Prof. Harry A. Dailey

powered by
COS Expertise®
University of Georgia
Biomedical and Health Sciences Institute
DirectorAppointed: 2001
University of Georgia
College of Arts and Sciences
Biochemistry and Molecular Biology
ProfessorAppointed: 1996
University of Georgia
College of Arts and Sciences
Microbiology
ProfessorAppointed: 1990

Mailing Address

University of Georgia
Biomedical and Health Sciences Institute
N230 Paul D. Coverdell Center for Biomedical and Health Sciences
Athens, Georgia 30602-2779
United States

Contact Information

Phone: (706) 542-2690
Fax: (706) 542-7567
hdailey@uga.edu

Qualifications

Ph.D., University of California, Los Angeles, Microbiology, 1976.
B.A., University of California, Los Angeles, Bacteriology, 1972.

Expertise and Research Interests

Expertise includes:
Heme biosynthesis
Iron metabolism
Protein structure/function
Regulation of heme biosynthesis
Disorders of heme biosynthesis (porphyrias)
Protein expression and purification
Protein chemistry
Membrane protein
Mammalian cell culture

Keywords

COS Keywords:

Biochemistry, Biochemistry, Proteins, Biosynthesis, Cell Components, Cell Culture, Microbiology, Molecular Biology, Protein Structure, Vitamins Or Metabolic Role.

Additional Terms:

Heme Biosynthesis, Iron Metabolism, Mammalian Cell Culture, Protein Chemistry, Regualtion of Heme Biosynthesis.

Memberships

American Porphyria Foundation
American Society for Biochemistry and Molecular Biology
American Society for Microbiology
American Society of Hematology
International BioIron Society
Society of Porphyrins and Phthalocyanines
Tetrapyrrole Discussion Group

Honors and Awards

2000, Merrin Scholar, University of Cape Town
1982-1987, Research Career Development Award, NIDDK, University of Georgia
1972, University of California Presidents Undergraduate Fellowship, University of California, UCLA
1972, American Heart Association Postdoctoral Fellowship, American Heart Association, University of Connecticut Health Center
1971, Summer Undergraduate Research Program, NSF, UCLA

Previous Positions

1990-1996, Head, University of Georgia, College of Arts and Sciences, Microbiology
1987-1989, University of Georgia, Acting Department Head
1986-1987, University of Georgia, Assistant Department Head
1985-1990, University of Georgia, Associate Professor
1984-1987, University of Georgia, Graduate Coordinator
1980-1985, University of Georgia, Assistant Professor
1976-1980, University of Connecticut Health Center, Postdoctoral Assistant

Publications

  • Najahi-Missaoui W, Dailey HA (Aug 2005) Production and characterization of erythropoietic protoporphyric heterodimeric ferrochelatases., Blood, 106 (3), 1098-104 Abstract
  • Dailey TA, Woodruff JH, Dailey HA (Mar 2005) Examination of mitochondrial protein targeting of haem synthetic enzymes: in vivo identification of three functional haem-responsive motifs in 5-aminolaevulinate synthase., The Biochemical Journal, 386 (Pt 2), 381-6 Abstract
  • Whatley SD, Mason NG, Khan M, Zamiri M, Badminton MN, Missaoui WN, Dailey TA, Dailey HA, Douglas WS, Wainwright NJ, Elder GH, Autosomal recessive erythropoietic protoporphyria in the United Kingdom: prevalence and relationship to liver disease, Journal of Medical Genetics, 41(8), e105, Aug 2004 Abstract
  • Mayer MR, Dailey TA, Baucom CM, Supernak JL, Grady MC, Hawk HE, Dailey HA, Expression of human proteins at the Southeast Collaboratory for Structural Genomics, Journal of Structural and Functional Genomics, 5(1-2), 159-65, 2004 Abstract
  • Adams MW, Dailey HA, DeLucas LJ, Luo M, Prestegard JH, Rose JP, Wang BC, The Southeast Collaboratory for Structural Genomics: a high-throughput gene to structure factory, Accounts of Chemical Research, 36(3), 191-8, March 2003 Abstract
  • Jacob Blackmon B, Dailey TA, Lianchun X, Dailey HA, Characterization of a human and mouse tetrapyrrole-binding protein, Archives of Biochemistry and Biophysics, 407(2), 196-201, November 2002 Abstract
  • Dailey HA, Terminal steps of haem biosynthesis, Biochemical Society Transactions, 30(4), 590-5, Aug 2002 Abstract
  • Dailey TA, Dailey HA, Identification of [2Fe-2S] Clusters in Microbial Ferrochelatases, Journal of Bacteriology, 184(9), 2460-4, May 2002 Abstract
  • Dailey TA, McManus JF, Dailey HA, Characterization of the mouse protoporphyrinogen oxidase gene, Cellular and Molecular Biology (noisy-le-grand, France), 48(1), 61-9, February 2002 Abstract
  • Medlock AE, Meissner PN, Davidson BP, Corrigall AV, Dailey HA, A mouse model for South African (R59W) variegate porphyria: construction and initial characterization, Cellular and Molecular Biology (noisy-le-grand, France), 48(1), 71-8, February 2002 Abstract
  • Schubot FD, Chen CJ, Rose JP, Dailey TA, Dailey HA, Wang BC, Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription, Protein Science : a Publication of the Protein Society, 10(10), 1980-8, October 2001 Abstract
  • Wang KF, Dailey TA, Dailey HA, Expression and characterization of the terminal heme synthetic enzymes from the hyperthermophile Aquifex aeolicus, Fems Microbiology Letters, 202(1), 115-9, August 2001 Abstract
  • Sellers VM, Wu CK, Dailey TA, Dailey HA, Human ferrochelatase: characterization of substrate-iron binding and proton-abstracting residues, Biochemistry, 40(33), 9821-7, August 2001 Abstract
  • Wu CK, Dailey HA, Rose JP, Burden A, Sellers VM, Wang BC, The 2.0 A structure of human ferrochelatase, the terminal enzyme of heme biosynthesis, Nature Structural Biology, 8(2), 156-60, February 2001 Abstract
  • Woodard SI, Dailey HA, Multiple regulatory steps in erythroid heme biosynthesis, Archives of Biochemistry and Biophysics, 384(2), 375-8, 2000 Abstract
  • Dailey HA, Dailey TA, Wu CK, Medlock AE, Wang KF, Rose JP, Wang BC, Ferrochelatase at the millennium: structures, mechanisms and [2Fe-2S] clusters, Cellular and Molecular Life Sciences, 57(13-14), 1909-26, December 2000 Abstract
  • Gamble JT, Wong SG, Dailey HA, Marks GS, Use of recombinant human ferrochelatase as a sensitive bioassay for N-alkylprotoporphyrin IX formed after interaction of porphyrinogenic xenobiotics with rat liver microsomes, Canadian Journal of Physiology and Pharmacology, 78(7), 578-81, July 2000 Abstract
  • Medlock AE, Dailey HA, Examination of the activity of carboxyl-terminal chimeric constructs of human and yeast ferrochelatases, Biochemistry, 39(25), 7461-7, 2000 Abstract
  • Gamble JT, Dailey HA, Marks GS, N-Methylprotoporphyrin is a more potent inhibitor of recombinant human than of recombinant chicken ferrochelatase, Drug Metabolism and Disposition, 28(4), 373-5, April 2000 Abstract
  • Burden AE, Wu C, Dailey TA, Busch JL, Dhawan IK, Rose JP, Wang B, Dailey HA, Human ferrochelatase: crystallization, characterization of the [2Fe-2S] cluster and determination that the enzyme is a homodimer., Biochimica et Biophysica Acta, 1435(1-2), 191-7, 16 Nov 1999 Abstract
  • Wang KF, Wu CK, Sellers VM, Rose JP, Wang BC, Dailey HA, Purification, crystallization and preliminary X-ray analysis of Drosophila melanogaster ferrochelatase., Acta Crystallographica, Section D: Biological Crystallography, 55 \( Pt 6\), 1201-3, June 1999 Abstract
  • Day AL, Parsons BM, Dailey HA, Cloning and characterization of Gallus and Xenopus ferrochelatases: presence of the [2Fe-2S] cluster in nonmammalian ferrochelatase, Archives of Biochemistry and Biophysics, 359(2), 160-9, 1998 Abstract
  • Roberts AG, Puy H, Dailey TA, Morgan RR, Whatley SD, Dailey HA, Martasek P, Nordmann Y, Deybach JC, Elder GH, Molecular characterization of homozygous variegate porphyria, Human Molecular Genetics, 7(12), 1921-5, November 1998 Abstract
  • Corrigall AV, Siziba KB, Maneli MH, Shephard EG, Ziman M, Dailey TA, Dailey HA, Kirsch RE, Meissner PN, Purification of and kinetic studies on a cloned protoporphyrinogen oxidase from the aerobic bacterium Bacillus subtilis, Archives of Biochemistry and Biophysics, 358(2), 251-6, 1998 Abstract
  • Sellers V M, Wang K F, Johnson M K, Dailey H A, Evidence that the fourth ligand to the [2Fe-2S] cluster in animal ferrochelatase is a cysteine. Characterization of the enzyme from Drosophila melanogaster., Journal of Biological Chemistry, 273(35), 22311-6, 28 Aug 1998 Abstract
  • Dailey T A, Dailey H A, Identification of an FAD superfamily containing protoporphyrinogen oxidases, monoamine oxidases, and phytoene desaturase. Expression and characterization of phytoene desaturase of Myxococcus xanthus., Journal of Biological Chemistry, 273(22), 13658-62, 29 May 1998 Abstract
  • Sellers V M, Dailey T A, Dailey H A, Examination of ferrochelatase mutations that cause erythropoietic protoporphyria., Blood, 91(10), 3980-5, 15 May 1998 Abstract
  • Yin X, Dailey H A, Erythroid 5-aminolevulinate synthase is required for erythroid differentiation in mouse embryonic stem cells., Blood Cells, Molecules, and Diseases, 24(1), 41-53, March 1998 Abstract
  • Hift R J, Meissner P N, Corrigall A V, Ziman M R, Petersen L A, Meissner D M, Davidson B P, Sutherland J, Dailey H A, Kirsch R E, Variegate porphyria in South Africa, 1688-1996--new developments in an old disease., South African Medical Journal, 87(6), 722-31, June 1997 Abstract
  • Dailey H A, Dailey T A, Characteristics of human protoporphyrinogen oxidase in controls and variegate porphyrias., Cellular and Molecular Biology, 43(1), 67-73, February 1997 Abstract
  • Dailey H A, Dailey T A, Expression and purification of mammalian 5-aminolevulinate synthase., Methods In Enzymology, 281, 336-40, 1997 Abstract
  • Sellers V M, Dailey H A, Expression, purification, and characterization of recombinant mammalian ferrochelatase., Methods In Enzymology, 281, 378-87, 1997 Abstract
  • Dailey T A, Dailey H A, Expression, purification, and characteristics of mammalian protoporphyrinogen oxidase., Methods In Enzymology, 281, 340-9, 1997 Abstract
  • Medlock A E, Dailey H A, Human coproporphyrinogen oxidase is not a metalloprotein., Journal of Biological Chemistry, 271(51), 32507-10, 20 Dec 1996 Abstract
  • Crouse B R, Sellers V M, Finnegan M G, Dailey H A, Johnson M K, Site-directed mutagenesis and spectroscopic characterization of human ferrochelatase: identification of residues coordinating the [2Fe-2S] cluster., Biochemistry, 35(50), 16222-9, 17 Dec 1996 Abstract
  • Meissner P N, Dailey T A, Hift R J, Ziman M, Corrigall A V, Roberts A G, Meissner D M, Kirsch R E, Dailey H A, A R59W mutation in human protoporphyrinogen oxidase results in decreased enzyme activity and is prevalent in South Africans with variegate porphyria [see comments], Nature Genetics, 13(1), 95-7, May 1996 Abstract
  • Dailey H A, Dailey T A, Protoporphyrinogen oxidase of Myxococcus xanthus. Expression, purification, and characterization of the cloned enzyme., Journal of Biological Chemistry, 271(15), 8714-8, 12 Apr 1996 Abstract
  • Sellers V M, Johnson M K, Dailey H A, Function of the [2FE-2S] cluster in mammalian ferrochelatase: a possible role as a nitric oxide sensor., Biochemistry, 35(8), 2699-704, 27 Feb 1996 Abstract
  • Dailey T A, Dailey H A, Human protoporphyrinogen oxidase: expression, purification, and characterization of the cloned enzyme., Protein Science, 5(1), 98-105, January 1996 Abstract
  • Dailey T A, Dailey H A, Meissner P, Prasad A R, Cloning, sequence, and expression of mouse protoporphyrinogen oxidase., Archives of Biochemistry and Biophysics, 324(2), 379-84, 20 Dec 1995 Abstract
  • Prasad A R, Dailey H A, Generation of resistance to the diphenyl ether herbicide acifluorfen by MEL cells., Biochemical and Biophysical Research Communications, 215(1), 186-91, 4 Oct 1995 Abstract
  • Prasad A R, Dailey H A, Effect of cellular location on the function of ferrochelatase., Journal of Biological Chemistry, 270(31), 18198-200, 4 Aug 1995 Abstract
  • Prades E, Chambon C, Dailey T A, Dailey H A, Briere J, Grandchamp B, A new mutation of the ALAS2 gene in a large family with X-linked sideroblastic anemia., Human Genetics, 95(4), 424-8, April 1995 Abstract
  • Woodard S I, Dailey H A, Regulation of heme biosynthesis in Escherichia coli., Archives of Biochemistry and Biophysics, 316(1), 110-5, 10 Jan 1995 Abstract
  • Dailey H A, Finnegan M G, Johnson M K, Human ferrochelatase is an iron-sulfur protein., Biochemistry, 33(2), 403-7, 18 Jan 1994 Abstract
  • Dailey T A, Meissner P, Dailey H A, Expression of a cloned protoporphyrinogen oxidase., Journal of Biological Chemistry, 269(2), 813-5, 14 Jan 1994 Abstract
  • Dailey H A, Sellers V M, Dailey T A, Mammalian ferrochelatase. Expression and characterization of normal and two human protoporphyric ferrochelatases., Journal of Biological Chemistry, 269(1), 390-5, 7 Jan 1994 Abstract
  • Dailey, H.A., Sellers, V.M., and Dailey, T.A., Mammalian ferrochelatase. Expression and characterization of normal and two human protoporphyric ferrochelatases, J. Biol. Chem., 269, in press, 1994
  • Lake-Bullock H, Dailey H A, Biphasic ordered induction of heme synthesis in differentiating murine erythroleukemia cells: role of erythroid 5-aminolevulinate synthase., Molecular and Cellular Biology, 13(11), 7122-32, November 1993 Abstract
  • Proulx K L, Woodard S I, Dailey H A, In situ conversion of coproporphyrinogen to heme by murine mitochondria: terminal steps of the heme biosynthetic pathway., Protein Science, 2(7), 1092-8, July 1993 Abstract
  • Boulechfar S, Lamoril J, Montagutelli X, Guenet J L, Deybach J C, Nordmann Y, Dailey H, Grandchamp B, de Verneuil H, Ferrochelatase structural mutant (Fechm1Pas) in the house mouse., Genomics, 16(3), 645-8, June 1993 Abstract
  • Ferreira, G.C., and Dailey, H.A., Expression of mammalian 5-aminolevulinate synthase in Escherichia coli: Overproduction, purification and characterization, J. Biol. Chem., 286, 584-90, 1993
  • Prouix, K.L., Woodard, S.I., and Dailey, H.A., In situ conversion of coproporphyrinogen to heme by murine mitochondria. Terminal steps of the heme biosynthetic pathway, Prot. Sci., 2, 1092-8, 1993
  • Boulechfar, S., Lamoril, J., Montagutelli, X., Guenet, J.-L., Deybach, J.C., Nordmann, Y., Dailey, H.A., Grandchamp, B., and De Verneuil, H., Ferrochelatase structural mutant (Fech m1Pas) in the house mouse, Genomics, 16, 645-8, 1993
  • Lake-Bullock, H., and Dailey, H.A., Biphasic ordered induction of heme synthesis in differentiating murine erythroleukemia cells: Role of erythroid 5-aminolevulinate synthase, Mol. Cell. Biol., 13, in press, 1993
  • Proulx K L, Dailey H A, Characteristics of murine protoporphyrinogen oxidase., Protein Science, 1(6), 801-9, June 1992 Abstract
  • Eldridge M G, Dailey H A, Serum-free, defined medium for the growth and differentiation of murine erythroleukemia cells., Biotechniques, 12(6), 848-53, June 1992 Abstract
  • Brenner DA, Didier JM, Frasier F, Christensen SR, Evans GA, Dailey HA, A molecular defect in human protoporphyria, American Journal of Human Genetics, 50(6), 1203-10, June 1992 Abstract
  • Eldridge M G, Dailey H A, Yeast ferrochelatase: expression in a baculovirus system and purification of the expression protein., Protein Science, 1(2), 271-7, February 1992 Abstract
  • Conder L H, Woodard S I, Dailey H A, Multiple mechanisms for the regulation of haem synthesis during erythroid cell differentiation. Possible role for coproporphyrinogen oxidase., Biochemical Journal, 275 ( Pt 2), 321-6, 15 Apr 1991 Abstract
  • Dailey HA, Jones CS, Karr SW, Interaction of free porphyrins and metalloporphyrins with mouse ferrochelatase. A model for the active site of ferrochelatase, Biochimica Et Biophysica Acta, 999(1), 7-11, 1989 Abstract
  • Karr SR, Dailey HA, The synthesis of murine ferrochelatase in vitro and in vivo, Biochemical Journal, 254(3), 799-803, 1988 Abstract
  • Ferreira GC, Andrew TL, Karr SW, Dailey HA, Organization of the terminal two enzymes of the heme biosynthetic pathway. Orientation of protoporphyrinogen oxidase and evidence for a membrane complex, Journal of Biological Chemistry, 263(8), 3835-9, 1988 Abstract
  • Fadigan A, Dailey HA, Inhibition of ferrochelatase during differentiation of murine erythroleukaemia cells, Biochemical Journal, 243(2), 419-24, 1987 Abstract
  • Dailey HA, Metal inhibition of ferrochelatase, Annals of the New York Academy of Sciences, 514, 81-6, 1987 Abstract
  • Dailey HA, Fleming JE, The role of arginyl residues in porphyrin binding to ferrochelatase, Journal of Biological Chemistry, 261(17), 7902-5, 1986 Abstract
  • Dailey HA, Purification and characterization of bacterial ferrochelatase, Methods in Enzymology, 123, 408-15, 1986 Abstract
  • Dailey HA, Fleming JE, Harbin BM, Purification and characterization of mammalian and chicken ferrochelatase, Methods in Enzymology, 123, 401-8, 1986 Abstract
  • Dailey HA, Fleming JE, Harbin BM, Ferrochelatase from Rhodopseudomonas sphaeroides: substrate specificity and role of sulfhydryl and arginyl residues, Journal of Bacteriology, 165(1), 1-5, January 1986 Abstract
  • Moody MD, Dailey HA, Ferric iron reductase of Rhodopseudomonas sphaeroides, Journal of Bacteriology, 163(3), 1120-5, September 1985 Abstract
  • Dailey HA, Spectroscopic examination of the active site of bovine ferrochelatase, Biochemistry, 24(6), 1287-91, 1985 Abstract
  • Harbin BM, Dailey HA, Orientation of ferrochelatase in bovine liver mitochondria, Biochemistry, 24(2), 366-70, 1985 Abstract
  • Dailey HA, Smith A, Differential interaction of porphyrins used in photoradiation therapy with ferrochelatase, Biochemical Journal, 223(2), 441-5, 1984 Abstract
  • Moody MD, Dailey HA, Siderophore utilization and iron uptake by Rhodopseudomonas sphaeroides, Archives of Biochemistry and Biophysics, 234(1), 178-86, October 1984 Abstract
  • Hanson JW, Dailey HA, Purification and characterization of chicken erythrocyte ferrochelatase, Biochemical Journal, 222(3), 695-700, 1984 Abstract
  • Dailey HA, Effect of sulfhydryl group modification on the activity of bovine ferrochelatase, Journal of Biological Chemistry, 259(5), 2711-5, 1984 Abstract
  • Dailey HA, Fleming JE, Bovine ferrochelatase. Kinetic analysis of inhibition by N-methylprotoporphyrin, manganese, and heme, Journal of Biological Chemistry, 258(19), 11453-9, 1983 Abstract
  • Moody MD, Dailey HA, Aerobic ferrisiderophore reductase assay and activity stain for native polyacrylamide gels, Analytical Biochemistry, 134(1), 235-9, 1983 Abstract
  • Dailey HA, Purification and characterization of membrane-bound ferrochelatase from Rhodopseudomonas sphaeroides, Journal of Biological Chemistry, 257(24), 14714-8, 1982 Abstract
  • Dailey HA, Strittmatter P, Orientation of the carboxyl and NH2 termini of the membrane-binding segment of cytochrome b5 on the same side of phospholipid bilayers, Journal of Biological Chemistry, 256(8), 3951-5, 1981 Abstract
  • Dailey HA, Strittmatter P, The role of COOH-terminal anionic residues in binding cytochrome b5 to phospholipid vesicles and biological membranes, Journal of Biological Chemistry, 256(4), 1677-80, 1981 Abstract
  • Dailey HA, Strittmatter P, Characterization of the interaction of amphipathic cytochrome b5 with stearyl coenzyme A desaturase and NADPH:cytochrome P-450 reductase, Journal of Biological Chemistry, 255(11), 5184-9, 1980 Abstract
  • Dailey HA, Strittmatter P, Modification and identification of cytochrome b5 carboxyl groups involved in protein-protein interaction with cytochrome b5 reductase, Journal of Biological Chemistry, 254(12), 5388-96, 1979 Abstract
  • Dailey HA, Strittmatter P, Structural and functional properties of the membrane binding segment of cytochrome b5, Journal of Biological Chemistry, 253(22), 8203-9, 1978 Abstract
  • Fleming PJ, Dailey HA, Corcoran D, Strittmatter P, The primary structure of the nonpolar segment of bovine cytochrome b5, Journal of Biological Chemistry, 253(15), 5369-72, 1978 Abstract
  • Dailey HA, Purification and characterization of the membrane-bound ferrochelatase from Spirillum itersonii, Journal of Bacteriology, 132(1), 302-7, October 1977 Abstract
  • Dailey HA, Lascelles J, Reduction of iron and synthesis of protoheme by Spirillum itersonii and other organisms, Journal of Bacteriology, 129(2), 815-20, February 1977 Abstract
  • Dailey HA, Lascelles J, Ferrochelatase activity in wild-type and mutant strains of Spirillum itersonii. Solubilization with chaotropic reagents, Archives of Biochemistry and Biophysics, 160(2), 523-9, February 1974 Abstract

Profile Details

Last Updated: 12/18/2006

COS Expertise ID #304030
Reference this profile directly: http://myprofile.cos.com/daileyh30