Prof. Rola Barhoumi

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Texas A&M University
College of Veterinary Medicine
Veterinary Integrative BioSciences
Research ProfessorAppointed: 1991
Texas A&M University
College of Veterinary Medicine
Veterinary Anatomy and Public Health
Research Scientist

Mailing Address

Texas A&M University
College of Veterinary Medicine
Veterinary Integrative BioSciences
College Station, Texas 77843-4458
United States

Contact Information

Phone: (979) 458-1149
Fax: (979) 847-8981
rmouneimne@cvm.tamu.edu

Qualifications

Ph.D., Universite Claude Bernard - Lyon I - France, Material Science, 1984.
M.Sc., Universite Claude Bernard - Lyon I - France, Material Science, 1983.
B.Sc., Beirut Arab University, Electrical Engineering, 1980.

Expertise and Research Interests

My current research involves the use of fluorescent biosensors and biomarkers for evaluating the function of living cells and tissues. I developed methods to monitor a variety of endpoints in cells using commercially available fluorescent molecules including: 1-Detection and analysis of intracellular Calcium content, calcium fluxes, intrinsic and induced calcium oscillations. 2-Analysis of calcium changes induced by environmental factors. 2-Analysis of mitochondrial membrane potential, description of oscillations in mitochondrial membrane potential, and detection of mitochondrial permeability transition following toxic insults. 3-Detection and quantitative assessment of gap junction mediated intercellular communication (diffusion of fluorescent probes between cells) and metabolic cooperation (cell-cell sharing of specific molecules such as glutathione) 4-Determination of chronology of events involved in toxicity mechanisms 5-Quantitative analysis and mathematical modeling of cellular signaling.

Keywords

COS Keywords:

Automation, Image Processing, Microscopy, Oscillations, Public Health, Toxicology, Veterinary Medicine.

Additional Terms:

Automation, Cellular Signaling, Fluorescence Microscopy, IMAQ VISION Builder, Image Analysis, LabView, Oscillations, Toxicity Mechanisms.

Languages

(Reading, Writing, Speaking)

Arabic: (Fluent, Fluent, Fluent)
English: (Fluent, Fluent, Fluent)
French: (Fluent, Fluent, Fluent)

Publications

  • Kim W, Fan YY, Barhoumi R, Smith R, McMurray DN, Chapkin RS (Nov 2008) n-3 polyunsaturated fatty acids suppress the localization and activation of signaling proteins at the immunological synapse in murine CD4+ T cells by affecting lipid raft formation., Journal of immunology (Baltimore, Md. : 1950), 181 (9), 6236-43 Abstract
  • Ly LH, Barhoumi R, Cho SH, Franzblau SG, McMurray DN (Sep 2008) Vaccination with Bacille-Calmette Guérin promotes mycobacterial control in guinea pig macrophages infected in vivo., The Journal of infectious diseases, 198 (5), 768-71 Abstract
  • Wu F, Khan S, Wu Q, Barhoumi R, Burghardt R, Safe S (May 2008) Ligand structure-dependent activation of estrogen receptor alpha/Sp by estrogens and xenoestrogens., The Journal of steroid biochemistry and molecular biology, 110 (1-2), 104-15 Abstract
  • Kolar SS, Barhoumi R, Callaway ES, Fan YY, Wang N, Lupton JR, Chapkin RS (Nov 2007) Synergy between docosahexaenoic acid and butyrate elicits p53-independent apoptosis via mitochondrial Ca(2+) accumulation in colonocytes., American journal of physiology. Gastrointestinal and liver physiology, 293 (5), G935-43 Abstract
  • Kolar SS, Barhoumi R, Lupton JR, Chapkin RS (Jun 2007) Docosahexaenoic acid and butyrate synergistically induce colonocyte apoptosis by enhancing mitochondrial Ca2+ accumulation., Cancer research, 67 (11), 5561-8 Abstract
  • Barhoumi R, Burghardt RC, Qian Y, Tiffany-Castiglioni E (May 2007) Effects of propofol on intracellular Ca2+ homeostasis in human astrocytoma cells., Brain research, 1145, 11-8 Abstract
  • Qian Y, Venkatraj J, Barhoumi R, Pal R, Datta A, Wild JR, Tiffany-Castiglioni E (Mar 2007) Comparative non-cholinergic neurotoxic effects of paraoxon and diisopropyl fluorophosphate (DFP) on human neuroblastoma and astrocytoma cell lines., Toxicology and applied pharmacology, 219 (2-3), 162-71 Abstract
  • Tjalkens RB, Zoran MJ, Mohl B, Barhoumi R (Oct 2006) Manganese suppresses ATP-dependent intercellular calcium waves in astrocyte networks through alteration of mitochondrial and endoplasmic reticulum calcium dynamics., Brain research, 1113 (1), 210-9 Abstract
  • Khan S, Barhoumi R, Burghardt R, Liu S, Kim K, Safe S (Sep 2006) Molecular mechanism of inhibitory aryl hydrocarbon receptor-estrogen receptor/Sp1 cross talk in breast cancer cells., Molecular endocrinology (Baltimore, Md.), 20 (9), 2199-214 Abstract
  • Barhoumi R, Awooda I, Mouneimne Y, Safe S, Burghardt RC (Aug 2006) Effects of benzo-a-pyrene on oxytocin-induced Ca2+ oscillations in myometrial cells., Toxicology letters, 165 (2), 133-41 Abstract
  • Vanderlaag K, Samudio I, Burghardt R, Barhoumi R, Safe S (May 2006) Inhibition of breast cancer cell growth and induction of cell death by 1,1-bis(3'-indolyl)methane (DIM) and 5,5'-dibromoDIM., Cancer letters, 236 (2), 198-212 Abstract
  • Seo J, Barhoumi R, Johnson AE, Lupton JR, Chapkin RS (Apr 2006) Docosahexaenoic acid selectively inhibits plasma membrane targeting of lipidated proteins., The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20 (6), 770-2 Abstract
  • Abdelrahim M, Ariazi E, Kim K, Khan S, Barhoumi R, Burghardt R, Liu S, Hill D, Finnell R, Wlodarczyk B, Jordan VC, Safe S (Feb 2006) 3-Methylcholanthrene and other aryl hydrocarbon receptor agonists directly activate estrogen receptor alpha., Cancer research, 66 (4), 2459-67 Abstract
  • Forough R, Weylie B, Collins C, Parker JL, Zhu J, Barhoumi R, Watson DK (2006) Transcription factor Ets-1 regulates fibroblast growth factor-1-mediated angiogenesis in vivo: role of Ets-1 in the regulation of the PI3K/AKT/MMP-1 pathway., Journal of vascular research, 43 (4), 327-37 Abstract
  • Ng Y, Barhoumi R, Tjalkens RB, Fan YY, Kolar S, Wang N, Lupton JR, Chapkin RS (Nov 2005) The role of docosahexaenoic acid in mediating mitochondrial membrane lipid oxidation and apoptosis in colonocytes., Carcinogenesis, 26 (11), 1914-21 Abstract
  • Ottinger S, Barhoumi R, McKenzie KS, McDonald T, Burghardt R, Huebner HJ, Phillips TD (Aug 2005) FIA/MS analysis of temporally ozonated benzo[a]pyrene and pyrene and their reaction products: inhibition of gap junction-mediated intercellular communication., Chemosphere, 60 (8), 1025-33 Abstract
  • Partridge CR, Williams ES, Barhoumi R, Tadesse MG, Johnson CD, Lu KP, Meininger GA, Wilson E, Ramos KS (Jun 2005) Novel genomic targets in oxidant-induced vascular injury., Journal of molecular and cellular cardiology, 38 (6), 983-96 Abstract
  • Kim K, Barhoumi R, Burghardt R, Safe S (Apr 2005) Analysis of estrogen receptor alpha-Sp1 interactions in breast cancer cells by fluorescence resonance energy transfer., Molecular endocrinology (Baltimore, Md.), 19 (4), 843-54 Abstract
  • Pietrantonio PV, Jagge C, Taneja-Bageshwar S, Nachman RJ, Barhoumi R (Jan 2005) The mosquito Aedes aegypti (L.) leucokinin receptor is a multiligand receptor for the three Aedes kinins., Insect molecular biology, 14 (1), 55-67 Abstract
  • Sanders LM, Henderson CE, Hong MY, Barhoumi R, Burghardt RC, Wang N, Spinka CM, Carroll RJ, Turner ND, Chapkin RS, Lupton JR (Dec 2004) An increase in reactive oxygen species by dietary fish oil coupled with the attenuation of antioxidant defenses by dietary pectin enhances rat colonocyte apoptosis., The Journal of nutrition, 134 (12), 3233-8 Abstract
  • Fan YY, Ly LH, Barhoumi R, McMurray DN, Chapkin RS (Nov 2004) Dietary docosahexaenoic acid suppresses T cell protein kinase C theta lipid raft recruitment and IL-2 production., Journal of immunology (Baltimore, Md. : 1950), 173 (10), 6151-60 Abstract
  • Sanders LM, Henderson CE, Hong MY, Barhoumi R, Burghardt RC, Carroll RJ, Turner ND, Chapkin RS, Lupton JR (May 2004) Pro-oxidant environment of the colon compared to the small intestine may contribute to greater cancer susceptibility., Cancer letters, 208 (2), 155-61 Abstract
  • Barhoumi R, Faske J, Liu X, Tjalkens RB (Mar 2004) Manganese potentiates lipopolysaccharide-induced expression of NOS2 in C6 glioma cells through mitochondrial-dependent activation of nuclear factor kappaB., Brain research. Molecular brain research, 122 (2), 167-79 Abstract
  • Cizmas L, Barhoumi R, Burghardt RC, Reeves WR, He L, McDonald TJ, Donnelly KC (Jul 2003) A comparison of two methods for fractionating complex mixtures in preparation for toxicity analysis., Journal of toxicology and environmental health. Part A, 66 (14), 1351-70 Abstract
  • Holmes SP, Barhoumi R, Nachman RJ, Pietrantonio PV (Feb 2003) Functional analysis of a G protein-coupled receptor from the southern cattle tick Boophilus microplus (Acari: Ixodidae) identifies it as the first arthropod myokinin receptor., Insect molecular biology, 12 (1), 27-38 Abstract
  • Hong MS, Hong SJ, Barhoumi R, Burghardt RC, Donnelly KC, Wild JR, Venkatraj V, Tiffany-Castiglioni E (Jan 2003) Neurotoxicity induced in differentiated SK-N-SH-SY5Y human neuroblastoma cells by organophosphorus compounds., Toxicology and applied pharmacology, 186 (2), 110-8 Abstract
  • Hong MY, Chapkin RS, Barhoumi R, Burghardt RC, Turner ND, Henderson CE, Sanders LM, Fan YY, Davidson LA, Murphy ME, Spinka CM, Carroll RJ, Lupton JR (Nov 2002) Fish oil increases mitochondrial phospholipid unsaturation, upregulating reactive oxygen species and apoptosis in rat colonocytes., Carcinogenesis, 23 (11), 1919-25 Abstract
  • Barhoumi R, Mouneimne Y, Awooda I, Safe SH, Donnelly KC, Burghardt RC (Aug 2002) Characterization of calcium oscillations in normal and benzo[a]pyrene-treated clone 9 cells., Toxicological sciences : an official journal of the Society of Toxicology, 68 (2), 444-50 Abstract
  • Botos J, Barhoumi R, Burghardt R, Kochevar DT (Apr 2002) Rb localization and phosphorylation kinetics correlate with the cellular phenotype of cultured breast adenocarcinoma cells., In vitro cellular & developmental biology. Animal, 38 (4), 235-41 Abstract
  • Mealey KL, Barhoumi R, Burghardt RC, Safe S, Kochevar DT (Mar 2002) Doxycycline induces expression of P glycoprotein in MCF-7 breast carcinoma cells., Antimicrobial agents and chemotherapy, 46 (3), 755-61 Abstract
  • Chapkin RS, Hong MY, Fan YY, Davidson LA, Sanders LM, Henderson CE, Barhoumi R, Burghardt RC, Turner ND, Lupton JR (Feb 2002) Dietary n-3 PUFA alter colonocyte mitochondrial membrane composition and function., Lipids, 37 (2), 193-9 Abstract
  • Rigby SL, Barhoumi R, Burghardt RC, Colleran P, Thompson JA, Varner DD, Blanchard TL, Brinsko SP, Taylor T, Wilkerson MK, Delp MD (Sep 2001) Mares with delayed uterine clearance have an intrinsic defect in myometrial function., Biology of reproduction, 65 (3), 740-7 Abstract
  • Reeves WR, Barhoumi R, Burghardt RC, Lemke SL, Mayura K, McDonald TJ, Phillips TD, Donnelly KC (Apr 2001) Evaluation of methods for predicting the toxicity of polycyclic aromatic hydrocarbon mixtures., Environmental science & technology, 35 (8), 1630-6 Abstract
  • Samudio I, Vyhlidal C, Wang F, Stoner M, Chen I, Kladde M, Barhoumi R, Burghardt R, Safe S (Mar 2001) Transcriptional activation of deoxyribonucleic acid polymerase alpha gene expression in MCF-7 cells by 17 beta-estradiol., Endocrinology, 142 (3), 1000-8 Abstract
  • Legare ME, Hanneman WH, Barhoumi R, Burghardt RC, Tiffany-Castiglioni E (Dec 2000) 2,3,7,8-Tetrachlorodibenzo-p-dioxin alters hippocampal astroglia-neuronal gap junctional communication., Neurotoxicology, 21 (6), 1109-16 Abstract
  • Barhoumi R, Mouneimne Y, Ramos KS, Safe SH, Phillips TD, Centonze VE, Ainley C, Gupta MS, Burghardt RC (Feb 2000) Analysis of benzo[a]pyrene partitioning and cellular homeostasis in a rat liver cell line., Toxicological sciences : an official journal of the Society of Toxicology, 53 (2), 264-70 Abstract
  • Mnaimneh S, Damaj M, Barhoumi R, Mouneimne Y, Veyret B, Geffard M, Vincendeau P, Circulating antibodies directed against nitrosylated antigens in trypanosome-infected mice., Experimental parasitology, 105 (3-4), 241-7 Abstract

Profile Details

Last Verified: 11/6/2008

COS Expertise ID #104774
Reference this profile directly: http://myprofile.cos.com/rmouneimne