Dr. John Hawdon

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George Washington University
School of Medicine and Health Sciences
Microbiology and Tropical Medicine
Associate Professor
Professional Headshot of John Hawdon

Mailing Address

705 Ross Hall
2300 Eye St. NW
Washington, District of Columbia 20037
United States

Contact Information

Phone: (202) 994-2652
Fax: (202) 994-2913
mtmjmh@gwumc.edu
http://www.gwumc.edu/microbiology/faculty/hawdon.htm

Qualifications

Ph.D., University of Pennsylvania, Parasitology, 1991.
B.S., Pennsylvania State University, Animal Science, 1981.

Expertise and Research Interests

My research interests focus around the hookworm infectious process. The developmentally arrested infective stage receives a host-specific signal that re-initiates suspended developmental pathways that culminate in development of the adult parasite. I am investigating the molecular events of this activation to parasitism in the framework of dauer recovery in the free-living nematode Caenorhabditis elegans. Using a novel in vitro activation assay I developed, I have confirmed that hookworm infective larvae use the same signaling pathways during activation that C. elegans dauers use during recovery. I am continuing to dissect the pathways in hookworm larvae by identifying the host-specific signal and its receptor, and the molecules involved in the transduction of this signal and the molecular events of development.

I am also interested in the population genetics of natural populations of hookworms infecting humans in China. I have an ongoing NIH- funded project examining the genetic structure of hookworm populations and molecular variation in candidate vaccine antigens. These studies have demonstrated the presence of genetically isolated populations of the human hookworm Necator americanus in China. Variation in these populations could complicate the design of recombinant vaccines and effective drug-based control strategies. Future studies will investigate the effect of transmission patterns on the intra- and interhost population genetics of hookworms in China.

Keywords

COS Keywords:

Human Population and Genetics, Infectious Diseases or Agents, Molecular Biology, Nematology, Parasitology, Undergraduate Education.

Additional Terms:

Caenorhabditis Elegans, Infectious Disease, Insulin Signaling, Molecular Biology, Nematode, Parasitology, Population Genetics.

Memberships

American Association of Veterinary Parasitologists
American Society of Parasitologists
American Society of Tropical Medicine and Hygiene
Helminthological Society of Washington

Patents

Hookworm Vaccine, Patent Number: 7303752 B2, 2007, Institution, United States of America.

Funding Received

  • National Institutes of Health (NIH): Role of Insulin Signaling in Hookworm Infection, 2007 to 2011.

Publications

  • Abubucker, S., Martin, J., Yin, Y. (2008) The canine hookworm genome: Analysis and classification of Ancylostoma caninum survey sequences., Molecular and Biochemical Parasitology, 157, 187-192
  • Brand, A., Varghese, G., Majewski, W., Hawdon, J.M. (2005) Identification of a DAF-7 ortholog from the hookworm Ancylostoma caninum., International Journal for Parasitology, 35 (14), 1489-98
  • Hawdon, J.M. (2005) Introduction of Gerhard A. Schad as the recipient of the Clark P. Read Mentor Award, Journal of Parasitology, 91 (6), 1253-125
  • Delaney, A., Williamson, A., Brand, A., Ashcom, J., Varghese, G., Goud, G.N., Hawdon, J.M., Cloning and Characterisation of An Aspartyl Protease Inhibitor (API-1) From Ancylostoma Hookworms, International Journal for Parasitology, 35(3), 303-313, Mar 2005
  • Li, T. H., Guo, X. R., Xue, J., Hu, L., Qiang, H. Q., Xue, H. C., Bin, Z., HAWDON, J. M. and Xiao, S. H., Comparison of mitochondrial cytochrome oxidase 1 DNA sequences from Necator americanus hookworms maintained for 100 generations in golden hamsters (Mesocricetus auratus) and hookworms from natural hum, Acta Tropica, 92(1), 72-75, September 2004
  • Zhan B, Wang Y, Liu Y, Williamson A, Loukas A, Hawdon JM, Xue HC, Xiao SH, Hotez PJ, Ac-SAA-1, an immunodominant 16 kDa surface-associated antigen of infective larvae and adults of Ancylostoma caninum, International Journal for Parasitology, 34(9), 1037-45, August 2004 Abstract
  • Brand A, Hawdon JM, Phosphoinositide-3-OH-kinase inhibitor LY294002 prevents activation of Ancylostoma caninum and Ancylostoma ceylanicum third-stage infective larvae, International Journal for Parasitology, 34(8), 909-14, July 2004 Abstract
  • Loukas A, Bethony JM, Williamson AL, Goud GN, Mendez S, Zhan B, Hawdon JM, Elena Bottazzi M, Brindley PJ, Hotez PJ, Vaccination of dogs with a recombinant cysteine protease from the intestine of canine hookworms diminishes the fecundity and growth of worms, The Journal of Infectious Diseases, 189(10), 1952-61, May 2004 Abstract
  • Goud GN, Zhan B, Ghosh K, Loukas A, Hawdon J, Dobardzic A, Deumic V, Liu S, Dobardzic R, Zook BC, Jin Q, Liu Y, Hoffman L, Chung-Debose S, Patel R, Mendez S, Hotez PJ, Cloning, yeast expression, isolation, and vaccine testing of recombinant Ancylostoma-secreted protein (ASP)-1 and ASP-2 from Ancylostoma ceylanicum, The Journal of Infectious Diseases, 189(5), 919-29, March 2004 Abstract
  • Hotez PJ, Zhan B, Bethony JM, Loukas A, Williamson A, Goud GN, Hawdon JM, Dobardzic A, Dobardzic R, Ghosh K, Bottazzi ME, Mendez S, Zook B, Wang Y, Liu S, Essiet-Gibson I, Chung-Debose S, Xiao S, Knox D, Meagher M, Inan M, Correa-Oliveira R, Vilk P, Sheph, Progress in the development of a recombinant vaccine for human hookworm disease: the Human Hookworm Vaccine Initiative, International Journal for Parasitology, 33(11), 1245-58, September 2003 Abstract
  • Zhan B, Liu Y, Badamchian M, Williamson A, Feng J, Loukas A, Hawdon JM, Hotez PJ, Molecular characterisation of the Ancylostoma-secreted protein family from the adult stage of Ancylostoma caninum, International Journal for Parasitology, 33(9), 897-907, August 2003 Abstract
  • Hotez PJ, Ashcom J, Zhan B, Bethony J, Loukas A, Hawdon J, Wang Y, Jin Q, Jones KC, Dobardzic A, Dobardzic R, Bolden J, Essiet I, Brandt W, Russell PK, Zook BC, Howard B, Chacon M, Effect of vaccination with a recombinant fusion protein encoding an astacinlike metalloprotease (MTP-1) secreted by host-stimulated Ancylostoma caninum third-stage infective larvae, The Journal of Parasitology, 89(4), 853-5, August 2003 Abstract
  • Hawdon JM, Datu B, The second messenger cyclic GMP mediates activation in Ancylostoma caninum infective larvae, International Journal for Parasitology, 33(8), 787-93, July 2003 Abstract
  • Hawdon JM, Datu B, Crowell M, Molecular cloning of a novel multidomain Kunitz-type proteinase inhibitor from the hookworm Ancylostoma caninum, The Journal of Parasitology, 89(2), 402-7, April 2003 Abstract
  • Basavaraju S, Zhan B, Kennedy MW, Liu Y, Hawdon J, Hotez PJ, Ac-FAR-1, a 20 kDa fatty acid- and retinol-binding protein secreted by adult Ancylostoma caninum hookworms: gene transcription pattern, ligand binding properties and structural characteris, Molecular and Biochemical Parasitology, 126(1), 63-71, January 2003 Abstract
  • Li TH, Guo XR, Hu L, Xiao SH, Xue HC, Hawdon J, [Polymorphism analysis of cytochrome C oxidase 1 (COI) gene in Necator americanus collected from 5 provinces in China], Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases., 21(4), 210-3, 2003 Abstract
  • Gandhi NS, Jizhang C, Khoshnood K, Fuying X, Shanwen L, Yaoruo L, Bin Z, Haechou X, Chongjin T, Yan W, Wensen W, Dungxing H, Chong C, Shuhua X, Hawdon JM, Hotez PJ, Epidemiology of Necator americanus hookworm infections in Xiulongkan Village, Hainan Province, China: high prevalence and intensity among middle-aged and elderly residents, Journal of Parasitology, 87(4), 739-43, August 2001 Abstract
  • Hawdon JM, Li T, Zhan B, Blouin MS, Genetic structure of populations of the human hookworm, Necator americanus, in China, Molecular Ecology, 10(6), 1433-7, June 2001 Abstract
  • Qiang S, Bin Z, Shu-hua X, Zheng F, Hotez P, Hawdon JM, Variation between ASP-1 molecules from Ancylostoma caninum in China and the United States, Journal of Parasitology, 86(1), 181-5, February 2000 Abstract
  • Tissenbaum HA, Hawdon J, Perregaux M, Hotez P, Guarente L, Ruvkun G, A common muscarinic pathway for diapause recovery in the distantly related nematode species Caenorhabditis elegans and Ancylostoma caninum, Proceedings of the National Academy of Sciences (USA), 97(1), 460-5, 2000 Abstract
  • Hawdon JM, Introduction of Professor Peter J. Hotez as the Henry Baldwin Ward Medalist for 1999, Journal of Parasitology, 85(6), 1000, December 1999 Abstract
  • Hawdon JM, Narasimhan S, Hotez PJ, Ancylostoma secreted protein 2: cloning and characterization of a second member of a family of nematode secreted proteins from Ancylostoma caninum, Molecular and Biochemical Parasitology, 99(2), 149-65, 1999 Abstract
  • Bin Z, Hawdon J, Qiang S, Hainan R, Huiqing Q, Wei H, Shu-Hua X, Tiehua L, Xing G, Zheng F, Hotez P, Ancylostoma secreted protein 1 (ASP-1) homologues in human hookworms, Molecular and Biochemical Parasitology, 98(1), 143-9, 1999 Abstract
  • Hawdon JM, Differentiation between the human hookworms Ancylostoma duodenale and Necator americanus using PCR-RFLP, Journal of Parasitology, 82(4), 642-7, August 1996 Abstract
  • Hawdon JM, Jones BF, Hoffman DR, Hotez PJ, Cloning and characterization of Ancylostoma-secreted protein. A novel protein associated with the transition to parasitism by infective hookworm larvae, Journal of Biological Chemistry, 271(12), 6672-8, 1996 Abstract
  • Hawdon JM, Jones BF, Perregaux MA, Hotez PJ, Ancylostoma caninum: metalloprotease release coincides with activation of infective larvae in vitro, Experimental Parasitology, 80(2), 205-11, March 1995 Abstract
  • Hawdon JM, Jones BF, Hotez PJ, Cloning and characterization of a cDNA encoding the catalytic subunit of a cAMP-dependent protein kinase from Ancylostoma caninum third-stage infective larvae, Molecular and Biochemical Parasitology, 69(1), 127-30, January 1995 Abstract
  • Hawdon JM, Schad GA, Ancylostoma caninum: glutathione stimulates feeding in third-stage larvae by a sulfhydryl-independent mechanism, Experimental Parasitology, 77(4), 489-91, December 1993 Abstract
  • Hawdon JM, Volk SW, Rose R, Pritchard DI, Behnke JM, Schad GA, Observations on the feeding behaviour of parasitic third-stage hookworm larvae, Parasitology, 106 ( Pt 2), 163-9, February 1993 Abstract
  • Hawdon JM, Volk SW, Pritchard DI, Schad GA, Resumption of feeding in vitro by hookworm third-stage larvae: a comparative study, Journal of Parasitology, 78(6), 1036-40, December 1992 Abstract
  • Hawdon JM, Schad GA, Ancylostoma caninum: reduced glutathione stimulates feeding by third-stage infective larvae, Experimental Parasitology, 75(1), 40-6, August 1992 Abstract
  • Hawdon JM, Schad GA, Albumin and a dialyzable serum factor stimulate feeding in vitro by third-stage larvae of the canine hookworm Ancylostoma caninum, Journal of Parasitology, 77(4), 587-91, August 1991 Abstract
  • Hawdon JM, Schad GA, Serum-stimulated feeding in vitro by third-stage infective larvae of the canine hookworm Ancylostoma caninum, Journal of Parasitology, 76(3), 394-8, June 1990 Abstract

Profile Details

Last Updated: 9/17/2008

COS Expertise ID #973335
Reference this profile directly: http://myprofile.cos.com/jhawdon