Dr. Richard Brian Presland

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University of Washington
School of Dentistry
Medicine
Dermatology
Research Associate ProfessorAppointed: 2001
University of Washington
School of Dentistry
Oral Biology
Research Associate ProfessorAppointed: 2001
Professional Headshot of Richard Brian Presland

Mailing Address

Box 357132
Department of Oral Biology
Seattle, Washington 98195
United States

Contact Information

Phone: (206) 543-1595
Fax: (206) 685-3162
rp@u.washington.edu

Qualifications

Ph.D., Adelaide University, Biochemistry/Molecular Biology, 1987.
M.Sc., University of Otago, Biochemistry, 1981.
B.Sc., University of Otago, Biochemistry, 1979.

Expertise and Research Interests

Epithelial tissues, such as those that line the oral cavity and skin, function to protect animals from physical, chemical, and microbial attack, and dessication. They are essential for survival. During keratinocyte differentiation, living cells undergo desquamation to form the dead, cornified cells at the epithelial surface. These "squames" comprise a major part of the epithelial barrier and are continuously being renewed. Our research focuses in two areas important to this process: S100 calcium-binding proteins (including profilaggrin) that function in many calcium-dependent processes in cells; and caspase-14, an epithelial protease that is a member of the caspase family of cysteine endoproteases.

We are currently using a variety of molecular and cellular methods to study the function of the profilaggrin N-terminus in keratinocyte differentiation. These include the yeast two-hybrid assay to examine protein-protein interactions in vivo, as well as gene expression in bacteria and cultured mammalian cells to study protein-protein interactions and protein function. Like profilaggrin, caspase-14 is also expressed in keratinizing epithelia late in keratinocyte differentiation. Recently, we determined the substrate specificity of recombinant caspase-14 expressed in E. coli. The precise function of caspase-14 in epidermis and other keratinizing epithelia is the subject of present investigation.

Other Expertise

Academic Experience:
Journal Reviewer
Journal of Biological Chemistry
Journal of Investigative Dermatology
Genomics
Americal Journal of Human Genetics

Postgraduate Award, Commonwealth Scholarship and Fellowship Plan (1981-86)


Keywords

COS Keywords:

Biochemistry, Biochemistry, Proteins, Biological Sciences, Cytoskeleton, Dentistry, Enzymes, Transgenic Animals.

Additional Terms:

Animal Models of Skin Disease, Cytoskeleton, Epidermis, Keratin, Keratinocyte, Profilaggrin, Protease, Proteolytic Enzyme, Transgenic Mouse.

Memberships

American Association for the Advancement of Science
American Society for Cell Biology
Society for Investigative Dermatology

Previous Positions

1994-2001, Research Assistant Professor, University of Washington, School of Dentistry, Oral Biology, Dermatology (Adjunct)
1992-1994, Research Associate, University of Washington
1989-1992, Senior Fellow, University of Washington
1987-1989, Post-doctoral Fellow, University of Adelaide

Funding Received

  • NIAMS (NIH): Protease-mediated events in epidermal differentiation, 2002 to 2007.
  • NIAMS (NIH): Profilaggrin in epidermal differentiation and function, 1998 to 2003.
  • Dermatology Foundation: Career Development Award, 1995 to 1998.

Publications

  • Cai S, Fatherazi S, Presland RB, Belton CM, Izutsu KT (Oct 2005) TRPC channel expression during calcium-induced differentiation of human gingival keratinocytes, Journal of Dermatological Science, 40 (1), 21-8
  • Wang H, Presland RB, Piepkorn M (Feb 2005) A search for CDKN2A/p16INK4a mutations in melanocytic nevi from patients with melanoma and spouse controls by use of laser-captured microdissection, Archives of Dermatology, 141 (2), 177-80
  • Presland RB, Fleckman P (2005) Tetracycline-regulated gene expression in epidermal keratinocytes, Methods in Molecular Biology (clifton, N.j.), 289, 273-86
  • Presland RB, Coulombe PA, Eckert RL, Mao-Qiang M, Feingold KR, Elias PM (Sep 2004) Barrier function in transgenic mice overexpressing K16, involucrin, and filaggrin in the suprabasal epidermis, The Journal of Investigative Dermatology, 123 (3), 603-6
  • Hitomi K, Presland RB, Nakayama T, Fleckman P, Dale BA, Maki M (August 2003) Analysis of epidermal-type transglutaminase (transglutaminase 3) in human stratified epithelia and cultured keratinocytes using monoclonal antibodies, Journal of Dermatological Science, 32 (2), 95-103
  • Pearton DJ, Dale BA, Presland RB (September 2002) Functional analysis of the profilaggrin N-terminal peptide: identification of domains that regulate nuclear and cytoplasmic distribution, Journal of Investigative Dermatology, 119 (3), 661-9
  • Chien A, Presland R, Kuechle M, Processing of native caspase-14 occurs at an atypical cleavage site in normal epidermal differentiation, Biochemical and Biophysical Research Communications, 296(4), 911, Aug 2002
  • Presland RB, Jurevic RJ (April 2002) Making sense of the epithelial barrier: what molecular biology and genetics tell us about the functions of oral mucosal and epidermal tissues, Journal of Dental Education, 66 (4), 564-74
  • Presland RB, Kuechle MK, Lewis SP, Fleckman P, Dale BA, Regulated expression of human filaggrin in keratinocytes results in cytoskeletal disruption, loss of cell-cell adhesion, and cell cycle arrest, Experimental Cell Research, 270(2), 199-213, November 2001
  • Presland RB, Tomic-Canic M, Lewis SP, Dale BA (November 2001) Regulation of human profilaggrin promoter activity in cultured epithelial cells by retinoic acid and glucocorticoids, Journal of Dermatological Science, 27 (3), 192-205
  • Kuechle MK, Predd HM, Fleckman P, Dale BA, Presland RB, Caspase-14, a keratinocyte specific caspase: mRNA splice variants and expression pattern in embryonic and adult mouse, Cell Death and Differentiation, 8(8), 868-70, August 2001
  • Pearton DJ, Nirunsuksiri W, Rehemtulla A, Lewis SP, Presland RB, Dale BA, Proprotein convertase expression and localization in epidermis: evidence for multiple roles and substrates, Experimental Dermatology, 10(3), 193-203, June 2001
  • Livingston RJ, Sybert VP, Smith LT, Dale BA, Presland RB, Stephens K (June 2001) Expression of a truncated keratin 5 may contribute to severe palmar--plantar hyperkeratosis in epidermolysis bullosa simplex patients, Journal of Investigative Dermatology, 116 (6), 970-4
  • Presland RB, Boggess D, Lewis SP, Hull C, Fleckman P, Sundberg JP (December 2000) Loss of normal profilaggrin and filaggrin in flaky tail (ft/ft) mice: an animal model for the filaggrin-deficient skin disease ichthyosis vulgaris, Journal of Investigative Dermatology, 115 (6), 1072-81
  • Kuechle MK, Presland RB, Lewis SP, Fleckman P, Dale BA, Inducible expression of filaggrin increases keratinocyte susceptibility to apoptotic cell death, Cell Death and Differentiation, 7(6), 566-73, June 2000
  • Presland RB, Dale BA, Epithelial structural proteins of the skin and oral cavity: function in health and disease, Critical Reviews in Oral Biology and Medicine, 11(4), 383-408, 2000
  • Kuechle MK, Thulin CD, Presland RB, Dale BA (June 1999) Profilaggrin requires both linker and filaggrin peptide sequences to form granules: implications for profilaggrin processing in vivo, Journal of Investigative Dermatology, 112 (6), 843-52
  • Ishida-Yamamoto A, Takahashi H, Presland RB, Dale BA, Iizuka H, Translocation of profilaggrin N-terminal domain into keratinocyte nuclei with fragmented DNA in normal human skin and loricrin keratoderma, Laboratory Investigation, 78(10), 1245-53, October 1998
  • Presland R B, Kimball J R, Kautsky M B, Lewis S P, Lo C Y, Dale B A (February 1997) Evidence for specific proteolytic cleavage of the N-terminal domain of human profilaggrin during epidermal differentiation, Journal of Investigative Dermatology, 108 (2), 170-8
  • Dale B A, Presland R B, Lewis S P, Underwood R A, Fleckman P (February 1997) Transient expression of epidermal filaggrin in cultured cells causes collapse of intermediate filament networks with alteration of cell shape and nuclear integrity, Journal of Investigative Dermatology, 108 (2), 179-87
  • Presland R B, Bassuk J A, Kimball J R, Dale B A (February 1995) Characterization of two distinct calcium-binding sites in the amino-terminus of human profilaggrin, Journal of Investigative Dermatology, 104 (2), 218-23
  • Nirunsuksiri W, Presland R B, Brumbaugh S G, Dale B A, Fleckman P (13 Jan 1995) Decreased profilaggrin expression in ichthyosis vulgaris is a result of selectively impaired posttranscriptional control, Journal of Biological Chemistry, 270 (2), 871-6
  • Presland RB, Haydock PV, Fleckman P, Nirunsuksiri W, Dale BA (Nov 1992) Characterization of the human epidermal profilaggrin gene. Genomic organization and identification of an S-100-like calcium binding domain at the amino terminus, The Journal of Biological Chemistry, 267 (33), 23772-81
  • Dale BA, Jones AH, Presland R, Adler DA, Disteche CM (Apr-Jun 1992) Chromosomal localization of the retinol binding protein gene and its elimination as a candidate gene for the repeated epilation (Er) mutation in mice, Journal of Craniofacial Genetics and Developmental Biology, 12 (2), 76-81
  • Fietz MJ, Presland RB, Rogers GE (February 1990) The cDNA-deduced amino acid sequence for trichohyalin, a differentiation marker in the hair follicle, contains a 23 amino acid repeat, Journal of Cell Biology, 110 (2), 427-36
  • Presland RB, Whitbread LA, Rogers GE (Oct 1989) Avian keratin genes. II. Chromosomal arrangement and close linkage of three gene families, Journal of Molecular Biology, 209 (4), 561-76
  • Presland RB, Gregg K, Molloy PL, Morris CP, Crocker LA, Rogers GE (Oct 1989) Avian keratin genes. I. A molecular analysis of the structure and expression of a group of feather keratin genes, Journal of Molecular Biology, 209 (4), 549-59

Profile Details

Last Verified: 9/11/2006

COS Expertise ID #414263
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