Philippe Soriano

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Mount Sinai School of Medicine of New York University
Developmental and Regenerative Biology
Professor
Professional Headshot of Philippe Soriano

Mailing Address

Department of Developmental & Regenerative Biology
Mount Sinai School of Medicine
1 Gustave L. Levy Place
Box 1020
New York, New York 10029
United States

Contact Information

Phone: (212) 241-4552
Fax: (212) 860-9279
philippe.soriano@mssm.edu
http://www.drbsinai.org/pc/soriano.html

Qualifications

D.Sc., University of Paris, 1982.
Ph.D., University of Paris, 1978.

Expertise and Research Interests

Research in this laboratory is centered on the genetic analysis of mouse development, with a particular emphasis on genes implicated in growth factor signaling pathways. Using gene targeting in ES cells, we have shown that platelet derived growth factors (PDGF) signaling is critical for normal development of the vasculature, cranial and cardiac neural crest cells, the somites, and oligodendrocytes. We have studied the role of fibroblast growth factor (FGF) signaling in mesodermal and neural development. These studies are complemented by temporal and tissue specific mutagenesis using site directed recombinases. We are also developing mice carrying small mutations that prevent various effectors from docking to the receptors, to unravel the role of various signal transduction pathways in a physiological context. Last, we are creating activating mutations in PDGF receptors that might provide information on the role of this signaling pathway in the control of normal cell proliferation and cancer.

To identify downstream targets of growth factor signaling and their physiological role, we are using gene trap mutagenesis in embryonic stem (ES) cells. In this approach, a promoterless reporter gene (for instance encoding beta-galactosidase) is introduced in ES cells. Expression of the reporter gene only occurs if it has disrupted an endogenous locus, typically leading to a null allele. Using a platform in which gene trap mutagenesis is coupled to DNA microarrays, we have identified and mutated numerous genes whose expression is regulated by PDGF. We are also performing gain of function screens on sensitized backgrounds to identify new signal transduction pathways that control neural crest cell behavior.

We are studying the involvement other signaling pathways, in particular ephrin signaling, that regulate neural crest cell development. Eph receptors and ephrins function as an unusual receptor/ligand pair that can activate both forward signaling to Eph receptors as well as reverse signaling through the ephrin GPI-anchor or cytoplasmic domain. We have shown that ephrin-B1 regulates cranial neural crest development through reverse signaling and that ephrin-B2 regulates trunk neural crest development through forward signaling. We have also shown that ephrin signaling can mediate boundary formation during development by regulating gap junction communication.

For more information about our research, please consult our lab web site: http://www.drbsinai.org/pc/soriano.html

Keywords

COS Keywords:

Animal Genetics or Breeding, Cell Proliferation, Developmental Biology, Extracellular Matrix, Genetics, Growth Factors, Mutation, Signal Transduction, Stem Cells.

Additional Terms:

Cell Signaling, Developmental Biology, Neural Crest.

Publications

  • Bush JO, Soriano P (Jul 2009) Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism., Genes & development, 23 (13), 1586-99 Abstract
  • Olson LE, Soriano P (Feb 2009) Increased PDGFRalpha activation disrupts connective tissue development and drives systemic fibrosis., Developmental cell, 16 (2), 303-13 Abstract
  • Schmahl J, Rizzolo K, Soriano P (Dec 2008) The PDGF signaling pathway controls multiple steroid-producing lineages., Genes & development, 22 (23), 3255-67 Abstract
  • Qiu R, Wang X, Davy A, Wu C, Murai K, Zhang H, Flanagan JG, Soriano P, Lu Q (Jun 2008) Regulation of neural progenitor cell state by ephrin-B., The Journal of cell biology, 181 (6), 973-83 Abstract
  • Davy A, Soriano P (Apr 2007) Ephrin-B2 forward signaling regulates somite patterning and neural crest cell development., Developmental biology, 304 (1), 182-93 Abstract
  • Raymond CS, Soriano P (2007) High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells., PloS one, 2 (1), e162 Abstract
  • Schmahl J, Raymond CS, Soriano P (Jan 2007) PDGF signaling specificity is mediated through multiple immediate early genes., Nature genetics, 39 (1), 52-60 Abstract
  • Davy A, Bush JO, Soriano P (Oct 2006) Inhibition of gap junction communication at ectopic Eph/ephrin boundaries underlies craniofrontonasal syndrome., PLoS biology, 4 (10), e315 Abstract
  • Raymond CS, Soriano P (Sep 2006) Engineering mutations: deconstructing the mouse gene by gene., Developmental dynamics : an official publication of the American Association of Anatomists, 235 (9), 2424-36 Abstract
  • Hoch RV, Soriano P (Feb 2006) Context-specific requirements for Fgfr1 signaling through Frs2 and Frs3 during mouse development., Development (Cambridge, England), 133 (4), 663-73 Abstract
  • Xu X, Bringas P Jr, Soriano P, Chai Y (Jan 2005) PDGFR-alpha signaling is critical for tooth cusp and palate morphogenesis., Developmental Dynamics : an Official Publication of the American Association of Anatomists., 232 (1), 75-84 Abstract
  • Davy A, Soriano P (Jan 2005) Ephrin signaling in vivo: look both ways., Developmental Dynamics : an Official Publication of the American Association of Anatomists., 232 (1), 1-10 Abstract
  • Ding H, Wu X, Bostrom H, Kim I, Wong N, Tsoi B, O'Rourke M, Koh GY, Soriano P, Betsholtz C, Hart TC, Marazita ML, Field LL, Tam PP, Nagy A, A specific requirement for PDGF-C in palate formation and PDGFR-alpha signaling, Nature Genetics, 36(10), 1111-6, Oct 2004 Abstract
  • Austin CP, Battey JF, Bradley A, Bucan M, Capecchi M, Collins FS, Dove WF, Duyk G, Dymecki S, Eppig JT, Grieder FB, Heintz N, Hicks G, Insel TR, Joyner A, Koller BH, Lloyd KC, Magnuson T, Moore MW, Nagy A, Pollock JD, Roses AD, Sands AT, Seed B, Skarnes W, The knockout mouse project, Nature Genetics, 36(9), 921-4, Sep 2004 Abstract
  • Skarnes WC, von Melchner H, Wurst W, Hicks G, Nord AS, Cox T, Young SG, Ruiz P, Soriano P, Tessier-Lavigne M, Conklin BR, Stanford WL, Rossant J, International Gene Trap Consortium, A public gene trap resource for mouse functional genomics, Nature Genetics, 36(6), 543-4, 2004 Abstract
  • Aubin J, Davy A, Soriano P, In vivo convergence of BMP and MAPK signaling pathways: impact of differential Smad1 phosphorylation on development and homeostasis, Genes & Development, 18(12), 1482-94, June 2004 Abstract
  • Davy A, Aubin J, Soriano P, Ephrin-B1 forward and reverse signaling are required during mouse development, Genes & Development, 18(5), 572-83, 2004 Abstract
  • Chen WV, Delrow J, Corrin PD, Frazier JP, Soriano P, Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis, Nature Genetics, 36(3), 304-12, 2004 Abstract
  • Tallquist MD, French WJ, Soriano P (Nov 2003) Additive effects of PDGF receptor beta signaling pathways in vascular smooth muscle cell development., Plos Biology, 1 (2), E52 Abstract
  • Hoch RV, Soriano P, Roles of PDGF in animal development, Development (Cambridge, England), 130(20), 4769-84, October 2003
  • Awatramani R, Soriano P, Rodriguez C, Mai JJ, Dymecki SM, Cryptic boundaries in roof plate and choroid plexus identified by intersectional gene activation, Nature Genetics, 35(1), 70-5, 2003 Abstract
  • Hamilton TG, Klinghoffer RA, Corrin PD, Soriano P, Evolutionary divergence of platelet-derived growth factor alpha receptor signaling mechanisms, Molecular and Cellular Biology, 23(11), 4013-25, June 2003 Abstract
  • Tallquist MD, Soriano P, Cell autonomous requirement for PDGFRalpha in populations of cranial and cardiac neural crest cells, Development (Cambridge, England), 130(3), 507-18, February 2003
  • Chen WV, Soriano P, Gene trap mutagenesis in embryonic stem cells, Methods in Enzymology, 365, 367-86, 2003 Abstract
  • Hildebrand JD, Soriano P, Overlapping and unique roles for C-terminal binding protein 1 (CtBP1) and CtBP2 during mouse development, Molecular and Cellular Biology, 22(15), 5296-307, Aug 2002 Abstract
  • Klinghoffer RA, Hamilton TG, Hoch R, Soriano P, An allelic series at the PDGFalphaR locus indicates unequal contributions of distinct signaling pathways during development, Developmental Cell, 2(1), 103-13, January 2002 Abstract
  • Komada M, Soriano P, [Beta]IV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier, Journal of Cell Biology, 156(2), 337-48, January 2002 Abstract
  • Awatramani R, Soriano P, Mai JJ, Dymecki S, An Flp indicator mouse expressing alkaline phosphatase from the ROSA26 locus, Nature Genetics, 29(3), 257-9, November 2001 Abstract
  • Medico E, Gambarotta G, Gentile A, Comoglio PM, Soriano P, A gene trap vector system for identifying transcriptionally responsive genes, Nature Biotechnology, 19(6), 579-82, June 2001 Abstract
  • Klinghoffer RA, Mueting-Nelsen PF, Faerman A, Shani M, Soriano P, The two PDGF receptors maintain conserved signaling in vivo despite divergent embryological functions, Molecular Cell, 7(2), 343-54, February 2001 Abstract
  • Nadeau JH, Balling R, Barsh G, et al, Sequence interpretation. Functional annotation of mouse genome sequences, Science, 291(5507), 1251-5, 2001
  • Tallquist MD, Klinghoffer RA, Heuchel R, Mueting-Nelsen PF, Corrin PD, Heldin CH, Johnson RJ, Soriano P, Retention of PDGFR-beta function in mice in the absence of phosphatidylinositol 3'-kinase and phospholipase Cgamma signaling pathways, Genes and Development, 14(24), 3179-90, 2000 Abstract
  • Tallquist MD, Weismann KE, Hellstrom M, Soriano P, Early myotome specification regulates PDGFA expression and axial skeleton development, Development, 127(23), 5059-70, December 2000 Abstract
  • Farley FW, Soriano P, Steffen LS, Dymecki SM, Widespread recombinase expression using FLPeR (flipper) mice, Genesis, 28(3-4), 106-10, 2000 Abstract
  • Komada M, McLean DJ, Griswold MD, Russell LD, Soriano P, E-MAP-115, encoding a microtubule-associated protein, is a retinoic acid-inducible gene required for spermatogenesis, Genes and Development, 14(11), 1332-42, June 2000 Abstract
  • Klesert TR, Cho DH, Clark JI, Maylie J, Adelman J, Snider L, Yuen EC, Soriano P, Tapscott SJ, Mice deficient in Six5 develop cataracts: Implications for myotonic dystrophy, Nature Genetics, 25(1), 105-9, May 2000
  • Li X, Ponten A, Aase K, Karlsson L, Abramsson A, Uutela M, Backstrom G, Hellstrom M, Bostrom H, Li H, Soriano P, Betsholtz C, Heldin CH, Alitalo K, Ostman A, Eriksson U, PDGF-C is a new protease-activated ligand for the PDGF alpha-receptor, Nature Cell Biology, 2(5), 302-9, May 2000 Abstract
  • Jiang X, Rowitch DH, Soriano P, McMahon AP, Sucov HM, Fate of the mammalian cardiac neural crest, Development, 127(8), 1607-16, April 2000 Abstract
  • Chai Y, Jiang X, Ito Y, Bringas P Jr, Han J, Rowitch DH, Soriano P, McMahon AP, Sucov HM, Fate of the mammalian cranial neural crest during tooth and mandibular morphogenesis, Development, 127(8), 1671-9, April 2000 Abstract
  • Tallquist MD, Soriano P, Epiblast-restricted Cre expression in MORE mice: A tool to distinguish embryonic vs. extra-embryonic gene function, 26(2), 113-5, February 2000
  • Tallquist MD, Soriano P, Klinghoffer RA, Growth factor signaling pathways in vascular development, Oncogene, 18(55), 7917-32, December 1999 Abstract
  • Davy A, Gale NW, Murray EW, Klinghoffer RA, Soriano P, Feuerstein C, Robbins SM, Compartmentalized signaling by GPI-anchored ephrin-A5 requires the Fyn tyrosine kinase to regulate cellular adhesion, Genes and Development, 13(23), 3125-35, December 1999 Abstract
  • Hildebrand JD, Soriano P, Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice, Cell, 99(5), 485-97, November 1999 Abstract
  • Heuchel R, Berg A, Tallquist M, Ahlen K, Reed RK, Rubin K, Claesson-Welsh L, Heldin CH, Soriano P, Platelet-derived growth factor beta receptor regulates interstitial fluid homeostasis through phosphatidylinositol-3' kinase signaling, Proceedings of the National Academy of Sciences (USA), 96(20), 11410-5, September 1999 Abstract
  • Ohlsson R, Falck P, Hellstrom M, Lindahl P, Bostrom H, Franklin G, Ahrlund-Richter L, Pollard J, Soriano P, Betsholtz C, PDGFB regulates the development of the labyrinthine layer of the mouse fetal placenta, Developmental Biology, 212(1), 124-36, August 1999 Abstract
  • Komada M, Soriano P, Hrs, a FYVE finger protein localized to early endosomes, is implicated in vesicular traffic and required for ventral folding morphogenesis, Genes and Development, 13(11), 1475-85, June 1999 Abstract
  • Klinghoffer RA, Sachsenmaier C, Cooper JA, Soriano P, Src family kinases are required for integrin but not PDGFR signal transduction, Embo Journal, 18(9), 2459-71, May 1999 Abstract
  • Wilde A, Beattie EC, Lem L, Riethof DA, Liu SH, Mobley WC, Soriano P, Brodsky FM, EGF receptor signaling stimulates SRC kinase phosphorylation of clathrin, influencing clathrin redistribution and EGF uptake, Cell, 96(5), 677-87, March 1999 Abstract
  • Lanier LM, Gates MA, Witke W, Menzies AS, Wehman AM, Macklis JD, Kwiatkowski D, Soriano P, Gertler FB, Mena is required for neurulation and commissure formation, Neuron, 22(2), 313-25, February 1999 Abstract
  • Soriano P, Generalized lacZ expression with the ROSA26 Cre reporter strain, Nature Genetics, 21(1), 70-1, Jan 1999 Abstract
  • Gallagher E, Howell BW, Soriano P, Cooper JA, Hawkes R, Cerebellar abnormalities in the disabled (mdab1-1) mouse, Journal of Comparative Neurology, 402(2), 238-51, December 1998 Abstract
  • Lindahl P, Hellstrom M, Kalen M, Karlsson L, Pekny M, Pekna M, Soriano P, Betsholtz C, Paracrine PDGF-B/PDGF-Rbeta signaling controls mesangial cell development in kidney glomeruli, Development, 125(17), 3313-22, September 1998 Abstract
  • Fransen E, D'Hooge R, Van Camp G, Verhoye M, Sijbers J, Reyniers E, Soriano P, Kamiguchi H, Willemsen R, Koekkoek SK, De Zeeuw CI, De Deyn PP, Van der Linden A, Lemmon V, Kooy RF, Willems PJ, L1 knockout mice show dilated ventricles, vermis hypoplasia and impaired exploration patterns, Human Molecular Genetics, 7(6), 999-1009, June 1998 Abstract
  • Crosby J R, Seifert R A, Soriano P, Bowen-Pope D F, Chimaeric analysis reveals role of Pdgf receptors in all muscle lineages, Nature Genetics, 18, 385-388, 1998 Abstract
  • Soriano P, The PDGF alpha receptor is required for neural crest cell development and for normal patterning of the somites, Development, 124, 2691-2700, 1997 Abstract
  • Zambrowicz BP, Imamoto A, Fiering S, Herzenberg LA, Kerr WG, Soriano P, Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells, Proceedings of the National Academy of Sciences (USA), 94, 3789-3794, 1997 Abstract
  • Lowell CA, Niwa M, Soriano P, Varmus HE, Deficiency of the Hck and Src tyrosine kinases results in extreme levels of extramedullary hematopoiesis, Blood, 87(5), 1780-92, March 1996 Abstract
  • Gertler FB, Niebuhr K, Reinhard M, Wehland J, Soriano P, Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics, Cell, 87, 227-239, 1996 Abstract
  • Lowell CA, Soriano P, Knockouts of Src-family kinases: Stiff bones, wimpy T cells, and bad memories, Genes and Development, 10, 1845-2857, 1996

Profile Details

Last Updated: 7/7/2009

COS Expertise ID #441429
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