Dr. C. S. Raman |
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University of Texas Health Science Center at Houston Medical School Biochemistry and Molecular Biology Assistant ProfessorAppointed: 2000 University of Texas Health Science Center at Houston Medical School Structural Biology Research Center DirectorAppointed: 2002 |  |
Mailing AddressDept. of Biochemistry & Molecular Biology MSB 6.128 6431 Fannin Houston, Texas 77030United States | Contact Information |
Qualifications
Ph.D., University of Texas Health Science Center at San Antonio, Biochemistry and Biophysics, 1996.
Expertise and Research Interests
Cutting edge structural and biological research performed under one roof. We not only solve macromolecular structures using X-ray crystallography, but also utilize prokaryotic, unicellular eukaryote (Chlamydomonas and Saccharomyces), invertebrate (C. elegans), and vertebrate (zebrafish) model sytems to investigate functional correlations.
Ongoing Research Projects:
1. Structural Biology of Nitric Oxide Synthase-like Proteins from Human Pathogens
Funding: NIH, Pew
We are investigating structure-function relationships in the newly discovered nitric oxide synthase-like proteins from Anthrax bacterium and Staphylococcus aureus.
Pierre Nioche (Research Assistant Professor) is the primary contributor to this project.
Collaborators: Jean-Luc Boucher (Paris), Brian Hoffman (Evanston), Theresa Koehler (Houston), Daniel Mansuy (Paris), Pavel Martasek (Prague), Martin Newcomb (Chicago).
2. Substrate and Inhibitor Recognition by Mammalian Nitric Oxide Synthases
Funding: The Robert A. Welch Foundation, NIH/NINDS
This project focuses on crystallographic studies aimed at understading how substrate and inhibitors are selectively recognized by the mammalian nitric oxide synthase machine. In addition we are also investigating the structural networks responsible for electron transfer processes.
Pierre Nioche, Dong-Sun Lee (Research Assistant Professor), and Raegan Hunt (M.D./Ph.D. student) contribute to this project.
Collaborators: Rosa Claramunt (Spain), Jose Elguerro (Spain), Dominique Vichard (Versailles), Francois Terrier (Versailles), Vladimir Kral (Prague), Pavel Martasek (San Antonio), Bettie Sue Siler Masters (San Antonio).
3. Structural Bases of Gaseous Messenger Signal Transduction
Funding: Pew, The Robert A. Welch Foundation
Three decades of research has failed to identify the molecular bases of how gaseous messengers transduce signals to mediate a variety of cellular responses. We have put in place strategies that will both overcome the hurdles experienced by other researchers and provide atomic insights into the mechanisms of signalling by nitric oxide and carbon monoxide.
Pierre Nioche is the primary contributor to this project.
Collaborators: Andrew Camilli (Boston), Peter Ford (Santa Barbara), James Kaper (Baltimore), Nigel Minton (Nottingham) , Michel Negrerie (Paris), John Olson (Houston), Masao-Ikeda Saito (Japan), Takeshi Tomita (Japan), Sudha Veeraraghavan (Houston).
4. Structure-Function Relationships in Heme Biosynthesis
Funding: Pew, The Robert A. Welch Foundation
We study how heme is biosynthesized, transported and degraded in eukaryotes.
Nathan Campbell (Senior Research Assistant) is the primary contributor to this project.
5. Structural Biology of Lipid Mediator Signaling
Funding: Pew, The Robert A. Welch Foundation
We are exploring the enzymes that biosynthesize thromboxane A2 and prostacyclin (PGI2) in humans.
Dong-Sun Lee, Raegan Hunt, & Pierre Nioche contribute to this project.
Collaborators: Volker Ullrich (Konstanz), Didier Stainier
(San Francisco), JD (Belgique), and JMD (Belgique).
6. Novel Regulators of Mitochondrial Homeostasis
Funding: Pew, NIH/NINDS
We have discovered novel proteins that modulate mitochondrial function. Structure-function studies are in progess to elucidate the mechanisms by which this key process is regulated.
This is the dissertation project of Raegan Hunt.
Collaborators: Yurii Chinenov (New York), Pavel Martasek (Prague), Didier Stainier (San Francisco).
7. Structural Bases of Immunodeficiency
Funding: Pew
We are engaged in X-ray crystallographic studies of
Activation-Induced Deaminase (AID).
Dong-Sun Lee is the primary contributor to this project
Collaborators: Vasco Barreto and Michel Nussenzweig (Rockefeller University)
Ongoing Research Projects:
1. Structural Biology of Nitric Oxide Synthase-like Proteins from Human Pathogens
Funding: NIH, Pew
We are investigating structure-function relationships in the newly discovered nitric oxide synthase-like proteins from Anthrax bacterium and Staphylococcus aureus.
Pierre Nioche (Research Assistant Professor) is the primary contributor to this project.
Collaborators: Jean-Luc Boucher (Paris), Brian Hoffman (Evanston), Theresa Koehler (Houston), Daniel Mansuy (Paris), Pavel Martasek (Prague), Martin Newcomb (Chicago).
2. Substrate and Inhibitor Recognition by Mammalian Nitric Oxide Synthases
Funding: The Robert A. Welch Foundation, NIH/NINDS
This project focuses on crystallographic studies aimed at understading how substrate and inhibitors are selectively recognized by the mammalian nitric oxide synthase machine. In addition we are also investigating the structural networks responsible for electron transfer processes.
Pierre Nioche, Dong-Sun Lee (Research Assistant Professor), and Raegan Hunt (M.D./Ph.D. student) contribute to this project.
Collaborators: Rosa Claramunt (Spain), Jose Elguerro (Spain), Dominique Vichard (Versailles), Francois Terrier (Versailles), Vladimir Kral (Prague), Pavel Martasek (San Antonio), Bettie Sue Siler Masters (San Antonio).
3. Structural Bases of Gaseous Messenger Signal Transduction
Funding: Pew, The Robert A. Welch Foundation
Three decades of research has failed to identify the molecular bases of how gaseous messengers transduce signals to mediate a variety of cellular responses. We have put in place strategies that will both overcome the hurdles experienced by other researchers and provide atomic insights into the mechanisms of signalling by nitric oxide and carbon monoxide.
Pierre Nioche is the primary contributor to this project.
Collaborators: Andrew Camilli (Boston), Peter Ford (Santa Barbara), James Kaper (Baltimore), Nigel Minton (Nottingham) , Michel Negrerie (Paris), John Olson (Houston), Masao-Ikeda Saito (Japan), Takeshi Tomita (Japan), Sudha Veeraraghavan (Houston).
4. Structure-Function Relationships in Heme Biosynthesis
Funding: Pew, The Robert A. Welch Foundation
We study how heme is biosynthesized, transported and degraded in eukaryotes.
Nathan Campbell (Senior Research Assistant) is the primary contributor to this project.
5. Structural Biology of Lipid Mediator Signaling
Funding: Pew, The Robert A. Welch Foundation
We are exploring the enzymes that biosynthesize thromboxane A2 and prostacyclin (PGI2) in humans.
Dong-Sun Lee, Raegan Hunt, & Pierre Nioche contribute to this project.
Collaborators: Volker Ullrich (Konstanz), Didier Stainier
(San Francisco), JD (Belgique), and JMD (Belgique).
6. Novel Regulators of Mitochondrial Homeostasis
Funding: Pew, NIH/NINDS
We have discovered novel proteins that modulate mitochondrial function. Structure-function studies are in progess to elucidate the mechanisms by which this key process is regulated.
This is the dissertation project of Raegan Hunt.
Collaborators: Yurii Chinenov (New York), Pavel Martasek (Prague), Didier Stainier (San Francisco).
7. Structural Bases of Immunodeficiency
Funding: Pew
We are engaged in X-ray crystallographic studies of
Activation-Induced Deaminase (AID).
Dong-Sun Lee is the primary contributor to this project
Collaborators: Vasco Barreto and Michel Nussenzweig (Rockefeller University)
Other Expertise
Protein Folding
Bioinformatics
Bioinformatics
Future Research
Structural Biology of Kinetochores
Systems Biology in Isolation
Design of Novel Biosensors
Systems Biology in Isolation
Design of Novel Biosensors
Industrial Relevance
Structure-based Drug Design
Food Biochemistry
Food Biochemistry
Keywords
COS Keywords:
Biochemistry, Bioinformatics, Biophysics, Cardiovascular Diseases, Crystallography, Molecular Biology, Protein Folding, Proteins and Macromolecules, Signal Transduction, Structural Biology.Additional Terms:
Cardiovascular, Gaseous Messengers, Heme Biosynthesis, Nitric Oxide, Protein Crystallography, Signal Transduction, Structural Biology, Systems Biology, X-ray Diffraction.Languages
(Reading, Writing, Speaking)
French: (Functional, Functional, Basic)
Memberships
American Association for the Advancement of Science
American Crystallographic Association
American Physical Society
American Society for Biochemistry and Molecular Biology
Honors and Awards
2005, Co-chair Elect,
Gordon Research Conference - 2007,
Oxidative Stress and Disease
2002, Young Investigator Award,
Nitric Oxide Society
2002-2006,
Pew Scholar Award,
Pew Charitable Trusts
1998-2000,
Dorothy Penrose Stout Fellow,
American Heart Association
Previous Positions
1997-2000, American Heart Association Postdoctoral Fellow,
University of California, Irvine,
Program in Macromolecular Structure
Patents
Structural Basis of Pterin Recognition by Nitric Oxide Synthase,
Patent Number: ,
,
,
United States.
Funding Received
- Robert A. Welch Foundation: Principal Investigator, , 2005 to 2008.
- NIH / NIAID: Co-Investigator, , 2005 to 2009.
- NIH / NIAID - NRSA: Mentor, , 2005 to 2010.
- National Institute of Allergy and Infectious Diseases (NIH): Principal Investigator, , 2003 to 2008.
- NIH / NINDS - NRSA: Mentor, , 2003 to 2007.
- Pew Charitable Trusts: Scholar, , 2002 to 2006.
Publications
- Milewicz DM, Dietz HC, Miller DC, Treatment of Aortic Disease in Patients With Marfan Syndrome., Circulation, 111(11), e150-7, Mar 2005
- Lee DS, Flachsova E, Bodnarova M, Demeler B, Martasek P, Raman CS, Structural Basis of Hereditary Coproporphyria, Proceedings of the National Academy of Sciences USA, 102(40), 14232-14237, 2005
- Nioche P, Berka V, Vipond J, Minton N, Tsai AL, Raman CS, Femtomolar sensitivity of a NO sensor from Clostridium botulinum, Science, 306(5701), 1550-3, Nov 2004
- Kurokawa H, Lee DS, Watanabe M, Sagami I, Mikami B, Raman CS, Shimizu T, A redox-controlled molecular switch revealed by the crystal structure of a
bacterial heme PAS sensor, The Journal of Biological Chemistry, 279(19), 20186-93, May 2004
- Kotsonis P, Frohlich LG, Raman CS, Li H, Berg M, Gerwig R, Groehn V, Kang Y, Al-Masoudi N, Taghavi-Moghadam S, Mohr D, Munch U, Schnabel J, Martasek P, Masters BS, Strobel H, Poulos T, Matter H, Pfleiderer W, Schmidt HH, Structural basis for pterin antagonism in nitric-oxide synthase.
Development of novel 4-oxo-pteridine antagonists of
(6R)-5,6,7,8-tetrahydrobiopterin, Journal of Biological Chemistry, 276(52), 49133-41, December 2001
- Raman CS, Li H, Martasek P, Southan G, Masters BS, Poulos TL, Crystal structure of nitric oxide synthase bound to nitro indazole reveals
a novel inactivation mechanism, Biochemistry, 40(45), 13448-55, November 2001
- Raman CS, Li H, Martasek P, Babu BR, Griffith OW, Masters BS, Poulos TL, Implications for isoform-selective inhibitor design derived from the
binding mode of bulky isothioureas to the heme domain of endothelial
nitric-oxide synthase, Journal of Biological Chemistry, 276(28), 26486-91, July 2001
- Li H, Raman CS, Martasek P, Masters BS, Poulos TL, Crystallographic studies on endothelial nitric oxide synthase complexed
with nitric oxide and mechanism-based inhibitors, Biochemistry, 40(18), 5399-406, May 2001
- Li H, Raman CS, Martasek P, Kral V, Masters BS, Poulos TL, Mapping the active site polarity in structures of endothelial nitric oxide
synthase heme domain complexed with isothioureas, Journal of Inorganic Biochemistry, 81(3), 133-9, August 2000
- Raman CS, Jemmerson R, Nall BT, Antibody-detected folding: kinetics of surface epitope formation are
distinct from other folding phases, Protein Science : a Publication of the Protein Society, 9(1), 129-37, Jan 2000
- Souza JM, Daikhin E, Yudkoff M, Raman CS, Ischiropoulos H, Factors determining the selectivity of protein tyrosine nitration, Archives of Biochemistry and Biophysics, 371(2), 169-78, November 1999
- Pierce MM, Raman CS, Nall BT, Isothermal Titration Calorimetry of Protein-protein Interactions, Methods (San Diego, Calif.), 19(2), 213-21, October 1999
- Li H, Raman CS, Glaser CB, Blasko E, Young TA, Parkinson JF, Whitlow M, Poulos TL, Crystal structures of zinc-free and -bound heme domain of human inducible
nitric-oxide synthase. Implications for dimer stability and comparison
with endothelial nitric-oxide synthase, Journal of Biological Chemistry, 274(30), 21276-84, July 1999
- Miller RT, Martasek P, Raman CS, Masters BS, Zinc content of Escherichia coli-expressed constitutive isoforms of
nitric-oxide synthase. Enzymatic activity and effect of
pterin, Journal of Biological Chemistry, 274(21), 14537-40, May 1999
- Poulos TL, Li H, Raman CS, Heme-mediated oxygen activation in biology: cytochrome c oxidase and
nitric oxide synthase, Current Opinion in Chemical Biology, 3(2), 131-7, April 1999
- Raman CS, Li H, Martasek P, Kral V, Masters BS, Poulos TL, Crystal structure of constitutive endothelial nitric oxide synthase: a
paradigm for pterin function involving a novel metal
center, Cell, 95(7), 939-50, December 1998
- Martasek P, Miller RT, Liu Q, Roman LJ, Salerno JC, Migita CT, Raman CS, Gross SS, Ikeda-Saito M, Masters BS, The C331A mutant of neuronal nitric-oxide synthase is defective in
arginine binding, Journal of Biological Chemistry, 273(52), 34799-805, December 1998
- Poulos TL, Raman CS, Li H, NO News Is Good News, Structure (London, England), 6(3), 255-8, March 1998
- Raaphorst FM, Raman CS, Tami J, Fischbach M, Sanz I, Human Ig heavy chain CDR3 regions in adult bone marrow pre-B cells display
an adult phenotype of diversity: evidence for structural selection of DH
amino acid sequences, International Immunology, 9(10), 1503-15, October 1997
- Martasek P, Camadro JM, Raman CS, Lecomte MC, Le Caer JP, Demeler B, Grandchamp B, Labbe P, Human coproporphyrinogen oxidase. Biochemical characterization of
recombinant normal and R231W mutated enzymes expressed in E. coli as
soluble, catalytically active homodimers, Cellular and Molecular Biology (noisy-le-grand, France), 43(1), 47-58, February 1997
- Raaphorst FM, Raman CS, Nall BT, Teale JM, Molecular mechanisms governing reading frame choice of immunoglobulin
diversity genes, Immunology Today, 18(1), 37-43, January 1997
- Raman CS, Allen MJ, Nall BT, Enthalpy of antibody--cytochrome c binding, Biochemistry, 34(17), 5831-8, May 1995
- Gorovits B, Raman CS, Horowitz PM, High hydrostatic pressure induces the dissociation of cpn60 tetradecamers
and reveals a plasticity of the monomers, Journal of Biological Chemistry, 270(5), 2061-6, February 1995
- Raman CS, Jemmerson R, Nall BT, Allen MJ, Diffusion-limited rates for monoclonal antibody binding to cytochrome c, Biochemistry, 31(42), 10370-9, October 1992
Profile Details
Last Updated: 2/5/2006
COS Expertise ID #1065971
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