Prof. David Rhodes

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Purdue University
Agriculture
Horticulture & Landscape Architecture
ProfessorAppointed: 1991

Mailing Address

Purdue University
Department of Horticulture and Landscape Architecture
Horticulture Building
625 Agriculture Mall Drive
West Lafayette, Indiana 47907-2010
United States

Contact Information

Phone: (765) 494-1312
Fax: (765) 494-0391
drhodes@purdue.edu
http://www.hort.purdue.edu/rhodcv/index.htm

Qualifications

Ph.D., University of Manchester, Plant Physiology, 1976.
B.Sc., University of Manchester, Botany, 1973.

Expertise and Research Interests

Research interests include plant metabolic adaptations to environmental stress, with particular emphasis on the biochemical genetics of quaternary ammonium and tertiary sulfonium compound synthesis. Quaternary ammonium and tertiary sulfonium compounds are thought to function as compatible cytoplasmic osmotic solutes, and may play a role in plant adaptation to salinity, drought, high temperature and cold temperature stresses. Genes encoding enzymes of quaternary ammonium and tertiary sulfonium compound synthesis are rational candidates for metabolic engineering of plant stress tolerance. More recently, research is focusing on metabolic flux analysis in pathways of biosynthesis of compounds contributing to floral scent.

Other Expertise

Computer simulation of plant metabolism

Future Research

Future research will primarily focus on modeling of plant metabolism and interpretation of transient and dynamic stable isotope fluxes in plant metabolic systems

Industrial Relevance

Metabolic engineering of plants will rely increasingly on the use of integrative mathematical models of plant metabolism

Keywords

COS Keywords:

Africa, Agriculture, Agriculture Education, Agronomy, Amino Acids, Analytical Chemistry, Aquacultural Engineering, Australia, Biochemistry, Biochemistry, Carbohydrates, Biochemistry, Lipids, Biochemistry, Nucleic Acids, Biochemistry, Proteins, Bioengineering, Biological Sciences, Biotechnology, Botany, British Isles, Cell Biology, Crop Science, Deserts and Arid Zones, Developmental Biology, Enzymology, Europe, Fertilizers, Genetics, Grains, Herbicides, Horticulture, International, International Agriculture, Irrigation, Metabolism, Molecular Biology, North America, Oceania (Including Australia), Pesticides, Photosynthesis, Plant Diseases Or Pathology, Plant Genetics, Plant Nutrition, Plant Or Soil Nutrient Bioavailability, Plant Physiology Or Morphology, Plant Sciences, Plant Virology, Salinity, Seed Production, United Kingdom, USA, Midwest, Weed Sciences.

Additional Terms:

Amino Acids, Betaines, Biochemistry, Dimethylsulfoniopropionate, Drought, Floral Scent, Genetics, Heat, Maize, Metabolism, Modeling, Osmotic Stress, Salinity.

Memberships

American Association for the Advancement of Science

Previous Positions

1976-1978, University of East Anglia, School of Biological Sciences
1980-1985, Zoecon Corporation, Palo Alto, CA, Senior Research Biologist
1979-1980, Michigan State University-Department of Energy Plant Research Lab., Michigan State University, Postdoctoral Research Associate
1985-1991, Dept. of Horticulture, Purdue University, Associate Professor

Funding Received

  • National Science Foundation - Metabolic Biochemistry: Regulation of monoterpene emission in snapdragon flowers (N. Dudareva and D. Rhodes), $435,000, Jan 1, 2003 to Dec 31, 2005.
  • United States Department of Agriculture (USDA) - Plant Biochemistry: Benzenoid network in petunia flowers (N. Dudareva and D. Rhodes), $195,000, Aug 1, 2003 to Jul 31, 2005.

Publications

  • Dudareva N, Andersson S, Orlova I, Gatto N, Reichelt M, Rhodes D, Boland W, Gershenzon J (Jan 2005) The nonmevalonate pathway supports both monoterpene and sesquiterpene formation in snapdragon flowers., Proceedings of the National Academy of Sciences of the United States of America., 102 (3), 933-8 Abstract
  • Boatright J, Negre F, Chen X, Kish CM, Wood B, Peel G, Orlova I, Gang D, Rhodes D, Dudareva N (Aug 2004) Understanding in vivo benzenoid metabolism in petunia petal tissue., Plant Physiology, 135 (4), 1993-2011 Abstract
  • Inan G, Zhang Q, Li P, Wang Z, Cao Z, Zhang H, Zhang C, Quist TM, Goodwin SM, Zhu J, Shi H, Damsz B, Charbaji T, Gong Q, Ma S, Fredricksen M, Galbraith DW, Jenks MA, Rhodes D, Hasegawa PM, Bohnert HJ, Joly RJ, Bressan RA, Zhu JK (Jul 2004) Salt cress. A halophyte and cryophyte Arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles., Plant Physiology, 135 (3), 1718-37 Abstract
  • Goyer A, Johnson TL, Olsen LJ, Collakova E, Shachar-Hill Y, Rhodes D, Hanson AD, Characterization and metabolic function of a peroxisomal sarcosine and pipecolate oxidase from Arabidopsis, Journal of Bioligical Chemistry, 279(17), 16947-53, April 2004 Abstract
  • Rontein D, Rhodes D, Hanson AD (Nov 2003) Evidence from engineering that decarboxylation of free serine is the major source of ethanolamine moieties in plants., Plant & Cell Physiology, 44 (11), 1185-91 Abstract
  • Kocsis MG, Ranocha P, Gage DA, Simon ES, Rhodes D, Peel GJ, Mellema S, Saito K, Awazuhara M, Li C, Meeley RB, Tarczynski MC, Wagner C, Hanson AD (Apr 2003) Insertional inactivation of the methionine s-methyltransferase gene eliminates the s-methylmethionine cycle and increases the methylation ratio., Plant Physiology, 131 (4), 1808-15 Abstract
  • Mickelbart MV, Peel G, Joly RJ, Rhodes D, Ejeta G, Goldsbrough PB, Development and characterization of near-isogenic lines of sorghum segregating for glycinebetaine accumulation, Physiologia Plantarum, 118, 253-261, 2003
  • Yang W-J, Rich PJ, Axtell JD, Wood KV, Bonham CC, Ejeta G, Mickelbart MV, Rhodes D, Genotypic variation for glycinebetaine in sorghum, Crop Science, 43, 162-169, 2003
  • Wood KV, Bonham CC, Miles D, Rothwell AP, Peel G, Wood BC, Rhodes D, Characterization of betaines using electrospray MS/MS, Phytochemistry, 59(7), 759-65, April 2002 Abstract
  • Rhodes D, Nadolska-Orczyk A, Rich PJ, Salinity, osmolytes and compatible solutes, In (A. Läuchli, U. Lüttge, eds.) 'Salinity: Environment - Plants - Molecules', Kluwer Academic, Boston, N.Y., 181-204, 2002
  • Morgan JA, Rhodes D, Mathematical modeling of plant metabolic pathways, Metabolic Engineering, 4(1), 80-9, January 2002 Abstract
  • Fletcher SA, Rhodes D, Csonka LN, Analysis of the effects of osmoprotectants on the high osmolality-dependent induction of increased thermotolerance in Salmonella typhimurium, Food Microbiology, 18, 345-354, 2001
  • McNeil SD, Rhodes D, Russell BL, Nuccio ML, Shachar-Hill Y, Hanson AD, Metabolic modeling identifies key constraints on an engineered glycine betaine synthesis pathway in tobacco, Plant Physiology, 124(1), 153-62, September 2000 Abstract
  • Jenks MA, Rich PJ, Rhodes D, Ashwort EN, Axtell JD, Din CK, Leaf sheath cuticular waxes on bloomless and sparse-bloom mutants of Sorghum bicolor, Phytochemistry, 54(6), 577-84, July 2000 Abstract
  • Ranocha P, Bourgis F, Ziemak MJ, Rhodes D, Gage DA, Hanson AD, Characterization and functional expression of cDNAs encoding methionine-sensitive and -insensitive homocysteine S-methyltransferases from Arabidopsis, Journal of Biological Chemistry, 275(21), 15962-8, 2000 Abstract
  • McNeil SD, Nuccio ML, Rhodes D, Shachar-Hill Y, Hanson AD, Radiotracer and computer modeling evidence that phospho-base methylation is the main route of choline synthesis in tobacco, Plant Physiology, 123(1), 371-80, May 2000 Abstract
  • Joly RJ, Jones ML, Verlinden S, Rhodes D, Woodson WR, Learning in an inquiry-driven plant physiology laboratory, Journal of Natural Resources and Life Sciences Education, 29, 31-35, 2000
  • Nuccio ML, Rhodes D, McNeil SD, Hanson AD, Metabolic engineering of plants for osmotic stress resistance, Current Opinion in Plant Biology, 2(2), 128-34, April 1999 Abstract
  • Kocsis MG, Nolte KD, Rhodes D, Shen TL, Gage DA, Hanson AD, Dimethylsulfoniopropionate biosynthesis in Spartina alterniflora1. Evidence that S-methylmethionine and dimethylsulfoniopropylamine are intermediates, Plant Physiology, 117(1), 273-81, May 1998 Abstract
  • Summers PS, Nolte, KD, Cooper AJL, Borgeas H, Leustek T, Rhodes D, Hanson AD, Identification and stereospecificity of the first three enzymes of 3-dimethylsulfoniopropionate biosynthesis in a chlorophyte alga, Plant Physiology, 116, 369-378, 1998
  • Rhodes D, Verslues PE, Sharp RE, Role of amino acids in abiotic stress resistance, In (B.K. Singh, ed.) 'Plant Amino Acids: Biochemistry and Biotechnology', Marcel Dekker, Inc., New York, 319-356, 1998
  • Canovas D, Vargas C, Iglesias-Guerra F, Csonka LN, Rhodes D, Ventosa A, Nieto JJ, Isolation and characterization of salt-sensitive mutants of the moderate halophile Halomonas elongata and cloning of the ectoine synthesis genes, Journal of Biological Chemistry, 272(41), 25794-801, 1997 Abstract
  • Gage DA, Rhodes D, Nolte KD, Hicks WA, Leustek T, Cooper AJ, Hanson AD, A new route for synthesis of dimethylsulphoniopropionate in marine algae, Nature, 387(6636), 891-4, 1997 Abstract
  • Rhodes D, Gage DA, Cooper AJL, Hanson AD, S-Methylmethionine conversion to dimethylsulfoniopropionate: evidence for an unusual transamination reaction, Plant Physiology, 115, 1541-1548, 1997
  • Wood AJ, Saneoka H, Rhodes D, Joly RJ, Goldsbrough PB, Betaine aldehyde dehydrogenase in sorghum, Plant Physiology, 110(4), 1301-8, April 1996 Abstract
  • Yang G, Rhodes D, Joly RJ, Effects of high temperature on membrane stability and chlorophyll fluorescence in glycinebetaine-deficient and glycinebetaine-containing maize lines, Aust. J. Plant Physiol., 23, 437-443, 1996
  • Sheets EB, Rhodes D, Determination of DMSP and other onium compounds in Tetraselmis subcordiformis by plasma desorption mass spectrometry, In (R. Kiene, P. Visscher, G. Kirst, M. Keller, eds.) 'Biological and Environmental Chemistry of Dimethylsulfoniopropionate and Related Sulfonium Compounds.' Plenum Press, New York, 55-63, 1996
  • Liu D, Rhodes D, Paino D'Urzo M, Xu Y, Narasimhan ML, Hasegawa PM, Bressan RA, Abad L, In vivo and in vitro activity of truncated osmotin that is secreted into the extracellular matrix, Plant Science, 121, 123-131, 1996
  • Yang WJ, Nadolska-Orczyk A, Wood KV, Hahn DT, Rich PJ, Wood AJ, Saneoka H, Premachandra GS, Bonham CC, Rhodes JC, Joly RJ, Samaras Y, Goldsbrough PB, Rhodes D, Near-isogenic lines of maize differing for glycinebetaine, Plant Physiology, 107(2), 621-30, February 1995
  • Bonham CC, Wood KV, Yang W-J, Nadolska-Orczyk A, Samaras Y, Gage DA, Poupart J, Burnet M, Hanson AD, Rhodes D, Identification of quaternary ammonium and tertiary sulfonium compounds by plasma desorption mass spectrometry, Journal of Mass Spectrometry, 30, 1187-1194, 1995
  • Saneoka H, Nagasaka C, Hahn DT, Yang W-J, Premachandra GS, Joly RJ, Rhodes D, Salt tolerance of glycinebetaine-deficient and -containing maize lines, Plant Physiology, 107, 631-638, 1995
  • Premachandra GS, Hahn DT, Rhodes D, Joly RJ, Leaf water relations and solute accumulation in two grain sorghum lines exhibiting contrasting drought tolerance, Journal of Experimental Botany, 46, 1833-1841, 1995
  • Samaras Y, Bressan RA, Csonka LN, García-Ríos MG, Paino D'Urzo M, Rhodes D, Proline accumulation during drought and salinity, In (N. Smirnoff, ed.) 'Environment and Plant Metabolism', Bios Scientific Publishers, Oxford, 161-187, 1995
  • Grote EM, Ejeta G, Rhodes D, Inheritance of glycinebetaine deficiency in sorghum, Crop Science, 34, 1217-1220, 1994
  • Hasegawa PM, Bressan RA, Nelson DE, Samaras Y, Rhodes D, Tissue culture in the improvement of salt tolerance in plants, In (A.R. Yeo, T.J. Flowers, eds.) 'Soil Mineral Stresses. Approaches to Crop Improvement. Monographs on Theoretical and Applied Genetics', Vol 21, Springer-Verlag, Berlin, 83-125, 1994
  • Nussbaum S, von Ballmoos P, Gfeller H, Schlunegger UP, Fuhrer J, Rhodes D, Brunold C, Incorporation of 15NO2-nitrogen into free amino acids by Norway spruce Picea abies (L.) Karst, Oecologia, 94, 408-414, 1993
  • Rhodes D, Samaras Y, Genetic control of osmoregulation in plants, In (K. Strange, ed.) 'Cellular and Molecular Physiology of Cell Volume Regulation', CRC Press, Boca Raton, 347-361, 1993
  • Rhodes D, Hanson AD, Quaternary ammonium and tertiary sulfonium compounds in higher plants, Annu. Rev. Plant Physiol. Plant Mol. Biol., 44, 357-384, 1993
  • Monyo ES, Ejeta G, Rhodes D, Genotypic variation for glycinebetaine in sorghum and its relationship to agronomic and morphological traits, Maydica, 37, 283-286, 1992
  • Rhodes D, Ju GC, Yang W-J, Samaras Y, Plant metabolism and heterosis, Plant Breeding Reviews, 10, 53-91, 1992
  • Saranga Y, Rhodes D, Janick J, Changes in amino acid composition associated with the tolerance to partial desiccation of celery somatic embryos, Journal of the American Society for Horticultural Science, 117, 337-341, 1992
  • LaRosa PC, Rhodes D, Rhodes JC, Bressan RA, Csonka LN, Elevated accumulation of proline in NaCl-adapted tobacco cells is not due to altered delta1-pyrroline-5-carboxylate reductase, Plant Physiology, 96, 245-250, 1991
  • Lerma C, Rich PJ, Ju GC, Yang W-J, Hanson AD, Rhodes D, Betaine deficiency in maize: Complementation tests and metabolic basis, Plant Physiology, 95, 1113-1119, 1991
  • Wood KV, Stringham KJ, Smith DL, Volenec JJ, Hendershot KL, Jackson KA, Rich PJ, Yang W-J, Rhodes D, Betaines of alfalfa: Characterization by fast atom bombardment and desorption chemical ionization mass spectrometry, Plant Physiology, 96, 892-897, 1991
  • Magalhães JR, Ju GC, Rich PJ, Rhodes D, Kinetics of 15NH4+ assimilation in Zea mays : Preliminary studies with a glutamate dehydrogenase (GDH1) null mutant, Plant Physiology, 94, 647-656, 1990
  • Mayer RR, Cherry JH, Rhodes D, . Effects of heat shock on amino acid metabolism of cowpea cells, Plant Physiology, 94, 796-810, 1990
  • Avery M, Rhodes D, Growth characteristics and total N content of a Leucaena/Sorghum agroforestry system, Plant and Soil, 127, 259-267, 1990
  • Rhodes D, Fast atom bombardment mass spectrometry, In (H.-F. Linskens, J.F. Jackson, eds.) 'Modern Methods of Plant Analysis. New Series, Vol. 11. Physical Methods in Plant Science', Springer-Verlag, Berlin, 95-123, 1990
  • Brunk DG, Rich PJ, Rhodes D, Genotypic variation for glycinebetaine among public inbreds of maize, Plant Physiology, 91, 1122-1125, 1989
  • Rhodes D, Rich PJ, Brunk DG, Ju GC, Rhodes JC, Pauly MH, Hansen LA, Development of two isogenic sweet corn hybrids differing for glycinebetaine content, Plant Physiology, 91, 1112-1121, 1989
  • Rhodes D, Brunk DG, Magalhaes JR, Assimilation of ammonia by glutamate dehydrogenase?, In (J.E. Poulton, J.T. Romeo, E.E. Conn, eds.) 'Plant Nitrogen Metabolism', Recent Advances in Phytochemistry, Vol. 23. Plenum Press, New York, 191-226, 1989
  • Weretilnyk EA, Bednarek S, McCue KF, Rhodes D, Hanson AD, Comparative biochemical and immunological studies of the glycine betaine synthesis pathway in diverse families of dicotyledons, Planta, 178, 342-352, 1989
  • Rhodes D, Rich PJ, Brunk DG, Amino acid metabolism of Lemna minor L. IV. 15N-Labeling kinetics of the amide and amino groups of glutamine and asparagine, Plant Physiology, 89, 1161-1171, 1989
  • Brouquisse R, Weigel P, Rhodes D, Yocum C, Hanson AD, Evidence for a ferredoxin-dependent choline monooxygenase from spinach chloroplast stroma, Plant Physiology, 90, 322-329, 1989
  • Rhodes D, Handa S, Amino acid metabolism in relation to osmotic adjustment, In (J.H. Cherry, ed.) 'Environmental Stress in Plants. Biochemical and Physiological Mechanisms', NATO ASI Series G: Ecological Sciences, Vol 19, Springer-Verlag, Berlin, 41-62, 1989
  • Lerma C, Hanson AD, Rhodes D, Oxygen-18 and deuterium labeling studies of choline oxidation by spinach and sugar beet, Plant Physiology, 88, 695-702, 1988
  • Rhodes D, Rich PJ, Preliminary genetic studies of the phenotype of betaine deficiency in Zea mays L, Plant Physiology, 88, 102-108, 1988
  • Brunk DG, Rhodes D, Amino acid metabolism of Lemna minor L. III. Responses to aminooxyacetate, Plant Physiology, 87, 447-453, 1988
  • LaRosa PC, Hasegawa PM, Rhodes D, Clithero JM, Watad A-EA, Bressan RA, Abscisic acid stimulated osmotic adjustment and its involvement in adaptation of tobacco cells to NaCl, Plant Physiology, 85, 174-181, 1987
  • Binzel ML, Hasegawa PM, Rhodes D, Handa S, Handa AK, Bressan RA, Solute accumulation in tobacco cells adapted to NaCl, Plant Physiology, 84, 1408-1415, 1987
  • Rhodes D, Metabolic responses to stress, In (D.D. Davies, ed.) 'The Biochemistry of Plants', Vol 12, Academic Press, New York, 201-241, 1987
  • Rhodes D, Rich PJ, Myers AC, Reuter CC, Jamieson GC, Determination of betaines by fast atom bombardment mass spectrometry: Identification of glycine betaine deficient genotypes of Zea mays, Plant Physiology, 84, 781-788, 1987
  • Rhodes D, Hogan AL, Deal L, Jamieson GC, Haworth P, Amino acid metabolism of Lemna minor L. II. Responses to chlorsulfuron, Plant Physiology, 84, 775-780, 1987
  • Rhodes D, Deal L, Haworth P, Jamieson GC, Reuter CC, Ericson MC, Amino acid metabolism of Lemna minor L. I. Responses to methionine sulfoximine, Plant Physiology, 82, 1056-1062, 1986
  • Rhodes D, Handa S, Bressan RA, Metabolic changes associated with adaptation of plant cells to water stress, Plant Physiology, 82, 890-903, 1986
  • Yamaya T, Oaks A, Rhodes D, Matsumoto H, Synthesis of [15N]glutamate from [15N]H4+ and [15N]glycine by mitochondria isolated from pea and corn shoots, Plant Physiology, 81, 754-757, 1986
  • Hanson AD, May AM, Grumet R, Bode J, Jamieson GC, Rhodes D, Betaine synthesis in chenopods: Localization in chloroplasts, Proceedings of the National Academy of Sciences (USA), 82, 3678-3682, 1985
  • Rosenthal GA, Rhodes D, L-Canavanine transport and utilization in developing jack bean, Canavalia ensiformis (L.) DC. [Leguminosae], Plant Physiology, 76, 541-544, 1984
  • Hanson AD, Rhodes D, 14C tracer evidence for synthesis of choline and betaine via phosphoryl base intermediates in salinized sugar beet leaves, Plant Physiology, 71, 692-700, 1983
  • Rhodes D, Myers AC, Jamieson G, Gas chromatography-mass spectrometry of N-heptafluorobutyryl isobutyl esters of amino acids in the analysis of the kinetics of [15N]H4+ assimilation in Lemna minor L, Plant Physiology, 68, 1197-1205, 1981
  • Hitz WD, Rhodes D, Hanson AD, Radiotracer evidence implicating phosphoryl and phosphatidyl bases as intermediates in betaine synthesis by water-stressed barley leaves, Plant Physiology, 68, 814-822, 1981
  • Rhodes D, Sims AP, Folkes BF, Pathway of ammonia assimilation in illuminated Lemna minor, Phytochemistry, 19, 357-365, 1980
  • Rhodes D, Sims AP, Stewart GR, Glutamine synthetase and the control of nitrogen assimilation in Lemna minor L, In (E.J. Hewitt, C.V. Cutting, eds.)'Nitrogen Assimilation of Plants', Academic Press, London, 59-78, 1979
  • Stewart GR, Rhodes D, Nitrogen metabolism of halophytes III. Enzymes of ammonia assimilation, New Phytologist, 80, 307-316, 1978
  • Stewart GR, Rhodes D, A comparison of the characteristics of glutamine synthetase and glutamate dehydrogenase from Lemna minor L, New Phytologist, 79, 257-268, 1977
  • Stewart GR, Rhodes D, . Control of enzyme levels in the regulation of nitrogen assimilation, In (H. Smith, ed.), 'Regulation of Enzyme Synthesis and Activity in Higher Plants', Academic Press, London, 1-22, 1977
  • Stewart GR, Rhodes D, Evidence for the assimilation of ammonia via the glutamine pathway in nitrate-grown Lemna minor L, FEBS Letters, 64, 296-299, 1976
  • Rhodes D, Rendon GA, Stewart GR, The regulation of ammonia assimilating enzymes in Lemna minor, Planta, 129, 203-210, 1976
  • Rhodes D, Rendon GA, Stewart GR, The control of glutamine synthetase level in Lemna minor L, Planta, 125, 201-211, 1975
  • Rhodes D, Stewart GR, A procedure for the in vivo determination of enzyme activity in higher plant tissue, Planta, 118, 133-144, 1974

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