Dr. Frederick D. Quinn

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University of Georgia
College of Veterinary Medicine
Medical Microbiology and Parasitology
Professor and HeadAppointed: 2002
University of Georgia
Biomedical and Health Sciences Institute
Infectious Diseases
ChairAppointed: 2002

Mailing Address

College of Veterinary Medicine
Athens, Georgia 30602
United States

Contact Information

Phone: (706) 542-5790
Fax: (706) 542-5771
fquinn@vet.uga.edu
http://www.biomed.uga.edu/mem_quinn_fred.htm

Qualifications

Ph.D., Indiana University Bloomington, Microbiology, 1986.
M.A., Indiana University Bloomington, Microbiology, 1982.
B.S., Marquette University, Biology, 1980.

Expertise and Research Interests

Identify host and bacterial factors associated with latent Mycobacterium tuberculosis infections: The goal of our research effort is to identify bacterial and host factors that contribute to the development and maintenance of latent M. tuberculosis infections. We have developed or improved upon two in vitro models (alveolar bilayer and granuloma) of latent infection, and have identified several M. tuberculosis genes (sigma factors and other regulators) and gene products that are expressed exclusively during survival in these systems and corresponding animal models. One gene product in particular (alpha-crystallin homolog) is being examined as a marker of latent infection.

Other Expertise

Identification of host bacterial factors responsible for the HIV/MTB induction synergy: The goal of this research effort is to identify the bacterial and host cell factors that contribute to enhanced HIV production during M. tuberculosis coinfection. We have developed an in vitro human PBMC model that can mimic some events observed in vivo. With this model we observed numerous cytokines, surface markers and signaling cascades from human PBMCs upregulated during HIV - M. tuberculosis coinfection that are not detected during single infection or coinfection with HIV and M. bovis BCG.

Future Research

Mycobacterial infections in zebrafish: The natural fish and frog pathogen, Mycobacterium marinum, is a good model for human tuberculosis infection since the genomes of M. marinum and M. tuberculosis are closely related and infections produce similar pathology in their respective hosts. The objectives of this research effort are to examine the pathogenicity of M. marinum infections in zebrafish with the aim of developing this system into a model of host-parasite interaction. Specifically, we will generate reagents to analyze the immune response including cell lines and monoclonal antibodies against specific surface markers. In addition, we will screen zebrafish mutations required for the establishment of M. marinum infections.

Industrial Relevance

Development of a medical device for the diagnosis of latent tuberculosis: The major goal of this project is to develop an in vitro diagnostic test for the diagnosis of latent tuberculosis in serum or urine specimens. The M. tuberculosis alpha-crystallinprotein is expressed exclusively during latent infection or during logarithmic phase culture. We are currently examining the efficacy of a test that will quantitate the alpha-crystallin protein in the serum or urine of skin test positive individuals that do not show signs of active infection.

Keywords

COS Keywords:

Infectious Diseases or Agents, Microbiology, Molecular Biology.

Additional Terms:

Infectious Diseases, Microbiology, Molecular Biology.

Memberships

American Society for Microbiology
Society for In-Vitro Biology

Honors and Awards

2001, Honor Award, Centers for Disease Control and Prevention
1999, Fulbright Scholar, Fulbright Commission
1996-1998, Trudeau Young Investigator Award, American Lung Association
1995, James K. Nakano Citation, Center for Disease Control and Prevention
1994, Secretary's Award, Department of Health and Human Services
1992, Director's Award, Centers for Disease Control

Previous Positions

1996-2002, Chief, National Center for Infectious Diseases, Division of AIDS, STD and Tuberculosis Laboratory Research, Tuberculosis/Mycobacteriology Branch, Pathogenesis Laboratory

Patents

M. tuberculosis alpha-crystallin protein as a potential diagnostic of latent infection, Patent Number: pending, 2003, Institution-owned, United States.
Invasion associated gene product from Neisseria meningitidis serogroup B as a potential vaccine candidate, Patent Number: pending, 2003, Institution-owned, United States.
secA gene of Mycobacterium tuberculosis and related methods and compositions, Patent Number: 5885828, 1999, Institution-owned, United States of America.
Artificial organ culture system for the examination of human lung pathogens, Patent Number: 5750329, 1998, Institution-owned, United States of America.
An artificial organ culture system for examining host-parasite and host-drug interactions, Patent Number: 5695996, 1997, Institution-owned, United States of America.

Funding Received

  • Canada Foundation for Innovation (CFI): Development of a medical device for the diagnosis of latent tuberculosis, Nov 2002 to Oct 2004.
  • National Institutes of Health (NIH): Regulation of the host response genes involved in the pathogenesis of pulmonary tuberculosis, Jul 1, 2002 to Jun 30, 2007.
  • Veterinary Experimental Experiment Station: Identifying virulence mechanisms of Mycobacterium shottsii: an emerging pathogen of fish, Jul 1, 2002 to May 31, 2003.

Publications

  • Castro-Garza J, King CH, Swords WE, Quinn FD, Demonstration of spread by Mycobacterium tuberculosis bacilli in A549 epithelial cell monolayers, Fems Microbiology Letters, 212(2), 145-9, July 2002 Abstract
  • Owens MU, Swords WE, Schmidt MG, King CH, Quinn FD, Cloning, expression, and functional characterization of the Mycobacterium tuberculosis secA gene, Fems Microbiology Letters, 211(2), 133-41, June 2002 Abstract
  • Dobos KM, Small PL, Deslauriers M, Quinn FD, King CH, Mycobacterium ulcerans cytotoxicity in an adipose cell model, Infection and Immunity, 69(11), 7182-6, November 2001 Abstract
  • Rhodes MW, Kator H, Kotob S, van Berkum P, Kaattari I, Vogelbein W, Floyd MM, Butler WR, Quinn FD, Ottinger C, Shotts E, A unique Mycobacterium species isolated from an epizootic of striped bass (Morone saxatilis), Emerging Infectious Diseases, 7(5), 896-9, 2001 Abstract
  • Hudson CR, Frye JG, Quinn FD, Gherardini FC, Increased expression of Borrelia burgdorferi vlsE in response to human endothelial cell membranes, Molecular Microbiology, 41(1), 229-39, July 2001 Abstract
  • Jensen-Cain DM, Quinn FD, Differential expression of sigE by Mycobacterium tuberculosis during intracellular growth, Microbial Pathogenesis, 30(5), 271-8, May 2001 Abstract
  • Dobos KM, Spotts EA, Quinn FD, King CH, Necrosis of lung epithelial cells during infection with Mycobacterium tuberculosis is preceded by cell permeation, Infection and Immunity, 68(11), 6300-10, November 2000 Abstract
  • Long EG, Ewing EP Jr, Bartlett JH, Horsburgh CR Jr, Birkness KA, Yakrus MA, Newman GW, Quinn FD, Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs, Fems Microbiology Letters, 190(2), 267-72, September 2000 Abstract
  • Dezzutti, C.S., W.E. Swords, P.C. Guenthner, D.R. Sasso, L.M. Wahl, A.H. Drummond, G.W. Newman, C.H. King, F.D. Quinn, and R.B. Lal., Involvement of matrix metalloproteinases in human immunodeficiency virus type 1-induced replication by clinical Mycobacterium avium isolates, Journal of Infectious Diseases, 180, 1142-1152, 1999
  • Birkness, K.A., W.E. Swords, P-H. Huang, E.H. White, C.S. Dezzutti, R.B. Lal, and F.D. Quinn., Observed differences in virulence-associated phenotypes between a human clinical isolate and a veterinary isolate of Mycobacterium avium, Infection and Immunity, 67, 4895-901, 1999
  • Birkness, K.A., M. Deslauriers, J.H. Bartlett, E.H. White, C.H. King, and F.D. Quinn., An in vitro tissue culture bilayer model to examine early events in Mycobacterium tuberculosis infection, Infection and Immunity, 67, 653-658, 1999
  • Dobos, K.A., F. D. Quinn, D.A. Ashford, C.R. Horsburgh, and C.H. King., Emergence of a unique group of necrotizing mycobacteria, Emerging Infectious Diseases, 5, 367-378, 1999
  • Steinert, M., K. Birkness, E. White, B. Fields, and F. Quinn., Mycobacterium avium bacilli grow saprozoically in coculture with Acanthamoeba polyphaga and survive within cyst walls, Applied and Environmental Microbiology, 64, 2256-2261, 1998
  • Ribot, E.M., F.D. Quinn, X. Bai, J.J. Murtagh, Jr., Rapid amplification of transposon ends for the isolation, cloning and sequencing of transposon disrupted chromosomal genes, Biotechniques, 24, 16-22, 1998
  • Quinn, F.D., G.W. Newman, and C.H. King., In search of virulence factors of human bacterial disease, Trends in Microbiology, 5, 20-26, 1997
  • Quinn, F.D., G.W. Newman, C.H. King., Virulence determinants of Mycobacterium tuberculosis, In: Tuberculosis. T. Shinnick, ed. Curr. Topics in Microbiol. Immunol., pp. 131-156, 1996
  • Mehta, P.K., C.H. King, E.H. White, J.J. Murtagh, Jr., F.D. Quinn., Comparison of in vitro models for the study of Mycobacterium tuberculosis invasion and intracellular replication, Infection and Immunity, 164, 2673-2679, 1996
  • Fischer, L.J., F.D. Quinn, E.H. White, and C.H. King., Intracellular growth and cytotoxicity of Mycobacterium haemophilum in a human epithelial cell line, HecIB, Infection and Immunity, 64, 269-276, 1996
  • Pine, L., F.D. Quinn, E.P. Ewing, Jr., K.A. Birkness, E.H. White, D.S. Stephens, E. Ribot., Evaluation of the chick embryo for the determination of relative virulence of Neisseria meningitidis, FEMS Microbiology Letters, 130, 37-44, 1995
  • Quinn, F.D., R.S. Weyant, M. J. Worley, E.H. White, E.A. Utt, and E.A. Ades., Human microvascular endothelial tissue culture cell model for studying the pathogenesis of Brazilian purpuric fever, Infection and Immunity, 63, 2317-2322, 1995
  • Utt, E.A., J.P. Brousal, L.C. Kikuta-Oshima and F.D. Quinn., The identification of bacterial gene expression differences using mRNA-based isothermal subtractive hybridization, Canadian Journal of Microbiology, 41, 152-156, 1995
  • Tondella, M.L.C., F.D. Quinn, and B.A. Perkins., Brazilian purpuric fever caused by Haemophilus influenzae biogroup aegyptius strains lacking the 3031 plasmid, Journal of Infectious Diseases, 171, 209-211, 1995
  • Kikuta-Oshima LC, King CH, Shinnick TM, Quinn FD, Methods for the identification of virulence genes expressed in Mycobacterium tuberculosis strain H37Rv, Annals of the New York Academy of Sciences, 730, 263-5, August 1994 Abstract
  • Weyant RS, Quinn FD, Utt EA, Worley M, George VG, Candal FJ, Ades EW, Human microvascular endothelial cell toxicity caused by Brazilian purpuric fever-associated strains of Haemophilus influenzae biogroup aegyptius, Journal of Infectious Diseases, 169(2), 430-3, February 1994 Abstract
  • Weyant, R.A., F.D. Quinn, M.J. Worley, V.G. George, E.H. White and E.A. Utt., Tissue culture assay that differentiates virulent from avirulent strains of Haemophilus influenzae, biogroup aegyptius, Journal of Infectious Diseases, 169, 430-433, 1994
  • Quinn FD, Weyant RS, Candal FJ, Ades EW, Destruction of human microvascular endothelial cell capillary-like microtubules by Brazilian purpuric fever-associated Haemophilus influenzae biogroup aegyptius, Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology., 62(2), 109-12, 1994 Abstract
  • Koehler JE, Quinn FD, Berger TG, LeBoit PE, Tappero JW, Isolation of Rochalimaea species from cutaneous and osseous lesions of bacillary angiomatosis, The New England Journal of Medicine, 327(23), 1625-31, December 1992 Abstract
  • Birkness KA, George VG, White EH, Stephens DS, Quinn FD, Intracellular growth of Afipia felis, a putative etiologic agent of cat scratch disease, Infection and Immunity, 60(6), 2281-7, June 1992 Abstract
  • Farley MM, Whitney AM, Spellman P, Quinn FD, Weyant RS, Mayer L, Stephens DS, Analysis of the attachment and invasion of human epithelial cells by Haemophilus influenzae biogroup aegyptius, Journal of Infectious Diseases, 165 Suppl 1, S111-4, June 1992 Abstract
  • McIntyre M, Quinn FD, Fields PI, Berdal BP, Rapid identification of Legionella pneumophila zinc metalloprotease using chromogenic detection, Apmis : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 99(4), 316-20, April 1991 Abstract
  • Black WJ, Quinn FD, Tompkins LS, Legionella pneumophila zinc metalloprotease is structurally and functionally homologous to Pseudomonas aeruginosa elastase, Journal of Bacteriology, 172(5), 2608-13, May 1990 Abstract
  • Quinn FD, Keen MG, Tompkins LS, Genetic, immunological, and cytotoxic comparisons of Legionella proteolytic activities, Infection and Immunity, 57(9), 2719-25, September 1989 Abstract
  • Quinn FD, Tompkins LS, Analysis of a cloned sequence of Legionella pneumophila encoding a 38 kD metalloprotease possessing haemolytic and cytotoxic activities, Molecular Microbiology, 3(6), 797-805, June 1989 Abstract
  • Hoffman PS, Butler CA, Quinn FD, Cloning and temperature-dependent expression in Escherichia coli of a Legionella pneumophila gene coding for a genus-common 60-kilodalton antigen, Infection and Immunity, 57(6), 1731-9, June 1989 Abstract

Profile Details

Last Updated: 1/13/2003

COS Expertise ID #830640
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