Prof. David A. Lightfoot

(Reading, Writing, Speaking)

United States Department of Agriculture (USDA): Automated Methods for Selection of Resistance, $315,244, Sep 1, 1998 to Aug 31, 2001.
United Soybean Board: Biotechnology Approaches to SCN Resistance, $900,000, Oct 1, 1998 to 2007.
National Science Foundation (NSF): Integrated Physical Map of the Soybean Genome, $1,326,044, Oct 1, 1998 to June 30, 2004.
Illinois Soybean Board: Biotechnology Research, SDS and SCN Panels, $225,000, Mar 1, 2001 to 2006.
Council For Agricultural Reseach (CFAR): GMO Safety, $315,456, Jul 1, 2000 to Jun 30, 2004.
United Soybaean Board/North Central Soybean Bord: Application of Biotechnology to the Control of Sudden Death Syndrome, $1,350,000, Apr 1, 2002 to 2007.
SIUC OVCR Office: Interdisciplinary Biofuels Research: Biodiesel with High Energy Content, $30,000, 2007 to 2008.
IL CFAR: Aflatoxin Reduction in GDH Corn, $30,000, 2007 to 2008.
National Science Foundation (NSF): Multi-User Equipment for Proteomics, $250,000, 2004 to 2005.

• Lightfoot DA (Aug 2008) Blue revolution brings risks and rewards., Science (New York, N.Y.), 321 (5890), 771-2
• Kazi S, Shultz J, Afzal J, Johnson J, Njiti VN, Lightfoot DA (May 2008) Separate loci underlie resistance to root infection and leaf scorch during soybean sudden death syndrome., TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 116 (7), 967-77
• Afzal AJ, Wood AJ, Lightfoot DA (May 2008) Plant receptor-like serine threonine kinases: roles in signaling and plant defense., Molecular plant-microbe interactions : MPMI, 21 (5), 507-17
• Lightfoot DA (2008) Soybean Genomics: Developments through the Use of Cultivar "Forrest"., International journal of plant genomics, 2008, 793158
• Saini N, Shultz J, Lightfoot DA (2008) Re-annotation of the physical map of Glycine max for polyploid-like regions by BAC end sequence driven whole genome shotgun read assembly., BMC genomics, 9, 323
• Yuan J, Zhu M, Lightfoot DA, Iqbal MJ, Yang JY, Meksem K (2008) In silico comparison of transcript abundances during Arabidopsis thaliana and Glycine max resistance to Fusarium virguliforme., BMC genomics, 9 Suppl 2, S6
• Coyne CJ, McClendon MT, Walling JG, Timmerman-Vaughan GM, Murray S, Meksem K, Lightfoot DA, Shultz JL, Keller KE, Martin RR, Inglis DA, Rajesh PN, McPhee KE, Weeden NF, Grusak MA, Li CM, Storlie EW (Sep 2007) Construction and characterization of two bacterial artificial chromosome libraries of pea (Pisum sativum L.) for the isolation of economically important genes., Genome / National Research Council Canada = Génome / Conseil national de recherches Canada, 50 (9), 871-5
• Shultz JL,, Ali S,, Ballard L,, Lightfoot DA. (2007) Development of a physical map of the soybean pathogen Fusarium virguliforme based on synteny with Fusarium graminearum genomic DNA., BMC Genomics., 8, 262-270
• Afzal AJ, Lightfoot DA (Jun 2007) Soybean disease resistance protein RHG1-LRR domain expressed, purified and refolded from Escherichia coli inclusion bodies: preparation for a functional analysis., Protein expression and purification, 53 (2), 346-55
• Shultz JL, Kazi S, Bashir R, Afzal JA, Lightfoot DA (Apr 2007) The development of BAC-end sequence-based microsatellite markers and placement in the physical and genetic maps of soybean., TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 114 (6), 1081-90
• Lightfoot DA, Bernhardt, K.,, Mungur R.,, Nolte S.,, Ameziane R.,, Colter A,, Jones K,, MJ Iqbal,, Varsa EC,, Young BG. (2007) Lightfoot, D.A. Improved drought tolerance of transgenic Zea mays plants that express the glutamate dehydrogenase gene (gdhA) of E. coli., Euphytica, 156, 106-115.
• Shultz JL,, Ray JD, Lightfoot D.A. (2007) A sequence based synteny map between soybean and Arabidopsis thaliana., BMC Genomics, 8, 8-16
• Shultz J,, Kazi S.,, Bashir, R.,, Afzal J,, Lightfoot D.A. (2007) The development of BAC-end sequence-based microsatellite markers and placement in the physical and genetic maps of soybean, Theor Appl Genet, 114, 1081-1090
• Afzal AJ, Lightfoot DA (2007) Inclusion bodies contain RHG1 folding intermediates: A novel refolding protocol for protein purification., Protein Expression and Purification, 53, 346-355.
• Shultz JL, Ray JD, Lightfoot DA (2007) A sequence based synteny map between soybean and Arabidopsis thaliana., BMC genomics, 8, 8
• Shultz JL, Ali S, Ballard L, Lightfoot DA (2007) Development of a physical map of the soybean pathogen Fusarium virguliforme based on synteny with Fusarium graminearum genomic DNA., BMC genomics, 8, 262
• Shopinski K,, Iqbal J,, Afzal J,, Shultz J,, Jayaraman D,, Lightfoot DA. (2006) Development of a pooled probe method for locating small gene families in a physical map of soybean using stress related paralogues and a BAC minimum tile path., Plant Methods, 2, 20-28
• Ruben E, J. Aziz, Afzal J., V. N. Njiti, K., Triwitayakorn, M.J. Iqbal, S. Yaegashi, P. Arelli,, C. Town, K. Meksem and D. A. Lightfoot (15 Oct 2006) Genomic analysis of the `Peking' rhg1 locus: candidate genes that underlie soybean resistance to the cyst nematode., Molecular Genetics and Genomics, 276 (4), 320-330
• Kassem A, Shultz J., Njiti V., Meksem K.,, Wood AJ and D.A. Lightfoot (Oct 2006) An updated Essex and Forrest linkage map and first composite interval map of QTL underlying six soybean traits., Theor. Appl. Genet., 113 (6), 1015-1026
• Meyer, R, Z. Yuan, J. Afzal, J. Iqbal, David A. Lightfoot. (Aug 2006) Identification of Gsr1: A locus inferred to regulate gene expression in response to exogenous glutamine., Euphytica, 150, 291-302
• Shultz JL, Charles R Yesudas, Satsuki Yaegashi,, Jawaad Afzal, Samreen Kazi, David A Lightfoot (Jul 2006) Three minimum tile paths from bacterial artificial chromosome libraries of the soybean (Glycine max cv. 'Forrest'): Tools for structural and functional genomics., Plant Methods, 2, 9-18
• Mungur R, Wood AJ, Lightfoot DA (2006) Water potential during water deficit in transgenic plants: Nicotiana tabacum altered by the Escherichia coli glutamate dehydrogenase gene., Plant Growth Regulation, 50, 231-238.
• Njiti VN and Lightfoot DA. (Apr 2006) Genetic Analysis Infers Dt loci Underlie Resistance to SDS caused by Fusarium virguliforme in Indeterminate Soybeans, Can J Plant Science, 41, 83-89
• Shultz, J.L., Jayaraman, D., Shopinski, K.L., (1 Jan 2006) The Soybean Genome Database (SoyGD): A Browser For Display Of Duplicated, Polyploid, Regions and Sequence Tagged Sites On The Integrated Physical and Genetic Maps Of Glycine max., Nucleic Acid Research, 34, D758-765.
• Lightfoot DA, Njiti VN, Gibson PT,, Kassem MA, Iqbal JM, and Meksem K. (Aug 2005) Registration of Essex x Forrest recombinant inbred line (RIL) mapping population., Crop Science, 45 (4), 1678-1681
• Mungur R., Ameziane R., Glass AD, Goodenow DB and Lightfoot DA (2005) Metabolic Profiles of Transgenic Plants Expressing the Escherichia Coli Glutamate Dehydrogenase Gene, J. Biomed. Biotech, 2005 (2), 198-214
• Iqbal MJ, Yaegashi S, Ahsan R, Shopinski KL, Lightfoot DA (May 2005) Root response to Fusarium solani f. sp . glycines: temporal accumulation, Tag. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik., 110 (8), 1429-38
• Meksem K, Shultz J, Tebbji F, Jamai A, Henrich J, Kranz H, Arenz M, Schlueter T, Ishihara H, Jyothi LN, Zhang HB, Lightfoot DA (Apr 2005) A bacterial artificial chromosome based physical map of the Ustilago maydis genome., Genome / National Research Council Canada = Genome / Conseil National De Recherches Canada., 48 (2), 207-16
• Triwitayakorn K, Njiti VN, Iqbal MJ, Yaegashi S, Town C, Lightfoot DA (Feb 2005) Genomic analysis of a region encompassing QRfs1 and QRfs2: genes that underlie soybean resistance to sudden death syndrome., Genome / National Research Council Canada = Genome / Conseil National De Recherches Canada., 48 (1), 125-38
• Nolte SA, Young BG, Mungur R, and Lightfoot DA (Nov 2004) The Glutamate Dehydrogenase Gene gdhA Increased the Resistance of Tobacco to Glufosinate, Weed Science, 44, 334-339
• Wu C, Sun S, Nimmakayala P, Santos FA, Meksem K, Springman R, Ding K, Lightfoot DA, Zhang HB (Feb 2004) Construction and Characterization of a Soybean Bacterial Artificial Chromosome Library and Use of Multiple Complementary Libraries for Genome Physical Mapping, Theor Appl Genet., 109, 1041-1050
• Kassem A, Kang CH. Njiti V., Iqbal MJ., (May 2004) Loci Underlying Resistance to Manganese Toxicity Mapped in a Soybean Recombinant Inbred Line Population, Plant and Soil, 260, 183-196
• Iqbal M.J. and D.A. Lightfoot. (2004) Application of DNA Markers: Soybean Improvement. In Lörz, Horst; Wenzel, Gerhard (eds), Molecular Marker Systems in Plant Breeding and Crop Improvement, New York, Springer, 475 pages (bookchapter)
• Kassem A, Meksem K,. Njiti V., Iqbal MJ (Apr 2004) Definition of Soybean Genomic Regions That Control Seed Phytoestrogen Amounts, J Biotech Biomed, 2, 52-60
• Wu C, Sun S, Nimmakayala P, Santos FA, Springman R, Ding K, Meksem K, Lightfoot DA and Zhang HB (January 2004) A BAC and BIBAC-based Physical Map of the Soybean Genome, Genome Research, 14 ( Jan), 319-326
• Shultz J, Meksem K, Lightfoot DA. (Summer 2003) Evaluating Physical Maps By Clone Location Comparisons, Genome Letters, 2, 109-118
• Lightfoot DA, Meksem K., Zhang H-B, An integrated physical and genetic map for the soybean genome. In "Summaries of legume genomics projects from around the globe. Community resources for crops and models." Eds K Van den Bosch and G Sta, Plant Physiology, 131, 840-865, 2003
• Iqbal MJ, Yaegashi S, Njiti VN, Ahsan R, Cryder KL, Lightfoot DA (2003) A pyramid of loci for partial resistance to Fusarium solani f.sp. glycines maintains myo-inositol-1-phoshate synthase expression in soybean roots, Theor. Appl.. Genet, 1115-1123
• Cho Y, Njiti VN, Chen X, Lightfoot DA and Wood AJ (2003) Trigonelline concentration in field grown soybean in response to irrigation, Biologia Plantarum (46), 405-410
• Njiti, V., Myers O. Schroeder D. and D.A. Lightfoot (2003) Glyphosate on Roundup Ready soybean: Effects on root infection by Fusarium solani f. sp. glycines and Sudden Death Syndrome, Agronomy Journal, 95, 1140-1145
• Ashfield T, Bocian A, Held D, Henk A, Marek L, Danesh D, Penuela S, Meksem K, Lightfoot DA, Young N, Shoemaker R, Innes R (2003) Genetic and physical mapping of the soybean Rpg1-b disease resistance gene reveals a complex locus containing several tightly linked families of NBS/LRR genes, Genetics, 16, 817-826
• Shultz J, Meksem K, Lightfoot DA. (2003) Evaluating physical maps by clone location comparisons, Genome Letters, 2, 109-118
• Iqbal MJ, Yaegashi S, Ahsan R, Lightfoot DA, Banz WJ (December 2002) Differentially abundant mRNAs in rat liver in response to diets containing, Physiological Genomics (online), 11 (3), 219-26
• Iqbal MJ, Yaegashi S, Njiti VN, Ahsan R, Cryder KL, Lightfoot DA (November 2002) Resistance locus pyramids alter transcript abundance in soybean roots, Molecular Genetics and Genomics : Mgg, 268 (3), 407-17
• Yuan, Z., Njiti V., Meksem K., Iqbal MJ., Triwitakorn K., Kassem MA, Davis GT, Schmidt ME and D.A. Lightfoot (2002) Identification of yield loci in soybean populations that segregate for disease resistance, Crop Science, 42, 271-277
• Njiti V.N, Meksem K, Iqbal MJ, J. E. Johnson, K. F. Zobrist, V.Y. Kilo, D. A. Lightfoot. (2002) Common Loci Underlie Field Resistance to Soybean Sudden Death Syndrome in Forrest, Pyramid, Essex, and Douglas, Theoretical and Applied Genetics, 104, 294- 300
• Meksem K, Ruben E, Hyten D, Triwitayakorn K, Lightfoot DA (April 2001) Conversion of AFLP bands into high-throughput DNA markers, Molecular And General Genomics, 265 (2), 207-14
• Meksem K., Ruben E, Hyten D Schmidt M and D.A. Lightfoot. (01 Mar 2001) High-throughput detection of polymorphism physically linked soybean cyst nematode resistance gene Rhg4 using Taqman Probes, Molecular Breeding, 7 (3), 63-71
• Njiti, V., J.E. Johnson, G.A. Torto, L. Gray, Y. Luo, P.T. Gibson and D.A. Lightfoot (2001) Inoculum rate influences selection for field resistance to Sudden Death Syndrome in the greenhouse, Crop Science, 41, 1726-1723
• Iqbal J., Njiti, V., Meksem K. Kassem M. and D.A. Lightfoot. (01 Jan 2001) Microsatellite markers identify three additional quantitative trait loci for resistance to Soybean Sudden Death Syndrome (SDS) in Forrest and Essex RILs, Theoretical and Applied Genetics, 102, 187-192
• Men A, Meksem K., Kassem MA, Lothar, D., Stiller J, Lightfoot DA, Gresshoff P.M. (01 Jan 2001) A bacterial Artificail Chromosome Library of Lotus japonicus constructed in an Agrobacterium tumefaciens- transformable vector, Molecular Plant-Microbe Interactions, 14 (1), 422-425
• Zobrist K., Meksem K., Zhang H-B., and D.A. Lightfoot., A physical map for the soybean genome, Soybean Genetics Newsletter, 27, 5-10, 2000
• Ameziane R. Bates, R. Long L.M., Vidal, M.E., Lightfoot, D.A. (2000) Expression of the Escherichia coli glutamate dehydrogenase gene in tobacco affects plant growth and development, Plant and Soil, 221, 47-57
• Meksem K. Zhang H.B. and D.A. Lightfoot. (2000) Two transformation ready large insert clone libraries for soybean: Physical mapping of resistance to Soybean Cyst Nematode and Sudden Death Syndrome, Theoretical and Applied Genetics, 101, 747-755
• Njiti, V., K. Meksem, D.A. Lightfoot W.J. Banz and T.A. Winters. (2000) Molecular markers of phytoestrogen content in soybeans, Journal of Biomedicine and Biotechnology, 2, 1-8
• Cho Y, Lightfoot DA, Wood AJ (1999) . Survey of Trigonelline Concentrations in Salt-Stressed Leaves of Cultivated Glycine max, Phytochemistry, 52, 1235-1238
• Meksem K., Doubler T.W., Chang S.J.C., Chancharoenchai K., R. Suttner, P. Cregan, P. Rao Arelli, P.T. Gibson and D.A. Lightfoot. (1999) Clustering among genes underlying QTL for field resistance to Sudden Death Syndrome and cyst nematode race 3, Theoretical and Applied Genetics, 99, 1131-1142
• Prabhu, R.R., Njiti, V., J.E. Johnson, Schmidt M.E. Klein R.J. and D.A. Lightfoot. (1999) Selecting soybean cultivars for dual resistance to cyst nematode Sudden Death Syndrome with two DNA markers, Crop Science, 39 (4), 982-987
• McDaniel K., David Lightfoot. (1997) A gene specific cytokinin responsive mRNA in Phaseolus vulgaris leaves, Plant Physiology and Biochemistry, 35, 373-380
• Njiti, V.N, Suttner, R.J., Gray, L.E., Gibson, P.T. and D.A. Lightfoot (1997) Rate reducing resistance to Fusarium solani underlies field resistance to soybean sudden death syndrome (SDS), Crop Science, 37, 132-138
• Lightfoot DA, Baron AJ, Cock JM, Wootton JC (1992) A nitrate reductase gene of the cyanobacterium Synechococcus PCC6301 inferred by heterologous hybridization, cloning and targeted mutagenesis, Genetica, 85 (2), 107-17
• Lightfoot DA, McPherson MJ, Wootton JC (1989) Detection of a homologue to an E. coli glutamate synthase gene in a cyanobacterium, Fems Microbiology Letters, 48 (2), 129-32
• Lightfoot DA (1988) Magnesium-dependence of in vitro translation programmed by gene-specific mRNAs, Nucleic Acids Research, 16 (9), 4164
• Lightfoot, D.A., Baron, A.J. & Wootton, J.C. (1988) . Expression of Escherichia coli glutamate dehydrogenase in the cyanobacterium Synechococcus PCC6301 causes ammonia tolerance, Plant Molecular Biology, 11, 335-344