Dr. Ellen L. Neidle

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University of Georgia
Arts and Sciences
ProfessorAppointed: 2004
Professional Headshot of Ellen L. Neidle

Mailing Address

Microbiology Department
527 Biological Sciences Building
Athens, Georgia 30602-2605
United States

Contact Information

Phone: (706) 542-2852
Fax: (706) 542-2674
eneidle@uga.edu

Qualifications

Ph.D., Yale University, Biology, 1987.
M.Phil., Yale University, Biology, 1985.
M.S., Yale University, Biology, 1984.
B.S., Yale University, Molecular Biophysics and Biochemistry, 1981.

Expertise and Research Interests

Interests include:
Bacterial aromatic compound degradation
Transcriptional regulation
LysR-type transcriptional regulatory proteins
Acinetobacter physiology
Substrate specificity of enzymes such as dioxygenases
Protein structure and function
Gene amplification and genome plasticity
Chromosomal organization and supraoperonic gene clustering

Keywords

COS Keywords:

Bioremediation, Genomics, Microbiology, Physiology, Protein Structure, Transcription.

Additional Terms:

Aromatic Compound Degradation, Bioremediation, Regulation.

Memberships

American Society for Microbiology

Honors and Awards

2005, Creative Research Medal, University of Georgia Research Foundation
1988-1991, Inaugural Lawton Chiles Fellowship in Biotechnology, NIH
1985-1988, Yale-Celgene Biotechnology Program Fellowship, Celgene
1982-1985, National Research Service Award Training Grant, NIH, Yale University

Previous Positions

1999-2004, Associate Professor, University of Georgia
1993-1993, Senior Scientist, Isogenetics, Inc., Chicago Technology Park
1993-1999, Assistant Professor, University of Georgia
1988-1992, Postdoctoral Research Associate, University of Illinois, Laboratory of Dr. Samuel Kaplan
1988-1988, Visiting Researcher, University of Geneva
1988-1992, Postdoctoral Research Associate, University of Texas Health Science Center at Houston
1987-1988, Postdoctoral Associate, Yale-Celgene Biotechnology Program

Publications

  • Craven SH, Neidle EL (Jun 2007) Double trouble: medical implications of genetic duplication and amplification in bacteria., Future microbiology, 2, 309-21 Abstract
  • Ezezika OC, Haddad S, Neidle EL, Momany C (May 2007) Oligomerization of BenM, a LysR-type transcriptional regulator: structural basis for the aggregation of proteins in this family., Acta crystallographica. Section F, Structural biology and crystallization communications, 63 (Pt 5), 361-8 Abstract
  • Ezezika OC, Haddad S, Clark TJ, Neidle EL, Momany C (Mar 2007) Distinct effector-binding sites enable synergistic transcriptional activation by BenM, a LysR-type regulator., Journal of molecular biology, 367 (3), 616-29 Abstract
  • Ezezika OC, Collier-Hyams LS, Dale HA, Burk AC, Neidle EL (Mar 2006) CatM regulation of the benABCDE operon: functional divergence of two LysR-type paralogs in Acinetobacter baylyi ADP1., Applied and environmental microbiology, 72 (3), 1749-58 Abstract
  • Reams AB, Neidle EL, Gene amplification involves site-specific short homology-independent illegitimate recombination in Acinetobacter sp. strain ADP1, Journal of Molecular Biology, 338(4), 643-56, 2004 Abstract
  • Buchan A, Neidle EL, Moran MA, Diverse organization of genes of the beta-ketoadipate pathway in members of the marine Roseobacter lineage, Applied and Environmental Microbiology, 70(3), 1658-68, 2004 Abstract
  • Clark TJ, Phillips RS, Bundy BM, Momany C, Neidle EL, Benzoate decreases the binding of cis,cis-muconate to the BenM regulator despite the synergistic effect of both compounds on transcriptional activation, Journal of Bacteriology, 186(4), 1200-4, 2004 Abstract
  • Clark T, Haddad S, Neidle E, Momany C, Crystallization of the effector-binding domains of BenM and CatM, LysR-type transcriptional regulators from Acinetobacter sp. ADP1, Acta Crystallographica. Section D, Biological Crystallography, 60(Pt 1), 105-8, January 2004 Abstract
  • Reams AB, Neidle EL, Selection for Gene Clustering By Tandem Duplication., Annual Review of Microbiology, 58, 119-42, 2004 Abstract
  • Beharry ZM, Eby DM, Coulter ED, Viswanathan R, Neidle EL, Phillips RS, Kurtz DM Jr, Histidine ligand protonation and redox potential in the rieske dioxygenases: role of a conserved aspartate in anthranilate 1,2-dioxygenase, Biochemistry, 42(46), 13625-36, November 2003 Abstract
  • Reams AB, Neidle EL, Genome plasticity in Acinetobacter: new degradative capabilities acquired by the spontaneous amplification of large chromosomal segments, Molecular Microbiology, 47(5), 1291-304, 2003 Abstract
  • Brzostowicz PC, Reams AB, Clark TJ, Neidle EL, Transcriptional cross-regulation of the catechol and protocatechuate branches of the beta-ketoadipate pathway contributes to carbon source-dependent expression of the Acinetobacter sp. str, Applied and Environmental Microbiology, 69(3), 1598-606, 2003 Abstract
  • Cosper NJ, Eby DM, Kounosu A, Kurosawa N, Neidle EL, Kurtz DM Jr, Iwasaki T, Scott RA, Redox-dependent structural changes in archaeal and bacterial Rieske-type [2Fe-2S] clusters, Protein Science: A Publication of the Protein Society, 11(12), 2969-73, December 2002
  • Bundy BM, Collier LS, Hoover TR, Neidle EL, Synergistic transcriptional activation by one regulatory protein in response to two metabolites, Proceedings of the National Academy of Sciences (USA), 99(11), 7693-8, May 2002 Abstract
  • Karlsson A, Beharry ZM, Matthew Eby D, Coulter ED, Neidle EL, Kurtz DM Jr, Eklund H, Ramaswamy S, X-ray crystal structure of benzoate 1,2-dioxygenase reductase from Acinetobacter sp. strain ADP1, Journal of Molecular Biology, 318(2), 261-72, April 2002 Abstract
  • Clark TJ, Momany C, Neidle EL, The benPK operon, proposed to play a role in transport, is part of a regulon for benzoate catabolism in Acinetobacter sp. strain ADP1, Microbiology (Reading, England), 148(Pt 4), 1213-23, April 2002
  • Buchan A, Neidle EL, Moran MA, Diversity of the ring-cleaving dioxygenase gene pcaH in a salt marsh bacterial community, Applied and Environmental Microbiology, 67(12), 5801-9, December 2001 Abstract
  • Haddad S, Eby DM, Neidle EL, Cloning and expression of the benzoate dioxygenase genes from Rhodococcus sp. strain 19070, Applied and Environmental Microbiology, 67(6), 2507-14, June 2001 Abstract
  • Eby DM, Beharry ZM, Coulter ED, Kurtz DM Jr, Neidle EL, Characterization and evolution of anthranilate 1,2-dioxygenase from Acinetobacter sp. strain ADP1, Journal of Bacteriology, 183(1), 109-18, Jan 2001 Abstract
  • Cosper NJ, Collier LS, Clark TJ, Scott RA, Neidle EL, Mutations in catB, the gene encoding muconate cycloisomerase, activate transcription of the distal ben genes and contribute to a complex regulatory circuit in Acinetobacter sp. strain ADP1, Journal of Bacteriology, 182(24), 7044-52, December 2000 Abstract
  • Buchan A, Collier LS, Neidle EL, Moran MA, Key aromatic-ring-cleaving enzyme, protocatechuate 3,4-dioxygenase, in the ecologically important marine Roseobacter lineage, Applied and Environmental Microbiology, 66(11), 4662-72, November 2000 Abstract
  • Jones RM, Collier LS, Neidle EL, Williams PA, areABC genes determine the catabolism of aryl esters in Acinetobacter sp. Strain ADP1, Journal of Bacteriology, 181(15), 4568-75, August 1999 Abstract
  • Bundy BM, Campbell AL, Neidle EL, Similarities between the antABC-encoded anthranilate dioxygenase and the benABC-encoded benzoate dioxygenase of Acinetobacter sp. strain ADP1, Journal of Bacteriology, 180(17), 4466-74, September 1998 Abstract
  • Collier LS, Gaines GL, Neidle EL, Regulation of benzoate degradation in Acinetobacter sp. strain ADP1 by BenM, a LysR-type transcriptional activator, Journal of Bacteriology, 180(9), 2493-501, May 1998 Abstract
  • Collier LS, Nichols NN, Neidle EL, benK encodes a hydrophobic permease-like protein involved in benzoate degradation by Acinetobacter sp. strain ADP1, Journal of Bacteriology, 179(18), 5943-6, September 1997 Abstract
  • Gralton EM, Campbell AL, Neidle EL, Directed introduction of DNA cleavage sites to produce a high-resolution genetic and physical map of the Acinetobacter sp. strain ADP1 (BD413UE) chromosome, Microbiology, 143, (Pt 4) 1345-57, April 1997
  • Gaines GL, Smith L, Neidle EL, Novel nuclear magnetic resonance spectroscopy methods demonstrate preferential carbon source utilization by Acinetobacter calcoaceticus, Journal of Bacteriology, 178(23), 6833-41, December 1996 Abstract
  • Romero-Arroyo CE, Schell MA, Gaines GL, Neidle EL, catM encodes a LysR-type transcriptional activator regulating catechol degradation in Acinetobacter calcoaceticus, Journal of Bacteriology, 177(20), 5891-8, October 1995 Abstract
  • Neidle EL, Kaplan S, Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes, Journal of Bacteriology, 175(8), 2292-303, April 1993 Abstract
  • Neidle EL, Kaplan S, 5-Aminolevulinic acid availability and control of spectral complex formation in hemA and hemT mutants of Rhodobacter sphaeroides, Journal of Bacteriology, 175(8), 2304-13, April 1993 Abstract
  • Neidle EL, Kaplan S, Rhodobacter sphaeroides rdxA, a homolog of Rhizobium meliloti fixG, encodes a membrane protein which may bind cytoplasmic [4Fe-4S] clusters, Journal of Bacteriology, 174(20), 6444-54, October 1992 Abstract
  • Neidle E, Hartnett C, Ornston LN, Bairoch A, Rekik M, Harayama S, cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily, European Journal of Biochemistry, 204(1), 113-20, 1992 Abstract
  • Harayama S, Kok M, Neidle EL, Functional and evolutionary relationships among diverse oxygenases, Annual Review of Microbiology, 46, 565-601, 1992 Abstract
  • Harayama S, Rekik M, Bairoch A, Neidle EL, Ornston LN, Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWW0 plasmid xylXYZ, genes encoding benzoate diox, Journal of Bacteriology, 173(23), 7540-8, December 1991 Abstract
  • Neidle EL, Hartnett C, Ornston LN, Bairoch A, Rekik M, Harayama S, Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary relationships among multicomponent oxygenases, Journal of Bacteriology, 173(17), 5385-95, September 1991 Abstract
  • Hartnett C, Neidle EL, Ngai KL, Ornston LN, DNA sequences of genes encoding Acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: Evidence indicating shuffling of genes and of DNA sequences within genes during their evolutionary divergen, Journal of Bacteriology, 172(2), 956-66, February 1990
  • Ngai KL, Neidle EL, Ornston LN, Catechol and chlorocatechol 1,2-dioxygenases, Methods in Enzymology, 188, 122-6, 1990 Abstract
  • Neidle EL, Hartnett C, Ornston LN, Characterization of Acinetobacter calcoaceticus catM, a repressor gene homologous in sequence to transcriptional activator genes, Journal of Bacteriology, 171(10), 5410-21, October 1989 Abstract
  • Neidle EL, Hartnett C, Bonitz S, Ornston LN, DNA sequence of the Acinetobacter calcoaceticus catechol 1,2-dioxygenase I structural gene catA: Evidence for evolutionary divergence of intradiol dioxygenases by acquisition of DNA sequence repetitio, Journal of Bacteriology, 170(10), 4874-80, October 1988
  • Neidle EL, Shapiro MK, Ornston LN, Cloning and expression in Escherichia coli of Acinetobacter calcoaceticus genes for benzoate degradation, Journal of Bacteriology, 169(12), 5496-503, December 1987 Abstract
  • Neidle EL, Ornston LN, Benzoate and muconate, structurally dissimilar metabolites, induce expression of catA in Acinetobacter calcoaceticus, Journal of Bacteriology, 169(1), 414-5, January 1987 Abstract
  • Neidle EL, Ornston LN, Cloning and expression of Acinetobacter calcoaceticus catechol 1,2-dioxygenase structural gene catA in Escherichia coli, Journal of Bacteriology, 168(2), 815-20, November 1986 Abstract
  • Shanley MS, Neidle EL, Parales RE, Ornston LN, Cloning and expression of Acinetobacter calcoaceticus catBCDE genes in Pseudomonas putida and Escherichia coli, Journal of Bacteriology, 165(2), 557-63, February 1986 Abstract
  • Kelly JA, Neidle EL, Neidle A, An aminopeptidase from mouse brain cytosol that cleaves N-terminal acidic amino acid residues, Journal of Neurochemistry, 40(6), 1727-34, June 1983 Abstract

Profile Details

Last Updated: 2/3/2008

COS Expertise ID #1025834
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