Dr. Owen Mitch Griffith

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Ultra Speed & High/SuperSpeed Centrifuge ConsultantAppointed: 2006
Professional Headshot of Owen Mitch Griffith

Mailing Address

2706 Pruneridge Ave
Santa Clara, California 95051
United States

Contact Information

Phone: (408) 248-8334
Fax: (408) 248-8099
Mobile: (408) 839-7783
ogriffith@pacbell.net

Qualifications

Ph.D., California Western University (now California Coast University), Molecular Biology and Biochemistry, 1977.
M.S., New York Medical College, Medical Microbiology, 1961.
Diploma, University of Geneva, Biochemistry, 1959.
B.Sc., Morgan State University, Chemistry, 1957.

Expertise and Research Interests

Design carbon fiber swinging bucket rotors for 96 well micro plates for PCR studies to clean-up automated DNA sequencing studies using 96 well format plates for cell culture studies.

Designed Centrifuge methods for Carbon Fiber Rotors to enable researchers to prepare and purify large and small volumes of proteins, antibodies, subcellular organelles, nucleic acids (DNA & RNA), viruses, mammalian cells and bacteria from culture for vaccine preparation.

Assist Scientists in choosing the best centrifuges and accessories including rotors, for run time reduction and throughput for their centrifugal separations. Presented Seminars worldwide at Universities and institutions on topics involving preparation and purification of nucleic Acids, viruses and subcellular organelles by High Speed and Ultra speed centrifugation methods.

Other Expertise

During the Period from 1962 to 1986 was employed by Beckman Instruments, Spinco Division as the Principal Applications Scientist. In addition to doing research in new methods for their existing centrifuges, assisted Scientists in choosing the best centrifuges and accessories including rotors for run time reduction and methods for their centrifugal separations. Developed methods for using Light weight Carbon Fiber rotors instead of metallic Rotors for all existing centrifuges.

Future Research

Develop new methods for using lightweight, non corroding and high energy Carbon Fiber Rotors for high/super speed, ultra speed and table top centrifuges to obtain better separations and purifucation of samples collected by centrifugation.

Industrial Relevance

Metallic Rotors are manufactured from titanium and aluminum alloys. The weight of these rotors often cause injury to some male and female technicians who must load and unload these rotors into the centrifuge during their research practices.

Light weight of Carbon Fiber Centrifuge Rotors permit rapid acceleration and deceleration so that research times are reduced thus giving greater throughput and energy savings for sample preparation and particle separation.

Keywords

COS Keywords:

Biochemistry, Molecular Biology.

Additional Terms:

Centrifugal Separations in Molecular Biology, Density Gradient Centrifugation, FIBERLite Carbon Fiber Rotors, Molecular Biology & Biochemistry.

Memberships

American Association for the Advancement of Science
American Chemical Society
American Society for Biochemistry and Molecular Biology
American Society for Microbiology

Honors and Awards

1971-1986, Professional Chemist Acredited, American Institute of Chemists, California Western University, Applied Research in Preparative & High/Super Speed Centrifuges

Previous Positions

2001-2006, Consultant, FIBERLite Centrifuge Inc., Research & Applications
1987-2001, Director, Composite Rotor, Inc., Applications Research
1962-1987, Principal Applications Scientist, Beckman Instruments, Analytical, Preparative Ultra and High/Super Speed Centrifuges, Research & Applications, Spinco

Publications

  • Owen Mitch Griffith Ph.D, Density Gradient Separations in Vertical tube, Near Vertical Tube FIBERLite Fixed Angle, and Swinging Bucket Rotors: A comparative Study., Applications Article # 11342, 422 Aldo Ave., Santa Clara CA 95054. U.S.A, FIBERLite Centrifuge Inc., 20 Mar 2007
  • Owen Mitch Griffith Ph.D (2006) Practical Techniques for Centrifugal Separations, First Edition, Vol. No. 1, 422 Aldo Ave, Santa Clara CA. 95051, FIBERLite Centrifuge Inc, 107 pages
  • Griffith, OM, Pelleting Bacteria using FIBERLite Large Volume Rotors - A Comparative Study, 1, 2, January 2004
  • Griffith OM, Cesium chloride gradients in carbon fiber fixed angle rotor for purification of viruses, organelles, and plasmid DNA, American Biotechnology Laboratory, 9(8), 24-26, September 1991
  • Griffith OM, Large-scale isolation of plasmid DNA using high speed centrifugation methods, Biotechniques, 6(8), 725-7, September 1988 Abstract
  • Griffith, OM, Techniques of Preparative Zonal and Continuous Flow Ultracentrifugation, SBU8.94088, 50, Jan 1986
  • Griffith OM, Rapid density gradient centrifugation using short column techniques, Analytical Biochemistry, 90(1), 435-43, 1978
  • Griffith OM, Separation of T and B cells from human peripheral blood by centrifugal elutriation, Analytical Biochemistry, 87(1), 97-107, 1978
  • Griffith OM, Wright H, Resolution of components from rat liver homogenate in reorienting density gradients, Analytical Biochemistry, 47(2), 575-83, June 1972 Abstract
  • Lust J, Griffith OM, Conditions of phenol extraction of RNA from rat liver subcellular cytoplasmic fractions. I. Effect of a precipitation of microsomal RNA on its purity, Preparative Biochemistry, 2(2), 113-24, 1972 Abstract
  • Lust J, Griffith OM, Conditions of phenol extraction of RNA from rat liver subcellular cytoplasmic fractions. II. Effect of a glycine NaOH buffer on the separation of polysomal RNA, Preparative Biochemistry, 2(2), 125-37, 1972 Abstract
  • Griffith OM, Gropper L, A Schlieren optics accessory for use with the preparative ultracentrifuge, Analytical Biochemistry, 31(1), 218-26, 1969 Abstract
  • Jolley WB, Allen HW, Griffith OM, Ultracentrifugation using acrylamide gel, Analytical Biochemistry, 21(3), 454-61, December 1967 Abstract
  • Griffith OM, Gordon MJ, Patterson J, Micule spheres used as density markers for gradient column centrifugation, Analytical Biochemistry, 21(2), 329-32, November 1967 Abstract
  • Griffith OM, Rapid sedimentation equilibrium in the ultracentrifuge using a modified synthetic boundary cell with step concentrations, Analytical Biochemistry, 19(2), 243-8, May 1967 Abstract
  • Gropper L, Griffith O, Band-forming caps for the layering of sample in swinging-bucket rotors, Analytical Biochemistry, 16(1), 171-6, July 1966 Abstract

Profile Details

Last Updated: 10/25/2007

COS Expertise ID #324294
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