Mary F. Lampe

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University of Washington
School of Medicine
Medicine
Allergy and Infectious Diseases
AdjunctAppointed: 2008
University of Washington
School of Medicine
Microbiology
AdjunctAppointed: 1998
University of Washington
School of Medicine
Laboratory Medicine
Medical Technology Program
DirectorAppointed: 1998
University of Washington
School of Medicine
Laboratory Medicine
Associate ProfessorAppointed: 1989

Mailing Address

Box 357110/NW120
1959 NE Pacific St.
Dept. of Laboratory Medicine
University of Washington
Seattle, Washington 98195-7110
United States

Contact Information

Phone: (206) 598-2135
Fax: (206) 598-6189
lampe@u.washington.edu

Qualifications

Ph.D., University of North Carolina at Chapel Hill, 1984.
M.S., University of Washington, Microbiology, 1976.
B.S., University of Washington, Medical Technology, 1968.

Expertise and Research Interests

Research in this laboratory focuses on the molecular analysis of Chlamydial pathogenesis and intervention in Chlamydia trachomatis infection. Specific research projects include:

In vitro sensitivity of C. trachomatis to topical microbicides
The goal of these studies is to identify microbicides that prevent infection by C. trachomatis when applied vaginally. My laboratory was one of the first to develop the Minimal Cidal Concentration (MCC) assay to test the action of topical microbicides against this important sexually transmitted pathogen. We are currently testing the peptide WLBU2 and the lipid 3-octyl glycerol from human milk alone and in combination and have found that they are very active at concentrations and for times that could be achieved vaginally. My laboratory is now serving as a national and international resource for topical microbicide testing against C. trachomatis.

Analysis of C. trachomatis autotransporter PmpD
This gene is a member of a family of autotransporter genes, a suprising finding in the small genome of this obligate intracellular bacterium. My laboratory cloned the pmpD gene, determined its nucleotide sequence, analyzed its time of expression, studied its localization in C. trachomatis infected cells, and examined its post-translational processing. Future projects include determining its function in the C. trachomatis developmental cycle and comparing it with other Pmps.

Other Expertise

Academic Experience:
Manuscript reviewer:
Antimicrobial Agents and Chemotherapy
Clinical Infectious Diseases
Genitourinary Medicine
Infection and Immunity
Journal of Clinical Microbiology
Journal of Infectious Diseases
Microbiological Reviews
Obstetrics and Gynecology

Keywords

COS Keywords:

Bacteria, Bacteriology, Biochemistry, DNA, Gene Cloning, Inflammation, Microbial Pathogenesis, Sexually Transmitted Diseases, Tumor Immunology.

Additional Terms:

Anti-Chlamydial Lipid, Anti-Chlamydial Peptide, Autotransporter Proteins, Bacteria, Bacterial Disease, Chlamydia, Chlamydia trachomatis, Infectious Disease, Microbial Pathogenesis, Pmps, Sexually Transmitted Infection, Topical Microbicide, Virulence Factors.

Languages

(Reading, Writing, Speaking)

French: (Basic, Basic, Functional)
Amharic: (None, None, Basic)

Memberships

Academy of Clinical Laboratory Physicians and Scientists
American Society for Clinical Laboratory Science
American Society for Microbiology
Northwest Branch, American Society for Microbiology

Honors and Awards

1994-1995, Royalty Research Fund Award, University of Washington
1992-1994, New Investigator Award, University of Washington STD Cooperative Research Center
1984-1984, Mary Poston Award, North Carolina Branch, American Society for Microbiology
1984-1985, Bourse Chateaubriand, French Government
1984-1985, Research Award, Pierre Philippe Foundation

Previous Positions

1988-1989, Postdoctoral Fellow, University of Washington
1985-1987, Postdoctoral Fellow, Harvard University
1984-1985, Institut Pasteur (France), Postdoctoral Fellow
1982, University of North Carolina, Chapel Hill, Carolina Workshop on DNA Sequencing and General DNA Technology, Molecular Biology and Biotechnology, Teaching Assistant

Funding Received

  • National Institutes of Health (NIH): 2007-2012 USPHS/NIH MIPII, R21, 2007 to 2009.
  • National Institutes of Health (NIH): USPHS/NIH, UO1 AI31448, Univ. of Wash. STD Cooperative Research Center Project, 1999 to 2004.
  • National Institutes of Health (NIH): USPHS/NIH, UO1 AI31448, Univ. of Wash. STD Cooperative Research Center Laboratory Core, 1999 to 2004.
  • National Institutes of Health (NIH): USPHS/NIH, PO1 AI39061-05, Top. Microbicide Prog. Proj., Chlamydia trachomatis laboratory core, 1999 to 2005.
  • National Institutes of Health (NIH): USPHS/NIH, PO1 AI37830, Top. Microbicide Prog. Proj., Chlamydia trachomatis laboratory core, 1995 to 1999.
  • National Institutes of Health (NIH): USPHS/NIH, UO1 AI31448, Univ. of Wash. STD Cooperative Research Center Project, 1995 to 1999.
  • National Institutes of Health (NIH): USPHS/NIH, UO1 AI31448, Univ. of Wash. STD Cooperative Research Center, Chlamydial/Gonococcal Laboratory Core, 1995 to 1999.
  • University of Washington: Royalty Research Fund Grant, 1994 to 1995.
  • National Institutes of Health (NIH): New Investigator Award, University of Washington STD Cooperative Research Center, 1992 to 1995.

Publications

  • Lampe M F, Ballweber L M, Stamm W E, Susceptibility of Chlamydia trachomatis to chlorhexidine gluconate gel., Antimicrobial Agents and Chemotherapy, 42(7), 1726-30, July 1998 Abstract
  • Lampe M F, Ballweber L M, Isaacs C E, Patton D L, Stamm W E, Killing of Chlamydia trachomatis by novel antimicrobial lipids adapted from compounds in human breast milk., Antimicrobial Agents and Chemotherapy, 42(5), 1239-44, May 1998 Abstract
  • Lampe, MF, Ballweber, LM, Stamm, WE, Susceptibility of Chlamydia trachomatis to Chlorhexidine Gluconate Gel, Antimicrobial Agents and Chemotherapy, 42, 1726-30, 1998
  • Lampe, MF, Ballweber, LM, Isaacs, CE, Klebanoff, SJ, Stamm, WE, Susceptibility of Chlamydia trachomatis to Novel Topical Microbicides, Chlamydial Infections, Proceedings of the Ninth International Symposium on Human Chlamydial Infections, 1998
  • Lampe, MF, Ballweber, LM, Stamm, WE, Cloning and Sequence Analysis of a Putative Cytolysin from Chlamydia trachomatis, Chlamydial Infections, Proceedings of the Ninth International Symposium on Human Chlamydial Infections, 1998
  • Lampe, MF, Ballweber, LM, Isaacs, CE, Patton, DL, Stamm WE, Killing of Chlamydia trachomatis by Novel Antimicrobial Lipids Adapted from Compounds in Human Breast Milk, Antimicrobial Agents and Chemotherapy, 42, 1239-44, 1998
  • Lampe, MF, Wilson, CB, Bevan, MJ, Starnbach, MN, The Role of Interferon-g in the Resolution of Chlamydia trachomatis Infection by Cytotoxic T-lymphocytes, Chlamydial Infections, Proceedings of the Ninth Internationa, 1998
  • Lampe M F, Wong K G, Kuehl L M, Stamm W E, Chlamydia trachomatis major outer membrane protein variants escape neutralization by both monoclonal antibodies and human immune sera, Infection and Immunity, 65(1), 317-9, January 1997 Abstract
  • Lampe, M.F., Ballweber, L.M., Borders, M.A., Isaacs, C.E., Stamm, W.E, A New Approach to Control Sexually Transmitted Diseases: Effect of Novel Topical Microbicides on Chlamydia trachomatis, Proceedings, Third Meeting of the European Society for Chlamydia Research, 1996
  • Starnbach M N, Bevan M J, Lampe M F, Murine cytotoxic T lymphocytes induced following Chlamydia trachomatis intraperitoneal or genital tract infection respond to cells infected with multiple serovars, Infection and Immunity, 63(9), 3527-30, September 1995 Abstract
  • Lampe M F, Wong K G, Stamm W E, Sequence conservation in the major outer membrane protein gene among Chlamydia trachomatis strains isolated from the upper and lower urogenital tract, Journal of Infectious Diseases, 172(2), 589-92, August 1995 Abstract
  • Bauwens J E, Lampe M F, Suchland R J, Wong K, Stamm W E, Infection with Chlamydia trachomatis lymphogranuloma venereum serovar L1 in homosexual men with proctitis: molecular analysis of an unusual case cluster, Clinical Infectious Diseases, 20(3), 576-81, March 1995 Abstract
  • Starnbach M N, Bevan M J, Lampe M F, Protective cytotoxic T lymphocytes are induced during murine infection with Chlamydia trachomatis., Journal of Immunology, 153(11), 5183-9, 1 Dec 1994 Abstract
  • Lampe M F, Stamm W E, Purification of Chlamydia trachomatis strains in mixed infection by monoclonal antibody neutralization, Journal of Clinical Microbiology, 32(2), 533-5, February 1994 Abstract
  • Workowski K A, Lampe M F, Wong K G, Watts M B, Stamm W E, Long-term eradication of Chlamydia trachomatis genital infection after antimicrobial therapy. Evidence against persistent infection [see comments] [published erratum appears in JAMA 1994 Feb 2;271(5):, Jama, 270(17), 2071-5, 3 Nov 1993 Abstract
  • Patton D L, Wolner-Hanssen P, Zeng W, Lampe M, Wong K, Stamm W E, Holmes K K, The role of spermatozoa in the pathogenesis of Chlamydia trachomatis salpingitis in a primate model, Sexually Transmitted Diseases, 20(4), 214-9, Summer 1993 Abstract
  • Lampe M F, Suchland R J, Stamm W E, Nucleotide sequence of the variable domains within the major outer membrane protein gene from serovariants of Chlamydia trachomatis, Infection and Immunity, 61(1), 213-9, January 1993 Abstract
  • Starnbach, M.N., M.J. Bevan, M.F. Lampe, 'Protective Cytotoxic T-Lymphocytes are Induced During Murine Infection with 'Chlamydia Trachomatis,' J. Immunol., 153:5183-5189, 1994
  • Lampe, M.F., L.M. Kuehl, K.G. Wong, W.E. Stamm, ''Chlamydia Trachomatis' Major Outer Membrane Protein Variants Escape Neutralization by Polyclonal Human Immune Sera,' Chlamydial Infections, Proceedings of the Eighth International Symposium on Human Chlamydial Infections, J. Schachter, J. Chernesky, T. Grayston, R.B. Jones, J. Orifila, G. Ridgway, R.S. Stephens, W.E. Stamm (eds.), New York: Cambridge University Press, 1994
  • Lampe, MF, Wilson, CB, Bevan, MJ, Starnbach, MN, The Role of Interferon-g in the Resolution of Chlamydia trachomatis Infection by Cytotoxic T-lymphocytes, Journal of Immunology, in press

Profile Details

Last Updated: 6/25/2009

COS Expertise ID #308519
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