Dr. Robert J. Talmadge

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California Polytechnic State University
College of Science
Biological Sciences
Assistant ProfessorAppointed: 2001

Mailing Address

Biological Sciences
California State Polytechnic University, Pomona
3801 West Temple Ave.
Pomona, California 91768
United States

Contact Information

Phone: (909) 869-3025
Fax: (909) 869-4078
rjtalmadge@csupomona.edu

Qualifications

Ph.D., Louisiana State University, 1991.

Expertise and Research Interests

The primary goal of my research program is to understand the molecular processes associated with skeletal muscle dysfunction following reduced activation induced by spinal cord injury or other debilitating conditions (such as space flight, congestive heart failure, or immobilization). Chronic reductions in muscle activity result in atrophic conditions, which severely compromise the maximal amounts of force a muscle can produce, as well as, phenotypic adaptations, which alter the physiological profile ofthe muscle. Typically, the muscle becomes faster and less fatigue resistant following chronic reductions in activation. These adaptations are accompanied by alterations in expression of many proteins and protein isoforms associated with contractile function and energy (ATP) production. Specifically, our laboratory is interested in determining the signaling mechanisms associated with the activity-based regulation of muscle phenotype (fast vs. slow contracting) and fatigue resistance. Presently, our studies utilize transgenic mice that express a constitutively active form of calcineurin (courtesy of Dr. Eric Olson, UT Southwestern Medical Center), a calcium activated phosphatase that is thought to transduce activity-based signals (calcium transients) into molecular signals (alterations in the types and amounts of transcription factors in the muscle fiber nucleus). The constitutively active form of calcineurin is not regulated by calcium and allows for a determination of the extent of calcineurin (andcalcium transient) involvement in these signaling processes.

Other projects in the laboratory include: (1) determining the cellular mechanisms associated with muscular dystrophy; (2) understanding the mechanisms associated with sarcopenia; and (3) elucidating the cellular adaptations in the lumbar spinal cord locomotor networks following spinal cord injury.

Other Expertise

Technical expertise in SDS-PAGE, fluorescence and confocal microscopy, Southern, Northern and Western blotting, quantitative histochemistry, and microscopy image analysis.

Keywords

COS Keywords:

Immunohistochemistry, Nutrition or Dietetics, Spinal Cord Injury.

Additional Terms:

Calcineurin, Muscle Plasticity, Myosin, Sarcopenia, Spaceflight, Spinal Cord Injury.

Memberships

American Association for the Advancement of Science
American College of Sports Medicine
American Physiological Society

Funding Received

  • Jeffress Memorial Trust (With R.W. Grange as co-PI): Role of the ATP-dependent ubiquitin-proteasome pathway in cell cycle regulation of normal and dystrophic muscle., $30,000, Jul 1, 2000 to Jun 30, 2001.

Publications

  • Talmadge RJ, Castro MJ, Apple DF Jr, Dudley GA., Phenotypic adaptations in human muscle fibers 6 and 24 wk after spinal cord injury, Journal of Applied Physiology, 92(1), 147-154, January 2002
  • Spangenburg EE, Lees SJ, Otis JS, Musch TI, Talmadge RJ, Williams JH, Effects of moderate heart failure and functional overload on rat plantaris muscle, Journal of Applied Physiology, 92(1), 18-24, January 2002
  • Aschenbach WG, Brower GL, Talmadge RJ, Dobson JL, and Gladden LB, Effect of a myocardial volume overload on lactate transport in skeletal muscle sarcolemmal vesicles, American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 281(1), R176-R186, July 2001
  • Oishi Y., Ishihara A, Talmadge RJ, Ohira Y, Taniguchi K, Matsumoto H, Roy RR, and Edgerton VR, Expression of HSP72 in atrophied rat skeletal muscles, Acta Physiologica Scandinavica, 172(2), 123 - 130, June 2001
  • Edgerton VR, de Leon R.D, Harkema SJ, Hodgson JA, London N, Reinkensmeyer DJ, Roy RR, Talmadge RJ, Tillakaratne NJ, Timoszyk W, and Tobin A, Retraining the injured spinal cord, Journal of Physiology (London), 533(1), 15 - 22, May 2001
  • Spangenburg EE, Williams JH, Roy RR, and Talmadge RJ, Skeletal muscle calcineurin: influence of phenotype adaptation and atrophy, American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 280(4), R1256-R1260, April 2001
  • Talmadge RJ, Myosin heavy chain isoform expression following reduced neuromuscular activity: potential regulatory mechanisms, Muscle and Nerve, 23(5), 661-79, May 2000 Abstract
  • Roy RR, Kim JA, Grossman EJ, Bekmezian A, Talmadge RJ, Zhong H, Edgerton VR, Persistence of myosin heavy chain-based fiber types in innervated but silenced rat fast muscle, Muscle and Nerve, 23(5), 735-47, May 2000 Abstract
  • Unguez GA, Talmadge RJ, Roy RR, Dalponte D, Edgerton VR, Distinct myosin heavy chain isoform transitions in developing slow and fast cat hindlimb muscles, Cells, Tissues, Organs, 167(2-3), 138-52, 2000 Abstract
  • Talmadge RJ, Roy RR, Edgerton VR, Persistence of hybrid fibers in rat soleus after spinal cord transection, Anatomical Record, 255(2), 188-201, June 1999 Abstract
  • Rivero JL, Talmadge RJ, Edgerton VR, Interrelationships of myofibrillar ATPase activity and metabolic properties of myosin heavy chain-based fibre types in rat skeletal muscle, Histochemistry and Cell Biology, 111(4), 277-87, April 1999 Abstract
  • Bamman MM, Clarke MS, Talmadge RJ, Feeback DL, Enhanced protein electrophoresis technique for separating human skeletal muscle myosin heavy chain isoforms, Electrophoresis, 20(3), 466-8, March 1999 Abstract
  • Roy RR, Talmadge RJ, Hodgson JA, Oishi Y, Baldwin KM, Edgerton VR, Differential response of fast hindlimb extensor and flexor muscles to exercise in adult spinalized cats, Muscle and Nerve, 22(2), 230-41, February 1999 Abstract
  • Roy, R.R., S.C. Bodine, D.J. Pierotti, J.A. Kim, R.J. Talmadge, G. Barkhoudarian, J.W. Fanton, I. Koslovskaya, and V.R. Edgerton., Fiber size and myosin phenotypes of selected rhesus hindlimb muscles after a 14-day spaceflight, Journal of Gravitational Physiology, 6(2), 55-62, 1999
  • Rivero JL, Talmadge RJ, Edgerton VR, Fibre size and metabolic properties of myosin heavy chain-based fibre types in rat skeletal muscle, Journal of Muscle Research and Cell Motility, 19(7), 733-42, October 1998 Abstract
  • Grossman EJ, Roy RR, Talmadge RJ, Zhong H, Edgerton VR, Effects of inactivity on myosin heavy chain composition and size of rat soleus fibers, Muscle and Nerve, 21(3), 375-89, March 1998 Abstract
  • Roy RR, Talmadge RJ, Hodgson JA, Zhong H, Baldwin KM, Edgerton VR, Training effects on soleus of cats spinal cord transected (T12-13) as adults, Muscle and Nerve, 21(1), 63-71, Jan 1998 Abstract
  • Bamman MM, Clarke MS, Feeback DL, Talmadge RJ, Stevens BR, Lieberman SA, Greenisen MC, Impact of resistance exercise during bed rest on skeletal muscle sarcopenia and myosin isoform distribution, Journal of Applied Physiology, 84(1), 157-63, Jan 1998 Abstract
  • Grossman EJ, Grindeland RE, Roy RR, Talmadge RJ, Evans J, Edgerton VR, Growth hormone, IGF-I, and exercise effects on non-weight-bearing fast muscles of hypophysectomized rats, Journal of Applied Physiology, 83(5), 1522-30, November 1997 Abstract
  • Rivero JL, Talmadge RJ, Edgerton VR, A sensitive electrophoretic method for the quantification of myosin heavy chain isoforms in horse skeletal muscle: histochemical and immunocytochemical verifications, Electrophoresis, 18(11), 1967-72, October 1997 Abstract
  • Roy RR, Talmadge RJ, Fox K, Lee M, Ishihara A, Edgerton VR, Modulation of MHC isoforms in functionally overloaded and exercised rat plantaris fibers, Journal of Applied Physiology, 83(1), 280-90, July 1997 Abstract
  • Ishihara A, Hori A, Roy RR, Oishi Y, Talmadge RJ, Ohira Y, Kobayashi S, Edgerton VR, Perineal muscles and their innervation: metabolic and functional significance of the motor unit, Acta Anatomica, 159(2-3), 156-66, 1997 Abstract
  • Talmadge RJ, Roy RR, Edgerton VR, Distribution of myosin heavy chain isoforms in non-weight-bearing rat soleus muscle fibers, Journal of Applied Physiology, 81(6), 2540-6, December 1996 Abstract
  • Rivero JL, Talmadge RJ, Edgerton VR, Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study, Anatomical Record, 246(2), 185-94, October 1996 Abstract
  • Rivero JL, Talmadge RJ, Edgerton VR, Correlation between myofibrillar ATPase activity and myosin heavy chain composition in equine skeletal muscle and the influence of training, Anatomical Record, 246(2), 195-207, October 1996 Abstract
  • Roy RR, Roy ME, Talmadge RJ, Mendoza R, Grindeland RE, Vasques M, Size and myosin heavy chain profiles of rat hindlimb extensor muscle fibers after 2 weeks at 2G, Aviation Space and Environmental Medicine, 67(9), 854-8, September 1996 Abstract
  • Talmadge RJ, Roy RR, Edgerton VR, Myosin heavy chain profile of cat soleus following chronic reduced activity or inactivity, Muscle and Nerve, 19(8), 980-8, August 1996 Abstract
  • Talmadge RJ, Grossman EJ, Roy RR, Myosin heavy chain composition of adult feline (Felis catus) limb and diaphragm muscles, Journal of Experimental Zoology, 275(6), 413-20, August 1996 Abstract
  • Roy RR, Tri C, Grossman EJ, Talmadge RJ, Grindeland RE, Mukku VR, Edgerton VR, IGF-I, growth hormone, and/or exercise effects on non-weight-bearing soleus of hypophysectomized rats, Journal of Applied Physiology, 81(1), 302-11, July 1996 Abstract
  • Talmadge RJ, Roy RR, Chalmers GR, Edgerton VR, MHC and sarcoplasmic reticulum protein isoforms in functionally overloaded cat plantaris muscle fibers, Journal of Applied Physiology, 80(4), 1296-303, April 1996 Abstract
  • Linderman JK, Talmadge RJ, Gosselink KL, Tri PN, Roy RR, Grindeland RE, Synergistic ablation does not affect atrophy or altered myosin heavy chain expression in the non-weight bearing soleus muscle, Life Sciences, 59(10), 789-95, 1996 Abstract
  • Talmadge RJ, Roy RR, Jiang B, Edgerton VR, Myofibrillar ATPase activity of feline muscle fibers expressing slow and fast myosin heavy chains, Journal of Histochemistry and Cytochemistry, 43(8), 811-9, August 1995 Abstract
  • Allen DL, Monke SR, Talmadge RJ, Roy RR, Edgerton VR, Plasticity of myonuclear number in hypertrophied and atrophied mammalian skeletal muscle fibers, Journal of Applied Physiology, 78(5), 1969-76, May 1995 Abstract
  • Talmadge RJ, Roy RR, Edgerton VR, Prominence of myosin heavy chain hybrid fibers in soleus muscle of spinal cord-transected rats, Journal of Applied Physiology, 78(4), 1256-65, April 1995 Abstract
  • Talmadge, R.J., R. R. Roy, S.C. Bodine-Fowler, D.J. Pierotti, and V.R. Edgerton., Adaptations in myosin heavy chain profile in chronically unloaded muscles, Basic and Applied Myology, 5(2), 117-137, 1995
  • Bodine-Fowler, S.C., D.J. Pierotti, and R.J. Talmadge, Functional and Cellular adaptation to weightlessness in primates, Journal of Gravitational Physiology, 2(1), P43-P46, 1995
  • Talmadge RJ, Mills S, Powers SK, Silverman H, Limited capacity for glyconeogenesis from alanine by diaphragm muscle, Respiration Physiology, 98(2), 153-64, October 1994 Abstract
  • Talmadge RJ, Roy RR, Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms, Journal of Applied Physiology, 75(5), 2337-40, November 1993 Abstract
  • Talmadge RJ, Roy RR, Edgerton VR, Muscle fiber types and function, Current Opinion in Rheumatology, 5(6), 695-705, November 1993 Abstract
  • Talmadge RJ, Silverman H, Glucose uptake and glycogen synthesis in normal and chronically active muscles, American Journal of Physiology, 264(3 Pt 1), E328-33, March 1993 Abstract
  • Talmadge RJ, Silverman H, Glyconeogenic and glycogenic enzymes in chronically active and normal skeletal muscle, Journal of Applied Physiology, 71(1), 182-91, July 1991 Abstract
  • Talmadge RJ, Scheide JI, Silverman H, Glycogen synthesis from lactate in a chronically active muscle, Journal of Applied Physiology, 66(5), 2231-8, May 1989 Abstract
  • Hargroder GT, Talmadge RJ, Silverman H, Age-related changes in oxidative capacity of the gastrocnemius muscle in normal and dystrophic (dy2J/dy2J) mice, Experimental Neurology, 94(2), 400-15, November 1986 Abstract

Profile Details

Last Updated: 12/26/2001

COS Expertise ID #844697
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