America.
Synthesis of conducto-magnetic polymers as anao-transducers in biosensor design, Patent Number: , 2005, United States of America.
Method and Apparatus for the detection of volatile products in a sample, issued on July 27, 2004, Patent Number: 6767732, 2004, Institution, United States.
Inpedimetric biosensor and it's use for rapid dtection of bacterial pathogens in solution, Michigan State University, Patent Number: , 2004, Afghanistan.
Method and Apparatus for the detection of volatile products in a sample issued on March 25, 2003, Patent Number: 6537802, 2003, Institution, United States.
Method and impedimetric biosensor for rapid detection of bacterial pathogens in solution, Patent Number: Michigan State University #03-060, 2003, (Application filed), United States of America.
Multi-array conductimetric biosensor, Patent Number: Michigan State University ID #02-018, 2003, (Application filed), United States of America.

Funding Received

  • Department of Homeland Security through the National Center for Food Protection and Defense: Development of a multiplex bio-barcode DNA biosensor for Bacillus anthracis detection without PCR amplification, $180,000, 2007 to 2009.
  • Department of Homeland Security through the National Center for Food Protection and Defense: Development of biologically modified electrically-active magnetic nanoparticles (nano-BEAMs) for direct capture and concentration of B. anthracis spores and cells in various food matrices, $150,000, 2007 to 2009.
  • 21st Century Jobs Fund, Michigan Economic Development Corporation: Fundamental Studies on Multifunctional Electrospun Nanofibers for Sensor Applications, $659,677, 2007 to 2009.
  • Environmental Protection Agency (EPA): Rapid and quantitative detection of Helicobacter pylori and E. coli O157 in well water using a nano-wired biosensor and QPCR, $600,000, 2006 to 2009.
  • Michigan Department of Environmental Quality: Rapid detection system for viable E. coli in surface water, $100,228, 2006 to 2008.
  • Center for Water Science: Emerging waterborne viruses in Michigan waters: development of DNA-based detection tools for surveillance and assessing risk, $111,000, 2006 to 2008.
  • National Science Foundation: Development of Forward Error-Correcting Multi-Array Biosensor Based on Molecular Bio-Wires, $270,000, 2006 to 2009.
  • Department of Homeland Security - National Center for Food Protection and Defense: Biosensor development for food protection and defense, $213,743, 2005 to 2008.
  • National Science Foundation (NSF): Nanoscale design of aqueous carbon nanotube-reinforced molecular transducer for biosensor design, $160,000, 2005 to 2006.
  • United States Department of Agriculture (USDA): Development of a Field-based Biosensor to Enhance the Epidemiological Studies of BVDV, $326,000, 2005 to 2007.
  • MAES-USDA: Development of a multi-array biosensor for food safety and bio-security, 2005 to 2006.
  • MAES-USDA: Development and application of biosensors for detection of animal diseases, 2005 to 2006.
  • National Science Foundation: NER: Nanoscale design of aqueous carbon nanotube-reinforced molecular transducers for biosensors, $160,000, 2005 to 2008.
  • Michigan Animal Industry Initiative: Biosensor development for agro-security, Phase II, $25,000, 2004 to 2005.
  • Michigan Animal Industry Initiative: Biosensor development for agro-security, $28,350, 2003 to 2004.
  • USDA-NRICGP: Conductometric biosensor for foodborne pathogen detection in fresh produce, $90,000, 2003 to 2005.
  • USDA: Development of a biosensor for rapid detection of viruses, $14,910, 2002 to 2003.
  • Lemelson Foundation-National Collegiate Inventors and Innovators Alliance: Agen BioSense: Development of a prototype biosensor, $12,575, 2002 to 2004.
  • USDA National Integrated Food Safety Initiative: Chemiluminescence Detection of Microbial Contaminants on Fresh Produce, $325,284, 2000 to 2004.
  • National Pork Producers Council: Rapid Detection of Pathogenic Contamination in Pork for HACCP Monitoring, $18,727, 2000 to 2001.
  • Michigan Animal Industry Initiative: In vivo Detection of E. coli O157:H7 in Lambs as Ruminant Model of Cattle, $48,730, 2000 to 2001.
  • Michigan Animal Industry Initiative: Identification and Differentiation of Foodborne Pathogens Using SPR Biosensor, $8,836, 2000 to .
  • Michigan State University: Development of a "Lab-on-Chip" Biosensor for Food and Environmental Safety, and Biosecurity, 2000 to 2006.
  • USDA Higher Education Challenge Grants: Student Portfolios to Encourage Learning in Biological Engineering, $184,112, 1999 to 2002.
  • Management of Technology and Innovation, College of Engineering: Design Improvement and Marketing Potential of the E. Coli O157:H7 Sniffer, $8,700, 1999 to .
  • USDA-Funds for Rural America (FRA): National Center for Manure and Animal Waste Management, Co-PI, a consortium of 14 US institutions, based at North Carolina State University, $3,840,000, 1999 to 2003.
  • Department of Agricultural Engineering: Use of SPR-based biosensor for the early detection of foodborne pathogens, $3,000, 1999 to 2000.
  • Michigan Plant Initiative Program (GREEEN): Early detection of pathogenic contamination in packaged plant food products as post-harvest HACCP protocol. Part 1. Contamination from Salmonella typhimurium, $80,000, 1999 to 2001.
  • Manure Management - Michigan Animal Industry Initiative: Development of a phosphorus balance swine model, $11,330, 1998 to .
  • Michigan Potato Industry Commission: Development of a low cost, real-time, on-site electronic nose system for rapid and early detection of spoilage in stored potatoes. Part 1. Laboratory model, $5,000, 1998 to 1999.
  • Michigan Animal Initiative Program: Development of a 'breath test' for the early detection of E. coli O157:H7 and other Shiga toxin-producing strains. Part 1. In vitro Detection and Differentiation, $33,094, 1998 to 2000.
  • College of Engineering/Dept. of Agricultural Engineering, Michigan State University: Summer Internship, $4,000, 1997 to .
  • Michigan Animal Industry Initiative: Optimization of Whole-Farm Management for Zero-Excess Manure P and N Nutrients in Dairy-Crop Operations, $89,030, 1996 to 1998.
  • College of Engineering/Dept. of Agricultural Engineering, Michigan State University: Summer Internship, $4,000, 1996 to .
  • Michigan Animal Initiative Program: Phosphorus Balance Model of a Dairy-Crop System, $50,000, 1995 to 1996.
  • USAID: Printing Funds to convert Ph.D. thesis manuscript into a monograph, 1987 to .

Publications

  • Torres-Chavolla, E., Alocilja, E.C. (2008) Aptasensors for detection of microbial and viral pathogens, Biosensors and Bioelectronics
  • Okafor, C.,, Grooms, D.,, Alocilja, E., Bolin, S. (2008) Fabrication of a Novel Conductometric Biosensor for Detecting Mycobacterium avium subsp. paratuberculosis Antibodies, Sensors, 8 (9), 6015-6025
  • Yuk, J.S.,, Jin, J.H.,, Alocilja, E.C.,, Rose, J. (2008) Performance enhancement of polyaniline-based polymeric wire biosensor., Biosensors and Bioelectronics, 24, 1348-1352
  • Zhang, D, Carr, D.J.,, Alocilja, E.C (2008) Fluorescent bio-barcode DNA assay for the detection of Salmonella enterica serovar Enteritidis., Biosensors and Bioelectronics, 24, 1377-1381
  • Pal, S., Alocilja, E.C (2008) Electrically-Active Polyaniline Coated Magnetic (EAPM) Nanoparticle as Novel Transducer in Biosensor for Detection of Bacillus anthracis Spores in Food Samples., Biosensors and Bioelectronics
  • Liu, Y.,, Gore, A.,, Chakrabartty, S.,, Alocilja, E.C. (2008) Characterization of sub-systems of a molecular bio-based biosensor device., Microchimica Acta, 163, 49-56.
  • Jin, J.H.,, Zhang, D, Alocilja, E.C., Grooms, D. (2008) Label-free DNA sensor on nano-porous silicon-polypyrrole chip for monitoring Salmonella species., IEEE Sensors Journal, 8 (6), 891-895.
  • Zhang, D., Alocilja, E.C. (2008) Characterization of nano-porous silicon-based DNA biosensor for the detection of Salmonella Enteritidis., IEEE Sensors Journal, 8 (6), 775-780.
  • Pal, S.,, Setterington, E, Alocilja, E.C (2008) Electrically-Active Magnetic Nanoparticles for Concentrating and Detecting Bacillus anthracis Spores in a Direct-Charge Transfer Biosensor., IEEE Sensors Journal, 8 (6), 647-654.
  • Pal, S.,, Ying, W.,, Alocilja,E.C.,, Downes, F.P. (2008) Sensitivity and Specificity Performance of a Direct-Charge Transfer Biosensor for Detecting Bacillus cereus in Selected Food Matrices., Biosystems Engineering Journal, 99: (4), 461-468
  • Cabaraban, M.T.,, Khire, M.V.,, Alocilja, E.C. (2008) Aerobic in-vessel composting versus bioreactor landfilling using life cycle inventory models., Clean Technologies and Environmental Policy Journal,, 10 (1), 39-52
  • Alocilja, E.C. and, Muhammad-Tahir, Z. (2008) Label-free microbial biosensors based on conducting polymers., In: M. Zuorob et al. (ed). Principles of bacterial detection: biosensors, recognition receptors, and Microsystems,, Springer, New York (bookchapter)
  • Alocilja, E.C (2008) Biosensors for detecting pathogenic bacteria in the meat industry, In: F. Toldrá. (ed.), Meat Biotechnology, Springer, New York (bookchapter)
  • Yang Liu,, Shantanu Chakrabartty, Evangelyn C.Alocilja (2008) A Multiplexed Biosensor based on Biomolecular Nanowires, IEEE International Symposium on Circuits and Systems , Seattle, USA
  • Liu, Y.,, Chakrabartty, S.,, Alocilja, E.C. (2007) Fundamental building blocks for molecular bio-wire based forward-error correcting biosensors,, Nanotechnology Journal, 18, 4240-4217
  • Alocilja, E.C (2007) Case Study on Problem-based Learning in a Bio-resource Optimization Course., International Journal of Engineering Education, 1-6
  • Muhammad-Tahir, Z., Alocilja, E.C.,, Grooms, D.L (2007) Indium Tin Oxide-Polyaniline Biosensor: Fabrication and Characterization, Sensors Journal, 7, 1123-1140.
  • Pal, S., Alocilja,E.C., Downes, F.P. (2007) Nanowire Labeled Direct-Charge Transfer Biosensor for Detecting Bacillus species, Biosensors and Bioelectronics, 22, 2329-2336
  • Jin, J.H.,, Wong, K.,, Xagoraraki, I.,, Rose, J.B.,, Alocilja, E.C. (2007) Label Free DNA-Porous Silicon Biosensor for the Detection of Enteric Adenoviruses,, 107th General Meeting of the American Society for Microbiology, Toronto, ON, Canada
  • Liu, Y.,, Gore, A.,, Chakrabartty, S.,, Alocilja, E.C. (2007) A Molecular Bio-wire based Multi-array Biosensor with Integrated potentiostats, Proceedings of the 2007 IEEE Biomedical Circuits and Systems Conference,Montréal, Canada, Nov.2007, 29-32 pages
  • Okafor, C.C.,, Grooms, D.L.,, Alocilja, E.C. (2007) Detection of Mycobacterium avium paratuberculosis antibodies in bovine serum using a conductometric biosensor., International Colloquium on Paratuberculosis, Tsukuba International Congress Center, Tsukuba, Japan, Oct. 29-Nov. 2
  • Pal, S.,, Hummel, E. B.,, Alocilja, E. C (2007) Synthesis of a nano-electromagnetic polymer and its application in a DCT biosensor, NSTI Nanotech Conference and Trade Show, 2007, 485-488 pages
  • Alocilja, E.C, McLean, T (2007) Bioluminescent Sensors for Onsite Non-specific Screening of Toxic Agents in Food and Water, IEEE Conference on Technologies for Homeland Security, 16-17 May 2007, Boston-Woburn, MA, 204-208 pages
  • Zuo, Y., Chakrabartty, S., Muhammad-Tahir, Z., Pal, S., and Alocilja, E.C. (2006) Spatio-temporal Processing for Multi-channel Biosensors using Support Vector Machines, IEEE Sensors Journal, 6 (6), 1644-1651
  • Kindschy, L., Alocilja, E.C. (2006) Development of a molecularly imprinted biomimetic sensor, Biotechnology Progress, 7, 1630-1642
  • Gore, A., Chakrabartty, S., Pal, S., Alocilja, E.C. (2006) A multi-channel femtoampere-sensitivity potentiostat array for biosensing applications, IEEE Sensors Journal, 53 (11), 2357-2363
  • Meeusen, C, Alocilja, E, Osburn, W. (2005) Detection of E. coli O157:H7 Using Miniaturized Surface Plasmon Resonance Biosensor, Transactions of the ASAE, 48 (6), 2409-2416
  • Rodriguez-Lopez, M, Alocilja, E.C. (2005) Embedded DNA-polypyrrole model biosensor for rapid detection of Escherichia coli, IEEE Sensors Journal, 5 (4), 733-736
  • Muhammad-Tahir, Z, Alocilja, E.C., Grooms D.L. (2005) Rapid detection of bovine viral diarrhea virus as surrogate of bioterrorism agents, IEEE Sensors Journal, 5 (4), 757-762
  • Radke, S, Alocilja, E.C. (2005) A Microfabricated Biosensor for Detecting Foodborne Bioterrorism Agents, IEEE Sensors Journal, 5 (4), 744-750
  • Mathew, F. and Alocilja, E.C. (2005) Porous silicon-based biosensor for pathogen detection, Biosensors and Bioelectronics Journal, 20 (8), 1656-1661
  • Kindschy, L., Alocilja E.C. (2005) A molecularly imprinted polymer on indium tin oxide and silicon, Biosensors and Bioelectronics Journal, 20 (10), 2163-2167
  • Radke, S, Alocilja, E.C. (2005) A High Density Microelectrode Array Biosensor for Detection of E. coli O157:H7, Biosensors and Bioelectronics Journal, 20 (8), 1662-1667
  • Muhammad-Tahir, Z, Alocilja, E.C., Grooms D. (2005) Polyaniline synthesis and its application in biosensor, Biosensors and Bioelectronics Journal, 20 (8), 1690-1695
  • Mathew, F, Alagesan, D, Alocilja, E.C. (2004) Chemiluminescence detection of viable Escherichia coli in fresh produce obtained from different sources, Journal of Luminescence, 19, 193-198
  • Radke, S, Alocilja, E.C. (2004) Design and Fabrication of a Micro-Impedance Biosensor for Bacterial Detection, IEEE Sensors Journal, 4 (4), 434-440
  • Muhammad-Tahir, Z, Alocilja, E.C. (2004) A Disposable biosensor for pathogen detection in fresh produce samples, Biosystems Engineering Journal, 88 (2), 145-151
  • Mathew, F, Alocilja, E.C. (2004) Enzyme-based detection of Escherichia coli, Transactions of the ASAE, 47 (1), 357-362
  • Kindschy, L. and Alocilja, E.C. (2004) A Review of Molecularly Imprinted Polymers for Biosensor Development for Food and Agricultural Applications, Transactions of the ASAE, 47 (4), 1375-1382
  • Radke, S. and Alocilja, E.C. (2004) Development of E-team to Commercialize Engineering Research, 2004 ASEE Conference, Salt Lake City, Utah, ASEE Publications
  • Alocilja, E.C (2004) Development of biologically active organic nanotransducers, 2004 Joint International Meeting - 206th Meeting of the Electrochemical Society/2004 Fall Meeting of the Electrochemical Society of Japan,
  • Alocilja, E.C, Ritchie, N, Grooms, D. (2003) Protocol development using an electronic nose for differentiating E. coli strains, IEEE Sensors Journal, 3 (6), 801-805
  • Younts, S, Alocilja, E.C, Osburn, W, Marquie, S, Gray, J, Grooms, D. (2003) Experimental use of a gas sensor-based instrument for differentiation of E. coli O157:H7 from non-O157:H7 E. coli field isolates, Journal of Food Protection, 66 (8), 1455-1458
  • Muhammad-Tahir, Z, Alocilja, E.C (2003) Fabrication of a disposable biosensor for Escherichia coli O157:H7 detection, IEEE Sensors Journal, 3 (4), 345-351
  • Muhammad-Tahir, Z, Alocilja, E.C. (2003) A conductometric biosensor for biosecurity, Biosensors and Bioelectronics Journal, 18 (5-6), 813-819
  • Alocilja, E.C, Radke, S (2003) Market Analysis of Biosensors for Food Safety, Biosensors and Bioelectronics Journal, 18 (5-6), 841-846
  • Mathew, F.P., Alocilja E.C. (2003) Fabrication of porous silicon-based biosensor, IEEE Sensors Conference, October 21-24, 2003, IEEE Publications, 293-298 pages
  • Younts, S., Alocilja, E.C., Osburn, W.N., Marquie, S. Grooms, D.L. (2002) Differentiation of Escherichia coli O157:H7 from non-O157:H7 E. coli serotypes using a sensor-based, computer-controlled detection system, Transactions of the ASAE, 44 (5), 1681-1685
  • Alocilja, E.C. (2002) Principles of Biosystems Engineering, 2nd edition, Erudition Books, ISBN=1-58692-364-1
  • Muhammad-Tahir, Z. and Alocilja, E.C. (2002) Fabrication of a membrane strip immunosensor, IEEE Sensors Conference, Orlando, Florida,12-14 June 2002, IEEE Publications
  • Tuang, F.N., Rademaker, J.L.W., Alocilja, E.C., Louws, F., and DeBruijn, F.J. (1999) Identification of Bacterial rep-PCR Genomic Fingerprints Using a Backpropagation Neural Network, FEMS Microbiology Letters, 177 (2), 249-256
  • Alocilja, E.C (1998) An Optimization Model for Zero-Excess Phosphorus Management, Agricultural Systems Journal, 57 (4), 585-597
  • Alocilja, E.C. (1998) Zero-Excess Manure Management Through Dairy Ration Management, Transactions of the ASAE, 41 (2), 497-501
  • Prathapar, S.A., W.S. Meyer, J.C. Madden, E.C. Alocilja (1997) SWAGMAN Options: A Hierarchical Multicriteria Framework To Identify Profitable Land Uses that Minimize Water Table Rise and Salinization, Journal of Applied Mathematics and Computation, 83, 217-240
  • Alocilja, E.C., Von Bernuth, R.D., and Beede, D.K. (1997) Managing Dairy-Crop Production Systems for Zero-Excess Phosphorus: A Multi-criteria Optimization Approach. In P.S. Teng et al. (eds), Applications of Systems Approaches at the Farm and Regional Levels, Kluwer Academic Publishers (bookchapter)
  • Alocilja, E.C. and J.T. Ritchie (1992) Multicriteria optimization for a sustainable agriculture. In Penning de Vries et al (eds), Systems Approaches for Agricultural Development, Boston, Kluwer Academic Publishers (bookchapter)
  • Alocilja, E.C., J.T. Ritchie, and B. Baer (1991) Structured programming in simulation models In Hodges, T. (eds), Predicting Crop Phenology, Boca Raton, Florida, CRC Press (bookchapter)
  • Alocilja, E.C. and J.T. Ritchie (1991) A model for the phenology of rice In Hodges, T. (eds), Predicting Crop Phenology, Boca Raton, Florida, CRC Press (bookchapter)
  • Alocilja, E.C. and J.T. Ritchie (1990) The application of SIMOPT2:RICE to evaluate profit and risk in rice production, Agricultural Systems Journal, 33, 315-326
  • Alocilja, E.C. (1990) Process Network Theory, IEEE International Conference on Systems, Man, and Cybernetics, IEEE, 66-71 pages
  • Alocilja, E.C. and J.T. Ritchie (1988) Upland rice simulation and its use in multicriteria optimization, Hawaii, IBSNAT Project, 95 pages
  • Ritchie, J.T., E.C. Alocilja, U. Singh, and G. Uehara (1987) IBSNAT and the CERES-Rice model, Weather and Rice, Los Banos, Laguna, Philippines, International Rice Research Institute (bookchapter)

Profile Details

Last Updated: 1/13/2009

COS Expertise ID #151979
Reference this profile directly: http://myprofile.cos.com/alocilja