Nicole Frahm

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Fred Hutchinson Cancer Research Center
Clinical Research Division
Vaccine and Infectious Disease Institute
Assistant MemberAppointed: 2008
University of Washington
School of Public Health and Community Medicine
Department of Global Health
Assistant ProfessorAppointed: 2008
Professional Headshot of Nicole Frahm

Mailing Address

Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N.
LE-200
Seattle, Washington 98109-1024
United States

Contact Information

Phone: (206) 667-6268
nfrahm@fhcrc.org

Qualifications

Ph.D., University of Hamburg, Germany, Immunology, 2003.
M.S., University of Braunschweig, Germany, Study of Biotechnology, 1996.

Expertise and Research Interests

My research addresses the influence of HIV sequence diversity on its recognition by cytotoxic T lymphocytes, as well as the factors governing the recognition of sequence variants both in HIV-infected subjects and in vaccine trial participants. More recently, my research also includes the assessment of immune responses to viral vectors used as immunogens in HIV vaccine trials to help understand how pre-existing cellular immunity to the vector influences the quality of vaccine-induced immune responses. In my role as the Associate Laboratory Director for the HVTN, I provide leadership and scientific support for the HVTN Laboratories. This includes (but is not restricted to) oversight of the Endpoints Laboratory, which is responsible for the generation of validated immunogenicity data for all HVTN trials, and of the R&D Laboratory, which provides ancillary and exploratory data leading to a more complete view of the immune responses generated by HIV vaccines.

Keywords

COS Keywords:

Infectious Diseases Or Agents.

Languages

(Reading, Writing, Speaking)

German: (Fluent, Fluent, Fluent)
Portuguese (Brazilian): (Fluent, Fluent, Fluent)

Previous Positions

2006-2008, Instructor, Harvard University, Medical School
2006-2008, Assistant in Immunology, Massachusetts General Hospital, Partners AIDS Research Center, Infectious Diseases
2001-2006, Post-doctoral fellow, Harvard University, Medical School
2001-2006, Post-doctoral fellow, Massachusetts General Hospital, Partners AIDS Research Center, Infectious Diseases

Publications

  • Wang YE, Li B, Carlson JM, Streeck H, Gladden AD, Goodman R, Schneidewind A, Power KA, Toth I, Frahm N, Alter G, Brander C, Carrington M, Walker BD, Altfeld M, Heckerman D, Allen TM (Feb 2009) Protective HLA class I alleles that restrict acute-phase CD8+ T-cell responses are associated with viral escape mutations located in highly conserved regions of human immunodeficiency virus type 1., Journal of virology, 83 (4), 1845-55 Abstract
  • Dalmau J, Puertas MC, Azuara M, Mariņo A, Frahm N, Mothe B, Izquierdo-Useros N, Buzón MJ, Paredes R, Matas L, Allen TM, Brander C, Rodrigo C, Clotet B, Martinez-Picado J (Jan 2009) Contribution of immunological and virological factors to extremely severe primary HIV type 1 infection., Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 48 (2), 229-38 Abstract
  • Miura T, Brockman MA, Schneidewind A, Lobritz M, Pereyra F, Rathod A, Block BL, Brumme ZL, Brumme CJ, Baker B, Rothchild AC, Li B, Trocha A, Cutrell E, Frahm N, Brander C, Toth I, Arts EJ, Allen TM, Walker BD (Dec 2008) HLA-B57/B*5801 HIV-1 ELITE CONTROLLERS SELECT FOR RARE GAG VARIANTS ASSOCIATED WITH REDUCED VIRAL REPLICATION CAPACITY AND STRONG CTL RECOGNITION., Journal of virology Abstract
  • Rousseau CM, Daniels MG, Carlson JM, Kadie C, Crawford H, Prendergast A, Matthews P, Payne R, Rolland M, Raugi DN, Maust BS, Learn GH, Nickle DC, Coovadia H, Ndung'u T, Frahm N, Brander C, Walker BD, Goulder PJ, Bhattacharya T, Heckerman DE, Korber BT, Mullins JI (Jul 2008) HLA class I-driven evolution of human immunodeficiency virus type 1 subtype c proteome: immune escape and viral load., Journal of virology, 82 (13), 6434-46 Abstract
  • Brumme ZL, Tao I, Szeto S, Brumme CJ, Carlson JM, Chan D, Kadie C, Frahm N, Brander C, Walker B, Heckerman D, Harrigan PR (Jul 2008) Human leukocyte antigen-specific polymorphisms in HIV-1 Gag and their association with viral load in chronic untreated infection., AIDS (London, England), 22 (11), 1277-86 Abstract
  • Yerly D, Heckerman D, Allen TM, Chisholm JV, Faircloth K, Linde CH, Frahm N, Timm J, Pichler WJ, Cerny A, Brander C (Mar 2008) Increased cytotoxic T-lymphocyte epitope variant cross-recognition and functional avidity are associated with hepatitis C virus clearance., Journal of virology, 82 (6), 3147-53 Abstract
  • Frahm N, Nickle DC, Linde CH, Cohen DE, Zuņiga R, Lucchetti A, Roach T, Walker BD, Allen TM, Korber BT, Mullins JI, Brander C (Feb 2008) Increased detection of HIV-specific T cell responses by combination of central sequences with comparable immunogenicity., AIDS (London, England), 22 (4), 447-56 Abstract
  • Sidney J, Peters B, Frahm N, Brander C, Sette A (2008) HLA class I supertypes: a revised and updated classification., BMC immunology, 9, 1 Abstract
  • Frahm N, Kaufmann DE, Yusim K, Muldoon M, Kesmir C, Linde CH, Fischer W, Allen TM, Li B, McMahon BH, Faircloth KL, Hewitt HS, Mackey EW, Miura T, Khatri A, Wolinsky S, McMichael A, Funkhouser RK, Walker BD, Brander C, Korber BT (Nov 2007) Increased sequence diversity coverage improves detection of HIV-specific T cell responses., Journal of immunology (Baltimore, Md. : 1950), 179 (10), 6638-50 Abstract
  • Listgarten J, Frahm N, Kadie C, Brander C, Heckerman D (Oct 2007) A statistical framework for modeling HLA-dependent T cell response data., PLoS computational biology, 3 (10), 1879-86 Abstract
  • Frahm N, Yusim K, Suscovich TJ, Adams S, Sidney J, Hraber P, Hewitt HS, Linde CH, Kavanagh DG, Woodberry T, Henry LM, Faircloth K, Listgarten J, Kadie C, Jojic N, Sango K, Brown NV, Pae E, Zaman MT, Bihl F, Khatri A, John M, Mallal S, Marincola FM, Walker BD, Sette A, Heckerman D, Korber BT, Brander C (Sep 2007) Extensive HLA class I allele promiscuity among viral CTL epitopes., European journal of immunology, 37 (9), 2419-33 Abstract
  • Meier A, Alter G, Frahm N, Sidhu H, Li B, Bagchi A, Teigen N, Streeck H, Stellbrink HJ, Hellman J, van Lunzen J, Altfeld M (Aug 2007) MyD88-dependent immune activation mediated by human immunodeficiency virus type 1-encoded Toll-like receptor ligands., Journal of virology, 81 (15), 8180-91 Abstract
  • Brainard DM, Tager AM, Misdraji J, Frahm N, Lichterfeld M, Draenert R, Brander C, Walker BD, Luster AD (Aug 2007) Decreased CXCR3+ CD8 T cells in advanced human immunodeficiency virus infection suggest that a homing defect contributes to cytotoxic T-lymphocyte dysfunction., Journal of virology, 81 (16), 8439-50 Abstract
  • Timm J, Li B, Daniels MG, Bhattacharya T, Reyor LL, Allgaier R, Kuntzen T, Fischer W, Nolan BE, Duncan J, Schulze zur Wiesch J, Kim AY, Frahm N, Brander C, Chung RT, Lauer GM, Korber BT, Allen TM (Aug 2007) Human leukocyte antigen-associated sequence polymorphisms in hepatitis C virus reveal reproducible immune responses and constraints on viral evolution., Hepatology (Baltimore, Md.), 46 (2), 339-49 Abstract
  • Brumme ZL, Brumme CJ, Heckerman D, Korber BT, Daniels M, Carlson J, Kadie C, Bhattacharya T, Chui C, Szinger J, Mo T, Hogg RS, Montaner JS, Frahm N, Brander C, Walker BD, Harrigan PR (Jul 2007) Evidence of differential HLA class I-mediated viral evolution in functional and accessory/regulatory genes of HIV-1., PLoS pathogens, 3 (7), e94 Abstract
  • Schaubert KL, Price DA, Frahm N, Li J, Ng HL, Joseph A, Paul E, Majumder B, Ayyavoo V, Gostick E, Adams S, Marincola FM, Sewell AK, Altfeld M, Brenchley JM, Douek DC, Yang OO, Brander C, Goldstein H, Kan-Mitchell J (Jun 2007) Availability of a diversely avid CD8+ T cell repertoire specific for the subdominant HLA-A2-restricted HIV-1 Gag p2419-27 epitope., Journal of immunology (Baltimore, Md. : 1950), 178 (12), 7756-66 Abstract
  • Assarsson E, Sidney J, Oseroff C, Pasquetto V, Bui HH, Frahm N, Brander C, Peters B, Grey H, Sette A (Jun 2007) A quantitative analysis of the variables affecting the repertoire of T cell specificities recognized after vaccinia virus infection., Journal of immunology (Baltimore, Md. : 1950), 178 (12), 7890-901 Abstract
  • Frahm N, Brander C (Mar 2007) HIV Viral Diversity and Escape from Cellular Immunity., Current infectious disease reports, 9 (2), 161-6 Abstract
  • Bhattacharya T, Daniels M, Heckerman D, Foley B, Frahm N, Kadie C, Carlson J, Yusim K, McMahon B, Gaschen B, Mallal S, Mullins JI, Nickle DC, Herbeck J, Rousseau C, Learn GH, Miura T, Brander C, Walker B, Korber B (Mar 2007) Founder effects in the assessment of HIV polymorphisms and HLA allele associations., Science (New York, N.Y.), 315 (5818), 1583-6 Abstract
  • Yu XG, Lichterfeld M, Chetty S, Williams KL, Mui SK, Miura T, Frahm N, Feeney ME, Tang Y, Pereyra F, Labute MX, Pfafferott K, Leslie A, Crawford H, Allgaier R, Hildebrand W, Kaslow R, Brander C, Allen TM, Rosenberg ES, Kiepiela P, Vajpayee M, Goepfert PA, Altfeld M, Goulder PJ, Walker BD (Feb 2007) Mutually exclusive T-cell receptor induction and differential susceptibility to human immunodeficiency virus type 1 mutational escape associated with a two-amino-acid difference between HLA class I subtypes., Journal of virology, 81 (4), 1619-31 Abstract
  • Rolland M, Nickle DC, Deng W, Frahm N, Brander C, Learn GH, Heckerman D, Jojic N, Jojic V, Walker BD, Mullins JI (2007) Recognition of HIV-1 peptides by host CTL is related to HIV-1 similarity to human proteins., PLoS ONE, 2 (9), e823 Abstract
  • Brander C, Frahm N, Walker BD (Aug 2006) The challenges of host and viral diversity in HIV vaccine design., Current opinion in immunology, 18 (4), 430-7 Abstract
  • Lichterfeld M, Williams KL, Mui SK, Shah SS, Mothe BR, Sette A, Kim A, Johnston MN, Burgett N, Frahm N, Cohen D, Brander C, Rosenberg ES, Walker BD, Altfeld M, Yu XG (Jul 2006) T cell receptor cross-recognition of an HIV-1 CD8+ T cell epitope presented by closely related alleles from the HLA-A3 superfamily., International immunology, 18 (7), 1179-88 Abstract
  • Bihl F, Frahm N, Di Giammarino L, Sidney J, John M, Yusim K, Woodberry T, Sango K, Hewitt HS, Henry L, Linde CH, Chisholm JV, Zaman TM, Pae E, Mallal S, Walker BD, Sette A, Korber BT, Heckerman D, Brander C (Apr 2006) Impact of HLA-B alleles, epitope binding affinity, functional avidity, and viral coinfection on the immunodominance of virus-specific CTL responses., Journal of immunology (Baltimore, Md. : 1950), 176 (7), 4094-101 Abstract
  • Kavanagh DG, Kaufmann DE, Sunderji S, Frahm N, Le Gall S, Boczkowski D, Rosenberg ES, Stone DR, Johnston MN, Wagner BS, Zaman MT, Brander C, Gilboa E, Walker BD, Bhardwaj N (Mar 2006) Expansion of HIV-specific CD4+ and CD8+ T cells by dendritic cells transfected with mRNA encoding cytoplasm- or lysosome-targeted Nef., Blood, 107 (5), 1963-9 Abstract
  • Zuņiga R, Lucchetti A, Galvan P, Sanchez S, Sanchez C, Hernandez A, Sanchez H, Frahm N, Linde CH, Hewitt HS, Hildebrand W, Altfeld M, Allen TM, Walker BD, Korber BT, Leitner T, Sanchez J, Brander C (Mar 2006) Relative dominance of Gag p24-specific cytotoxic T lymphocytes is associated with human immunodeficiency virus control., Journal of virology, 80 (6), 3122-5 Abstract
  • Frahm N, Kiepiela P, Adams S, Linde CH, Hewitt HS, Sango K, Feeney ME, Addo MM, Lichterfeld M, Lahaie MP, Pae E, Wurcel AG, Roach T, St John MA, Altfeld M, Marincola FM, Moore C, Mallal S, Carrington M, Heckerman D, Allen TM, Mullins JI, Korber BT, Goulder PJ, Walker BD, Brander C (Feb 2006) Control of human immunodeficiency virus replication by cytotoxic T lymphocytes targeting subdominant epitopes., Nature immunology, 7 (2), 173-8 Abstract
  • Woodberry T, Suscovich TJ, Henry LM, Davis JK, Frahm N, Walker BD, Scadden DT, Wang F, Brander C (Nov 2005) Differential targeting and shifts in the immunodominance of Epstein-Barr virus--specific CD8 and CD4 T cell responses during acute and persistent infection., The Journal of infectious diseases, 192 (9), 1513-24 Abstract
  • Allen TM, Yu XG, Kalife ET, Reyor LL, Lichterfeld M, John M, Cheng M, Allgaier RL, Mui S, Frahm N, Alter G, Brown NV, Johnston MN, Rosenberg ES, Mallal SA, Brander C, Walker BD, Altfeld M (Oct 2005) De novo generation of escape variant-specific CD8+ T-cell responses following cytotoxic T-lymphocyte escape in chronic human immunodeficiency virus type 1 infection., Journal of virology, 79 (20), 12952-60 Abstract
  • Frahm N, Adams S, Kiepiela P, Linde CH, Hewitt HS, Lichterfeld M, Sango K, Brown NV, Pae E, Wurcel AG, Altfeld M, Feeney ME, Allen TM, Roach T, St John MA, Daar ES, Rosenberg E, Korber B, Marincola F, Walker BD, Goulder PJ, Brander C (Aug 2005) HLA-B63 presents HLA-B57/B58-restricted cytotoxic T-lymphocyte epitopes and is associated with low human immunodeficiency virus load., Journal of virology, 79 (16), 10218-25 Abstract
  • Bihl FK, Loggi E, Chisholm JV, Hewitt HS, Henry LM, Linde C, Suscovich TJ, Wong JT, Frahm N, Andreone P, Brander C (May 2005) Simultaneous assessment of cytotoxic T lymphocyte responses against multiple viral infections by combined usage of optimal epitope matrices, anti- CD3 mAb T-cell expansion and "RecycleSpot", Journal of translational medicine, 3 (1), 20 Abstract
  • Altfeld M, Allen TM, Kalife ET, Frahm N, Addo MM, Mothe BR, Rathod A, Reyor LL, Harlow J, Yu XG, Perkins B, Robinson LK, Sidney J, Alter G, Lichterfeld M, Sette A, Rosenberg ES, Goulder PJ, Brander C, Walker BD (Apr 2005) The majority of currently circulating human immunodeficiency virus type 1 clade B viruses fail to prime cytotoxic T-lymphocyte responses against an otherwise immunodominant HLA-A2-restricted epitope: implications for vaccine design., Journal of virology, 79 (8), 5000-5 Abstract
  • Altfeld M, Allen TM, Kalife ET, Frahm N, Addo MM, Mothe BR, Rathod A, Reyor LL, Harlow J, Yu XG, Perkins B, Robinson LK, Sidney J, Alter G, Lichterfeld M, Sette A, Rosenberg ES, Goulder PJ, Brander C, Walker BD (Apr 2005) The majority of currently circulating human immunodeficiency virus type 1 clade B viruses fail to prime cytotoxic T-lymphocyte responses against an otherwise immunodominant HLA-A2-restricted epitope: implications for vaccine design., Journal of virology, 79 (8), 5000-5 Abstract
  • Hess C, Means TK, Autissier P, Woodberry T, Altfeld M, Addo MM, Frahm N, Brander C, Walker BD, Luster AD (Dec 2004) IL-8 responsiveness defines a subset of CD8 T cells poised to kill., Blood, 104 (12), 3463-71 Abstract
  • Kaufmann DE, Bailey PM, Sidney J, Wagner B, Norris PJ, Johnston MN, Cosimi LA, Addo MM, Lichterfeld M, Altfeld M, Frahm N, Brander C, Sette A, Walker BD, Rosenberg ES (May 2004) Comprehensive analysis of human immunodeficiency virus type 1-specific CD4 responses reveals marked immunodominance of gag and nef and the presence of broadly recognized peptides., Journal of virology, 78 (9), 4463-77 Abstract
  • Yu XG, Addo MM, Perkins BA, Wej F, Rathod A, Geer SC, Parta M, Cohen D, Stone DR, Russell CJ, Tanzi G, Mei S, Wurcel AG, Frahm N, Lichterfeld M, Heath L, Mullins JI, Marincola F, Goulder PJ, Brander C, Allen T, Cao Y, Walker BD, Altfeld M (May 2004) Differences in the expressed HLA class I alleles effect the differential clustering of HIV type 1-specific T cell responses in infected Chinese and caucasians., AIDS research and human retroviruses, 20 (5), 557-64 Abstract
  • Frahm N, Korber BT, Adams CM, Szinger JJ, Draenert R, Addo MM, Feeney ME, Yusim K, Sango K, Brown NV, SenGupta D, Piechocka-Trocha A, Simonis T, Marincola FM, Wurcel AG, Stone DR, Russell CJ, Adolf P, Cohen D, Roach T, StJohn A, Khatri A, Davis K, Mullins J, Goulder PJ, Walker BD, Brander C (Mar 2004) Consistent cytotoxic-T-lymphocyte targeting of immunodominant regions in human immunodeficiency virus across multiple ethnicities., Journal of virology, 78 (5), 2187-200 Abstract
  • Altfeld M, van Lunzen J, Frahm N, Yu XG, Schneider C, Eldridge RL, Feeney ME, Meyer-Olson D, Stellbrink HJ, Walker BD (Mar 2002) Expansion of pre-existing, lymph node-localized CD8+ T cells during supervised treatment interruptions in chronic HIV-1 infection., The Journal of clinical investigation, 109 (6), 837-43 Abstract
  • Jacobs T, Darji A, Frahm N, Rohde M, Wehland J, Chakraborty T, Weiss S (Jun 1998) Listeriolysin O: cholesterol inhibits cytolysis but not binding to cellular membranes., Molecular microbiology, 28 (6), 1081-9 Abstract
  • Paglia P, Arioli I, Frahm N, Chakraborty T, Colombo MP, Guzmān CA (Jun 1997) The defined attenuated Listeria monocytogenes delta mp12 mutant is an effective oral vaccine carrier to trigger a long-lasting immune response against a mouse fibrosarcoma., European journal of immunology, 27 (6), 1570-5 Abstract

Profile Details

Last Updated: 6/1/2009

COS Expertise ID #1305163
Reference this profile directly: http://myprofile.cos.com/nfrahm