Dr. Janet L. Brandsma

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Yale University
School of Medicine
Comparative Medicine
Associate Professor

Mailing Address

117 LSOG
Yale University
New Haven, Connecticut 06520-8016
United States

Contact Information

Phone: (203) 785-4401
janet.brandsma@yale.edu

Qualifications

Ph.D., Yale University, Virology/Epidemiology, 1981.
M.A., Wesleyan University, Teaching, 1971.
A.B., Syracuse University, French, 1970.

Expertise and Research Interests

Human papillomaviruses (HPVs) are etiologically involved in inducing cervical and other anogenital cancers. Our goals are: (1) to define the genetic determinants of papillomavirus-induced benign and malignant disease, and (2) to develop a papillomavirus vaccine to prevent tumors from forming, and/or to induce, or accelerate, their regression. To address these goals, we utilize the well characterized cottontail papillomavirus (CRPV)-rabbit system. For goal 1: we generate site specific mutations in a complete CRPV genome, test the ability of the mutant genomes to induce disease in rabbits, and characterize lesions that form histologically and biochemically. For goal 2, we generate, and then bind to fine gold particles, eukaryotic expression vectors for individual CRPV genes. Rabbits are immunized with the DNA-coated particles (the candidate vaccine) using an Accell Gene Delivery System. The rabbit immune response is characterized, and its effectiveness is evaluated by challenge with CRPV.

Other Expertise

I wish to be contacted by interested students (medical, graduate or undergraduates) as a potential research mentor/thesis advisor.

Keywords

COS Keywords:

Cancer Biology, Cancer or Carcinogenesis, Cancer Prevention, Dermatology, Genetics, Immunotherapy, Skin Diseases, Vaccine, Virology.

Additional Terms:

CRPV, Cancer, Cancer Biology, Cancer Prevention, Cancer Vaccine, Cancer or Carcinogenesis, Carcinoma, Cervical Cancer, Cottontail Rabbit Papillomavirus, Dermatology, Genital Wart, Health of Underserved Populations, Human Papillomavirus, Immunotherapy, Papillomavirus, Skin Diseases, Vaccine, Vaccines, Virology.

Publications

  • Du M, Fan X, Hanada T, Gao H, Lutchman M, Brandsma JL, Chishti AH, Chen JJ (Apr 2005) Association of cottontail rabbit papillomavirus E6 oncoproteins with the hDlg/SAP97 tumor suppressor., Journal of Cellular Biochemistry, 94 (5), 1038-45 Abstract
  • Cody V, Shen H, Shlyankevich M, Tigelaar RE, Brandsma JL, Hanlon DJ (Feb 2005) Generation of dendritic cells from rabbit bone marrow mononuclear cell cultures supplemented with hGM-CSF and hIL-4., Veterinary Immunology and Immunopathology, 103 (3-4), 163-72 Abstract
  • Peh WL, Brandsma JL, Christensen ND, Cladel NM, Wu X, Doorbar J (Feb 2004) The viral E4 protein is required for the completion of the cottontail rabbit papillomavirus productive cycle in vivo., Journal of Virology, 78 (4), 2142-51 Abstract
  • Brandsma JL, Shlyankevich M, Zhang L, Slade MD, Goodwin EC, Peh W, Deisseroth AB (Jan 2004) Vaccination of rabbits with an adenovirus vector expressing the papillomavirus E2 protein leads to clearance of papillomas and infection., Journal of Virology, 78 (1), 116-23 Abstract
  • Peh WL, Middleton K, Christensen N, Nicholls P, Egawa K, Sotlar K, Brandsma J, Percival A, Lewis J, Liu WJ, Doorbar J (Oct 2002) Life cycle heterogeneity in animal models of human papillomavirus-associated disease., Journal of Virology, 76 (20), 10401-16 Abstract
  • Reuter JD, Vivas-Gonzalez BE, Gomez D, Wilson JH, Brandsma JL, Greenstone HL, Rose JK, Roberts A (Sep 2002) Intranasal vaccination with a recombinant vesicular stomatitis virus expressing cottontail rabbit papillomavirus L1 protein provides complete protection against papillomavirus-induced disease., Journal of Virology, 76 (17), 8900-9 Abstract
  • Leachman SA, Shylankevich M, Slade MD, Levine D, Sundaram RK, Xiao W, Bryan M, Zelterman D, Tiegelaar RE, Brandsma JL (Aug 2002) Ubiquitin-fused and/or multiple early genes from cottontail rabbit papillomavirus as DNA vaccines., Journal of Virology, 76 (15), 7616-24 Abstract
  • Reuter JD, Gomez D, Brandsma JL, Rose JK, Roberts A (Nov 2001) Optimization of cottontail rabbit papilloma virus challenge technique., Journal of Virological Methods, 98 (2), 127-34 Abstract
  • Fujii T, Brandsma JL, Peng X, Srimatkandada S, Li L, Canaan A, Deisseroth AB (Jan 2001) High and low levels of cottontail rabbit papillomavirus E2 protein generate opposite effects on gene expression., The Journal of Biological Chemistry, 276 (2), 867-74 Abstract
  • Leachman SA, Tigelaar RE, Shlyankevich M, Slade MD, Irwin M, Chang E, Wu TC, Xiao W, Pazhani S, Zelterman D, Brandsma JL (Sep 2000) Granulocyte-macrophage colony-stimulating factor priming plus papillomavirus E6 DNA vaccination: effects on papilloma formation and regression in the cottontail rabbit papillomavirus--rabbit model., Journal of Virology, 74 (18), 8700-8 Abstract
  • Zhang P, Nouri M, Brandsma JL, Iftner T, Steinberg BM (Oct 1999) Induction of E6/E7 expression in cottontail rabbit papillomavirus latency following UV activation., Virology, 263 (2), 388-94 Abstract
  • Zhang P, Nouri M, Brandsma JL, Iftner T, Steinberg BM (Oct 1999) Induction of E6/E7 expression in cottontail rabbit papillomavirus latency following UV activation., Virology, 263 (2), 388-94 Abstract
  • Zhang P, Nouri M, Brandsma JL, Iftner T, Steinberg BM (Oct 1999) Induction of E6/E7 expression in cottontail rabbit papillomavirus latency following UV activation., Virology, 263 (2), 388-94 Abstract
  • Sundaram P, Tigelaar R E, Xiao W, Brandsma J L, Intracutaneous vaccination of rabbits with the E6 gene of cottontail rabbit papillomavirus provides partial protection against virus challenge., Vaccine, 16(6), 613-23, April 1998 Abstract
  • Sundaram P, Tigelaar R E, Brandsma J L, Intracutaneous vaccination of rabbits with the cottontail rabbit papillomavirus (CRPV) L1 gene protects against virus challenge., Vaccine, 15(6-7), 664-71, April 1997 Abstract
  • Xiao W, Brandsma J L, High efficiency, long-term clinical expression of cottontail rabbit papillomavirus (CRPV) DNA in rabbit skin following particle-mediated DNA transfer., Nucleic Acids Research, 24(13), 2620-2, 1 Jul 1996 Abstract
  • Sundaram P, Xiao W, Brandsma J L, Particle-mediated delivery of recombinant expression vectors to rabbit skin induces high-titered polyclonal antisera (and circumvents purification of a protein immunogen)., Nucleic Acids Research, 24(7), 1375-7, 1 Apr 1996 Abstract
  • Sundaram P, Brandsma J L, Rapid, efficient, large-scale purification of unfused, non-denatured E7 protein of cottontail rabbit papillomavirus., Journal of Virological Methods, 57(1), 61-70, March 1996 Abstract
  • Brandsma J L, Brownstein D G, Xiao W, Longley B J, Papilloma formation in human foreskin xenografts after inoculation of human papillomavirus type 16 DNA., Journal of Virology, 69(4), 2716-21, April 1995 Abstract
  • Wu X, Xiao W, Brandsma J L, Papilloma formation by cottontail rabbit papillomavirus requires E1 and E2 regulatory genes in addition to E6 and E7 transforming genes., Journal of Virology, 68(9), 6097-102, September 1994 Abstract
  • Brandsma J L, Animal models of human-papillomavirus-associated oncogenesis., Intervirology, 37(3-4), 189-200, 1994 Abstract
  • Gaertner D J, Jacoby R O, Johnson E A, Paturzo F X, Smith A L, Brandsma J L, Characterization of acute rat parvovirus infection by in situ hybridization., Virus Research, 28(1), 1-18, April 1993 Abstract
  • Brandsma J L, Xiao W, Infectious virus replication in papillomas induced by molecularly cloned cottontail rabbit papillomavirus DNA., Journal of Virology, 67(1), 567-71, January 1993 Abstract
  • Brandsma J L, Yang Z H, DiMaio D, Barthold S W, Johnson E, Xiao W, The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits., Journal of Virology, 66(10), 6204-7, October 1992 Abstract
  • Brandsma J L, Yang Z H, Barthold S W, Johnson E A, Use of a rapid, efficient inoculation method to induce papillomas by cottontail rabbit papillomavirus DNA shows that the E7 gene is required., Proceedings of the National Academy of Sciences (USA), 88(11), 4816-20, 1 Jun 1991 Abstract
  • Gaertner D J, Jacoby R O, Paturzo F X, Johnson E A, Brandsma J L, Smith A L, Modulation of lethal and persistent rat parvovirus infection by antibody., Archives of Virology, 118(1-2), 1-9, 1991 Abstract
  • Jacoby R O, Johnson E A, Paturzo F X, Gaertner D J, Brandsma J L, Smith A L, Persistent rat parvovirus infection in individually housed rats., Archives of Virology, 117(3-4), 193-205, 1991 Abstract

Profile Details

Last Updated: 6/21/2007

COS Expertise ID #396883
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