Brad T. Cookson

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University of Washington
Laboratory Medicine and Microbiology
ProfessorAppointed: 2008
University of Washington
UW Medical Center
Clinical Microbiology
HeadAppointed: 2003
University of Washington
School of Medicine
Microbiology
Molecular Diagnosis
DirectorAppointed: 1998
Professional Headshot of Brad T. Cookson

Mailing Address

University of Washington
Dept. of Laboratory Medicine
P.O. Box 357110
Seattle, Washington 98195-7110
United States

Contact Information

Phone: (206) 598-6131
Fax: (206) 598-6189
cookson@u.washington.edu

Qualifications

Diplomate, American Board of Pathology, Clinical Pathology, 1994.
Ph.D., Washington University, 1991.
M.D., Washington University, Medicine, 1991.
B.S., University of Utah, 1983.

Expertise and Research Interests

We use cellular immunology, molecular genetics, biochemistry, and molecular biology to study the interaction of antigen-specific T cells with macrophages infected by facultative intracellular pathogens like Salmonella, Histoplasma, and Mycobacterium. The overall goals are to identify antigens recognized by T cells participating in immunity, define the mechanisms by which T cells help provide immunity, and understand how virulent bacteria escape primary immune responses to cause sepsis.

We also partner with engineers and private industry to utilize and develop biotechnology in two areas. First, to define the mechanism of proinflammatory programmed cell death called pyroptosis (to distinguish it from noninflammatory apoptosis) induced by certain bacterial infections. The pyroptosis pathway is thought to have broad implications for a variety of biological systems, including the immune, cardiovascular, and central nervous systems. The goal is to better understand organ damage resulting from infections, heart attacks, and strokes. Second, to utilize powerful amplification and detection systems to diagnose bacterial and fungal infections. The goal is to improve the medical management of hospitalized patients suffering from infectious diseases.

Keywords

COS Keywords:

Apoptosis, Bacterial Infections, Biochemistry, Cellular Immunology, Fungal Diseases, Genetics, Hospitalized Patient, Immunology, Infectious Diseases Or Agents, Inflammation, Lymphocytes, Macrophage, Medical Microbiology, Microbial Pathogenesis, Microbial Processes, Microbiology, Molecular Biology, Molecular Genetics, Pathogenesis, Pathology, Vaccine.

Additional Terms:

Apoptosis, CD4+ T Cells, Cellular Immunology, Inflammation, Lymphocytes, Macrophage, Medical Microbiology, Microbial Pathogenesis, Molecular Diagnosis, Oral Vaccination to Provide Host Immunity, Programmed Cell Death, Pyroptosis.

Honors and Awards

2007, Chair, Microbial Pathogenesis: Mechanisms of Infectious Diseases, FASEB Summer Research Conference, Snowmass, CO
2005-2007, Alternate Councilor, American Society of Microbiology, Division B (Microbial Pathogenesis)
2004, Vice-chair, Microbial Pathogenesis: Mechanisms of Infectious Diseases, FASEB Summer Research Conference, Snowmass, CO

Previous Positions

2002-2008, Associate Professor, University of Washington, School of Medicine, Microbiology
2002-2008, Associate Professor, University of Washington, School of Medicine, Laboratory Medicine
1996-2002, Assistant Professor, University of Washington, School of Medicine, Microbiology
1996-2002, Assistant Professor, University of Washington, School of Medicine, Laboratory Medicine

Publications

  • Dragavon J, Molter T, Young C, Strovas T, McQuaide S, Holl M, Zhang M, Cookson, Jen A, Lidstrom M, Meldrum D (E-published 6/27/2008) A cellular isolation system for real-time single cell oxygen consumption monitoring, Journal of the Royal Society Interface (DOI: 10.1098/rsif.2008.0106.focus)
  • Fink SL, Bergsbaken T, Cookson BT (Mar 2008) Anthrax lethal toxin and Salmonella elicit the common cell death pathway of caspase-1-dependent pyroptosis via distinct mechanisms., Proceedings of the National Academy of Sciences of the United States of America, 105 (11), 4312-7 Abstract
  • Goodyear N, Ulness BK, Prentice JL, Cookson BT, Limaye AP (2008) Systematic assessment of culture review as a tool to assess errors in the clinical microbiology laboratory., Archives of Pathology and Laboratory Medicine, In Press
  • Alaniz RC, Deatherage BL, Lara JC, Cookson BT (Dec 2007) Membrane Vesicles Are Immunogenic Facsimiles of Salmonella typhimurium That Potently Activate Dendritic Cells, Prime B and T Cell Responses, and Stimulate Protective Immunity In Vivo., Journal of Immunology, 179 (11), 7692-701 Abstract
  • Bergsbaken T, Cookson BT (Nov 2007) Macrophage Activation Redirects Yersinia-Infected Host Cell Death from Apoptosis to Caspase-1-Dependent Pyroptosis., PLoS Pathogens, 3 (11), e161 Abstract
  • Fink SL, Cookson BT (Nov 2007) Pyroptosis and host cell death responses during Salmonella infection., Cellular Microbiology, 9 (11), 2562-70 Abstract
  • Sobhani K, Fink SL, Cookson BT, Dovichi NJ (Jul 2007) Repeatability of chemical cytometry: 2-DE analysis of single RAW 264.7 macrophage cells., Electrophoresis, 28 (13), 2308-13 Abstract
  • Hoffman NG, Cookson BT (Jun 2007) Case 8-2007: a man with chest pain followed by cardiac arrest., The New England Journal of Medicine, 356 (25), 2652-3 Abstract
  • Fink SL, Cookson BT (Nov 2006) Caspase-1-dependent pore formation during pyroptosis leads to osmotic lysis of infected host macrophages., Cellular Microbiology, 8 (11), 1812-25 Abstract
  • Alaniz RC, Cummings LA, Bergman MA, Rassoulian-Barrett SL, Cookson BT (Sep 2006) Salmonella typhimurium coordinately regulates FliC location and reduces dendritic cell activation and antigen presentation to CD4+ T cells., Journal of Immunology, 177 (6), 3983-93 Abstract
  • Cummings LA, Wilkerson WD, Bergsbaken T, Cookson BT (Aug 2006) In vivo, fliC expression by Salmonella enterica serovar Typhimurium is heterogeneous, regulated by ClpX, and anatomically restricted., Molecular Microbiology, 61 (3), 795-809 Abstract
  • Bergman MA, Cummings LA, Alaniz RC, Mayeda L, Fellnerova I, Cookson BT (Nov 2005) CD4+-T-cell responses generated during murine Salmonella enterica serovar Typhimurium infection are directed towards multiple epitopes within the natural antigen FliC., Infection and Immunity, 73 (11), 7226-35 Abstract
  • Rakeman JL, Bui U, Lafe K, Chen YC, Honeycutt RJ, Cookson BT (July 2005) Multilocus DNA sequence comparisons rapidly identify pathogenic molds, Journal of Clinical Microbiology, 43 (7), 3324-33 Abstract
  • Cummings LA, Barrett SL, Wilkerson WD, Fellnerova I, Cookson BT (June 2005) FliC-specific CD4+ T cell responses are restricted by bacterial regulation, Journal of Immunology, 174 (12), 7929-38 Abstract
  • Andersen-Nissen E, Smith KD, Strobe KL, Barrett SL, Cookson BT, Logan SM, Aderem A (Jun 2005) Evasion of Toll-like receptor 5 by flagellated bacteria., Proc Natl Acad Sci USA, 102 (26), 9247-52 Abstract
  • Fink SL, Cookson BT, Apoptosis, Pyroptosis, and Necrosis: Mechanistic Description of Dead And Dying Eukaryotic Cells., Infection and Immunity, 73(4), 1907-16, Apr 2005 Abstract
  • Bergman MA, Cummings LA, Barrett SL, Smith KD, Lara JC, Aderem A, Cookson BT (Mar 2005) CD4+ T cells and toll-like receptors recognize Salmonella antigens expressed in bacterial surface organelles., Infection and Immunity, 73 (3), 1350-6 Abstract
  • Pottumarthy S, Schapiro JM, Prentice JL, Houze YB, Swanzy SR, Fang FC, Cookson BT, Clinical Isolates of Staphylococcus Intermedius Masquerading As Methicillin-resistant Staphylococcus Aureus., Journal of Clinical Microbiology, 42(12), 5881-4, Dec 2004 Abstract
  • Panackal AA, Houze YB, Prentice J, Leopold SS, Cookson BT, Liles WC, Limaye AP, Prosthetic joint infection due to "Helcococcus pyogenica", Journal of Clinical Microbiology, 42(6), 2872-4, Jun 2004 Abstract
  • Vazquez-Torres A, Vallance BA, Bergman M, Finlay BB, Cookson BT, Jones-Carson J, Fang FC (May 2004) Toll-like receptor 4 dependence of innate and adaptive immunity to, Journal of Immunology, 172 (10), 6202-8 Abstract
  • Selvarangan R, Wu WK, Nguyen TT, Carlson LD, Wallis CK, Stiglich SK, Chen YC, Jost KC Jr, Prentice JL, Wallace RJ Jr, Barrett SL, Cookson BT, Coyle MB, Characterization of a novel group of mycobacteria and proposal of Mycobacterium sherrisii sp. nov, Journal of Clinical Microbiology, 42(1), 52-9, Jan 2004 Abstract
  • Selvarangan R, Bui U, Limaye AP, Cookson BT, Rapid identification of commonly encountered Candida species directly from blood culture bottles, Journal of Clinical Microbiology, 41(12), 5660-4, Dec 2003 Abstract
  • Smith KD, Andersen-Nissen E, Hayashi F, Strobe K, Bergman MA, Barrett SL, Cookson BT, Aderem A, Toll-like receptor 5 recognizes a conserved site on flagellin required for protofilament formation and bacterial motility, Nature Immunology, 4(12), 1247-53, Dec 2003 Abstract
  • Novicki TJ, LaFe K, Bui L, Bui U, Geise R, Marr K, Cookson BT, Genetic diversity among clinical isolates of Acremonium strictum determined during an investigation of a fatal mycosis, Journal of Clinical Microbiology, 41(6), 2623-8, June 2003 Abstract
  • Pottumarthy S, Limaye AP, Prentice JL, Houze YB, Swanzy SR, Cookson BT, Nocardia veterana, a new emerging pathogen, Journal of Clinical Microbiology, 41(4), 1705-9, April 2003 Abstract
  • Turgeon DK, Novicki TJ, Quick J, Carlson L, Miller P, Ulness B, Cent A, Ashley R, Larson A, Coyle M, Limaye AP, Cookson BT, Fritsche TR, Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay, Journal of Clinical Microbiology, 41(2), 667-70, February 2003 Abstract
  • Selvarangan R, Limaye AP, Cookson BT, Rapid identification and differentiation of Candida albicans and Candida dubliniensis by capillary-based amplification and fluorescent probe hybridization, Journal of Clinical Microbiology, 40(11), 4308-12, November 2002 Abstract
  • Lingappa JR, Lawrence W, West-Keefe S, Gautom R, Cookson BT, Diagnosis of community-acquired pertussis infection: comparison of both culture and fluorescent-antibody assays with PCR detection using electrophoresis or dot blot hybridization, Journal of Clinical Microbiology, 40(8), 2908-12, Aug 2002 Abstract
  • Chen YC, Eisner JD, Kattar MM, Rassoulian-Barrett SL, Lafe K, Bui U, Limaye AP, Cookson BT, Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts, Journal of Clinical Microbiology, 39(11), 4042-51, November 2001 Abstract
  • Cookson BT, Cummings LA, Rassoulian Barrett SL, Bacterial antigens elicit T cell responses via adaptive and transitional immune recognition, Current Opinion in Microbiology, 4(3), 267-73, June 2001 Abstract
  • Kattar MM, Cookson BT, Carlson LD, Stiglich SK, Schwartz MA, Nguyen TT, Daza R, Wallis CK, Yarfitz SL, Coyle MB, Tsukamurella strandjordae sp. nov., a proposed new species causing sepsis, Journal of Clinical Microbiology, 39(4), 1467-76, April 2001 Abstract
  • Barrett SL, Cookson BT, Carlson LC, Bernard KA, Coyle MB, Diversity within reference strains of Corynebacterium matruchotii includes Corynebacterium durum and a novel organism, Journal of Clinical Microbiology, 39(3), 943-8, March 2001 Abstract
  • Cookson BT, Brennan MA, Pro-inflammatory programmed cell death, Trends in Microbiology, 9(3), 113-4, March 2001 Abstract
  • Limaye AP, Connolly PA, Sagar M, Fritsche TR, Cookson BT, Wheat LJ, Stamm WE, Transmission of Histoplasma capsulatum by organ transplantation, New England Journal of Medicine, 343(16), 1163-6, October 2000 Abstract
  • Brennan MA, Cookson BT, Salmonella induces macrophage death by caspase-1-dependent necrosis, Molecular Microbiology, 38(1), 31-40, October 2000 Abstract
  • Chen YC, Eisner JD, Kattar MM, Rassoulian-Barrett SL, LaFe K, Yarfitz SL, Limaye AP, Cookson BT, Identification of medically important yeasts using PCR-based detection of DNA sequence polymorphisms in the internal transcribed spacer 2 region of the rRNA genes, Journal of Clinical Microbiology, 38(6), 2302-10, June 2000 Abstract
  • Limaye AP, Turgeon DK, Cookson BT, Fritsche TR, Pseudomembranous colitis caused by a toxin A(-) B(+) strain of Clostridium difficile, Journal of Clinical Microbiology, 38(4), 1696-7, April 2000 Abstract
  • Kattar MM, Chavez JF, Limaye AP, Rassoulian-Barrett SL, Yarfitz SL, Carlson LC, Houze Y, Swanzy S, Wood BL, Cookson BT, Application of 16S rRNA gene sequencing to identify Bordetella hinzii as the causative agent of fatal septicemia, Journal of Clinical Microbiology, 38(2), 789-94, February 2000 Abstract
  • McSorley SJ, Cookson BT, Jenkins MK, Characterization of CD4 T cell responses during natural infection with Salmonella typhimurium, Journal of Immunology, 164(2), 986-93, Jan 2000 Abstract
  • Cookson B T, Bevan M J, Identification of a natural T cell epitope presented by Salmonella-infected macrophages and recognized by T cells from orally immunized mice, Journal of Immunology, 158(9), 4310-9, 1 May 1997 Abstract
  • Mandel L P, Schaad D C, Cookson B T, Curtis J D, Orkand A R, Wener M H, Lecrone C N, Dewitt D, Astion M L, Evaluation of an interactive computer program to teach gram-stain interpretation, Academic Medicine, 71(10 Suppl), S100-2, October 1996 Abstract
  • Vandamme P, Hommez J, Vancanneyt M, Monsieurs M, Hoste B, Cookson B, Wirsing von Konig C H, Kersters K, Blackall P J, Bordetella hinzii sp. nov., isolated from poultry and humans, International Journal of Systematic Bacteriology, 45(1), 37-45, January 1995 Abstract
  • Cookson BT, Vandamme P, Carlson LC, Larson AM, Sheffield JV, Kersters K, Spach DH, Bacteremia caused by a novel Bordetella species, 'B. hinzii', Journal of Clinical Microbiology, 32(10), 2569-71, October 1994 Abstract
  • Cookson B T, Engelhardt S, Smith C, Bamford H A, Prochazka M, Tait J F, Organization of the human annexin V (ANX5) gene, Genomics, 20(3), 463-7, April 1994 Abstract
  • Tait J F, Smith C, Xu L, Cookson B T, Structure and polymorphisms of the human annexin III (ANX3) gene, Genomics, 18(1), 79-86, October 1993 Abstract
  • Cookson BT, Berg DE, Goldman WE, Mutagenesis of Bordetella pertussis with transposon Tn5tac1: conditional expression of virulence-associated genes, Journal of Bacteriology, 172(4), 1681-7, April 1990 Abstract
  • Cookson BT, Cho HL, Herwaldt LA, Goldman WE, Biological activities and chemical composition of purified tracheal cytotoxin of Bordetella pertussis, Infection and Immunity, 57(7), 2223-9, July 1989 Abstract
  • Cookson BT, Tyler AN, Goldman WE, Primary structure of the peptidoglycan-derived tracheal cytotoxin of Bordetella pertussis, Biochemistry, 28(4), 1744-9, February 1989 Abstract
  • Gentry-Weeks CR, Cookson BT, Goldman WE, Rimler RB, Porter SB, Curtiss R 3d, Dermonecrotic toxin and tracheal cytotoxin, putative virulence factors of Bordetella avium, Infection and Immunity, 56(7), 1698-707, July 1988 Abstract
  • Goldman WE, Cookson BT, Structure and functions of the Bordetella tracheal cytotoxin, Tokai Journal of Experimental and Clinical Medicine, 13 Suppl, 187-91, 1988 Abstract
  • Cookson BT, Olivera BM, Roth JR, Genetic characterization and regulation of the nadB locus of Salmonella typhimurium, Journal of Bacteriology, 169(9), 4285-93, September 1987 Abstract
  • Rosenthal RS, Nogami W, Cookson BT, Goldman WE, Folkening WJ, Major fragment of soluble peptidoglycan released from growing Bordetella pertussis is tracheal cytotoxin, Infection and Immunity, 55(9), 2117-20, September 1987 Abstract
  • Hughes KT, Cookson BT, Ladika D, Olivera BM, Roth JR, 6-Aminonicotinamide-resistant mutants of Salmonella typhimurium, Journal of Bacteriology, 154(3), 1126-36, June 1983 Abstract

Profile Details

Last Verified: 9/3/2008

COS Expertise ID #120016
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