Dr. Timothy J. Donohue

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University of Wisconsin-Madison
College of Agricultural and Life Sciences
Bacteriology
ProfessorAppointed: 1986
University of Wisconsin-Madison
College of Agricultural and Life Sciences
Great Lakes Bioenergy Research Center
DirectorAppointed: 2007
Professional Headshot of Timothy J. Donohue

Mailing Address

University of Wisconsin-Madison
Room 5159 Microbial Sciences Building
1550 Linden Drive
Madison, Wisconsin 53706
United States

Contact Information

Phone: (608) 262-4663
Fax: (608) 890-2270
tdonohue@bact.wisc.edu
http://www.bact.wisc.edu/faculty.php?init=TJD&show=PEP

Qualifications

Ph.D., Pennsylvania State University, 1980.
M.S., Pennsylvania State University, 1977.

Expertise and Research Interests

Our laboratory analyzes pathways and networks cells use to generate biomass or biofuels from sunlight or other renewable sources of energy. To dissect this fundamentally important problem, we are studying metabolic and regulatory pathways of the photosynthetic bacterium Rhodobacter sphaeroides. By taking advantage of the R. sphaeroides genome sequence, microarrays, proteomics and molecular techniques are being used to determine how the energy in sunlight or renewable nutrients is diverted into processes like cell growth or formation of biofuels.
Specific interests include determining how cells sense the presence of singlet oxygen, a byproduct of solar energy capture during photosynthesis, the role of alternative sigma factors in this response, and the gene products used by cells to repair or prevent the toxic effects of this reactive oxygen species. Approaches taken to solve these problems include biochemical analysis of the interaction of singlet oxygen with purified proteins, computational, genetic, and genome-wide analyses of genes and proteins involved in the response to this reactive oxygen species, and physiological analysis to determine the function of these gene products in the response to singlet oxygen.
The long range goals are to identify metabolic and regulatory activities that are critical to bioenergy formation, to obtain a thorough understanding of energy-generating pathways of agricultural, environmental and medical importance, and to use computational models to help design microbial machines with increased capacity to utilize solar energy, generate renewable sources of energy, remove toxic compounds, or synthesize biodegradable polymers.

Other Expertise

Academic Experience:
- Editorial Board - Journal of Bacteriology, January 1988 - 1999
- Chair -American Society for Microbiology Graduate Student Travel Awards Committee, July, 1991 - June, 1993
- Co-Organizer - Midwest Procaryotic Molecular Genetics and Physiology Club, 1992 - 1999
- Study Section - National Research Council Post-Doctoral Fellowship Program, 1995 - 1997
- Chair-Elect - Genetics and Molecular Biology Divisional Group, American Society for Microbiology (July 1995 - June 1996)
- Selection Committee - American Society for Microbiology Carski Teaching Award (July, 1995 - June 1996)
- Public Communications Committee - American Society for Microbiology (July 1995- Present)
- Director and Principal Investigator - NIH Biotechnology Graduate Training Program at UW-Madison (January 1996 - Present)
- Chair - Genetics and Molecular Biology Divisional Group, American Society for Microbiology (July 1996 - June 1997)
- Chair - American Society for Microbiology Carski Teaching Award (July, 1996 - June 1998)
- Member of the Editorial Board- Microbiology, July 1997 - 2002
- Councilor-at-Large - American Society for Microbiology, July, 1998 - June 2000
- Member - NIGMS Biomedical Research & Institutional Research Training Grants Study Section,July 1999 - Present
- Member of the Editorial Board/Editor - Archives of Microbiology, July 2001 - Present
- Editor - Microbiology, July 2002 - June 2007
- Editor - Archives of Microbiology, July 2002- June 2007
- Editorial Board - Journal of Bacteriology, January 2007 - Present.
- Director, Wisconsin Bioenergy Initiative, University of Wisconsin, June 2007 - April 2009.
- American Academy of Microbiology, Intel Science Award Committee, July 2007 - June 2010.
- Senior Editor, Biofuels, Future Science Group Publishing, UK, March 2009 - Present.

Awards:
- Elected Fellow, American Association for the Advancement of Science, December 2009.
- Burroughs-Wellcome/American Society for Microbiology Visiting Professor of Microbiology, Oakland University, Oakland, MI, March, 2002
- Elected Fellow, American Academy of Microbiology, American Society for Microbiology, October 1999
- University of Wisconsin-Madison, College of Agricultural and Life Sciences Pound Research Excellence Award, 1993
- National Institutes of Health National Research ServiceAward, June 1980 - May 1983
- American Cancer Society Postdoctoral Fellowship, June 1980 (Declined)
- Illinois Heart Association Postdoctoral Fellowship, June 1980 (Declined)
- Alfred E. Sobel prize for outstanding undergraduate research in biochemistry andlife sciences; Polytechnic Institute of Brooklyn, June, 1975

Future Research

We are also using a syatems biology approach to probe featurss of microbes that are of industrial, medical agricultural and environmental significance. In addition, we are using these approaches to assess how variation of genetic traits impacts the production of biofuels from biomass and the function or and communication of individual species in communities or ecosystems.

We have also initiated a new program to determine how cells sense and respond to singlet oxygen, a reactive oxygen species that is a tosci by product of photosynthesis and is used by many eucaryotic cells to defend against unwanated microbial pathogens

Industrial Relevance

Biofuels
Microbial Biotechnology
Metabolic Engineering
Microbial Bioremediation
Microbial Biosensors
Microbial Bioenergy
Synthetic/Systems Microbiology

Keywords

COS Keywords:

Bacteriology, Bioremediation, Biosensors, Gene Expression, Microbial Processes, Photosynthesis, Proteins and Macromolecules, Signal Transduction, Transcription.

Additional Terms:

Autotrophy, Bacterial, Bioenergy, Bioremediation, Biosensors, Electron Transport, Energy Generation, Formaldehyde, Gene Expression, Gene Expression, Hemoproteins, Metalloproteins, Microbial Physiology, One Carbon Metabolism, Photosynthesis, Protein Export, Redox, Rhodobacter Sphaeroides, Rhodospirillaceae, Sigma Factor, Signal Transduction, Stress Response, Transcription.

Memberships

American Academy of Microbiology
American Association for the Advancement of Science
American Chemical Society
American Society for Microbiology
Federation of American Societies for Experimental Biology
Society for General Microbiology
Society for Industrial Microbiology
Society for the Advancement of Chicanos and Native Americans in Science

Previous Positions

1980-1986, University of Illinois, Postdoctoral
1986-1991, University of Wisconsin-Madison, Assistant Professor of Bacteriology
1991-1996, University of Wisconsin-Madison, Associate Professor of Bacteriology

Patents

Singlet oxygen technologies, Patent Number: Pending, 2005, Institution, United States of America.
Microbial System for Formaldehyde Sensing and Remediation, Patent Number: 5747328, 1998, Wisconsin Alumni Research, United States of America.
Microbial System for Formaldehyde Sensing and Remediation, Patent Number: 5834300, 1998, Wisconsin Alumni Research, United States of America.
Microbial System for Formaldehyde Sensing and Remediation, Patent Number: 5837481, 1998, Wisconsin Alumni Research, United States of America.

Funding Received

  • United States Department of Energy (DOE): ARRA Sustainability Initiative for Great Lakes Bioenergy Research Center, 3,022,111, 2010 to 2012.
  • United States Department of Energy (DOE): ARRA Plant Feedstock Initiative for Great Lakes Bioenergy Research Center, 4,099,000, 2009 to 2011.
  • United States Department of Energy (DOE): Great Lakes Bioenergy Research Center, 134,000,000, 2007 to 2012.
  • United States Department of Energy (DOE): Networks Impacting Solar-powered Hydrogen Production, 1,200,000, 2007 to 2010.
  • National Institutes of Health (NIH): Bacterial Response to Singlet Oxygen, 267,187, 2006 to 2010.
  • Department of Energy: Response to Photooxidative Stress, 140,000 per year, 2005 to 2009.

Publications

  • Gomelsky L, Moskvin OV, Stenzel RA, Jones DF, Donohue TJ, Gomelsky M (Dec 2008) Hierarchical regulation of photosynthesis gene expression by the oxygen-responsive PrrBA and AppA-PpsR systems of Rhodobacter sphaeroides., Journal of bacteriology, 190 (24), 8106-14 Abstract
  • Dufour YS, Landick R, Donohue TJ (Nov 2008) Organization and evolution of the biological response to singlet oxygen stress., Journal of molecular biology, 383 (3), 713-30 Abstract
  • Du X, Callister SJ, Manes NP, Adkins JN, Alexandridis RA, Zeng X, Roh JH, Smith WE, Donohue TJ, Kaplan S, Smith RD, Lipton MS (Jul 2008) A computational strategy to analyze label-free temporal bottom-up proteomics data., Journal of proteome research, 7 (7), 2595-604 Abstract
  • Tiedje J, Donohue T (May 2008) Microbes in the energy grid., Science (New York, N.Y.), 320 (5879), 985 Abstract
  • Cho YK, Donohue TJ, Tejedor I, Anderson MA, McMahon KD, Noguera DR (Mar 2008) Development of a solar-powered microbial fuel cell., Journal of applied microbiology, 104 (3), 640-50 Abstract
  • Wilson SM, Gleisten MP, Donohue TJ (Jan 2008) Identification of proteins involved in formaldehyde metabolism by Rhodobacter sphaeroides., Microbiology (Reading, England), 154 (Pt 1), 296-305 Abstract
  • Zeng X, Roh JH, Callister SJ, Tavano CL, Donohue TJ, Lipton MS, Kaplan S (Oct 2007) Proteomic characterization of the Rhodobacter sphaeroides 2.4.1 photosynthetic membrane: identification of new proteins., Journal of bacteriology, 189 (20), 7464-74 Abstract
  • Campbell EA, Greenwell R, Anthony JR, Wang S, Lim L, Das K, Sofia HJ, Donohue TJ, Darst SA (Sep 2007) A conserved structural module regulates transcriptional responses to diverse stress signals in bacteria., Molecular cell, 27 (5), 793-805 Abstract
  • Callister SJ, Nicora CD, Zeng X, Roh JH, Dominguez MA, Tavano CL, Monroe ME, Kaplan S, Donohue TJ, Smith RD, Lipton MS (Dec 2006) Comparison of aerobic and photosynthetic Rhodobacter sphaeroides 2.4.1 proteomes., Journal of microbiological methods, 67 (3), 424-36 Abstract
  • Tavano CL, Donohue TJ (Dec 2006) Development of the bacterial photosynthetic apparatus., Current opinion in microbiology, 9 (6), 625-31 Abstract
  • Donohue TJ, Cogdell RJ (Nov 2006) Microorganisms and clean energy., Nature reviews. Microbiology, 4 (11), 800 Abstract
  • Green HA, Donohue TJ (Aug 2006) Activity of Rhodobacter sphaeroides RpoHII, a second member of the heat shock sigma factor family., Journal of bacteriology, 188 (16), 5712-21 Abstract
  • Callister SJ, Dominguez MA, Nicora CD, Zeng X, Tavano CL, Kaplan S, Donohue TJ, Smith RD, Lipton MS (Aug 2006) Application of the accurate mass and time tag approach to the proteome analysis of sub-cellular fractions obtained from Rhodobacter sphaeroides 2.4.1. Aerobic and photosynthetic cell cultures., Journal of proteome research, 5 (8), 1940-7 Abstract
  • Laguri C, Stenzel RA, Donohue TJ, Phillips-Jones MK, Williamson MP (Jun 2006) Activation of the global gene regulator PrrA (RegA) from Rhodobacter sphaeroides., Biochemistry, 45 (25), 7872-81 Abstract
  • Ranson-Olson B, Jones DF, Donohue TJ, Zeilstra-Ryalls JH (May 2006) In vitro and in vivo analysis of the role of PrrA in Rhodobacter sphaeroides 2.4.1 hemA gene expression., Journal of bacteriology, 188 (9), 3208-18 Abstract
  • Tavano CL, Podevels AM, Donohue TJ (Aug 2005) Identification of genes required for recycling reducing power during photosynthetic growth., Journal of Bacteriology, 187 (15), 5249-58 Abstract
  • Anthony JR, Warczak KL, Donohue TJ, A Transcriptional Response to Singlet Oxygen, a Toxic Byproduct Of Photosynthesis., Proceedings of the National Academy of Sciences of the United States of America., 102(18), 6502-7, May 2005 Abstract
  • Hickman JW, Witthuhn VC Jr, Dominguez M, Donohue TJ, Positive and Negative Transcriptional Regulators of Glutathione-dependent Formaldehyde Metabolism., Journal of Bacteriology, 186(23), 7914-25, Dec 2004 Abstract
  • Donohue TJ, Thomas CM, Policy Proposal for Publication of Papers With Data Sets From Genome-wide Studies., Microbiology (reading, England), 150(Pt 11), 3521-2, Nov 2004 Abstract
  • Anthony JR, Newman JD, Donohue TJ, Interactions Between the Rhodobacter Sphaeroides ECF Sigma Factor, Sigma(E), and Its Anti-sigma Factor, ChrR., Journal of Molecular Biology, 341(2), 345-60, Aug 2004 Abstract
  • Tavano CL, Comolli JC, Donohue TJ, The Role of Dor Gene Products in Controlling the P2 Promoter of The Cytochrome C2 Gene, CycA, in Rhodobacter Sphaeroides., Microbiology (reading, England), 150(Pt 6), 1893-9, Jun 2004 Abstract
  • Comolli JC, Donohue TJ, Differences in Two Pseudomonas Aeruginosa Cbb3 Cytochrome Oxidases., Molecular Microbiology, 51(4), 1193-203, Feb 2004 Abstract
  • Zhou S, Kvikstad E, Kile A, Severin J, Forrest D, Runnheim R, Churas C, Hickman JW, Mackenzie C, Choudhary M, Donohue T, Kaplan S, Schwartz DC (Sep 2003) Whole-genome shotgun optical mapping of Rhodobacter sphaeroides strain 2.4.1 and its use for whole-genome shotgun sequence assembly., Genome research, 13 (9), 2142-51 Abstract
  • Rios-Velazquez C, Coller R, Donohue TJ (Jan 2003) Features of Rhodobacter sphaeroides CcmFH., Journal of bacteriology, 185 (2), 422-31 Abstract
  • Comolli JC, Donohue TJ (Aug 2002) Pseudomonas aeruginosa RoxR, a response regulator related to Rhodobacter sphaeroides PrrA, activates expression of the cyanide-insensitive terminal oxidase., Molecular microbiology, 45 (3), 755-68 Abstract
  • Comolli JC, Carl AJ, Hall C, Donohue T (Jan 2002) Transcriptional activation of the Rhodobacter sphaeroides cytochrome c(2) gene P2 promoter by the response regulator PrrA., Journal of bacteriology, 184 (2), 390-9 Abstract
  • Hickman JW, Barber RD, Skaar EP, Donohue TJ (Jan 2002) Link between the membrane-bound pyridine nucleotide transhydrogenase and glutathione-dependent processes in Rhodobacter sphaeroides., Journal of bacteriology, 184 (2), 400-9 Abstract
  • Newman JD, Anthony JR, Donohue TJ (Oct 2001) The importance of zinc-binding to the function of Rhodobacter sphaeroides ChrR as an anti-sigma factor., Journal of molecular biology, 313 (3), 485-99 Abstract
  • Cox RL, Patterson C, Donohue TJ (Aug 2001) Roles for the Rhodobacter sphaeroides CcmA and CcmG proteins., Journal of bacteriology, 183 (15), 4643-7 Abstract
  • Ríos-Velázquez C, Cox RL, Donohue TJ (May 2001) Characterization of Rhodobacter sphaeroides cytochrome c(2) proteins with altered heme attachment sites., Archives of biochemistry and biophysics, 389 (2), 234-44 Abstract
  • MacKenzie, C., Choudhary, M., Larimer, F. W. Predki, P. F., Stilwagen, S., Armitage, J. P., Barber, R. D., Donohue, T. J., Hosler, J. P., Newman, J., Shapleigh, J. P., Sockett, R. E., Zeilstra-Ryalls, J. and S. Kaplan, The home stretch, a preliminary analysis of the nearly completed genome of Rhodobacter sphaeroides 2.4.1, Photosynthesis Research, 70, :19-41, 2001
  • Karls RK, Wolf JR, Donohue TJ (Nov 1999) Activation of the cycA P2 promoter for the Rhodobacter sphaeroides cytochrome c2 gene by the photosynthesis response regulator., Molecular microbiology, 34 (4), 822-35 Abstract
  • Newman JD, Falkowski MJ, Schilke BA, Anthony LC, Donohue TJ (Nov 1999) The Rhodobacter sphaeroides ECF sigma factor, sigma(E), and the target promoters cycA P3 and rpoE P1., Journal of molecular biology, 294 (2), 307-20 Abstract
  • He Y, Gaal T, Karls R, Donohue TJ, Gourse RL, Roberts GP (Apr 1999) Transcription activation by CooA, the CO-sensing factor from Rhodospirillum rubrum. The interaction between CooA and the C-terminal domain of the alpha subunit of RNA polymerase., The Journal of biological chemistry, 274 (16), 10840-5 Abstract
  • Barber RD, Donohue TJ (Jul 1998) Pathways for transcriptional activation of a glutathione-dependent formaldehyde dehydrogenase gene., Journal of molecular biology, 280 (5), 775-84 Abstract
  • MacGregor BJ, Karls RK, Donohue TJ (Jan 1998) Transcription of the Rhodobacter sphaeroides cycA P1 promoter by alternate RNA polymerase holoenzymes., Journal of bacteriology, 180 (1), 1-9 Abstract
  • Karls RK, Brooks J, Rossmeissl P, Luedke J, Donohue TJ (Jan 1998) Metabolic roles of a Rhodobacter sphaeroides member of the sigma32 family., Journal of bacteriology, 180 (1), 10-9 Abstract
  • Barber RD, Donohue TJ (Jan 1998) Function of a glutathione-dependent formaldehyde dehydrogenase in Rhodobacter sphaeroides formaldehyde oxidation and assimilation., Biochemistry, 37 (2), 530-7 Abstract
  • Flory JE, Donohue TJ (Oct 1997) Transcriptional control of several aerobically induced cytochrome structural genes in Rhodobacter sphaeroides., Microbiology (Reading, England), 143 ( Pt 10), 3101-10 Abstract
  • Donohue TJ (May 1997) Eubacterial signal transduction by ligands of the mammalian peripheral benzodiazepine receptor complex., Proceedings of the National Academy of Sciences of the United States of America, 94 (10), 4821-2 Abstract
  • Witthuhn VC Jr, Gao J, Hong S, Halls S, Rott MA, Wraight CA, Crofts AR, Donohue TJ, Reactions of isocytochrome c2 in the photosynthetic electron transfer chain of Rhodobacter sphaeroides., Biochemistry, 36(4), 903-11, 28 1997 Abstract
  • Flory, J. E., and T. J. Donohue, Transcriptional control of several aerobically- induced cytochrome structural genes in Rhodobacter sphaeroides, Microbiology, 143, 3101-3110, 1997
  • Bintrim SB, Donohue TJ, Handelsman J, Roberts GP, Goodman RM (Jan 1997) Molecular phylogeny of Archaea from soil., Proceedings of the National Academy of Sciences of the United States of America, 94 (1), 277-82 Abstract
  • Witthuhn VC, Gao J, Hong S, Halls S, Rott MA, Wraight CA, Crofts AR, Donohue TJ (Jan 1997) Reactions of isocytochrome c2 in the photosynthetic electron transfer chain of Rhodobacter sphaeroides., Biochemistry, 36 (4), 903-11 Abstract
  • Barber RD, Rott MA, Donohue TJ (Mar 1996) Characterization of a glutathione-dependent formaldehyde dehydrogenase from Rhodobacter sphaeroides., Journal of bacteriology, 178 (5), 1386-93 Abstract
  • Schilke BA, Donohue TJ, ChrR positively regulates transcription of the Rhodobacter sphaeroides cytochrome c2 gene., Journal of Bacteriology, 177(8), 1929-37, 1995 Abstract
  • Flory J E, Donohue T J, Organization and expression of the Rhodobacter sphaeroides cycFG operon, Journal of Bacteriology, 177, 4311-20, 1995 Abstract
  • Brandner JP, Donohue TJ, The Rhodobacter sphaeroides cytochrome c2 signal peptide is not necessary for export and heme attachment., Journal of Bacteriology, 176(3), 602-9, 1994 Abstract
  • Karls R K, Jin D J, Donohue T J, Transcription properties of RNA polymerase holoenzymes isolated from the purple nonsulfur bacterium Rhodobacter sphaeroides [published erratum appears in J Bacteriol 1994 Feb;176(3):938], Journal of Bacteriology, 175, 7629-38, 1993 Abstract
  • Mecsas J, Rouviere P E, Erickson J W, Donohue T J, Gross C A, The activity of sigma E, an Escherichia coli heat-inducible sigma-factor, is modulated by expression of outer membrane proteins, Genes and Development, 7, 2618-28, 1993 Abstract
  • Rott M A, Witthuhn V C, Schilke B A, Soranno M, Ali A, Donohue T J, Genetic evidence for the role of isocytochrome c2 in photosynthetic growth of Rhodobacter sphaeroides Spd mutants, Journal of Bacteriology, 175, 358-66, 1993 Abstract
  • Schilke B A,Donohue T J, delta-Aminolevulinate couples cycA transcription to changes in heme availability in Rhodobacter sphaeroides, Journal of Molecular Biology, 226, 101-15, 1992 Abstract
  • Rott M A, Fitch J, Meyer T E, Donohue T J, Regulation of a cytochrome c2 isoform in wild-type and cytochrome c2 mutant strains of Rhodobacter sphaeroides, Archives of Biochemistry and Biophysics, 292, 576-82, 1992 Abstract
  • MacGregor B J, Donohue T J, Evidence for two promoters for the cytochrome c2 gene (cycA) of Rhodobacter sphaeroides, Journal of Bacteriology, 173, 3949-57, 1991 Abstract
  • Brandner J P, Stabb E V, Temme R, Donohue T J, Regions of Rhodobacter sphaeroides cytochrome c2 required for export, heme attachment, and function, Journal of Bacteriology, 173, 3958-65, 1991 Abstract
  • Wu Y Q, MacGregor B J, Donohue T J, Kaplan S, Yen B, Genetic and physical mapping of the Rhodobacter sphaeroides photosynthetic gene cluster from R-prime pWS2, Plasmid, 25, 163-76, 1991 Abstract
  • Rott M A, Donohue T J, Rhodobacter sphaeroides spd mutations allow cytochrome c2-independent photosynthetic growth, Journal of Bacteriology, 172, 1954-61, 1990 Abstract
  • Fitch J, Cannac V, Meyer T E, Cusanovich M A, Tollin G, Van Beeumen J, Rott M A, Donohue T J, Expression of a cytochrome c2 isozyme restores photosynthetic growth of Rhodobacter sphaeroides mutants lacking the wild-type cytochrome c2 gene, Archives of Biochemistry and Biophysics, 271, 502-7, 1989 Abstract
  • Brandner J P, McEwan A G, Kaplan S, Donohue T J, Expression of the Rhodobacter sphaeroides cytochrome c2 structural gene, Journal of Bacteriology, 171, 360-8, 1989 Abstract
  • McEwan A G, Kaplan S, Donohue T J, Synthesis of Rhodobacter sphaeroides cytochrome c2 in Escherichia coli, Fems Microbiology Letters, 50, 253-8, 1989 Abstract
  • Sockett R E, Donohue T J, Varga A R, Kaplan S, Control of photosynthetic membrane assembly in Rhodobacter sphaeroides mediated by puhA and flanking sequences, Journal of Bacteriology, 171, 436-46, 1989 Abstract
  • DeHoff BS, Lee JK, Donohue TJ, Gumport RI, Kaplan S (Oct 1988) In vivo analysis of puf operon expression in Rhodobacter sphaeroides after deletion of a putative intercistronic transcription terminator., Journal of bacteriology, 170 (10), 4681-92 Abstract
  • Donohue TJ, McEwan AG, Van Doren S, Crofts AR, Kaplan S (Mar 1988) Phenotypic and genetic characterization of cytochrome c2 deficient mutants of Rhodobacter sphaeroides., Biochemistry, 27 (6), 1918-25 Abstract
  • Davis J, Donohue TJ, Kaplan S (Jan 1988) Construction, characterization, and complementation of a Puf- mutant of Rhodobacter sphaeroides., Journal of bacteriology, 170 (1), 320-9 Abstract
  • Lynn SP, Burton WS, Donohue TJ, Gould RM, Gumport RI, Gardner JF (Mar 1987) Specificity of the attenuation response of the threonine operon of Escherichia coli is determined by the threonine and isoleucine codons in the leader transcript., Journal of molecular biology, 194 (1), 59-69 Abstract
  • Kiley PJ, Donohue TJ, Havelka WA, Kaplan S (Feb 1987) DNA sequence and in vitro expression of the B875 light-harvesting polypeptides of Rhodobacter sphaeroides., Journal of bacteriology, 169 (2), 742-50 Abstract
  • Donohue TJ, Hoger JH, Kaplan S (Nov 1986) Cloning and expression of the Rhodobacter sphaeroides reaction center H gene., Journal of bacteriology, 168 (2), 953-61 Abstract
  • Donohue TJ, McEwan AG, Kaplan S (Nov 1986) Cloning, DNA sequence, and expression of the Rhodobacter sphaeroides cytochrome c2 gene., Journal of bacteriology, 168 (2), 962-72 Abstract
  • Zhu YS, Kiley PJ, Donohue TJ, Kaplan S (Aug 1986) Origin of the mRNA stoichiometry of the puf operon in Rhodobacter sphaeroides., The Journal of biological chemistry, 261 (22), 10366-74 Abstract
  • Donohue TJ, Chory J, Goldsand TE, Lynn SP, Kaplan S (Jul 1985) Structure and physical map of Rhodopseudomonas sphaeroides bacteriophage RS1 DNA., Journal of virology, 55 (1), 147-57 Abstract
  • Chory J, Donohue TJ, Varga AR, Staehelin LA, Kaplan S (Aug 1984) Induction of the photosynthetic membranes of Rhodopseudomonas sphaeroides: biochemical and morphological studies., Journal of bacteriology, 159 (2), 540-54 Abstract
  • Bernlohr RW, Schreier HJ, Donohue TJ (1984) Enzymes of glutamate and glutamine biosynthesis in Bacillus licheniformis., Current topics in cellular regulation, 24, 145-52 Abstract
  • Cain BD, Donohue TJ, Shepherd WD, Kaplan S (Jan 1984) Localization of phospholipid biosynthetic enzyme activities in cell-free fractions derived from Rhodopseudomonas sphaeroides., The Journal of biological chemistry, 259 (2), 942-8 Abstract
  • Cain BD, Singer M, Donohue TJ, Kaplan S (Oct 1983) In vivo metabolic intermediates of phospholipid biosynthesis in Rhodopseudomonas sphaeroides., Journal of bacteriology, 156 (1), 375-85 Abstract
  • Kaplan S, Cain BD, Donohue TJ, Shepherd WD, Yen GS (1983) Biosynthesis of the photosynthetic membranes of Rhodopseudomonas sphaeroides., Journal of cellular biochemistry, 22 (1), 15-29 Abstract
  • Donohue TJ, Cain BD, Kaplan S (Nov 1982) Alterations in the phospholipid composition of Rhodopseudomonas sphaeroides and other bacteria induced by Tris., Journal of bacteriology, 152 (2), 595-606 Abstract
  • Cain BD, Donohue TJ, Kaplan S (Nov 1982) Kinetic analysis of N-acylphosphatidylserine accumulation and implications for membrane assembly in Rhodopseudomonas sphaeroides., Journal of bacteriology, 152 (2), 607-15 Abstract
  • Donohue TJ, Bernlohr RW (Oct 1981) Regulation of the activity of the Bacillus licheniformis A5 glutamine synthetase., Journal of bacteriology, 148 (1), 174-82 Abstract
  • Donohue TJ, Bernlohr RW (Aug 1981) Properties of the Bacillus licheniformis A5 glutamine synthetase purified from cells grown in the presence of ammonia or nitrate., Journal of bacteriology, 147 (2), 589-601 Abstract
  • Kalb VF, Donohue TJ, Corrigan MG, Bernlohr RW (Oct 1978) A new and specific assay for ammonia and glutamine sensitive to 100 pmol., Analytical biochemistry, 90 (1), 47-57 Abstract
  • Donohue TJ, Bernlohr RW (Aug 1978) Effect of cultural conditions on the concentrations of metabolic intermediates during growth and sporulation of Bacillus licheniformis., Journal of bacteriology, 135 (2), 363-72 Abstract
  • Siegel WH, Donohue T, Bernlohr RW (Nov 1977) Determination of pools of tricarboxylic acid cycle and related acids in bacteria., Applied and environmental microbiology, 34 (5), 512-7 Abstract
  • Melnick RL, Donohue T (Mar 1976) Use of an adenosine triphosphate analog, adenylyl imidodiphosphate, to evaluate adenosine triphosphate-dependent reactions in mitochondria., Archives of biochemistry and biophysics, 173 (1), 231-6 Abstract
  • Meyer, T. E. and T. J. Donohue. 1995. Cytochromes, iron-sulfur and copper proteins mediating electron transfer from the cyt bc1 complex to photosynthetic reaction center complexes. pp. 725-745. In. Blankenship, R. E., M. T. Madigan and C. E. Bauer, eds. Anoxygenic Photosynthetic Bacteria. Advances in Photosynthesis, Kluwer Academic Publishers, Dordrecht, Netherlands

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Last Verified: 3/17/2010

COS Expertise ID #309524
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