Prof. Juergen Wiegel

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University of Georgia
College of Arts and Sciences
Microbiology & Biochemistry
Distinguished Research ProfessorAppointed: 2008
University of Georgia
College of Arts and Sciences
Microbiology
ProfessorAppointed: 1982
University of Georgia
College of Arts and Sciences
Ecology
Adjunct Professor
University of Georgia
College of Arts and Sciences
Biochemistry and Molecular Biology
Adjunct Professor
Professional Headshot of Juergen Wiegel

Mailing Address

Biological Sciences Building
University of Georgia
Rm. 211-212C
Athens, Georgia 30602
United States

Contact Information

Phone: (706) 542-2651
Fax: (706) 542-2651
jwiegel@uga.edu

Qualifications

Dr. Sci (Dr. habil), University of Goettingen, Microbiology, 1983.
Ph.D. (Dr. rer. nat.), University of Goettingen, Microbiology, 1973.
M.Sc. (Dipl. chem), University of Goettingen, Organic Chemistry, 1969.
B.S., University of Goettingen, Chemistry, 1968.
Certificate, Landwirtschaftskammer Hannover, Horticulture, 1960.

Expertise and Research Interests

My current research interests and areas of expertise include taxonomy and the systematics of thermophilic and extreme thermophilic anaerobic bacteria as well as their physiological and biochemical properties especially in respect to biotechnological application. Presently we are investigating especially novel alkalithermophilic anaerobes including extreme halophilic alkalithermophiles, which represent the novel Order Natranaerobiales in the Phylum Firmicutes. An area of interest is the biogeography and biogeochemistry of thermophiles, i.e., how microorganisms influence the geochemistry and mineralogy of geothermal environments and vice versa. (for updated list of publications see http://www.uga.edu/mib/jwiegel.htm)

We investigate thermostable enzymes from anaerobic thermophiles which included mainly in the past hemicellulases, cellulases,and proteolytic enzymes. Presently we are working on the most thermostable and thermophilic lipases from Thermosyntropha lipolytica as well as in cooperation with Prof Shao (China) on the regulation of the ethanol-DH in Thermoanaerobacter. We are interested in temperature effects on physiological properties including but not limited to growth rate, growth range (we developed a specific theory on bacteria growing over a wide temperature span), enzyme stability, spore heat stability, and bioenergetic of the halophilic alkalithermophiles

Presently, we are not anymore working actively on the anaerobic degradation of halogenated aromatic compounds (such as poly chlorinated biphenyl (PCB's and 2,4-dichlorophenols) or on the enzymes catalyzing the reversible co-factor independent anaerobic decarboxylation/carboxylation of aromatic compounds

Two sideline: 1)(although presently no ongoing research project by a graduate student or postdoc) is the old research area of N2-fixation by facultative chemolithoautotrophic and microaerophilic N2-fixing bacteria. This includes the taxonomic systematic, physiological and biochemical properties as well as plant microbe interactions of Xanthobacter with patty rice.
2) anaerobic mesophilic microorganisms from acid mine drainage passed through a constructed wetland model system

Other Expertise

Horticulture: 4 years of experience in Germany prior to study of chemistry and microbiology.

Future Research

Ongoing research deals with:
- The diversity of anaerobic thermophiles, with the focus on alkalithermophiles and halophilic alkalithermophiles
- Anaerobic hemicellulose degradation at elevated temperatures
- The elucidation of the bioenergetics of the haloalkalithermophiles
-developing a genetic system for Natranaerobius using our previously developed sytem for Thermoanaerobacterium
- biogeography of Thermoanaerobacter uzonii in the Uzon Caldera (Kamchatka, Far East Russia)

Industrial Relevance

Many of our novel thermophilic isolates have quite a lot of potential for industrial applications either as source for genes for artificial evolution of novel enzymes or directly as biocatalyst.
(proteases, lipases, hydrolytic enzymes for biomass degradation).
The highly thermophilic lipase has a potential in the position modification of lipids and synthesis of specific lipids and synthesis of higher fatty acid esters.

Our research on the influence of environmental factors on the anaerobic (reductive) dehalogenation of PCBs and chlorophenols are important for development of bioremediation processes and/or for estimating the recalcitrance of the compounds in a given environment. Our data can be used to help to decide whether bioremediation should be done or whether the environment will slowly remove the compounds (passive bioremediation).

Keywords

COS Keywords:

Bacteriology, Biochemistry, Biodiversity, Biogeochemistry, Bioremediation, Biotechnology, Enzymes, Microbiology, Plant Taxonomy, Systematics.

Additional Terms:

Alkaliphiles, Anaerobic Dechlorination, Anaerobic Microbiology, Anaerobic Phenol Degradation, Biochemistry, Bioconversion, Biodiversity, Biogeochemistry, Biomass Conversion, Bioremediation, Biotechnology, Biotechnology, Cellulose, Deductive Dechlorination, Degradation, Environment, Extreme Thermophilic Anaerobe, Extreme Thermophilic Organism, Haloaromatic Compound, Hemicellulose, Hemicellulose Degradation, Microaerophilic N2 Fixation, Microaerophilic N2-fixation (xanthobactes), Microbiology, N2 Fixation, Nitrogen, Organism, PCB, PCB-degradation, Phenol, Phylogeny, Polychlorinated Biphenyl, Reductive Dechlorination, Renewable Resource, Stressed Environment, Systematics, Taxonomy, Thermophilic Anaerobe, Thermophilic Organism, Thermophilicity, Waste, Waste Treatment, Xylanase.

Languages

(Reading, Writing, Speaking)

English: (Fluent, Fluent, Fluent)
German: (Fluent, Fluent, Fluent)

Memberships

American Society for Microbiology
German Society for Microbiology
ISME, International Society for Microbial Ecology
ISSLR, International Society for Salt Lake Research
International Society for Extremophiles
International Society for Microbial Ecology
Society for Environmental Toxicology and Chemistry
Society for General Microbiology
Society for Industrial Microbiology

Honors and Awards

2008, Distinguished Research Professor, University of Georgia
2007, Inaugural Outstanding Teaching award for Freshman Seminar, University of Georgia
2007, AAAS- Elected Fellow, AmercianAssociationfor the Advancement of Science
1997, P.R. Edward Award for Outstanding Service and Accomplishments in Microbiology, Southeastern Branch of American Society for Microbiology
Elected Fellow of the American Academy for Micrbiology, American Society for Microbiology
Thermoanaerobacter wiegelii, IJSEB-journal
Bergey's Manual Medal 2007, Bergey's Trust

Previous Positions

1985-1990, Associate Professor, University of Georgia
1982-1985, Visiting Associate Professor, University of Georgia
1969-1984, Research Scientist, University of Goettingen, Germany

Patents

M. Seyfried, J. Wiegel, and G. Whited (2004) Enzymes which dehydrate glycerol. (International patent filed, Patent Number: United States Patent6803218., 2004, Institution, United States of America.
Anaerobic thermophilic culture system, Patent Number: 4292406, 1981, Institution-owned, United States of America.
Anaerobic thermophilic culture, Patent Number: 4292407, 1981, Institution-owned, United States of America.

Funding Received

  • United States Department of Energy (DOE): Conference "Incredible Anaerobes: From Physiology to Genomics to Fuels". March 2/3. $ 8,000 (DEFG0207ER64352), 2007 to 2008.
  • Air Force Office of Scientific Research (AFOSR): Life t the Edge: Elucidation of the Mechanisms how triple extremophiles , the halo-alkalithermophiles produce ATP and cope with the combined stress of high salt, alkaline pH and elevated temperature ($ 450,000) (2.92 yrs), 2007 to 2009.
  • JGI/DOE: JGI CSP 2007 Elucidation of how a triple extremophilic, anaerobic haloalkalithermophile, 'Natronanaerobium thermophilum' can cope with combined stresses of high salt concentrations at alkaline pH and elevated temperatures. (genome sequence service provide, 2006 to .
  • JGI /DOE: Subject: JGI CSP 2007 Genome sequence assisted molecular and biochemical characterization of a possible candidate for the ancient microbial assisted Banded Iron formation Thermolithobacter ferrireducens, gen.nov., sp. nov., a chemolithoautotroph, 2006 to .
  • NSF: 2006-2008 Bioenergetics in'Natronanaerobium alkaliphilum' JW/NM-L6, a member of the anaerobic halo-alkalithermophiles, a novel group of triple extremophiles $ 96,93, 2006 to 2008.
  • National Science Foundation (NSF): 2004-2007 MIP NSF 0348180 Microbial interactions and processes: Diversity, function, and biogeochemical consequences of chemolithoautotrophic archaea in Nevada hot springs (Co-PI with Chuanlun Zhang (corresponding PI), and Christopher Romanek from SREL) $, 2004 to 2007.
  • MicrobialObservatory NSF 0238407: Microbial Observatoryjavascript:document.update_form.submit(), 999,964, 2003 to 2007.
  • US-Egypt Cooperative research NSF_INT 0211100: Biodiversity of aerobic and anaerobic alkalithermophiles & their industrial enzymes form alkaline lakes and hot springs, 25,000, 2002 to 2004.
  • PI Cady, PSU: Kamchatkabiogeochemical researchProgram, 30,000, 2002 to 2003.
  • United States Department of Energy (DOE): Novel reversible phenolic carboxylase family shared by members of the dmains Bacteria and Archaea, 303,000, 2001 to 2004.
  • ODP=Ocean Drilling Program: Leg 201 and Postcruise Research, 42,718 + 22,140, 2001 to 2004.
  • ERDA, (University of Georgia): High Level Waste (Co-PI L. Shimkets), 100,000, 2000 to 2000.
  • CDRF: Interdisciplinary workshop on biogeochemistry research program Kamchatka, 34,540, 2000 to 2001.
  • Diversa: thermophilic clostridia and related alkalithermophiles, 70,500, 2000 to 2002.
  • SGER: Novel extremophiles from highly radioactive waste tanks, 100,000, 1998 to 1999.
  • Enzyme Bio system: GI-isomerase, 2,000, 1998 to 1998.
  • DuPont: Glycerol utilizing anaerobes, 60,000, 1998 to 1999.
  • National Science Foundation (NSF): Thermophiles '96 International conference and Workshop, 10,000, 1996 to 1996.
  • United States Department of Energy (DOE): Thermophiles '96, 17,500, 1996 to 1996.
  • Genecor: Microorganisms for novelprocesses, 87,000, 1996 to 1997.
  • General Electric (GE): Anaerobic PCB degradation, 186,591, 1994 to 1997.
  • Development of principals to obtain improved thermostable glucose isomerase (GI) with the desired acidic pH-range, Research Foundation, University of Georgia, 1995-1996, $31,327
  • DE-FG05-95ER20199, Hemicellulases from anaerobic thermophiles and development of a genetic system for the ethanologenic Thermoanaerobacter ethanolicus for an augmentation of its hemicellulases, Department of Energy, 1995-1996, $91,000
  • Novel microorganisms, Genencor, $100,000, 1994-1996
  • Desulfitobacterium dehalogenans, DuPont, $20,000, 1994-1996
  • Training grant for graduate students, US. Naval Research, $39,241, 1994-1995
  • DE-FG09-86ER-13614, Anaerobic alkali-thermophiles, a novel group of extremely fast growing organisms able to grow at 60 degrees C, and and pH 11, Department of Energy, $77,000, 1993-1995
  • DE-FG09-89ER-14059, The hemicellulases from the ethanologenic thermophile Thermoanaerobacter ethanolicus, Department of Energy, $246000, 1992-1995
  • Characterization of selected bacteria and enzymes involoved in the sequential, anaerobic degradation of 2,4-dichlorophenol, $348,241, 1991-1994
  • DE-FG09-89ER-14059, The hemicellulases from the ethanologenic thermophile Thermoanaerobacter ethanolicus, Department of Energy, $29,000, 1993
  • Equipment grant for anaerobic degradation of chlorinated aromatic compounds, Georgia Environmental Technology Consortium (GETC), $80,500, 1993
  • DE-FG09-89ER-14059, The hemicellulases from the ethanologenic thermophile Thermoanaerobacter ethanolicus, Department of Energy, $156,000, 1989-1992
  • DE-FG09-86ER-13164, Microbiology and physiology of anaerobic fermentation, Department of Energy, $30,000, 1989-1992

Publications

  • Breitenstein A, Wiegel J, Haertig C, Weiss N, Andreesen JR, Lechner U, Reclassification of Clostridium hydroxybenzoicum as Sedimentibacter hydroxybenzoicus gen. nov., comb. nov., and description of Sedimentibacter saalensis sp. nov., International Journal of Systematic and Evolutionary Microbiology, 52(Pt 3), 801-7, May 2002 Abstract
  • Phillips RW, Wiegel J, Berry CJ, Fliermans C, Peacock AD, White DC, Shimkets LJ, Kineococcus radiotolerans sp. nov., a radiation-resistant, gram-positive bacterium, International Journal of Systematic and Evolutionary Microbiology, 52(Pt 3), 933-8, May 2002 Abstract
  • Canganella F, Kuk SU, Morgan HW, Wiegel J, Clostridium thermobutyricum: Growth studies and stimulation of butyrate formation by acetate supplementation, Microbiological Research, 157, 149-156, 2002
  • Seyfried D, Lyon D, Rainey FA, Wiegel J, Caloramator viterbensis sp. nov., a novel thermophilic glycerol-fermenting bacterium isolated from a hot spring in Italy, Int J Syst Evol Microbiol, 52, 1177-1184, 2002
  • Breitenstein A, Wiegel J, Hartig C, WeiB N, Andreesen J.R, Lechner U., Description of Sedimentibacter saalensis ZF2 gen. nov. and reclassification of Clostridium hydroxygenzoicum JW/Z-1T as Sedimentibacter hydroxybenzoicus JW/Z-1T gen. nov., comb. nov, Int J Syst Evol Microbiol, 52, 801-807 published on, 2001
  • Mai V, Wiegel J., Advances in development of a genetic system for Thermoanaerobacterium spp.: expression of genes encoding hydrolytic enzymes, development of a second shuttle vector, and integration of genes into the c, Applied and Environmental Microbiology, 66(11), 4817-21, November 2000
  • Byrer DE, Rainey FA, Wiegel J, Novel strains of Moorella thermoacetica form unusually heat-resistant spores, Archives of Microbiology, 174(5), 334-9, November 2000
  • Watson J, Matsui GY, Leaphart A, Rainey FA, Wiegel J, Lovell CR, Reductively debrominating strains of Propionigenium maris from burrows of bromophenol producing marine infauna, Intern J of Syst and Evol Microbiol, 50, 1035-1042, 2000
  • Mai, V, Wiegel J, Lorenz W., Cloning, sequencing, and characterization of the bifunctional xylose-arabinosidase from the anaerobic thermophile Thermoanaerobacter ethanolicus, Gene, 247, 137-143, 2000
  • Byrer DE, Rainey FR, Wiegel J, Novel strains of Moorella thermoacetica form unusually heat resistant spores, Arch Microbiol, 174, 334-339; DOI 10.1000, 2000
  • Mai V, Wiegel J., Advances in the development of a genetic system for Thermoanaerobacterium: Expression of genes encoding hydrolytic enzymes, development of a second shuttle vector and integration of genes into the chr, Applied and Environmental Microbiology, 66, 4817-4821, 2000
  • Sridhar J, Eiteman MA, Wiegel JW, Elucidation of enzymes in fermentation pathways used by Clostridium thermosuccinogenes growing on inulin, Applied and Environmental Microbiology, 66(1), 246-51, Jan 2000
  • Shridhar J, Eiteman MA, Wiegel J, Elucidation of enzymes in the fermentation pathways used by Clostridium thermosuccinogenes growing on inulin, Applied and Environmental Microbiology, 66, 246-251, 2000
  • Huang J, He Z, Wiegel J., Cloning, characterization, and expression of a novel gene encoding a reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum, Journal of Bacteriology, 181(16), 5119-22, August 1999
  • Wiegel J, Zhang X, Wu Q., Anaerobic dehalogenation of hydroxylated polychlorinated biphenyls by Desulfitobacterium dehalogenans, Applied and Environmental Microbiology, 65(5), 2217-21, May 1999
  • Wu Q, Bedard D, Wiegel J., 2,6-dibromobiphenyl primes extensive dechlorination of Aroclor 1260 in contaminated sediment at 8 to 30 C by stimulating growth of PCB dehalogenating microorganisms, Environmental Science and Technology, 33, 595-602, 1999
  • Wiegel J, Zhang X, Wu Q., Anaerobic dehalogenation of hydroxylated PCBs by Desulfitobacterium dehalogenans, Applied and Environmental Microbiology, 65, 2217-2221, 1999
  • Huang J, He Z, Wiegel J., Cloning, characterization, and expression of a novel gene encoding a reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum, Journal of Bacteriology, 181, 5119-5122, 1999
  • Canganella F, Wiegel J., Cultivation of Clostridium thermobutyricum in a rotary fermentor system, Journal of Industrial Microbiology and Biotechnology, 24, 7-13, 1999
  • Wiegel J, Anaerobic alkalithermophiles, a novel group of extremophiles., Extremophiles, 2(3), 257-67, August 1998 Abstract
  • Mackiewicz M, Wiegel J., Comparison of energy and growth yields for Desulfitobacterium dehalogenans when utilizing chlorophenol and various traditional electron acceptors, Applied and Environmental Microbiology, 64, 342-355, 1998
  • Wu Q, Wiegel J., Two anaerobic polychlorinated biphenyl-dehalogenating enrichments that exhibit different para-dechlorination specificities, Applied and Environmental Microbiology, 63(12), 4826-32, December 1997
  • Wu Q, Bedard D.L, Wiegel J., Temperature determines the pattern of anaerobic microbial dechlorination of Aroclor 1260 primed by 2,3,4,6-tetrachlorobiphenyl in Woods Pond sediment, Applied and Environmental Microbiology, 63(12), 4818-25, December 1997
  • Slobodkin A, Reysenbach A.L, Mayer F, Wiegel J., Isolation and characterization of the homoacetogenic thermophilic bacterium Moorella glycerini sp. nov, International Journal of Systematic Bacteriology, 47(4), 969-74, October 1997
  • Lorenz W.W, Wiegel J., Isolation, analysis, and expression of two genes from Thermoanaerobacterium sp. strain JW/SL YS485: a beta-xylosidase and a novel acetyl xylan esterase with cephalosporin C deacetylase activity, Journal of Bacteriology, 179(17), 5436-41, September 1997
  • Slobodkin A.I, Wiegel J., Fe(III) as an electron acceptor for H2 oxidation in thermophilic anaerobic enrichment cultures from geothermal areas, Extremophiles, 1(2), 106-9, May 1997
  • Slobodkin A, Reysenbach A.L, Strutz N, Dreier M, Wiegel J., Thermoterrabacterium ferrireducens gen. nov., sp. nov., a thermophilic anaerobic dissimilatory Fe(III)-reducing bacterium from a continental hot spring, International Journal of Systematic Bacteriology, 47(2), 541-7, April 1997
  • Mai V, Lorenz WW, Wiegel J, Transformation of Thermoanaerobacteirum spec. strain JW/SL-YS 485 with plasmid pIKM1 conferring kanamycin resistance, FEMS Letters, 148, 163-167, 1997
  • Slobodkin A, Wiegel J, Fe(III) as an electron acceptor for H2 oxidation in thermophilic anaerobic enrichment cultures from geothermal areas, Extremophiles, 2, 106-109, 1997
  • Slobodkin A, Reysenbach AL, Wiegel J, Isolation and characterization of the homoacetogenic thermophile Moorella glycerini sp. nov, Int J Syst Bacteriol, 47, 969-974, 1997
  • Brill J, Wiegel J., Differentiation between sporeforming and asporogenenic bacteria by a PCR and Southern hybridization based method, Journal of Microbiological Methods, 31, 29-36, 1997
  • Lorenz W, Wiegel J., Isolation, analysis, and expression of two genes from Thermoanaerobacterium sp. strain JW/SL-YS 485: a novel acetyl xylan esterase with cephalosporin-C deacetylase activity and a beta-xylosidase, Journal of Bacteriology, 179, 5436-5441, 1997
  • Wu Q, Bedard D, Wiegel J., Effect of incubation temperature on the route of microbial reductive dechlorination of 2,3,4,6-tetrachlorobiphenyl in PCB-contaminated and PCB-free freshwater sediments, Applied and Environmental Microbiology, 63, 2836-2843, 1997
  • Wu Q, Bedard D, Wiegel J., Influence of temperature on primed microbial dechlorination of PBCs (Aroclor 1260) in freshwater sediments, Applied and Environmental Microbiology, 63, 4818-4825, 1997
  • Wu Q, Wiegel J., Two anaerobic polychlorinated biphenyl-dehalogenating enrichments that exhibit different para-dechlorination specificities, Applied and Environmental Microbiology, 63, 4826-4832, 1997
  • Boothe DDH, Rogers JE, Wiegel J, Reductive dechlorination of chlorophenols in slurries of low-organic-carbon marine sediments and subsurface soils, Appl Microbiol Biotechnol, 47, 742-748, 1997
  • Slobodkin A, Reysenbach A.L, Struty N, Dreier M, Wiegel J., Thermoterrabacterium ferrireducens, gen. nov., sp. nov., a thermophilic anaerobic dissimilatory Fe(III)-reducing bacterium from a continental hot spring, Int J Syst Bacteriol, 47, 541-547, 1997
  • Liu S.Y, Wiegel J, Gherardini FC, Purification and cloning of a thermostable xylose (glucose) isomerase with an acidic pH optimum from Thermoanaerobacterium strain JW/SL-YS 489, Journal of Bacteriology, 178(20), 5938-45, October 1996
  • Svetlitshnyi V, Rainey F, Wiegel J., Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, alkalitolerant, thermophilic bacterium utilizing short- and long-chain fatty acids in syntrophic coculture with a methanogenic a, International Journal of Systematic Bacteriology, 46(4), 1131-7, October 1996
  • Engle M, Li Y, Rainey F, DeBlois S, Mai V, Reichert A, Mayer F, Messner P, Wiegel J., Thermobrachium celere gen. nov., sp. nov., a rapidly growing thermophilic, alkalitolerant, and proteolytic obligate anaerobe, International Journal of Systematic Bacteriology, 46(4), 1025-33, October 1996
  • He Z, Wiegel J., Purification and characterization of an oxygen-sensitive, reversible 3,4-dihydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum, Journal of Bacteriology, 178(12), 3539-43, June 1996
  • Liu SY, Gherardini FC, Matuschek M, Bahl H, Wiegel J, Cloning, sequencing, and expression of the gene encoding a large S-layer-associated endoxylanase from Thermoanaerobacterium sp. strain JW/SL-YS 485 in Escherichia coli, Journal of Bacteriology, 178(6), 1539-47, March 1996
  • Liu SY, Rainey FA, Morgan HW, Mayer F, Wiegel J, Thermoanaerobacterium aotearoense, sp. nov., a slightly acidophilic, anaerobic thermophile isolated from various hot springs in New Zealand and emendation of the genus Thermoanaerobacterium, Int J Syst Bacteriol, 46, 388-396, 1996
  • Liu YS, Gherardini FC, Matuschek M, Bahl H, Wiegel J, Cloning, sequencing, and expression of the gene encoding a large S-layer-associated endoxylanase from Thermoanaerobacterium sp. strain JW/SL-YS 485 in Escherichia coli, Journal of Bacteriology, 178, 1539-1547, 1996
  • Rainey FA, Wiegel J, 16S rDNA sequence analysis confirms the close relationship between the genera Xanthobacter, Azorhizobium, and Aquabacter and reveals a lack of phylogenetic coherence among the species of the genus Xan, Int Syst Bacteriol, 46, 907-910, 1996
  • Engle M, Li Y, Rainey F, DeBlois S, Mai V, Reichert A, Mayer F, Messmer P, Wiegel J, Thermobrachium celere, gen. nov., sp. nov., a fast growing thermophilic, alkalitolerant, and proteolytic obligate anaerobe, Int J Syst Bacteriol, 46, 1025-1033, 1996
  • He Z, Wiegel J., Purification and characterization of an oxygen-sensitive, reversible 3,4-dihydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum, Journal of Bacteriology, 178, 3539-3543, 1996
  • Liu SY, Wiegel J, Gherardini FC, Purification and cloning of a thermostable xylose (glucose)isomerase with an acidic pH optimum form Thermoanaerobacterium sp. strain JW/SL-YS 489, Journal of Bacteriology, 178, 5938-5945, 1996
  • Svetlitshnyi V, Rainey F, Wiegel J, Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, organoheterotrophic, alkalitolerant thermophile utilizing short- and long chain fatty acids in syntrophic coculture with a alkal, Int J Syst Bacteriol, 46, 1131-1137, 1996
  • Cook GM, Russel JB, Reichert A, Wiegel J, The intracellular pH of Clostridium paradoxum, an anaerobic alkaliphilic, and thermophilic bacterium, Applied and Environmental Microbiology, 62, 4576-4579, 1996
  • Wu Q, Bedard D, Wiegel J, Influence of incubation temperature on the microbial reductive dechlorination of 2,3,4,6-tetrachlorobiphenyl in two freshwater sediments, Applied and Environmental Microbiology, 62, 4174-4179, 1996
  • Engle M, Li Y, Woese C, Wiegel J., Isolation and characterization of a novel alkalitolerant thermophile, Anaerobranca horikoshii gen. nov., sp. nov, International Journal of Systematic Bacteriology, 45(3), 454-61, July 1995
  • He Z, Wiegel J., Purification and characterization of an oxygen-sensitive reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum, European Journal of Biochemistry, 229(1), 77-82, 1 Apr 1995
  • Shao W, Wiegel J., Purification and characterization of two thermostable acetyl xylan esterases from Thermoanaerobacterium sp. strain JW/SL-YS485, Applied and Environmental Microbiology, 61(2), 729-33, February 1995
  • Utkin I, Dalton DD, Wiegel J, Specificity of reductive dechlorination of substituted orthochlorophenols by Desulfitobacterium dehalogenans JW/IU-DC1, Applied and Environmental Microbiology, 61, 346-351, 1995
  • Shao W, DeBlois S, Wiegel J, A high-molecular weight, cell-associated xylanase isolated from exponentially growing Thermoanaerobacterium sp. strain JW/SL-YS485, Applied and Environmental Microbiology, 61, 937-940, 1995
  • Shao W, Wiegel J., Purification and characterization of two thermostable acetyl xylan esterases from Thermoanaerobacterium sp. strain JW/SL-YS485, Applied and Environmental Microbiology, 61, 729-733, 1995
  • Shao W, Obi SKC, Puls J, Wiegel J, Purification and characterization of the alpha-glucuronidase from Thermoanaerobacterium sp. strain JW/SL-YS485, an important enzyme for the utilization of substituted xylans, Applied and Environmental Microbiology, 61, 1077-1081, 1995
  • DeBlois S, Wiegel J., Celluloytic vestiges of the xylanase activity in a new strictly xylanolytic thermophile Clostridium sp, Biotechnology Letters, 17, 89-94, 1995
  • He Z, Wiegel J., Purification and characterization of an oxygen-sensitive reversible 4-hydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum, European Journal of Biochemistry, 229, 77-82, 1995
  • Engle M, Li Y, Woese C, Wiegel J., Isolation and characterization of a novel alkalitolerant thermophile, Anaerobranca horikoshii gen. nov. sp. nov, Int J Syst Bacteriol, 45, 454-461, 1995
  • Utkin I, Dalton D.D, Wiegel J., Specificity of reductive dehalogenation of substituted ortho-chlorophenols by Desulfitobacterium dehalogenans JW/IU-DC1 [published erratum appears in Appl Environ Microbiol 1995 Apr;61(4):1677], Applied and Environmental Microbiology, 61(1), 346-51, January 1995
  • Utkin I, Woese C, Wiegel J., Isolation and characterization of Desulfitobacterium dehalogenans gen. nov., sp. nov., an anaerobic bacterium which reductively dechlorinates chlorophenolic compounds, International Journal of Systematic Bacteriology, 44(4), 612-9, October 1994
  • Zhang X, Mandelco L, Wiegel J., Clostridium hydroxybenzoicum sp. nov., an amino acid-utilizing, hydroxybenzoate-decarboxylating bacterium isolated from methanogenic freshwater pond sediment [published erratum appears in Int J Syst B, International Journal of Systematic Bacteriology, 44(2), 214-22, April 1994
  • Li Y, Engle M, Weiss N, Mandelco L, Wiegel J., Clostridium thermoalcaliphilum sp. nov., an anaerobic and thermotolerant facultative alkaliphile, International Journal of Systematic Bacteriology, 44(1), 111-8, January 1994
  • Li Y, Engle M, Mandelco L, Wiegel J., Clostridium thermoalcaliphilum sp. nov., an anaerobic and thermotolerant facultative alkaliphile, Int J Syst Bacteriol, 44, 111-118, 1994
  • Li Y, Engle M, Mandelco L, Wiegel J., Clostridium thermoalcaliphilum sp. nov., an anaerobic and thermotolerant facultative alkaliphile, Int J Syst Bacteriol, 44, 111-118, 1994
  • Zhang X, Mandelco L, Wiegel J., Clostridium hydroxybenzoicum sp. nov.: an amino acid utilizing, hydroxybenzoate decarboxylating bacterium isolated from a methanogenic freshwater pond sediment, Int J Syst Bacteriol, 44, 214-222, 1994
  • Utkin I, Woese C, Wiegel J., Isolation and characterization of Desulfitobacterium dehalogenans gen. nov. sp. nov., an anaerobic bacterium which reductively dechlorinates chlorophenolic compounds, Int J Syst Bacteriol, 44, 612-619, 1994
  • Wiegel J, Potentials of acetogenic bacteria for industrial processes, Acetogenesis (Drake, ed) Chapman and Hall, NY, 484-504, 1994
  • Peterandel R, Canganella F, Holzenburg A, Wiegel J., Induction and regeneration of autoplasts from Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200, Applied and Environmental Microbiology, 59, 3498-3501, 1993
  • Reding HK, Wiegel J, Motility and chemotaxis of a XANTHOBACTER rice isolate, Journal of General Microbiology, 139, 815-820, 1993
  • Li Y, Mandelco L, Wiegel J., Isolation and characterization of a moderately thermophilic anaerobic alkaliphile, Clostridium paradoxum, sp.nov, Int J Syst Bacteriol, 43, 450-460, 1993
  • Zhang X, Wiegel J., The anaerobic degradation of 3-chloro-4-hydroxybenzoate in freshwater sediment proceeds via either chlorophenol or hydroxybenzoate to phenol and subsequently to benzoate, Applied and Environmental Microbiology, 58(11), 3580-5, November 1992
  • Shao W, Wiegel J., Purification and characterization of a thermostable beta-xylosidase from Thermoanaerobacter ethanolicus, Journal of Bacteriology, 174(18), 5848-53, September 1992
  • Reding HK, Croes GL, Dijkhuizen L, Wiegel J, Emendation of Xanthobacter flavus as a motile species [published erratum appears in Int J Syst Bacteriol 1992 Jul; 42(3):512], International Journal of Systematic Bacteriology, 42(2), 309-11, April 1992
  • Bryant F, Wiegel J, Ljungdahl LG, Comparison of alcohol dehydrogenases from wild type and mutant strain, JW200 Fe4, of Thermoanaerobacter ethanolicus, Appl Microbiol Biotech, 37, 490-495, 1992
  • Zhang X, Wiegel J., The anaerobic degradation of 3-chloro-4-hydroxybenzoate in freshwater sediments proceeds either via chlorophenol or hydroxybenzoate to phenol before it is carboxylated to benzoate, Applied and Environmental Microbiology, 58, 3580-3585, 1992
  • Shao W, Wiegel J., Purification and characterization of a thermostable beta-xylosidase from Thermoanaerobacter ethanolicus, Journal of Bacteriology, 174, 5848-5853, 1992
  • Reding HK, Croes GLM, Dijkhuizen L, Wiegel J, Emendation of Xanthobacter flavus as a motile species, Int J Syst Bacteriology, 42, 309-311, 1992
  • Hale DD, Rogers JE, Wiegel J, Environmental factors correlating to dichlorophenol dechlorination in anoxic freshwater sediments, Environ. Toxicol. Chem., 10, 1255-1265, 1991
  • Reding HK, Hartel PG, Wiegel J, Effect of Xanthobacter, isolated and characterized from rice roots on growth of wetland rice, Plant and Soil, 138, 221-229, 1991
  • Peteranderl R, Shotts EB Jr, Wiegel J, Stability of antibiotics under growth conditions for thermophilic anaerobes, Applied and Environmental Microbiology, 56(6), 1981-3, June 1990
  • Zhang X, Wiegel J., Sequential anaerobic degradation of 2,4-dichlorophenol in freshwater sediments, Applied and Environmental Microbiology, 56(4), 1119-27, April 1990
  • Zhang X, Morgan V, Wiegel J., Conversion of 13C-1-phenol to 14C-4 benzoate, an intermediate step in the anaerobic degradation of chlorophenols, FEMS Microbiology Letters, 67, 63-66, 1990
  • Hale DD, Rogers JE, Wiegel J, Reductive dechlorination of dichlorophenols by non-adapted and adapted microbial communities in pond sediments, Microbial Ecology, 20, 185-196, 1990
  • Zhang X, Wiegel J., Isolation and partial characterization of a para hydroxybenzoate transforming Clostridium spec. producing phenol as the final transformation product, Microbial Ecology, 20, 103-121, 1990
  • Peteranderl R, Shotts EB Jr, Wiegel J., Stability of antibiotics at growth conditions of thermophilic anaerobes, Applied and Environmental Microbiology, 56, 1981-1983, 1990
  • Kohring S, Wiegel J, Mayer F., Subunit composition and glycosidic activities of the cellulase complex from Clostridium thermocellum JW 20, Applied and Environmental Microbiology, 56, 3798-3804, 1990
  • Wiegel J, Temperature spans for growth: A hypothesis and discussion, FEMS Microbiology Reviews, 75, 155-170, 1990
  • Kohring GW, Zhang XM, Wiegel J, Anaerobic dechlorination of 2,4-dichlorophenol in freshwater sediments in the presence of sulfate, Applied and Environmental Microbiology, 55(10), 2735-7, October 1989
  • Wiegel J, Kuk S, Kohring GW, Clostridium thermobutyricum, spec. nov. a moderate thermophile isolated from a cellulolytic culture producing butyrate as major product, Int J Syst Bacteriol, 39, 199-204, 1989
  • Bateson M, Wiegel J, Ward DM, Comparative analysis of 16S ribosomal RNA sequences of thermophilic fermentative bacteria isolated from hot springs cyanobacterial mats, Syst Appl Microbiol, 12, 1-7, 1989
  • Kohring GW, Rogers JE, Wiegel J, Anaerobic biodegradation of 2,4-dichlorophenol in freshwater lake sediments at different temperatures, Applied and Environmental Microbiology, 55, 348-353, 1989
  • Freier D, Wiegel J, Gottschalk G., The formation of ethanol and butanol by Clostridium thermosaccharolyticum at neutral pH, Biotechnology Letters, 11, 831-836, 1989
  • Kohring GW, Zhang X, Wiegel J, Anaerobic transformation of 2,4-dichlorophenol in freshwater sediments in the presence of sulfate, Applied and Environmental Microbiology, 56, 2735-2737, 1989
  • Bryant FO, Wiegel J and Ljungdahl LG, Purification and properties of primary and secondary alcohol dehydrogenases from Thermoanaerobacter ethanolicus, Applied and Environmental Microbiology, 54, 460-465, 1988
  • Freier D, Mothershed ChP, Wiegel J, Clostridium thermocellum characterization of strain JW20, Applied and Environmental Microbiology, 54, 104-111, 1988
  • Patel BK, Morgan HW, Wiegel J, Daniel RM, Isolation of an extremely thermophilic chemoorganotrophic anaerobe similar to Dictyoglomus thermophilium from New Zealand hot springs, Arch Microbiol, 147, 21-24, 1987
  • Jenni B, Aragno M, Wiegel J., Numerical analysis and DNA-DNA-hybridization studies on Xanthobacter and emendation of Xanthobacter flavus, System Appl Microbiol, 9, 247-253, 1987
  • Kneifel H, Stetter KO, Andreesen J, Wiegel J, Koenig H, Schobert SM, Distribution of polyamines in representative species of archaebacteria, Syst Appl Microbiol, 7, 241-245, 1986
  • Wiegel J, Ljungdahl LG, The importance of thermophilic bacteria in biotechnology, CRS-Reviews in Biotechnology, 3, 39-107, 1986
  • Ljungdahl LG, Carreira LH, Garrison RJ, Rabek NE, Gunter LF, Wiegel J, CMA-Manufacture (II): Improved bacterial strain for acetate production, National Technical Information Service, Springfield, Virginia, 1986
  • Ljungdahl LG, Carreira LH, Garrison R, Rabek N and Wiegel J, Comparison of three thermophilic homoacetogenic bacteria from Ca-Mg acetate production, Biotechnology and Bioengineering Symposium Series, 15, 207-223, 1985
  • Wiegel J, Mothershed ChP, Puls J, Differences in xylan degradation by various non-cellulolytic thermophilic anaerobes and Clostridium thermocellum, Applied and Environmental Microbiology, 49, 656-659, 1985
  • Wiegel J, Leucine biosynthesis in Alcaligenes eutrophus H16. Influence of amino acid additions on the formation of active alpha-isopropylmalate synthase and alpha-hydroxy acid synthase, Arch Microbiol, 142, 194-199, 1985
  • Wiegel J, Dykstra M., Clostridium thermocellum: Adhesion and sporulation while adhered to cellulose and hemicellulose, European Journal of Applied Microbiology and Biotechnology, 20, 59-65, 1984
  • Allison MJ, Baetz AL, Wiegel J, Regulation of carbon flow in leucine biosynthesis in Bacteroides, Applied and Environmental Microbiology, 48, 1111-1117, 1984
  • Korner HU, Gottschalk D, Wiegel J, Puls J, Analysis of the degradation pattern of oligomers and polymers from lignocelluloses, Analytical Chimica Acta, 163, 55-66, 1984
  • Carreira LH, Wiegel J, Ljungdahl LG, Production of ethanol from biopolymers by anaerobic, thermophilic and extreme thermophilic bacteria. I. Regulation of carbohydrate utilization in mutants of Thermoanaerobacter ethanolicus, Biotechnology and Bioengineering Symposium Series, 13, 183-191, 1983
  • Wiegel J, Carreira LH, Mothershed ChP, Puls J, Production of ethanol from biopolymers by anaerobic, thermophilic and extreme thermophilic bacteria. II. Thermoanaerobacter ethanolicus JW200 and its mutants in batch cultures and resting cell experim, Biotechnology and Bioengineering Symposium Series, 13, 193-205, 1983
  • Ljungdahl LG, Petterson B, Eriksson KE, Wiegel J, A yellow affinity substance involved in the cellulolytic system of Clostridium thermocellum, Current Microbiol, 9, 195-199, 1983
  • Clark JE, Ragsdale SW, Ljungdahl LG, Wiegel J, Levels of enzymes involved in the synthesis of acetate from CO2 in Clostridium thermoautotrophicum, Journal of Bacteriology, 151, 507-509, 1982
  • Wiegel J, Quandt L., Determination of the Gram type using the reaction between polymyxin B and lipopolysaccharides of the outer cell wall of whole bacteria, Journal of General Microbiology, 128, 2261-2270, 1982
  • Wiegel J, Kleiner D., Survey of ammonium (methylammonium-transport of aerobic N2-fixing bacteria; the special case of Rhizobium, FEMS Letters, 15, 61-63, 1982
  • Kandler O, Konig H, Wiegel J, Claus D., Occurrence of poly-D-glutamic acid and poly-L-glutamine in the genera Xanthobacter, Flexithrix, Sporosarcina, and Planococcus, System Appl Microbiol, 4, 34-41, 1982
  • Wiegel J, Ljungdahl LG, Thermoanaerobacter ethanolicus gen. nov., spec. nov., a new, extreme thermophilic, anaerobic bacterium, Arch Microbiol, 128, 343-348, 1981
  • Wiegel J, Braun M, Gottschalk G., Clostridium thermoautotrophicum specius novum, a thermophile producing acetate from molecular hydrogen and carbon dioxide, Current Microbiology, 5, 255-26, 1981
  • Wiegel J, alpha-Isopropylmalate synthase as a marker for the leucine biosynthetic pathway in several clostridia and in Bacteroides fragilis, Arch Microbiol, 130, 385-390, 1981
  • Wiegel J, Distinction between the Gram reaction and the Gram type of bacteria, Int J Syst Bacteriol, 31, 88, 1981
  • Wiegel J, Ljungdahl L.G, Rawson JR, Isolation from soil and properties of the extreme thermophile Clostridium thermohydrosulfuricum, Journal of Bacteriology, 139, 800-810, 1979
  • Wiegel J, Mn++-specific reactivation of EDTA inactivated alpha-isopropylmalate synthase from Alcaligenes eutrophus H16, Biochemical and Biophysical Research Communications, 82, 907-912, 1978
  • Wiegel J, Mayer F., Isolation of lipopolysaccharides and the effect of polymyxin B on the outer membrane of Corynebacterium autotrophicum, Arch Microbiol, 118, 67-69, 1978
  • Wiegel J, Wilke D, Baumgarten J, Opitz R, Schlegel H., Transfer of the nitrogen-fixing hydrogen bacterium Corynebacterium autotrophicum Baumgarten et al. to Xanthobacter gen. nov, Int J Syst Bacteriol, 28, 573-581, 1978
  • Wiegel J, Schlegel HG, alpha-Isopropylmalate synthase from Alcaligenes eutrophus H16, Arch Microbiol, 112, 239-246, 1977
  • Wiegel J, Schlegel HG, alpha-Isopropylmalate synthase from Alcaligenes eutrophus H16, Arch Microbiol, 112, 247-254, 1977
  • Wiegel J, Schlegel HG, alpha-Isopropylmalate synthase from Alcaligenes eutrophus H16, Arch Microbiol, 114, 203-210, 1977
  • Wiegel J, Schlegel HG, Leucine biosynthesis: Effect of branched-chain amino acids and threonine on alpha-isopropylmalate synthase activity from aerobic and anaerobic microorganisms, Biochemical Systematics and Ecology, 5, 169-176, 1977
  • Wiegel J, Schlegel HG, Enrichment and isolation of nitrogen fixing hydrogen bacteria, Arch Microbiol, 107, 139-142, 1976
  • Engles M, Li Y, Rainey F, DeBlois S, Mai V, Reichert A, Mayer, F, Messmer P, Wiegel J., Thermobrachium celer, gen. nov., sp. nov., a fast growing thermophilic, alkalitolerant and proteolytic obligate anaerobe, Int J Syst Bacteriol, 46(October issue), in press

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