Keith R. Jerome

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University of Washington
School of Medicine
Laboratory Medicine
Associate Professor
Fred Hutchinson Cancer Research Center
Program in Infectious Diseases
Assistant Member
University of Washington
School of Medicine
Microbiology
Adjunct Associate Professor
Professional Headshot of Keith R. Jerome

Mailing Address

1100 Fairview Ave. N.
D3-100
Seattle, Washington 98109
United States

Contact Information

Phone: (206) 667-6793
kjerome@fhcrc.org

Qualifications

M.D., Duke University, 1993.
Ph.D., Duke University, Immunology, 1992.
B.S., Georgetown College, Chemistry, 1985.

Expertise and Research Interests

My research interests focus on killing mechanisms used by cytotoxic T lymphocytes, the interplay of these killing mechanisms with viral or cellular anti-apoptosis genes, the transduction of negative signals to CTL by virus-infected cells, and the effect of these interactions on immune control of viral infections and malignancy. Understanding these immune evasion strategies may help explain the incomplete control of chronic viral infections by the immune system, the lack of success of current vaccination approaches to many viruses, and the inability of the immune system to control many tumors.

Clinically, I serve as director of the University of Washington molecular virology laboratory, and consult regarding all aspects of virology testing. Specific areas of interest include real time PCR, automation of nucleic acid extraction, and diagnosis of disease caused by herpesviruses, enterovirus, JC and BK viruses, parvovirus B19, and hepatitis viruses.

Keywords

COS Keywords:

Apoptosis, Cellular Immunology, Clinical Research or Studies, Hepatitis, Herpes, Immunology, Infectious Diseases or Agents, Laboratory Practice or Procedure, Microbiology, Molecular Pathology, Pathology, Real-Time Computing, Sexually Transmitted Diseases, Signal Transduction, Venereal Diseases, Virology.

Additional Terms:

Anergy, Apoptosis, Immunology, Virology.

Funding Received

  • National Foundation for Infectious Diseases: Does HSV inhibition of apoptosis influence the severity of clinical disease?, May 1, 1999 to Apr 30, 2000.
  • National Foundation for Infectious Diseases: Herpes simplex virus interference with cytotoxic T lymphocyte killing mechanisms, Jul 1, 1996 to Jun 30, 1997.
  • National Institutes of Health: Herpes simplex virus inhibition of CTL-induced apoptosis, Jan 1, 1998 to Dec 30, 2001.
  • National Institutes of Health (NIH): Inhibition of CTL killing by HSV, Apr 1, 2001 to Mar 31, 2006.
  • National Institutes of Health (NIH): Mechanisms of inactivation of T cells by HSV, 2006 to 2008.
  • National Institutes of Health (NIH): Inactivation of CTL by HSV-infected cells, 2004 to 2007.

Publications

  • Lin CH, Sloan DD, Dang CH, Wagner T, Cabrera AJ, Tobin NH, Frenkel LM, Jerome KR (Sep 2008) Assessment of mitochondrial toxicity by analysis of mitochondrial protein expression in mononuclear cells., Cytometry. Part B, Clinical cytometry Abstract
  • Hoffman NG, Cook L, Atienza EE, Limaye AP, Jerome KR (Aug 2008) Marked variability of BK virus load measurement using quantitative real-time PCR among commonly used assays., Journal of clinical microbiology, 46 (8), 2671-80 Abstract
  • Zahariadis G, Wagner MJ, Doepker RC, Maciejko JM, Crider CM, Jerome KR, Smiley JR (Jul 2008) Cell-type-specific tyrosine phosphorylation of the herpes simplex virus tegument protein VP11/12 encoded by gene UL46., Journal of virology, 82 (13), 6098-108 Abstract
  • Jerome KR (May 2008) Viral modulation of T-cell receptor signaling., Journal of virology, 82 (9), 4194-204 Abstract
  • Aubert M, Chen Z, Lang R, Dang CH, Fowler C, Sloan DD, Jerome KR (Jan 2008) The antiapoptotic herpes simplex virus glycoprotein J localizes to multiple cellular organelles and induces reactive oxygen species formation., Journal of virology, 82 (2), 617-29 Abstract
  • Sloan DD, Jerome KR (Nov 2007) Herpes simplex virus remodels T-cell receptor signaling, resulting in p38-dependent selective synthesis of interleukin-10., Journal of virology, 81 (22), 12504-14 Abstract
  • Kim HN, Harrington RD, Shuhart MC, Cook L, Morishima C, Jerome KR, Wang CC (Oct 2007) Hepatitis C virus activation in HIV-infected patients initiating highly active antiretroviral therapy., AIDS patient care and STDs, 21 (10), 718-23 Abstract
  • Castor J, Cook L, Corey L, Jerome KR (Aug 2007) Rapid detection directly from patient serum samples of human cytomegalovirus UL97 mutations conferring ganciclovir resistance., Journal of clinical microbiology, 45 (8), 2681-3 Abstract
  • Han JY, Sloan DD, Aubert M, Miller SA, Dang CH, Jerome KR (Mar 2007) Apoptosis and antigen receptor function in T and B cells following exposure to herpes simplex virus., Virology, 359 (2), 253-63 Abstract
  • Cook L, Sullivan K, Krantz EM, Bagabag A, Jerome KR (Nov 2006) Multiplex real-time reverse transcription-PCR assay for determination of hepatitis C virus genotypes., Journal of clinical microbiology, 44 (11), 4149-56 Abstract
  • Stüve O, Marra CM, Bar-Or A, Niino M, Cravens PD, Cepok S, Frohman EM, Phillips JT, Arendt G, Jerome KR, Cook L, Grand'Maison F, Hemmer B, Monson NL, Racke MK (Oct 2006) Altered CD4+/CD8+ T-cell ratios in cerebrospinal fluid of natalizumab-treated patients with multiple sclerosis., Archives of neurology, 63 (10), 1383-7 Abstract
  • Stüve O, Marra CM, Jerome KR, Cook L, Cravens PD, Cepok S, Frohman EM, Phillips JT, Arendt G, Hemmer B, Monson NL, Racke MK (May 2006) Immune surveillance in multiple sclerosis patients treated with natalizumab., Annals of neurology, 59 (5), 743-7 Abstract
  • Sloan DD, Han JY, Sandifer TK, Stewart M, Hinz AJ, Yoon M, Johnson DC, Spear PG, Jerome KR (Feb 2006) Inhibition of TCR signaling by herpes simplex virus., Journal of immunology (Baltimore, Md. : 1950), 176 (3), 1825-33 Abstract
  • Aubert M, Krantz EM, Jerome KR (2006) Herpes simplex virus genes Us3, Us5, and Us12 differentially regulate cytotoxic T lymphocyte-induced cytotoxicity., Viral immunology, 19 (3), 391-408 Abstract
  • Cook L, Jerome KR (Jul 2005) Mass spectrometry for genotyping hepatitis C virus: a promising new approach., Clinical chemistry, 51 (7), 1091-2 Abstract
  • Lai KK, Cook L, Krantz EM, Corey L, Jerome KR (Jul 2005) Calibration curves for real-time PCR., Clinical chemistry, 51 (7), 1132-6 Abstract
  • Carpenter PA, Lowder J, Johnston L, Frangoul H, Khoury H, Parker P, Jerome KR, McCune JS, Storer B, Martin P, Appelbaum F, Abonour R, Westervelt P, Anasetti C (Jun 2005) A phase II multicenter study of visilizumab, humanized anti-CD3 antibody, to treat steroid-refractory acute graft-versus-host disease., Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation., 11 (6), 465-71 Abstract
  • Limaye AP, Smith KD, Cook L, Groom DA, Hunt NC, Jerome KR, Boeckh M (Mar 2005) Polyomavirus nephropathy in native kidneys of non-renal transplant recipients., American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 5 (3), 614-20 Abstract
  • Cook L, Ng KW, Bagabag A, Corey L, Jerome KR (Sep 2004) Use of the MagNA pure LC automated nucleic acid extraction system followed by real-time reverse transcription-PCR for ultrasensitive quantitation of hepatitis C virus RNA., Journal of Clinical Microbiology, 42 (9), 4130-6 Abstract
  • Jerome KR, Gretch DR (Mar 2004) Laboratory approaches to the diagnosis of hepatitis C virus infection., Minerva Gastroenterologica E Dietologica, 50 (1), 9-20 Abstract
  • Nesbitt SE, Cook L, Jerome KR (Mar 2004) Cytomegalovirus quantitation by real-time PCR is unaffected by delayed separation of plasma from whole blood., Journal of Clinical Microbiology, 42 (3), 1296-7 Abstract
  • Jerome KR, Corey L, The danger Within, New England Journal of Medicine, 350, 4-5, 2004
  • Jerome KR, Sloan DD, Aubert M, Measurement of CTL-induced cytotoxicity: The caspase 3 assay, Apoptosis : an International Journal On Programmed Cell Death, 8(6), 563-71, December 2003 Abstract
  • DD Sloan, G Zahariadis, CM Posavad, N Pate, S Kussick, and KR Jerome, Cytotoxic T lymphocytes are inactivated by HSV-infected cells expressing a viral protein kinase, Journal of Immunology, 171, 6733-6741, December 2003
  • Aubert M, Jerome KR, Apoptosis prevention as a mechanism of immune evasion, International Reviews of Immunology, 22(5-6), 361-71, 2003 Abstract
  • Zahariadis G, Jerome KR, Corey L, Herpes simplex virus-associated sepsis in a previously infected immunocompetent adult, Annals of Internal Medicine, 139(2), 153-4, July 2003 Abstract
  • Lai KK, Cook L, Wendt S, Corey L, Jerome KR, Evaluation of real-time PCR versus PCR with liquid-phase hybridization for detection of enterovirus RNA in cerebrospinal fluid, Journal of Clinical Microbiology, 41(7), 3133-41, July 2003 Abstract
  • Kadambi PV, Josephson MA, Williams J, Corey L, Jerome KR, Meehan SM, Limaye AP, Treatment of refractory BK virus-associated nephropathy with cidofovir, American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons., 3(2), 186-91, February 2003 Abstract
  • Jerome KR, Sloan DD, Aubert M, Measuring T-cell-mediated cytotoxicity using antibody to activated caspase 3, Nature Medicine, 9(1), 4-5, January 2003 Abstract
  • Musey L, Ding Y, Cao J, Lee J, Galloway C, Yuen A, Jerome KR, McElrath MJ, Ontogeny and specificities of mucosal and blood human immunodeficiency virus type 1-specific CD8(+) cytotoxic T lymphocytes, Journal of Virology, 77(1), 291-300, January 2003 Abstract
  • Jerome KR, Huang ML, Wald A, Selke S, Corey L, Quantitative stability of DNA after extended storage of clinical specimens as determined by real-time PCR, Journal of Clinical Microbiology, 40(7), 2609-11, July 2002 Abstract
  • Jerome KR, Chen Z, Lang R, Torres MR, Hofmeister J, Smith S, Fox R, Froelich CJ, Corey L, HSV and glycoprotein J inhibit caspase activation and apoptosis induced by granzyme B or Fas, Journal of Immunology (baltimore, Md. : 1950), 167(7), 3928-35, October 2001 Abstract
  • CM Murry, KR Jerome, DD Richenbach, Fatal parvovirus myocarditis in a 5 year old girl, Human Pathology, 32, 342-345, 2001
  • AP Limaye, KR Jerome, CS Kuhr, J Ferrenberg, ML Huang, CL Davis, L Corey, and CL Marsh, Quantitation of BK virus load in serum for the diagnosis of BK virus-associated nephropathy in renal transplant patients, Journal of Infectious Diseases, 183, 1669-1672, 2001
  • Jerome KR, Fox R, Chen Z, Sarkar P, Corey L, Inhibition of apoptosis by primary isolates of herpes simplex virus, Archives of Virology, 146(11), 2219-25, 2001 Abstract
  • Loeb KR, Jerome KR, Goddard J, Huang ML, Cent A, Corey L, High-throughput quantitative analysis of hepatitis B virus DNA in serum using the TaqMan fluorogenic detection system, Hepatology, 32(3), 626-9, September 2000 Abstract
  • Jerome KR, Vallan C, and Jaggi R, Caveats in the use of the TUNEL assay for diagnosis of graft-versus-host disease, Pathology, 32, 186-190, August 2000
  • Jerome KR and Chen Z, Apoptosis in HL-60 cells and topoisomerase I in vivo, Archives of Pathology and Laboratory Medicine, 124, 802-804, June 2000
  • Jerome KR, Fox R, Chen Z, Sears AE, Lee Hy, Corey L, Herpes simplex virus inhibits apoptosis through the action of two genes, Us5 and Us3, Journal of Virology, 73(11), 8950-7, November 1999 Abstract
  • Jerome KR, Conyers SJ, Hansen DA, Zebala JA, Keratinocyte apoptosis following bone marrow transplantation: evidence for CTL-dependent and -independent pathways, Bone Marrow Transplantation, 22(4), 359-66, August 1998 Abstract
  • Jerome KR, Tait JF, Koelle DM, Corey L, Herpes simplex virus type 1 renders infected cells resistant to cytotoxic T-lymphocyte-induced apoptosis, Journal of Virology, 72(1), 436-41, Jan 1998 Abstract
  • Jerome KR, Kirk AD, Pecher G, Ferguson WW, Finn OJ, A survivor of breast cancer with immunity to MUC-1 mucin, and lactational mastitis, Cancer Immunology, Immunotherapy, 43(6), 355-60, January 1997 Abstract
  • Finn OJ, Jerome KR, Henderson RA, Pecher G, Domenech N, Magarian-Blander J, Barratt-Boyes SM, MUC-1 epithelial tumor mucin-based immunity and cancer vaccines, Immunological Reviews, 145, 61-89, June 1995 Abstract
  • Ioannides CG, Fisk B, Jerome KR, Irimura T, Wharton JT, Finn OJ, Cytotoxic T cells from ovarian malignant tumors can recognize polymorphic epithelial mucin core peptides, Journal of Immunology, 151(7), 3693-703, October 1993 Abstract
  • Jerome KR, Domenech N, Finn OJ, Tumor-specific cytotoxic T cell clones from patients with breast and pancreatic adenocarcinoma recognize EBV-immortalized B cells transfected with polymorphic epithelial mucin complementary DNA, Journal of Immunology, 151(3), 1654-62, August 1993 Abstract
  • Jerome KR, Bu D, Finn OJ, Expression of tumor-associated epitopes on Epstein-Barr virus-immortalized B-cells and Burkitt's lymphomas transfected with epithelial mucin complementary DNA, Cancer Research, 52(21), 5985-90, November 1992 Abstract
  • Jerome KR, Barnd DL, Bendt KM, Boyer CM, Taylor-Papadimitriou J, McKenzie IF, Bast RC Jr, Finn OJ, Cytotoxic T-lymphocytes derived from patients with breast adenocarcinoma recognize an epitope present on the protein core of a mucin molecule preferentially expressed by malignant cells, Cancer Research, 51(11), 2908-16, June 1991 Abstract

Profile Details

Last Updated: 12/31/2008

COS Expertise ID #425101
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