Dr. Syed A. Hashsham

(Reading, Writing, Speaking)

Michigan Economic Development Corporation- Life Science Corridor (MEDC): Genescreen: A Low Cost and High Density DNA Biochip For Detecting Up To 30,000 Microorganisms, $1,190,812, Sep 15, 2003 to Sep 16, 2006.
Environmental Protection Agency (EPA): Bioinformatics Pilot Program for the Assessment of Virulence-Factor and Activity Relationships (VFARs) Waterborne Disease Microorganisms, $95,786, Sep 1, 2002 to Dec 31, 2003.
National Center for Manure Management (NCMM): Phosphorus Removal by Struvite Recovery, $35,000, Mar 1, 2003 to Dec 31, 2004.
National Science Foundation (NSF): Evaluation of Landfill Gas Emissions From an Instrumented Bioreactor Landfill Cell, $24,950, Jul 1, 2003 to Jul 30, 2004.
Environmental Protection Agency (EPA): Emerging Microbial Indicator Technologies, $20,497, Jan 1, 2003 to 2004.
Michigan Department of Environmental Quality (MI DEQ): Evaluation of Remediation in Schoolcraft Plumes G and F Contaminated with Chlorinated Solvents and Metals, $234,810, Jan 1, 2000 to Dec 31, 2003.
Department of Energy (DOE) and Environmental Research and Education Foundation (EREF): Landfill Bioreactors: Field Scale Evaluation, $207,000, Aug 16, 2001 to May 30, 2008.
Environmental Protection Agency (EPA): Characterization and Applications of Reductive Dehalogenase Genes, $249,842, Aug 1, 2002 to Jul 31, 2004.
National Science Foundation (NSF): Development of Microarrays for Evaluating Phylogenetic and Functional Diversity of the Microbial World, $25,000, Aug 1, 2002 to Jul 31, 2005.
National Institutes of Health-NIEHS: Health Hazards from Groundwater Contamination, $954,053, Apr 1, 2000 to Mar 31, 2004.
Environmental Protection Agency (EPA): On-Chip PCR, Nanoparticles, and Virulence/Marker Genes for Simultaneous Detection of 20 Waterborne Pathogens and Potential Indicator Organisms, $600,000, 2006 to 2009.
National Institutes of Health (NIH): Environmental, Microbial, and Mammalian Biomolecular Responses to Ahr Ligands (Co-PI): Project Leader. Dr. James Tiedje, Center Director: Dr. Norb Kaminski, 2006 to 2011.
EPA-DHS: Center for Microbial Risk Assessment (Co-PI) : Project Leader: Dr. Chuck Gerba; Center Directors- Dr. Joan Rose and Charles Haas, 2005 to 2010.
Environmental Protection Agency: DEVELOPMENT OF A VIRULENCE FACTOR BIOCHIP AND ITS VALIDATION FOR MICROBIAL RISK ASSESSMENT IN DRINKING WATER, $600,000, 2004 to 2007.
Department of Defense: REAL TIME SCANNING AND HYBRIDIZATION CAPABILITIES AT MSU FOR QUANTITATIVE AND PARALLEL DETECTION OF MICRORGANISMS ON DNA, $150,495, 2004 to 2005.
Waste Management Inc.: DEVELOPMENT OF AERATION STRATEGY FOR ELEVATING TEMPERATURE IN BIOREACTOR LANDFILL, $96,545, 2004 to 2005.
National Institutes of Health (NIH): Flexible Biochip for Highly Parallel Microbial Detection, $1,370,788, 2003 to 2006.
NATL INST OF ENVIRON HEALTH SCI.: Probing Microbial Communities By Stable Isotope And RNA/DNA Analyses, $223,804, 2003 to 2004.
National Science Foundation: EVALUATION OF LANDFILL GAS EMISSIONS FROM AN INSTRUMENTED BIOREACTOR LANDFILL CELL, $24,950, 2003 to 2004.
National Science Foundation: EVALUATION OF LANDFILL GAS EMISSIONS FROM AN INSTRUMENTED BIOREACTOR LANDFILL CELL, $44,652, 2003 to 2004.
Daimler Chrysler Corporation: GIFT TO BE USED FOR RESEARCH AT THE BIOREACTOR CELL AT NORTHERN OAKS LANDFILL, $50,000, 2003 to 2004.
MSU Case Center's Management of Technology and Innovation program: Assessing Market Potential for GeneScreen: A Low Cost and High Density DNA Biochip for Parallel Detection of up to 30,000 Microorganisms, $7,000, 2002 to 2002.
Intramural Research Grant Proposal (IRGP-MSU): Development of Experimental and Computational Tools in Environmental Genomics, $49,923, 2002 to 2003.
Michigan Animal Industry Coalition: Phosphorous Removal from Dairy Manure, $30,000, 2001 to 2002.
USEPA Central Region Hazardous Substance: State of Science: Landfill Bioreactors, $25,000, 2001 to 2002.
USEPA Western Region Hazardous Substance Research Center: Proof of Gene Expression during Bioremediation, $84,388, 1999 to 2001.

• Duran, M., Alm, E. W., Stedtfeld, R. D., Haznedaroglu, B.Z., and Hashsham, S.A. (2006) Detection and Occurrence of Indicator Organisms and Pathogens, Water Environment Research, Vol. 77, No. 6, pp.
• Lukas M. Wick, Jean Marie Rouillard, Thomas S. Whittam, Erdogan Gulari, James M. Tiedje and Syed A. Hashsham (2006) On-chip non-equilibrium dissociation curves and dissociation rate constants as methods to assess specificity of oligonucleotide probes, Nucleic Acids Research, 34(3):e26; doi:10.10
• Denef, V.J., Park, J., Tsoi, T.V., Rouillard, J.-M. , Zhang, H., Wibbenmeyer, J.A. , Verstraete, W., Gulari, E., Hashsham, S.A. , and J.M. Tiedje, (Polychlorinated) biphenyl metabolism in a genomic context: Outlining genome-wide metabolic networks in Burkholderia sp. LB400 relevant to PCB-biodegradation, Applied and Environmental Microbiology (In Press)., 2004
• Hashsham, S.A., Wick, L.M., Rouillard, J-M., Gulari, E., and J.M. Tiedje (2004) Potential of DNA microarrays for developing parallel detection tools (PDTs) for microorganisms relevant to biodefense and related research needs, Biosensors and Bioelectronics, Volume 20, Issue 4,
• Denef VJ, Park J, Rodrigues JL, Tsoi TV, Hashsham SA, Tiedje JM, Validation of a more sensitive method for using spotted oligonucleotide DNA microarrays for functional genomics studies on bacterial communities, Environmental Microbiology, 5(10), 933-43, October 2003
• Hashsham SA, Freedman DL, Adsorption of vitamin B12 to alumina, kaolinite, sand and sandy soil, Water Research, 37(13), 3189-93, July 2003
• Dollhopf SL, Pariseau ML, Hashsham SA, Tiedje JM, Competitive and interactions affecting a fermentative spirochete in anaerobic chemostats, Microbial Ecology, 46(1), 1-11, July 2003
• Musarrat J, Hashsham SA, Customized cDNA microarray for expression profiling of environmentally important genes of Pseudomonas stutzeri strain KC, Teratogenesis, Carcinogenesis, and Mutagenesis, 23 Suppl 1, 283-94, 2003
• Callister, SJ., Ayala-del-Rio H.L., and S.A. Hashsham, Quantification of a single population in a mixed community using a laser integrated microarray scanner, Environmental Engineering and Science, 2(4), 247-253, 2003
• Hashsham, S.A., Rouillard, J-M., Gao, X., S. Callister, Cole, J., Denef, V.J., Tsoi, T.V., Wibbenmeyer, J., Gulari, E., and J. Tiedje, Highly Parallel Microbial Detection using In situ Synthesized Flexible Biochips, Proceedings of the 103rd General Meeting of the American Society of Microbiology, May 18-22, Washington D.C., 2003
• Denef, V.J, Tsoi, T.V., Park J., Zhang, H., Gao, X., Wang, X., Wibbenmeyer, J.A., Hashsham, S.A., and Tiedje, J.M., Outlining genome-wide carbon source-specifci metabolic networks in Burkholderia sp. nov, Proceedings of the 103rd General Meeting of the American Society of Microbiology, Washington D.C., 2003
• Sun B, Griffin BM, Ayala-del-Rio HL, Hashsham SA, Tiedje JM, Microbial dehalorespiration with 1,1,1-trichloroethane, Science, 298(5595), 1023-5, November 2002
• Dolhopf, S.L., S.A. Hashsham, and J.M. Tiedje., Interpreting 16S rDNA T-RFLP Data: Application of Self-Organizing Maps and Principal Component Analysis to Describe Community Dynamics and Convergence, Microbial Ecology, 42(4), 495-505, November 2001
• Dollhopf SL, Hashsham SA, Dazzo FB, Hickey RF, Criddle CS, Tiedje JM, The impact of fermentative organisms on carbon flow in methanogenic systems under constant low-substrate conditions, Applied Microbiology and Biotechnology, 56(3-4), 531-8, August 2001
• Musarrat, J., J. Larabee, C. Criddle, and S.A. Hashsham, Monitoring the abundance of mRNA transcripts associated with carbon tetrachloride dechlorination in Pseudomonas stutzeri strain KC under different environmental conditions using DNA microarray, Proceedings of the 101st General Meeting of the American Society for Microbiology., Orlando, Florida, Ma, 20 May 2001
• Callister, S.J., H.L. Ayala, and S.A. Hashsham, Quantification of Pseudomonas cepacia G4 in a dense mixed microbial community using a dual laser microarray scanner, . Proceedings of the 101st General Meeting of the American Society for Microbiology. Orlando, Florida, 20 May 2001
• Denef, V.J., C.A. Kachel, J.L.M. Rodrigues, S.A. Hashsham, T.V. Tsoi, and J.M.Tiedje, DNA microarray approach to monitor engineered mixed PCB-degrading communities, Proceedings of the 101st General Meeting of the American Society for Microbiology. Orlando, Florida, 20 May 2001
• Tiedje, J., A. Fernandez, S. Hashsham, S, Dolhopf, F. Dazzo, R. Hickey, and C. Criddle, Stability, persistence and resilience in anaerobic reactors; a community unveiled, Advances in Water and Wastewater Treatment Technologies: Molecular Technology, Nutrient Removal, Sludge production and Environmental Health, Ed. T. Matsuo, K. Hanaki, S. Takizawa, and H. Satoh, Elsevier Amsterdom, vol. 1, pp. 13-20, 2001
• S.A. Hashsham, T.L. Marsh, S, Dolhopf, A.S. Fernandez, F.B. Dazzo, R.F. Hickey, C. Criddle, and J.M. Tiedje., Relating function and communty structure of complex microbial systems using neural networks, In Advances in Water and Wastewater Treatment Technologies: Molecular Technology, Nutrient Removal, Sludge production and Environmental Health. Ed. T. Matsuo, K. Hanaki, S. Takizawa, and H. Satoh. Elsevier, Amsterdom., Vol 1, pp. 67-77, 2001
• Hashsham SA, Fernandez AS, Dollhopf SL, Dazzo FB, Hickey RF, Tiedje JM, Criddle CS, Parallel processing of substrate correlates with greater functional stability in methanogenic bioreactor communities perturbed by glucose, Applied and Environmental Microbiology, 66(9), 4050-7, September 2000
• Fernandez AS, Hashsham SA, Dollhopf SL, Raskin L, Glagoleva O, Dazzo FB, Hickey RF, Criddle CS, Tiedje JM, Flexible community structure correlates with stable community function in methanogenic bioreactor communities perturbed by glucose, Applied and Environmental Microbiology, 66(9), 4058-67, September 2000
• Hashsham SA, Freedman DL, Enhanced biotransformation of carbon tetrachloride by Acetobacterium woodii upon addition of hydroxocobalamin and fructose, Applied and Environmental Microbiology, 65(10), 4537-42, October 1999
• Hashsham S., T. Marsh, A. Fernandez, L. Raskin, S. Seston, F. Dazzo, R. Hickey, C. Criddle, and J. Tiedje, Microbial Ecology of Anaerobic Digesters: Stability and Persistence during Shock Loads, . Proceedings of the 8th International Symposium on Microbial Ecology. Halifax, Canada, 9 Aug 1998
• Hashsham S., T. Marsh, S. Seston, F. Dazzo, R. Hickey, C. Criddle, and J. Tiedje., Perturbed Anaerobic Microbial Communities: Structure and Stability, Proceedings of the 98th General Meeting of the American Society for Microbiology. Atlanta, Georgia, 14 May 1997
• Freedman, D.L., and S. Hashsham., Enhanced biotransformation of carbon tetrachloride by an anaerobic enrichment culture, Proceedings of the Fourth International Symposium, In Situ and On-Site Bioremediation, sponsored by Battelle, New Orleans, Louisiana, 28 Apr 1997
• Hashsham, S.A., R. Scholze, and D.L.Freedman., Cobalamin-enhanced anaerobic biotransformation of carbon tetrachloride, Environmental Science and Technology, Vol. 29, pp. 2856-2863, 1995
• Hashsham, S.A., Wick, L.M., Rouillard, J-M., Gulari, E., and J.M. Tiedje, Potential of dna microarrays for developing parallel detection tools (PDTs) for microorganisms relevant to biodefense and related research needs, Biosensors and Bioelectronics (In Press)
• Hashsham, S.A. S. Callister, and M. Tijdens., Oligonucleotide probe design for mixed microbial community microarrays and other applications and important considerations for data analysis, in Molecular Microbial Ecology Manual (MMEM), 2nd edition, 2004. Elsevier Science, The Netherlands.