Dr. Linzhao Cheng

(Reading, Writing, Speaking)

Stem Cell Research Foundation (USA): Gene traps in human embryonic stem cells using improved lentiviral vectors, $50,000 per year, 2005 to 2007.
National Institutes of Health (NIH): Directing human ES and EBD cells to blood/immune cells, ~$405,000 per year, 2003 to 2009.
National Institutes of Health (NIH): In vivo Molecular and Cellular Imaging Center for Cancer Research, 2003 to 2008.
National Institutes of Health (NIH): Hematopoietic Stem Cells for Transplantation, 2002 to 2007.

• Mali P, Ye Z, Hommond HH, Yu X, Lin J, Chen G, Zou J, Cheng L (May 2008) Improved Efficiency and Pace of Generating Induced Pluripotent Stem Cells from Human Adult and Fetal Fibroblasts., Stem cells (Dayton, Ohio)
• Yu X, Zou J, Ye Z, Hammond H, Chen G, Tokunaga A, Mali P, Li YM, Civin C, Gaiano N, Cheng L (May 2008) Notch signaling activation in human embryonic stem cells is required for embryonic, but not trophoblastic, lineage commitment., Cell stem cell, 2 (5), 461-71
• Chen G, Ye Z, Yu X, Zou J, Mali P, Brodsky RA, Cheng L (Apr 2008) Trophoblast differentiation defect in human embryonic stem cells lacking PIG-A and GPI-anchored cell-surface proteins., Cell stem cell, 2 (4), 345-55
• Cheng L (Feb 2008) More new lines of human parthenogenetic embryonic stem cells., Cell research, 18 (2), 215-7
• Cheng L, Xiao L, Zeng F, Zhang YA (Jan 2008) Stem cells shine in Shanghai., Cell stem cell, 2 (1), 34-7
• Ye Z, Yu X, Cheng L (2008) Lentiviral gene transduction of mouse and human stem cells., Methods in molecular biology (Clifton, N.J.), 430, 243-53
• Gao P (2007) HIF-dependent Anti-tumorigenic Effect of Anti-oxidants In Vivo, Cancer Cell, 12 (3), 230-238
• Li L, Piloto O, Kim KT, Ye Z, Nguyen HB, Yu X, Levis M, Cheng L, Small D (Apr 2007) FLT3/ITD expression increases expansion, survival and entry into cell cycle of human haematopoietic stem/progenitor cells., British journal of haematology, 137 (1), 64-75
• Zhou BY, Ye Z, Chen G, Gao ZP, Zhang YA, Cheng L (Mar 2007) Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer., Stem cells (Dayton, Ohio), 25 (3), 779-89
• Ohm JE, McGarvey KM, Yu X, Cheng L, Schuebel KE, Cope L, Mohammad HP, Chen W, Daniel VC, Yu W, Berman DM, Jenuwein T, Pruitt K, Sharkis SJ, Watkins DN, Herman JG, Baylin SB (Feb 2007) A stem cell-like chromatin pattern may predispose tumor suppressor genes to DNA hypermethylation and heritable silencing., Nature genetics, 39 (2), 237-42
• Cai L, Ye Z, Zhou BY, Mali P, Zhou C, Cheng L (Jan 2007) Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions., Cell research, 17 (1), 62-72
• Mali P, Cheng L (2006) Re-programming somatic cells without fusion or ethical confusion (an invited review), Regenerative Medicine, 1 (6), 837
• Dravid G, Ye Z, Hammond H, Chen G, Pyle A, Donovan P, Yu X, Cheng L (2006) Defining the role of Wnt/beta-catenin signaling in the survival, proliferation and self-renewal of human embryonic stem cells, Stem Cells, 23 (7), 1489
• Cheng L (2006) Ethics: China already has clear stem-cell guidelines, Nature, 440, 992
• Dravid G, Hammond H, Cheng L (2006) Culture of human embryonic stem cells on human and mouse feeder cells., Methods in molecular biology (Clifton, N.J.), 331, 91-104
• Cheng L (2005) Experimental protocols Sectiond offers expert insights for stem cell research (editorial), Stem Cells, 23, 297-298
• Du X, Et Al., Radiofrequency-enhanced Vascular Gene Transduction and Expression For, Radiology, 236(3), 939-44, Sep 2005
• Zhan X, Dravid G, Ye Z, Hammond H, Shamblott M, Gearhart J, Cheng L, Functional Antigen-presenting Leucocytes Derived From Human Embryonic Stem Cells in Vitro, Lancet, 364(9429), 163-71, Jul 2004
• Pan F, Ye Z, Cheng L, Liu JO ( Apr 2004) Myocyte Enhancer Factor 2 Mediates Calcium-dependent Transcription of The Interleukin-2 Gene in T Lymphocytes: a Calcium Signaling Module That Is Distinct From But Collaborates With the Nuclear Factor of Activated T Cells (NFAT), The Journal of Biological Chemistry, 279 (15), 14477-80
• Chen HH, Zhan X, Kumar A, Du X, Hammond H, Cheng L, Yang X ( Nov-Dec 2004) Detection of Dual-gene Expression in Arteries Using An Optical Imaging Method, Journal of Biomedical Optics, 9 (6), 1223-9
• Cui Y, Kelleher E, Straley E, Fuchs E, Gorski K, Levitsky H, Borrello I, Civin CI, Schoenberger SP, Cheng L, Pardoll DM, Whartenby KA, Immunotherapy of established tumors using bone marrow transplantation with antigen gene--modified hematopoietic stem cells, Nature Medicine, 9(7), 952-8, July 2003
• Zhou X, Cui Y, Huang X, Yu Z, Thomas AM, Ye Z, Pardoll DM, Jaffee EM, Cheng L, Lentivirus-mediated gene transfer and expression in established human tumor antigen-specific cytotoxic T cells and primary unstimulated T cells, Human Gene Therapy, 14(11), 1089-105, July 2003
• Yu X, Zhan X, D&#146, Costa J, Tanavde VM, Ye Z, Peng T, Malehorn MT, Yang X, Civin CI and Cheng L, Lentiviral Vectors with Two Independent Internal Promoters Transfer High-Level Expression of Multiple Transgenes to Human Hematopoietic Stem-Progenitor Cells, Molecular Therapy, 7(6), 827, June 2003
• Angelopoulou M, Novelli E, Grove JE, Henry M. Rinder M, Civin C, Cheng L, Krause DS, Cotransplantation Of Human Mesenchymal Stem Cells Enhances Human Myelopoiesis And Megakaryocytopoiesis In NOD/SCID Mice, Experimental Hematology, 31(5), 413, May 2003
• Cheng L, Hammond H, Ye Z, Zhan X, Dravid G, Human Adult Marrow Cells Support Prolonged Expansion of Human Embryonic Stem Cells in Culture, Stem Cells (dayton, Ohio), 21(2), 131-42, 2003
• Cui Y, Golob J, Kelleher E, Ye Z, Pardoll D, Cheng L, Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells, Blood, 99(2), 399-408, January 2002
• Yang X, Atalar E, Li D, Serfaty JM, Wang D, Kumar A, Cheng L, Magnetic resonance imaging permits in vivo monitoring of catheter-based vascular gene delivery, Circulation, 104(14), 1588-90, 2001
• Yang X, Liu H, Li D, Zhou X, Jung WC, Deans AE, Cui Y, Cheng L, Digital optical imaging of green fluorescent proteins for tracking vascular gene expression: feasibility study in rabbit and human cell models, Radiology, 219(1), 171-5, April 2001
• Shamblott MJ, Axelman J, Littlefield JW, Blumenthal PD, Huggins GR, Cui Y, Cheng L, Gearhart JD (2001) Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro, Proceedings of the National Academy of Sciences (USA), 113-8 pages
• Gao Z, Golob J, Tanavde VM, Civin CI, Hawley RG, Cheng L, High levels of transgene expression following transduction of long-term NOD/SCID-repopulating human cells with a modified lentiviral vector, Stem Cells, 19(3), 247-59, 2001
• Cheng L, Qasba P, Vanguri P, Thiede MA (July 2000) Human mesenchymal stem cells support megakaryocyte and pro-platelet formation from CD34(+) hematopoietic progenitor cells, Journal of Cellular Physiology, 184 (1), 58-69
• Liu X, Rapp N, Deans R, Cheng L, Molecular cloning and chromosomal mapping of a candidate cytokine gene selectively expressed in human CD34 cells, Genomics, 65(3), 283-92, 2000
• Novelli EM, Cheng L, Yang Y, Leung W, Ramirez M, Tanavde V, Enger C, Civin CI, Ex vivo culture of cord blood CD34 cells expands progenitor cell numbers, preserves engraftment capacity in nonobese diabetic/severe combined immunodeficient mice, and enhances retroviral transductio, Human Gene Therapy, 10(18), 2927-40, 1999
• Mbalaviele G, Jaiswal N, Meng A, Cheng L, Van Den Bos C, Thiede M, Human mesenchymal stem cells promote human osteoclast differentiation from CD34 bone marrow hematopoietic progenitors, Endocrinology, 140(8), 3736-43, August 1999
• Cheng L, Du C, Lavau C, Chen S, Tong J, Chen BP, Scollay R, Hawley RG, Hill B, Sustained gene expression in retrovirally transduced, engrafting human hematopoietic stem cells and their lympho-myeloid progeny, Blood, 92(1), 83-92, 1998
• Cheng L, Du C, Murray D, Tong X, Zhang YA, Chen BP, Hawley RG, A GFP reporter system to assess gene transfer and expression in human hematopoietic progenitor cells, Gene Therapy, 4(10), 1013-22, October 1997
• Cheng L, Fu J, Tsukamoto A, Hawley RG, Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells, Nature Biotechnology, 14(5), 606-9, May 1996
• Cheng L, Gearing DP, White LS, Compton DL, Schooley K, Donovan PJ, Role of leukemia inhibitory factor and its receptor in mouse primordial germ cell growth, Development, 120(11), 3145-53, November 1994
• Resnick JL, Bixler LS, Cheng L, Donovan PJ, Long-term proliferation of mouse primordial germ cells in culture, Nature, 359(6395), 550-1, 1992
• Cheng LZ, Workman JL, Kingston RE, Kelly TJ (1992) Regulation of DNA replication in vitro by the transcriptional activation domain of GAL4-VP16, Proceedings of the National Academy of Sciences (USA), 589-93 pages
• Cheng L, Kelly TJ, Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro, Cell, 59(3), 541-51, 1989
• Wang K, Xue T, Tsang SY, Van Huizen R, Wong CW, Lai KW, Ye Z, Cheng L, Au KW, Zhang J, Li GR, Lau CP, Tse HF, Li RA, Electrophysiological properties of pluripotent human and mouse embryonic stem cells., Stem cells (Dayton, Ohio), 23 (10), 1526-34
• Dravid G, Ye Z, Hammond H, Chen G, Pyle A, Donovan P, Yu X, Cheng L, Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells., Stem cells (Dayton, Ohio), 23 (10), 1489-501