Duncan Charles Krause

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University of Georgia
College of Arts and Sciences
Microbiology
ProfessorAppointed: 1996

Mailing Address

Department of Microbiology
019 Riverbend South Building
220 Riverbend Road
University of Georgia
Athens, Georgia 30602
United States

Contact Information

Phone: (706) 542-2671
Fax: (706) 542-3804
dkrause@uga.edu

Qualifications

Ph.D., University of North Carolina, Microbiology, 1982.
B.S., Auburn University, Microbiology, 1978.

Expertise and Research Interests

My basic research interests are in molecular aspects of host - parasite interactions, focusing on Mycoplasma pneumoniae. M. pneumoniae is a pathogen of the human respiratory tract, causing bronchitis and atypical or 'walking' pneumonia. This cell wall-less prokaryote is the leading cause of pneumonia in older children and young adults. M. pneumoniae colonizes the mucosal epithelium of the bronchial passages via a differentiated terminal structure called the attachment organelle, which also functions in gliding motility and cell division. We study the architecture, assembly, regulation, and function of this terminal organelle. Ultrastructural and molecular approaches are used to dissect the adherence apparatus in order to define more clearly the identities and roles of its component proteins in attachment organelle function. By characterizing a battery of mutants defective in attachment organelle assembly we have begun to reconstruct the assembly process. Transposon-based approaches are being utilized to manipulate the mycoplasma genome in order to probe this process further, including structure-function analyses of attachment organelle proteins. In addition, fluorescent protein fusions are being employed to examine the assembly process in real time. Finally, gliding motility yields satellite colonies when wild-type M. pneumoniae is grown under the appropriate culture conditions. Non-motile mutants fail to produce satellite growth under such conditions. We are utilizing a combination of approaches to dissect the mechanism of gliding motility and assess the contribution of gliding to M. pneumoniae virulence.

Other Expertise

Academic Experience:
Medical bacteriology
Rickettsial diseases

Divisional Officer for American Society for Microbiology
Scientific Program Chair, 14th Congress of the International Organization for Mycoplasmology, 2002
Meeting Chair, 15th Congressof the International Organization for Mycoplasmology, 2004
Member, Board of Directors, International Organization for Mycoplasmology, 2000-2004

Keywords

COS Keywords:

Bacteriology, Cell Cycle, Communicable Diseases, Cytoskeleton, Gene Cloning, Gene Regulation, Infectious Diseases Or Agents, Microbiology, Pathogenesis, Proteins and Macromolecules, Respiratory Diseases.

Additional Terms:

Cell Development, Cytadherence, Cytoskeleton, Gene Regulation Protein Phosphorylation, Gliding Motility, Mycoplasma, Pathogenesis, Phase Variation.

Memberships

American Society for Microbiology
International Organization for Mycoplasmology

Honors and Awards

1999, Creative Research Medal, University of Georgia
1990-1995, Research Career Development Award, National Institutes of Health

Previous Positions

2000-2006, Department Head, University of Georgia, College of Arts and Sciences, Microbiology
1991-1996, Associate Professor, University of Georgia, College of Arts and Sciences, Microbiology
1985-1990, Assistant Professor, University of Georgia, Microbiology
1983-1985, Postdoctoral, University of South Alabama
1981-1982, Graduate Research Assistant, University of Texas Health Science Center at San Antonio
1978-1980, Graduate Research Assistant, University of North Carolina

Patents

Method of Detecting Mycoplasma Infection in Poultry and Compositions Thereof, US patent pending

Funding Received

  • National Institutes of Health (NIH): Mycoplasma Pneumoniae Gliding Motility, $150,000 (year 04), 2002 to 2006.
  • National Institutes of Health (NIH): Protein Phosphorylation in Mycoplasma Pneumoniae, $61,315 (year 04), 1993 to 1997.
  • National Institutes of Health (NIH): A Genetic APproach to Mycoplasma Pneumoniae Virulence, $68,580 (year 05), 1990 to 1995.
  • National Institutes of Health (NIH): Genetic Analysis of Mycoplasma Pneumoniae Cell Adherence, $200,000 (year 21), 1986 to 2009.

Publications

  • Hasselbring BM, Jordan JL, Krause DC (Sep 2005) Mutant analysis reveals a specific requirement for protein P30 in Mycoplasma pneumoniae gliding motility., Journal of Bacteriology, 187 (18), 6281-9 Abstract
  • Waldo RH 3rd, Jordan JL, Krause DC (Jan 2005) Identification and complementation of a mutation associated with loss of Mycoplasma pneumoniae virulence-specific proteins B and C., Journal of Bacteriology, 187 (2), 747-51 Abstract
  • Balish, M.F., Krause, D.C. (2005) Mycoplasma attachment organelle and cell division In Blanchard, A., Browning, G. (eds), Mycoplasmas: Molecular biology, Pathogenicity, and strategies for control, Norfolk, UK, Horizon Scientific Press, 189-237 pages (bookchapter)
  • Balish MF, Santurri RT, Ricci AM, Lee KK, Krause DC, Localization of Mycoplasma pneumoniae cytadherence-associated protein HMW2 by fusion with green fluorescent protein: implications for attachment organelle structure, Molecular Microbiology, 47(1), 49-60, January 2003 Abstract
  • Willby MJ, Krause DC, Characterization of a Mycoplasma pneumoniae hmw3 mutant: implications for attachment organelle assembly, Journal of Bacteriology, 184(11), 3061-8, June 2002 Abstract
  • Balish, M.F., Krause, D.C. (2002) Cytoskeletal structure and function in Mycoplasma pneumoniae In Razin, S., Herrmann, R. (eds), Molecular Biology and Pathogenicity of Mycoplasmas, New York, Kluwer Academic / Plenum Publishers, 49-518 pages (bookchapter)
  • Jordan JL, Berry KM, Balish MF, Krause DC, Stability and subcellular localization of cytadherence-associated protein P65 in Mycoplasma pneumoniae, Journal of Bacteriology, 183(24), 7387-91, December 2001 Abstract
  • Balish MF, Hahn TW, Popham PL, Krause DC, Stability of Mycoplasma pneumoniae cytadherence-accessory protein HMW1 correlates with its association with the triton shell, Journal of Bacteriology, 183(12), 3680-8, June 2001 Abstract
  • Krause DC, Balish MF, Structure, function, and assembly of the terminal organelle of Mycoplasma pneumoniae, Fems Microbiology Letters, 198(1), 1-7, April 2001 Abstract
  • Greene, C.E., Krause, D.C. (2000) Bartonellosis In Brown, C., Bolin, C. (eds), Emerging Infectious Diseases of Animals, Washington, DC, American Society for Microbiology (bookchapter)
  • Waldo RH 3rd, Popham PL, Romero-Arroyo CE, Mothershed EA, Lee KK, Krause DC, Transcriptional analysis of the hmw gene cluster of Mycoplasma pneumoniae., Journal of Bacteriology, 181(16), 4978-85, August 1999 Abstract
  • Fisseha M, Gohlmann HW, Herrmann R, Krause DC, Identification and complementation of frameshift mutations associated with loss of cytadherence in Mycoplasma pneumoniae., Journal of Bacteriology, 181(14), 4404-10, July 1999 Abstract
  • Hahn TW, Mothershed EA, Waldo RH 3rd, Krause DC, Construction and analysis of a modified Tn4001 conferring chloramphenicol resistance in Mycoplasma pneumoniae, Plasmid, 41(2), 120-4, March 1999 Abstract
  • Romero-Arroyo CE, Jordan J, Peacock SJ, Willby MJ, Farmer MA, Krause DC, Mycoplasma pneumoniae protein P30 is required for cytadherence and associated with proper cell development, Journal of Bacteriology, 181(4), 1079-87, February 1999 Abstract
  • Mehock JR, Greene CE, Gherardini FC, Hahn TW, Krause DC, Bartonella henselae invasion of feline erythrocytes in vitro., Infection and Immunity, 66(7), 3462-6, 1998 Abstract
  • Hahn TW, Willby MJ, Krause DC, HMW1 is required for cytadhesin P1 trafficking to the attachment organelle in Mycoplasma pneumoniae, Journal of Bacteriology, 180(5), 1270-6, March 1998 Abstract
  • Krause DC, Mycoplasma pneumoniae cytadherence: organization and assembly of the attachment organelle, Trends in Microbiology, 6(1), 15-8, Jan 1998 Abstract
  • Krause DC, Mycoplasma pneumoniae cytadherence: organization and assembly of the attachment organelle., Trends in Microbiology, 6(1), 15-8, 1998 Abstract
  • Popham PL, Hahn TW, Krebes KA, Krause DC, Loss of HMW1 and HMW3 in noncytadhering mutants of Mycoplasma pneumoniae occurs post-translationally, Proceedings of the National Academy of Sciences (USA), 94(25), 13979-84, December 1997 Abstract
  • Krause DC, Proft T, Hedreyda CT, Hilbert H, Plagens H, Herrmann R, Transposon mutagenesis reinforces the correlation between Mycoplasma pneumoniae cytoskeletal protein HMW2 and cytadherence, Journal of Bacteriology, 179(8), 2668-77, April 1997 Abstract
  • Dirksen L B, Proft T, Hilbert H, Plagens H, Herrmann R, Krause D C, Sequence analysis and characterization of the hmw gene cluster of Mycoplasma pneumoniae., Gene, 171(1), 19-25, 24 May 1996 Abstract
  • Krause DC, Mycoplasma pneumoniae cytadherence: unravelling the tie that binds, Molecular Microbiology, 20(2), 247-53, April 1996 Abstract
  • Hedreyda CT, Krause DC, Identification of a possible cytadherence regulatory locus in Mycoplasma pneumoniae, Infection and Immunity, 63(9), 3479-83, September 1995 Abstract
  • Krebes KA, Dirksen LB, Krause DC, Phosphorylation of Mycoplasma pneumoniae cytadherence-accessory proteins in cell extracts, Journal of Bacteriology, 177(15), 4571-4, August 1995 Abstract
  • Krause, D.C., Stevens, M.K. (1995) Localization of antigens on mycoplasma cell surface and tip structures In Razin, S., Tully, J.G. (eds), Molecular and Diagnostic Procedures in Mycoplasmology, Orlando, FL, Academic Press, 89-98 pages (bookchapter)
  • Hedreyda C T, Lee K K, Krause D C, Transformation of Mycoplasma pneumoniae with Tn4001 by electroporation., Plasmid, 30(2), 170-5, September 1993 Abstract
  • Ogle K F, Lee K K, Krause D C, Nucleotide sequence analysis reveals novel features of the phase-variable cytadherence accessory protein HMW3 of Mycoplasma pneumoniae., Infection and Immunity, 60(4), 1633-41, April 1992 Abstract
  • Krause, D.C., Taylor-Robinson, D. (1992) Mycoplasma which infect humans, Mycoplasmas: Molecular Biology and Pathogenesis. (Maniloff, J., R. N. McElhaney, L. R. Finch, and J. B. Baseman, eds), Washington, DC, American Society for Microbiology, 417-444 pages (bookchapter)
  • Krause D C, Lee K K, Juxtaposition of the genes encoding Mycoplasma pneumoniae cytadherence-accessory proteins HMW1 and HMW3., Gene, 107(1), 83-9, 30 Oct 1991 Abstract
  • Stevens M K, Krause D C, Localization of the Mycoplasma pneumoniae cytadherence-accessory proteins HMW1 and HMW4 in the cytoskeletonlike Triton shell., Journal of Bacteriology, 173(3), 1041-50, February 1991 Abstract
  • Ogle K F, Lee K K, Krause D C, Cloning and analysis of the gene encoding the cytadherence phase-variable protein HMW3 from Mycoplasma pneumoniae., Gene, 97(1), 69-75, 2 Jan 1991 Abstract
  • Stevens M K, Krause D C, Disulfide-linked protein associated with Mycoplasma pneumoniae cytadherence phase variation., Infection and Immunity, 58(10), 3430-3, October 1990 Abstract
  • Krause D C, Mawn C B, Physical analysis and mapping of the Mycoplasma pneumoniae chromosome., Journal of Bacteriology, 172(9), 4790-7, September 1990 Abstract
  • Krause D C, Kleven S H, Lee K K, Cloning and expression in Escherichia coli of Mycoplasma gallisepticum antigens recognized by sera from infected chickens., Avian Diseases, 34(2), 466-72, April 1990 Abstract
  • Williamson LR, Plano GV, Winkler HH, Krause DC, Wood DO, Nucleotide sequence of the Rickettsia prowazekii ATP/ADP translocase-encoding gene, Gene, 80(2), 269-78, 1989 Abstract
  • Krause DC, Chen YY, Interaction of Mycoplasma pneumoniae with HeLa cells, Infection and Immunity, 56(8), 2054-9, August 1988 Abstract
  • Chen YY, Krause DC, Parasitism of hamster trachea epithelial cells by Mycoplasma pneumoniae, Infection and Immunity, 56(3), 570-6, March 1988 Abstract
  • Wood DO, Williamson LR, Winkler HH, Krause DC, Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene, Journal of Bacteriology, 169(8), 3564-72, August 1987 Abstract
  • Krause DC, Winkler HH, Wood DO, Cloning and expression of the Rickettsia prowazekii ADP/ATP translocator in Escherichia coli, Proceedings of the National Academy of Sciences (USA), 82(9), 3015-9, May 1985 Abstract
  • Krause DC, Winkler HH, Wood DO, Cosmid cloning of Rickettsia prowazekii antigens in Escherichia coli K-12, Infection and Immunity, 47(1), 157-65, January 1985 Abstract
  • Krause DC, Baseman JB, Inhibition of mycoplasma pneumoniae hemadsorption and adherence to respiratory epithelium by antibodies to a membrane protein, Infection and Immunity, 39(3), 1180-6, March 1983 Abstract
  • Krause DC, Leith DK, Baseman JB, Reacquisition of specific proteins confers virulence in Mycoplasma pneumoniae, Infection and Immunity, 39(2), 830-6, February 1983 Abstract
  • Leith DK, Hansen EJ, Wilson RM, Krause DC, Baseman JB, Hemadsorption and virulence are separable properties of Mycoplasma pneumoniae, Infection and Immunity, 39(2), 844-50, February 1983 Abstract
  • Baseman JB, Cole RM, Krause DC, Leith DK, Molecular basis for cytadsorption of Mycoplasma pneumoniae, Journal of Bacteriology, 151(3), 1514-22, September 1982 Abstract
  • Krause DC, Baseman JB, Mycoplasma pneumoniae proteins that selectively bind to host cells, Infection and Immunity, 37(1), 382-6, July 1982 Abstract
  • Krause DC, Leith DK, Wilson RM, Baseman JB, Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence, Infection and Immunity, 35(3), 809-17, March 1982 Abstract
  • Krause, D.C,, Jordan, J.L., Chang, H-Y., Park, H.K., Krunkosky, T.M., Mycoplasma pneumoniae attachment and colonization of the respiratory mucosa In Brogden, K.A., Minion, F.C., Stanton, T.B., Zhang, Q., Nolan, L., Wannemuehler, M.J. (eds), , Virulence Mechanisms of Bacterial Pathogens, 4th Edition, Washington, DC, American Society for Microbiology, In Press (bookchapter)
  • Hahn, T.-W., K.A. Krebes, and D.C. Krause.1996. Expression in Mycoplasma pneumoniae of the recombinant gene encoding the cytadherence-associated protein HMW1 and identification of HMW4 as a product. Molecular Microbiol.19:1085-1093
  • Norman, A.F., R. Regnery, P. Jameson, C. Greene, and D.C. Krause. 1995. Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene. J. Clin. Microbiol. 33:1797-1803
  • Hedreyda, C.T. and D.C. Krause. 1995. Identification of a possible cytadherence regulatory locus in Mycoplasma pneumoniae. Infect. Immun. 63:3479-3483
  • Dirksen, L.B., K.A. Krebes and D.C. Krause. 1994. Phosphorylation of Cytadherence-accessory proteins in Mycoplasma pneumoniae. J. Bactweriol. 176:7499-7505
  • Stevens, M.K. and D.C. Krause. 1992. Mycoplasma pneumonia cytadherence phase-variable protein HMW3 is a component of the attachment organelle. J. Bacteriol. 1174: 4265-4274

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Last Updated: 3/5/2007

COS Expertise ID #231765
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