William Ramses Bishai

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Johns Hopkins University
School of Medicine
Medicine
Infectious Diseases
Associate ProfessorAppointed: 2001
Johns Hopkins University
School of Medicine
Infectious Diseases
Assistant Professor

Mailing Address

J.H. Medical Institutions E. Baltimore
School of Medicine
1503 E. Jefferson St., Room 112
Baltimore, Maryland 21231
United States

Contact Information

Phone: (410) 955-3507
Fax: (410) 614-8173
wbishai@jhmi.edu

Qualifications

M.D., Harvard University, Microbiology and Molecular Genetics, 1989.
Ph.D., Harvard University, Microbiology and Molecular Genetics, 1989.
M.Phil., University of Cambridge, Pharmacology, 1983.
B.A., Harvard College, Biochemistry, 1981.

Expertise and Research Interests

Bacterial pathogenesis
Mycobacterial diseases
Gene regulation
Latency
Dormancy
Sigma factors
Infectious diseases
Genomic sequencing of bacterial pathogens
Tuberculosis
Mycobacterium avium complex (MAC)

Other Expertise

Internal Medicine Residency, Brigham and Women's Hospital, 1989-1991

Keywords

COS Keywords:

Gene Regulation, Infectious Diseases Or Agents, Pathogenesis, Tuberculosis.

Funding Received

  • National Institutes of Health (NIH): New Animal Models for Tuberculosis, 7/15/03 to 7/14/10.
  • National Institute of Allergy and Infectious Diseases: Cell division control and virulence in mycobacteria, 7/1/01 to 6/30/06.
  • National Institute of Allergy and Infectious Diseases: Toolbox for tuberculosis drug discovery, 7/01/03 to 6/30/07.
  • The Ellison Medical Foundation Senior Scientist Award: Transmission blocking vacciines for tuberculosis, 10/1/01 to 9/30/05.
  • National Institute of Allergy and Infectious Diseases: M. Tb survival regulatory genes, 1/1/04 to 12/31/08.
  • National Institute of Allergy and Infectious Diseases: A Search for Genetic Regulators of Latency in M. Tuberculosis, 09/30/94 to 07/31/99.
  • Hoechst Marion Roussel, Inc.: Trial of Rifapentine for TB Prevention in the Cornell Mouse Model, 08/22/96 to 08/30/97.
  • : Does Rifabutin Lead to Rifampin-resistant TB? An Animal Model Study, Pfizer Inc., 07/01/96 to 06/30/97.
  • Sequella Global Tuberculosis Foundation, Inc.: Rabbit tuberculosis and vaccine development, 02/15/00 to 08/31/03.
  • TB Global Alliance: Pre-clinical development of promising TB drugs and drug regimens in the mouse model, 01/01/02 to 12/31/04.

Publications

  • Karakousis PC, Bishai WR, Dorman SE, Mycobacterium tuberculosis cell envelope lipids and the host immune response, Cell Microbiol, 6, 105-116, 2004
  • Nuermberger EL, Yoshimatsu T, Tyagi S, Bishai WR, Grosset JH, Paucibacillary Tuberculosis in Mice after Prior Aerosol Immunization with Mycobacterium bovis BCG, Infect Immun, 1065-71, 2004
  • Nuermberger E, Bishai WR, The clinical significance of macrolide-resistant Streptococcus pneumoniae: it's all relative, Clin Infect Dis, 38, 99-103, 2004
  • Nuermberger EL, Yoshimatsu T, Tyagi S, et al, Moxifloxacin-containing Regimen Greatly Reduces Time to Culture Conversion in Murine Tuberculosis, Am J Respir Crit Care Med, 169, 421-6, 2004
  • Saviola B, Bishai WR, Method to integrate multiple plasmids into the mycobacterial chromosome, Nucleic Acids Res, 32, e11, 2004
  • Mark A. Yoder, Gyanu Lamichhane, and William Bishai, Cavitary pulmonary tuberculosis: The Hole Grail of Disease Transmission, Current Science, 86, 74, 2004
  • Susan E. Dorman, C Hatem, S Tyagi, K Aird, J Lopez-Molina, M. L. M. Pitt, B. C. Zook, A M. Dannenberg Jr., W.R. Bishai, Yukari C. Manabe, Susceptibility to tuberculosis: Clues from studies with inbred and outbred New Zealand white rabbits, Infect Immun, 72, 1700-05, 2004
  • Saviola B, Woolwine SC, Bishai WR, Isolation of acid induced genes of Mycobacterium tuberculosis using recombinase-based in vivo expression technology, Infect Immun, 71, 1379-88, 2003
  • Ando M, Manabe YC, Converse P, Miyazaki E, Ko C, Harrison R, Murphy JR, Bishai W., Characterization of the Role of the Divalent Metal Ion-Dependent Transcriptional Repressor MntR in the Virulence of Staphylococcus aureus, Infect Immun, 71, 2584-90, 2003
  • Lamichhane G, Zignol M, Blades NJ, Geiman DE, Dougherty A, Grosset J, Broman KW, Bishai WR, A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis, PNAS USA, 100, 7213-18, 2003
  • Manabe YC, Dannenberg AM Jr, Tyagi SK, Hatem CL, Yoder M, Woolwine SC, Zook BC, Pitt ML, Bishai WR, Different strains of Mycobacterium tuberculosis cause various spectrums of disease in the rabbit model of tuberculosis, Infect Immun, 71, 6004-11, 2003
  • Ando M, Yoshimatsu T, Ko C, Converse PJ, Bishai WR., Deletion of Mycobacterium tuberculosis sigma factor E results in delayed time to death with bacterial persistence in lungs of aerosol-infected mice. Infect Immun, Infect Immun, 71(12), 7170-2, 2003
  • 44. Bellete B, Coberly J, Link-Barnes G, Ko C, Chaisson RE, Comstock GW, Bishai WR., Evaluation of a whole-blood interferon-gamma release assay for the detection of Mycobacterium tuberculosis infection in 2 study populations, Clin Infect Dis, 34, 1449-56, 2002
  • Cronin WA, Golub JE, Lathan MJ, Mukasa LN, Hooper N, Razeq JH, BarucMulcahy D, Benjamin WH, Magder LS, Strickland GT, Bishai WR., Molecular epidemiology of tuberculosis in a low- to moderate–incidence state: are contact investigations enough?, Emerg Infect Dis, 8, 1271-9, 2002
  • Fleischmann RD, Alland D, Eisen JA, et al., Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains, 184, 5479-90, 2002
  • Kaushal D, Schroeder BG, Tyagi S, Yoshimatsu T, Scott C, Ko C, Mehrotra J, Carpenter L, Manabe YC, Fleischmann RD, Bishai WR, Distinction between bacterial survival and disease induction defined by an alternate sigma factor regulon of Mycobacterium tuberculosis, Proc Natl Acad Sci USA, 99, 8330-5, 2002
  • Manabe YC, Scott CP, Bishai WR, Naturally attenuated, orally administered Mycobacterium microti as a tuberculosis vaccine is better than subcutaneous M. bovis BCG, Infect Immun, 70, 1566-70, 2002
  • Oursler KK, Moore RD, Bishai WR, Harrington SM, Pope DS, Chaisson RE, Survival of patients with pulmonary tuberculosis: clinical and molecular epidemiologic factors, Clin Infect Dis, 34, 752-9, 2002
  • Park WG, Bishai WR, Chaisson RE, Dorman SE, Performance of the microscopic observation drug susceptibility assay in drug susceptibility testing for Mycobacterium tuberculosis, Journal of Clinical Microbiology, 40, 4750-2, 2002
  • Yoshimatsu T, Nuermberger E, Tyagi S, Bishai WR, Grosset J, Bactericidal activity of increasing daily and weekly doses of moxifloxacin in murine tuberculosis, Antimicrob Agents Chemother, 46, 1875-9, 2002
  • Cronin WA, Golub JE, Magder LS et al., Epidemiologic usefulness of spoligotyping for secondary typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110, Journal of Clinical Microbiology, 39, 3709-11, 2001
  • Golub JE. Cronin WA, Obasanjo OO, et al., Transmission of Mycobacerium tuberculosis through casual contaact with an infectious case, Arch Intern Med, 161, 2254-8, 2001
  • Mehrotra J, Bishai WR., Regulation of virulence genes in Mycobacterium tuberculosis, Int J Med Microbiol, 291, 171-82, 2001
  • Bishai W, Tuberculosis transmission -- rogue pathogen or rogue patient?, Am J Respir Crit Care Med, 164, 1104-5, 2001
  • Wagner, KR, Bishai WR, Issues in the treatment of Mycobacterium tuberculosis in patients with human immunodeficiency virus infection, AIDS, Suppl 5, S203-12, 2001
  • Wendel KA, Alwood KS, Gachuhi R, Chaisson RE, Bishai WR, Paradoxal worsening of tuberculosis in HIV-infected persons, Chest, 120, 193-7, 2001
  • Mazurek GH, LoBue PA, Daley CL, et al., Comparison of a whole-blood interferon gamma assay with tuberculin testing for detecting latent MYcobacterium tuberculosis infection, JAMA, 286, 1740-7, 2001
  • Manabe YC, Dannenberg AM Jr, Bishai WR, What we can learn from the Mycobacterium tuberculosis genome sequencing projects., International Journal of Tuberculosis and Lung Disease, 4(2 Suppl 1), S18-23, 2000 Abstract
  • Sterling TR, Pope DS, Bishai WR, Harrington S, Gershon RR, Chaisson RE, Transmission of Mycobacterium tuberculosis from a cadaver to an embalmer., New England Journal of Medicine, 342(4), 246-8, 27 2000 Abstract
  • Manabe YC, Bishai WR, Latent Mycobacterium tuberculosis-persistence, Nature Medicine, 6, 1327, 2000
  • Gomez JE, Bishai WR, whmd is an essential mycobacterial gene required for proper septation and cell division, Proc Natl Acad Sci USA, 97, 8554-9, 2000
  • Dannenberg AM, Bishai WR, Parrish N, et al., Efficacies of BCG and vole bacillus (Mycobacterium microti) vaccines in preventing clinically apparent pulmonary tuberculosis in rabbits: a preliminary report, Vaccine, 19, 796-800, 2000
  • Chen P, Ruiz RE, Li Q, Silver RF, Bishai WR, Construction and characterizatio of a Mycobacterium tuberculosis mutant lacking the alternate sigma factor gene, sigF, Infection and Immunity, 68, 5575-80, 2000
  • Bishai WR, Lipid lunch for persistent pathogen, Nature, 406, 683-5, 2000
  • Bishai WR, Rekindling old controversy on elusive lair of latent tuberculosis, The Lancet, 356, 2113-4, 2000
  • Bishai WR, Is drug resistance affecting treatment outcomes?, Am J Manag Care, 6, S2297-201, 2000
  • Manabe YC, Chen JM, Ko CG, Chen P, Bishai WR, Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologu, Journal of Bacteriology, 181(24), 7629-33, December 1999 Abstract
  • Sterling TR, Alwood K, Gachuhi R, Coggin W, Blazes D, Bishai WR, Chaisson RE, Relapse rates after short-course \(6-month\) treatment of tuberculosis in HIV-infected and uninfected persons., AIDS, 13(14), 1899-904, 1 Oct 1999 Abstract
  • Bishai WR, Dannenberg AM Jr, Parrish N, Ruiz R, Chen P, Zook BC, Johnson W, Boles JW, Pitt ML, Virulence of Mycobacterium tuberculosis CDC1551 and H37Rv in rabbits evaluated by Lurie''s pulmonary tubercle count method., Infection and Immunity, 67(9), 4931-4, September 1999 Abstract
  • Miyazaki E, Chen J M, Ko C, Bishai W R, The Staphylococcus aureus rsbW (orf159) gene encodes an anti-sigma factor of SigB, JOURNAL OF BACTERIOLOGY, 181(9), 2846-51, 1999 Abstract
  • Miyazaki E, Chen JM, Ko C, Bishai WR, The Staphylococcus aureus rsbW \(orf159\) gene encodes an anti-sigma factor of SigB., Journal of Bacteriology, 181(9), 2846-51, May 1999 Abstract
  • Kimura M, Converse PJ, Astemborski J, Rothel JS, Vlahov D, Comstock GW, Graham NM, Chaisson RE, Bishai WR, Comparison between a whole blood interferon-gamma release assay and tuberculin skin testing for the detection of tuberculosis infection among patients at risk for tuberculosis exposure., Journal of Infectious Diseases, 179(5), 1297-300, May 1999 Abstract
  • Michele T M, Ko C, Bishai W R, Exposure to antibiotics induces expression of the Mycobacterium tuberculosis sigF gene: implications for chemotherapy against mycobacterial persistors, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 43(2), 218-25, 1999 Abstract
  • Michele TM, Ko C, Bishai WR, Exposure to antibiotics induces expression of the Mycobacterium tuberculosis sigF gene: implications for chemotherapy against mycobacterial persistors., Antimicrobial Agents and Chemotherapy, 43(2), 218-25, February 1999 Abstract
  • Bishai W, The Mycobacterium tuberculosis genomic sequence: anatomy of a master adaptor., Trends in Microbiology, 6(12), 464-5, December 1998 Abstract
  • Bishai W R, Graham N M, Harrington S, Pope D S, Hooper N, Astemborski J, Sheely L, Vlahov D, Glass G E, Chaisson R E, Molecular and geographic patterns of tuberculosis transmission after 15 years of directly observed therapy see comments, JAMA, 280(19), 1679-84, 18 1998 Abstract
  • Chen P, Bishai W R, Novel selection for isoniazid (INH) resistance genes supports a role for NAD -binding proteins in mycobacterial INH resistance, INFECTION AND IMMUNITY, 66(11), 5099-106, 1998 Abstract
  • Calamita G, Kempf B, Bonhivers M, Bishai W R, Bremer E, Agre P, Regulation of the Escherichia coli water channel gene aqpZ., Proceedings of the National Academy of Sciences (USA), 95(7), 3627-31, 31 Mar 1998 Abstract
  • Parrish N M, Dick J D, Bishai W R, Mechanisms of latency in Mycobacterium tuberculosis, TRENDS IN MICROBIOLOGY, 6(3), 107-12, 1998 Abstract
  • Michele T M, Cronin W A, Graham N M, Dwyer D M, Pope D S, Harrington S, Chaisson R E, Bishai W R, Transmission of Mycobacterium tuberculosis by a fiberoptic bronchoscope Identification by DNA fingerprinting see comments, JAMA, 278(13), 1093-5, 1 1997 Abstract
  • Calamita G, Kempf B, Rudd K E, Bonhivers M, Kneip S, Bishai W R, Bremer E, Agre P, The aquaporin-Z water channel gene of Escherichia coli: structure, organization and phylogeny., Biology of The Cell, 89(5-6), 321-9, August 1997 Abstract
  • Bishai W R, Chaisson R E, Short-course chemoprophylaxis for tuberculosis, CLINICS IN CHEST MEDICINE, 18(1), 115-22, 1997 Abstract
  • DeMaio J, Zhang Y, Ko C, Bishai W R, Mycobacterium tuberculosis sigF is part of a gene cluster with similarities to the Bacillus subtilis sigF and sigB operons, TUBERCLE AND LUNG DISEASE, 78(1), 3-12, 1997 Abstract
  • Gomez J E, Chen J M, Bishai W R, Sigma factors of Mycobacterium tuberculosis, TUBERCLE AND LUNG DISEASE, 78(3-4), 175-83, 1997 Abstract
  • Jacobs W, Brennan P, Curlin G, Ginsberg A, Adams M, Fleischmann R, Fraser C, Venter J C, Shinnick T, Bishai W, Smith H, Stover K, Hatfull G, Comparative sequencing [letter; comment], Science, 274(5284), 17-8, 4 Oct 1996 Abstract
  • Bishai W R, Graham N M, Harrington S, Page C, Moore-Rice K, Hooper N, Chaisson R E, Brief report: rifampin-resistant tuberculosis in a patient receiving rifabutin prophylaxis, NEW ENGLAND JOURNAL OF MEDICINE, 334(24), 1573-6, 13 1996 Abstract
  • DeMaio J, Zhang Y, Ko C, Young D B, Bishai W R, A stationary-phase stress-response sigma factor from Mycobacterium tuberculosis, Proceedings of the National Academy of Sciences (USA), 93(7), 2790-4, 2 1996 Abstract
  • Bishai WR, Graham NMH, Harrinton S, Page C, Moore-ice K, Hooper N, and Chaisson RE., Rifampin-resistant tuberculosis in a patient receiving rifabutin prophylaxis, N. Engl. J. Med., 334, 1573-6, 1996
  • DeMaio J, Zhang Y, Ko C, Young DB, Bishai WR, A stationary phase stress response sigma factor from Nycobacterium tuberculosis, Proc. Natl. Acad. Sci., 93, 2790-4, 1996
  • Calamita G, Bishai W R, Preston G M, Guggino W B, Agre P, Molecular cloning and characterization of AqpZ, a water channel from Escherichia coli, JOURNAL OF BIOLOGICAL CHEMISTRY, 270(49), 29063-6, 8 1995 Abstract
  • Howard N S, Gomez J E, Ko C, Bishai W R, Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector, GENE, 166(1), 181-2, 1 1995 Abstract
  • Calamita G, Bishai WR, Preston GM, Guggino WB, and Agre P., Molecular cloning and characterization of aquaporin Z: a water channel from E.coli., Journal of Bioligical Chemistry, 270, 29063-29066, 1995
  • Howard NS, Gomez JE, Ko C, and Bishai WR., Color selection with a hydromycin-resistance based E.coli-mycobacterial shuttle vector, Gene, 166, 181-182, 1995
  • Bishai W R, Howard N S, Winkelstein J A, Smith H O, Characterization and virulence analysis of catalase mutants of Haemophilus influenzae., Infection and Immunity, 62(11), 4855-60, November 1994 Abstract
  • Bishai W R, Smith H O, Barcak G J, A peroxide/ascorbate-inducible catalase from Haemophilus influenzae is homologous to the Escherichia coli katE gene product., Journal of Bacteriology, 176(10), 2914-21, May 1994 Abstract
  • Bishai WR, Howard NS, Winkelstein JA, and Smith HO, Characterization and virulence analysis of catalase mutants of Haemophilus influenzae, Infect. Imun., 62, 4855-4860, 1994
  • Cha J, Bishai W, Chandrasegaran S, New vectors for direct cloning of PCR products published erratum appears in Gene 1994 Apr 8;141(1):149, GENE, 136(1-2), 369-70, 22 1993 Abstract
  • Bishai W R, Sears C L, Food poisoning syndromes, GASTROENTEROLOGY CLINICS OF NORTH AMERICA, 22(3), 579-608, 1993 Abstract
  • Cronin WA, Golub JE, Lathan MJ, Mukasa LN, Hooper N, Razeq JH, Baruch NG, Mulcahy D, Benjamin WH, Magder LS, Strickland GT, Bishai WR., Molecular epidemiology of tuberculosis in a low- to moderate–incidence state: are contact investigations enough?, Emerg Infect Dis, 8, 1271-9
  • 47. Kaushal D, Schroeder BG, Tyagi S, Yoshimatsu T, Scott C, Ko C, Mehrotra J, Carpenter L, Manabe YC, Fleischmann RD, Bishai WR, Distinction between bacterial survival and disease induction defined by an alternate sigma factor regulon of Mycobacterium tuberculosis, Proc Natl Acad Sci USA, 99, 8330-5
  • Kaushal D, Schroeder BG, Tyagi S, Yoshimatsu T, Scott C, Ko C, Mehrotra J, Carpenter L, Manabe YC, Fleischmann RD, Bishai WR, Distinction between bacterial survival and disease induction defined by an alternate sigma factor regulon of Mycobacterium tuberculosis, Proc Natl Acad Sci USA, 99, 8330-5

Profile Details

Last Updated: 3/15/2004

COS Expertise ID #410067
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