Prof. Eric Wickstrom |
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Thomas Jefferson University Jefferson Medical College Biochemistry & Molecular Biology Nucleic Acid Therapeutics ProfessorAppointed: 2005 Thomas Jefferson University Thomas Jefferson University Hospital Biochemistry & Molecular Pharmacology |  |
Mailing AddressLaboratory of Nucleic Acid Therapeutics Biochemistry and Molecular Biology Thomas Jefferson University 233 S. 10th Street, Bluemle 219 Philadelphia, Pennsylvania 19107-5587United States | Contact Information |
Qualifications
Ph.D., University of California, Berkeley, Chemistry, 1972.
B.S. with honors, California Institute of Technology, Biology, 1968.
Expertise and Research Interests
Cancer covers a broad spectrum of diseases, in every tissue of the body. Tissues are composed of cells, which normally grow slowly, under the tight control of a network of regulatory genes. The slow accumulation of activating mutations in growth genes, and inactivating mutations in suppressor genes, eventually allows a cell to grow out of control. Relapse is due to the development of resistant cells, rather than the escape of sensitive cells, suggesting the need for new approaches to treatment of the disease.
This laboratory is developing gene-specific oligonucleotides and siRNAs against oncogenes in the signal transduction pathway and against viral genes for use as diagnostics and therapeutics for cancers. The biological systems being studied include the CCND1 oncogene, MYC oncogene, HER2 oncogene, and KRAS oncogene in breast cancer, ovarian cancer, pancreatic cancer, colon cancer and lung cancer. We also study the roles of developmental genes and oncogene orthologs in zebrafish.
For this approach to succeed, the most efficacious antisense and siRNA target sequences must be identified, the mechanisms and physiological effects must be elucidated, and potent DNA and RNA analogs capable of surviving in the bloodstream following administration must be synthesized, and their structures must be determined.
Cell-type-specific peptide analogs are being synthesized to enable cell-type-specific uptake, hybridization, and steric blocking activity of peptide nucleic acids. By adding a radionuclide chelator on one end, and a peptide ligand analog on the other end, gene expression in tumors can be visualized from outside the body. By using a branched, dendrimer with multiple chelators to bind gadolinium, we can extend this mRNA visualization method to magnetic resonance imaging. We also use negatively charged peptide nucleic acids for reverse genetic studies of oncogene orthologs in zebrafish.
For nonviral gene therapy, methods are being developed to allow gene insertion at defined sequences in diseased cells, using the conserved recognition sequence of transposon Tn7. To apply this system in higher organisms, the protein-DNA interactions regulating transposition must be elucidated. This will permit genetic therapy by a biological route, in addition to the chemical route being pursued with synthetic oligonucleotides.
Infections that develop on medical implants inflict great damage. We are interdicting infections before they start by covalently bonding therapeutics, such as antibiotics, peptides, or oligonucleotides, to titanium and other implant materials.
This laboratory is developing gene-specific oligonucleotides and siRNAs against oncogenes in the signal transduction pathway and against viral genes for use as diagnostics and therapeutics for cancers. The biological systems being studied include the CCND1 oncogene, MYC oncogene, HER2 oncogene, and KRAS oncogene in breast cancer, ovarian cancer, pancreatic cancer, colon cancer and lung cancer. We also study the roles of developmental genes and oncogene orthologs in zebrafish.
For this approach to succeed, the most efficacious antisense and siRNA target sequences must be identified, the mechanisms and physiological effects must be elucidated, and potent DNA and RNA analogs capable of surviving in the bloodstream following administration must be synthesized, and their structures must be determined.
Cell-type-specific peptide analogs are being synthesized to enable cell-type-specific uptake, hybridization, and steric blocking activity of peptide nucleic acids. By adding a radionuclide chelator on one end, and a peptide ligand analog on the other end, gene expression in tumors can be visualized from outside the body. By using a branched, dendrimer with multiple chelators to bind gadolinium, we can extend this mRNA visualization method to magnetic resonance imaging. We also use negatively charged peptide nucleic acids for reverse genetic studies of oncogene orthologs in zebrafish.
For nonviral gene therapy, methods are being developed to allow gene insertion at defined sequences in diseased cells, using the conserved recognition sequence of transposon Tn7. To apply this system in higher organisms, the protein-DNA interactions regulating transposition must be elucidated. This will permit genetic therapy by a biological route, in addition to the chemical route being pursued with synthetic oligonucleotides.
Infections that develop on medical implants inflict great damage. We are interdicting infections before they start by covalently bonding therapeutics, such as antibiotics, peptides, or oligonucleotides, to titanium and other implant materials.
Other Expertise
Experience:
Jefferson Medical College, Thomas Jefferson University
1992-1996: Professor of Pharmacology; Member, Jefferson Cancer Institute
1997-present: Professor of Microbiology and Immunology; Member, Kimmel Cancer Center; Member, Cardeza Foundation for Hematologic Research
2002-2005: Professor of Biochemistry and Molecular Pharmacology
Teaching (1992-present):
Graduate: BI 515 General Biochemistry I; BI 614 Protein Structure and Function; BI 522 Experimental Principles in Molecular Biology; BI/PR 710,720,730 Biochemical/Pharmacological Literature Seminar; GE 652 Molecular Basis of Cancer; PR 613 Structural Biology I; BI 614 Structural Biology II; TE 521 Tissue Engineering
University, College, and Departmental Committee Service (1992-present):
Thomas Jefferson University
Clinical Cancer Research Review Committee, 1993-present
Animal Resources Committee, 1997-8
Jefferson Medical College
Faculty Affairs Committee, 2004-present
Research Committee, 2005-present
Kimmel Cancer Center
CD Facility Committee, 2000-present
Development Therapeutics Committee, 1997-present
NMR Facility Committee, 1993-2002
Computer Committee, 1993-7
Structural Biology Committee, 1993-7
Invited Lectures (2003-present):
1. Tumor diagnosis by SPECT and PET imaging of cancer gene mRNA. Penn Cancer Genetics Seminar, Department of Pediatrics, University of Pennsylvania, Philadelphia, Pennsylvania, 2005.
2. Imaging cancer gene mRNA in tumors. Distinguished Lecture in Macromolecular Therapeutics, Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina, 2005.
3. Gamma and positron imaging of oncogene mRNA inside tumors from outside the body with radionuclide-chelator-PNA-peptide chimeras. Department of Chemistry, Hunter College, City University of New York, New York, New York, 2005.
4. External imaging of oncogene mRNAs in tumors. Optical Imaging Retreat, Abramson Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania, 2005.
5. Noninvasive imaging of KRAS oncogene mRNAs in pancreatic cancer xenografts with [111In]DOTA-dendrimer-PNA-peptide chimeras. Pancreas Cancer 2005: State-of-the-Art, Memorial Sloan-Kettering Cancer Center, New York, New York, 2005.
6. Radionuclide-chelator-PNA-peptides for imaging cancer gene mRNAs in tumors. Department of Chemical Engineering, University of Delaware, Newark, Delaware, 2005.
7. Hydroxyprolyl/phosphono peptide nucleic acid inhibition of ccnd1 mRNA in developing zebrafish embryos: developmental consequences of cyclin D1 reduction. 1st International Symposium on Biomolecules and Related Compounds: Chemistry, Biology and Applications, Montpellier, France, 2005.
8. Noninvasive detection of oncogene overexpression by gamma and positron imaging of mRNA in tumors. Department of Radiation Oncology, University of Pennsylvania, Philadelphia, Pennsylvania, 2005.
9. External diagnosis of oncogene activation in tumors by radioimaging. Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 2004.
10. External imaging of CCND1, MYC and KRAS oncogene mRNAs with tumor-targeted radionuclide-PNA-peptide chimeras. International Conference on Tumor Progression and Therapeutic Resistance, Philadelphia, Pennsylvania, 2004.
11. Gamma and positron imaging of oncogene mRNA inside tumors from outside the body. Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, 2004.
12. Peptide-PNA-radionuclide chimeras for molecular diagnosis of cancer. DOE Gene Imaging Program, Chicago, Illinois, 2004.
13. Antisense nanoparticles for imaging, treatment, and assessment of pancreatic cancer gene expression. NASA/NCI Unconventional Innovations Program, San Diego, California, 2004.
14. Peptide-PNA-radionuclide chimeras for molecular diagnosis of cancer. 5th Cambridge Symposium on Nucleic Acids Chemistry and Biology, Queens' College, Cambridge, England, 2003.
15. Zebrafish embryonic gene knockdown with alternating hydroxyprolyl/phosphono peptide nucleic acids. Gordon Research Conference on Purines, Pyrimidines & Related Substances, Salve Regina University, Newport, Rhode Island, 2003.
Conference Presentations (2005-present):
1. Tian, X., Aruva, M.R., Zhang, K., Mathew, B., Cardi, C., Thakur, M. L., and Wickstrom, E. (2005) microPET/CT imaging of human breast cancer xenografts in immunocompromised mice with tumor-specific PNA-peptide chimeras for targeting CCND1 oncogene mRNA. 4th Annual Meeting of the Society for Molecular Imaging, Cologne, Germany.
2. Amirkhanov, N., Aruva, M. R., Zhang, K., Mathew, B., Thakur, M. L. and Wickstrom, E. (2005) In-111- and Gd-157-DOTA-polydiamidopropanoate (PDAP) dendrimer-PNA-peptide chimeras for noninvasive imaging of intracellular oncogene mRNA expression. 1st International Conference on Chemical Biology, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
3. Antoci, V., King, S., Parvizi, J., Adams, C., Jose, B., Wickstrom, E., Zeiger, A., Shapiro, I., and Hickok, N. (2005) Vancomycin tethered to Ti rods prevents S. aureus biofilm formation. Eastern Orthopaedic Association 36th Annual Meeting, Chesapeake, Maryland.
4. Amirkhanov, N., Aruva, M. R., Zhang, K., Mathew, B., Thakur, M. L. and Wickstrom, E. (2005) Design, synthesis, and evaluation of chelator-dendrimer-peptide nucleic acid-peptide chimeras for non-invasive magnetic resonance imaging (MRI) of intracellular messenger RNA and oncogene expression. Gordon Research Conference on Nucleosides, Nucleotides, and Oligonucleotides, Salve Regina University, Newport, Rhode Island.
5. Zhang, K., Aruva, M. R., Mathew, B., Chakrabarti, A., Wickstrom, E., and Thakur, M. L. (2005) PET imaging of pancreatic cancer using Cu-64 labeled DOTA-PNA-peptide chimera. 16th International Symposium on Radiopharmaceutical Chemistry, Iowa City, Iowa.
6. Amirkhanov, N., Aruva, M. R., Zhang, K., Mathew, B., Thakur, M. L. and Wickstrom, E. (2005) Synthesis and evaluation of In-111 DOTA-polydiamidopropanoate (PDAP) dendrimer-PNA-peptide chimeras for non-invasive imaging of cancer. 16th International Symposium on Radiopharmaceutical Chemistry, Iowa City, Iowa.
7. Chakrabarti, A., Aruva, M. R., Zhang, K., Mathew, B., Fong, D.T., Thakur, M. L. and Wickstrom, E., (2005) External imaging of oncogene KRAS mRNA overexpression in pancreatic cancer xenografts with Tc-99m and Cu-64 chelator PNA-peptide chimeras. Society of Nuclear Medicine 52nd Annual Meeting, Toronto, Canada.
8. Teker, K., Sivakumar, K., Wickstrom, E., and Panchapakesan, B. (2005) Electronic sensing of antibodies using carbon nanotube devices. 2005 Nano Science and Technology Institute Nanotechnology Conference, Anaheim, California.
9. Jose, B., King, S. J., Zeiger, A. R., Adams, C. J., Hickok, N., and Wickstrom, E. (2005) Solid phase synthesis of vancomycin-bis(8-amido-3,6-dioxaoctanoyl)-amidopropylsiloxy-titanium implants that inhibit proliferation of Staphylococcus aureus. 15th Biennial Philadelphia Organic ChemistsÂ’ Club Day, Philadelphia, Pennsylvania.
10. Amirkhanov, N., Aruva, M. R., Zhang, K., Mathew, B., Thakur, M. L., and Wickstrom, E. (2005) In-111 and Gd-157 DOTA-polydiamidopropanoate (PDAP) dendrimer-PNA-peptide chimeras for non-invasive imaging of cancer. American Association for Cancer Research 96th Annual Meeting, Anaheim, California.
11. Chakrabarti, A., Aruva, M. R., Sajankila, S.P., Thakur, M. L., and Wickstrom, E. (2005) Tc-99m and Cu-64 PNA-peptide hybridization probes for imaging of oncogene activation in pancreatic cancer. American Association for Cancer Research 96th Annual Meeting, Anaheim, California.
12. Jose, B., King, S. J., Zeiger, A. R., Hickok, N. J., and Wickstrom, E. (2005) Solid phase synthesis of vancomycin-bis(8-amido-3,6-dioxaoctanoyl)-amidopropylsiloxy-titanium implants that inhibit proliferation of Staphylococcus aureus. American Chemical Society 229th National Meeting, San Diego, California, Medicinal Chemistry.
13. Neuman, B., Hickok, N. J., Adams, C.S., Jose, B., and Wickstrom, E. (2005) The effect of Arg-Gly-Asp-Ser (RGDS) on osteosarcoma cell attachment to titanium alloy. Sigma Xi Society Meeting, Thomas Jefferson University, Philadelphia, Pennsylvania.
14. Cesarone, G., Garofalo, C., Igoucheva, O., Alexeev, V., Yoon, K., Surmacz, E., and Wickstrom, E. (2005) Specific reduction of insulin receptor substrate 1 (IRS-1) expression in MCF7 cells by RNA interference. Sigma Xi Society Meeting, Thomas Jefferson University, Philadelphia, Pennsylvania.
Jefferson Medical College, Thomas Jefferson University
1992-1996: Professor of Pharmacology; Member, Jefferson Cancer Institute
1997-present: Professor of Microbiology and Immunology; Member, Kimmel Cancer Center; Member, Cardeza Foundation for Hematologic Research
2002-2005: Professor of Biochemistry and Molecular Pharmacology
Teaching (1992-present):
Graduate: BI 515 General Biochemistry I; BI 614 Protein Structure and Function; BI 522 Experimental Principles in Molecular Biology; BI/PR 710,720,730 Biochemical/Pharmacological Literature Seminar; GE 652 Molecular Basis of Cancer; PR 613 Structural Biology I; BI 614 Structural Biology II; TE 521 Tissue Engineering
University, College, and Departmental Committee Service (1992-present):
Thomas Jefferson University
Clinical Cancer Research Review Committee, 1993-present
Animal Resources Committee, 1997-8
Jefferson Medical College
Faculty Affairs Committee, 2004-present
Research Committee, 2005-present
Kimmel Cancer Center
CD Facility Committee, 2000-present
Development Therapeutics Committee, 1997-present
NMR Facility Committee, 1993-2002
Computer Committee, 1993-7
Structural Biology Committee, 1993-7
Invited Lectures (2003-present):
1. Tumor diagnosis by SPECT and PET imaging of cancer gene mRNA. Penn Cancer Genetics Seminar, Department of Pediatrics, University of Pennsylvania, Philadelphia, Pennsylvania, 2005.
2. Imaging cancer gene mRNA in tumors. Distinguished Lecture in Macromolecular Therapeutics, Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina, 2005.
3. Gamma and positron imaging of oncogene mRNA inside tumors from outside the body with radionuclide-chelator-PNA-peptide chimeras. Department of Chemistry, Hunter College, City University of New York, New York, New York, 2005.
4. External imaging of oncogene mRNAs in tumors. Optical Imaging Retreat, Abramson Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania, 2005.
5. Noninvasive imaging of KRAS oncogene mRNAs in pancreatic cancer xenografts with [111In]DOTA-dendrimer-PNA-peptide chimeras. Pancreas Cancer 2005: State-of-the-Art, Memorial Sloan-Kettering Cancer Center, New York, New York, 2005.
6. Radionuclide-chelator-PNA-peptides for imaging cancer gene mRNAs in tumors. Department of Chemical Engineering, University of Delaware, Newark, Delaware, 2005.
7. Hydroxyprolyl/phosphono peptide nucleic acid inhibition of ccnd1 mRNA in developing zebrafish embryos: developmental consequences of cyclin D1 reduction. 1st International Symposium on Biomolecules and Related Compounds: Chemistry, Biology and Applications, Montpellier, France, 2005.
8. Noninvasive detection of oncogene overexpression by gamma and positron imaging of mRNA in tumors. Department of Radiation Oncology, University of Pennsylvania, Philadelphia, Pennsylvania, 2005.
9. External diagnosis of oncogene activation in tumors by radioimaging. Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 2004.
10. External imaging of CCND1, MYC and KRAS oncogene mRNAs with tumor-targeted radionuclide-PNA-peptide chimeras. International Conference on Tumor Progression and Therapeutic Resistance, Philadelphia, Pennsylvania, 2004.
11. Gamma and positron imaging of oncogene mRNA inside tumors from outside the body. Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, 2004.
12. Peptide-PNA-radionuclide chimeras for molecular diagnosis of cancer. DOE Gene Imaging Program, Chicago, Illinois, 2004.
13. Antisense nanoparticles for imaging, treatment, and assessment of pancreatic cancer gene expression. NASA/NCI Unconventional Innovations Program, San Diego, California, 2004.
14. Peptide-PNA-radionuclide chimeras for molecular diagnosis of cancer. 5th Cambridge Symposium on Nucleic Acids Chemistry and Biology, Queens' College, Cambridge, England, 2003.
15. Zebrafish embryonic gene knockdown with alternating hydroxyprolyl/phosphono peptide nucleic acids. Gordon Research Conference on Purines, Pyrimidines & Related Substances, Salve Regina University, Newport, Rhode Island, 2003.
Conference Presentations (2005-present):
1. Tian, X., Aruva, M.R., Zhang, K., Mathew, B., Cardi, C., Thakur, M. L., and Wickstrom, E. (2005) microPET/CT imaging of human breast cancer xenografts in immunocompromised mice with tumor-specific PNA-peptide chimeras for targeting CCND1 oncogene mRNA. 4th Annual Meeting of the Society for Molecular Imaging, Cologne, Germany.
2. Amirkhanov, N., Aruva, M. R., Zhang, K., Mathew, B., Thakur, M. L. and Wickstrom, E. (2005) In-111- and Gd-157-DOTA-polydiamidopropanoate (PDAP) dendrimer-PNA-peptide chimeras for noninvasive imaging of intracellular oncogene mRNA expression. 1st International Conference on Chemical Biology, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
3. Antoci, V., King, S., Parvizi, J., Adams, C., Jose, B., Wickstrom, E., Zeiger, A., Shapiro, I., and Hickok, N. (2005) Vancomycin tethered to Ti rods prevents S. aureus biofilm formation. Eastern Orthopaedic Association 36th Annual Meeting, Chesapeake, Maryland.
4. Amirkhanov, N., Aruva, M. R., Zhang, K., Mathew, B., Thakur, M. L. and Wickstrom, E. (2005) Design, synthesis, and evaluation of chelator-dendrimer-peptide nucleic acid-peptide chimeras for non-invasive magnetic resonance imaging (MRI) of intracellular messenger RNA and oncogene expression. Gordon Research Conference on Nucleosides, Nucleotides, and Oligonucleotides, Salve Regina University, Newport, Rhode Island.
5. Zhang, K., Aruva, M. R., Mathew, B., Chakrabarti, A., Wickstrom, E., and Thakur, M. L. (2005) PET imaging of pancreatic cancer using Cu-64 labeled DOTA-PNA-peptide chimera. 16th International Symposium on Radiopharmaceutical Chemistry, Iowa City, Iowa.
6. Amirkhanov, N., Aruva, M. R., Zhang, K., Mathew, B., Thakur, M. L. and Wickstrom, E. (2005) Synthesis and evaluation of In-111 DOTA-polydiamidopropanoate (PDAP) dendrimer-PNA-peptide chimeras for non-invasive imaging of cancer. 16th International Symposium on Radiopharmaceutical Chemistry, Iowa City, Iowa.
7. Chakrabarti, A., Aruva, M. R., Zhang, K., Mathew, B., Fong, D.T., Thakur, M. L. and Wickstrom, E., (2005) External imaging of oncogene KRAS mRNA overexpression in pancreatic cancer xenografts with Tc-99m and Cu-64 chelator PNA-peptide chimeras. Society of Nuclear Medicine 52nd Annual Meeting, Toronto, Canada.
8. Teker, K., Sivakumar, K., Wickstrom, E., and Panchapakesan, B. (2005) Electronic sensing of antibodies using carbon nanotube devices. 2005 Nano Science and Technology Institute Nanotechnology Conference, Anaheim, California.
9. Jose, B., King, S. J., Zeiger, A. R., Adams, C. J., Hickok, N., and Wickstrom, E. (2005) Solid phase synthesis of vancomycin-bis(8-amido-3,6-dioxaoctanoyl)-amidopropylsiloxy-titanium implants that inhibit proliferation of Staphylococcus aureus. 15th Biennial Philadelphia Organic ChemistsÂ’ Club Day, Philadelphia, Pennsylvania.
10. Amirkhanov, N., Aruva, M. R., Zhang, K., Mathew, B., Thakur, M. L., and Wickstrom, E. (2005) In-111 and Gd-157 DOTA-polydiamidopropanoate (PDAP) dendrimer-PNA-peptide chimeras for non-invasive imaging of cancer. American Association for Cancer Research 96th Annual Meeting, Anaheim, California.
11. Chakrabarti, A., Aruva, M. R., Sajankila, S.P., Thakur, M. L., and Wickstrom, E. (2005) Tc-99m and Cu-64 PNA-peptide hybridization probes for imaging of oncogene activation in pancreatic cancer. American Association for Cancer Research 96th Annual Meeting, Anaheim, California.
12. Jose, B., King, S. J., Zeiger, A. R., Hickok, N. J., and Wickstrom, E. (2005) Solid phase synthesis of vancomycin-bis(8-amido-3,6-dioxaoctanoyl)-amidopropylsiloxy-titanium implants that inhibit proliferation of Staphylococcus aureus. American Chemical Society 229th National Meeting, San Diego, California, Medicinal Chemistry.
13. Neuman, B., Hickok, N. J., Adams, C.S., Jose, B., and Wickstrom, E. (2005) The effect of Arg-Gly-Asp-Ser (RGDS) on osteosarcoma cell attachment to titanium alloy. Sigma Xi Society Meeting, Thomas Jefferson University, Philadelphia, Pennsylvania.
14. Cesarone, G., Garofalo, C., Igoucheva, O., Alexeev, V., Yoon, K., Surmacz, E., and Wickstrom, E. (2005) Specific reduction of insulin receptor substrate 1 (IRS-1) expression in MCF7 cells by RNA interference. Sigma Xi Society Meeting, Thomas Jefferson University, Philadelphia, Pennsylvania.
Future Research
Additional receptor ligand analogs will be attached to the termini of peptide nucleic acids, in order to accelerate cell-specific uptake and slow excretion.
Multiple routes of oligonucleotide administration are being explored, including subcutaneous injection, intraperitoneal injection, subcutaneous micro-osmotic pumps, and oral administration.
Multiple routes of oligonucleotide administration are being explored, including subcutaneous injection, intraperitoneal injection, subcutaneous micro-osmotic pumps, and oral administration.
Industrial Relevance
Oligonucleotide therapies for cancer, inflammation, and viruses
Oligonucleotide noninvasive diagnostics for cancer, inflammation, and viruses
Nonviral gene therapy by site-specific transposition for hemophilia, sickle cell anemia, muscular dystrophy, cancer, inflammation, and viruses
Self-protecting medical implants bearing covalently bound therapeutics
Oligonucleotide noninvasive diagnostics for cancer, inflammation, and viruses
Nonviral gene therapy by site-specific transposition for hemophilia, sickle cell anemia, muscular dystrophy, cancer, inflammation, and viruses
Self-protecting medical implants bearing covalently bound therapeutics
Keywords
COS Keywords:
Biochemistry, Breast Cancer, Cancer Biology, Cancer Or Carcinogenesis, Cancer Prevention, Cell Cycle, Cell Differentiation, Chemical Synthesis, DNA, Drug Delivery Systems, Gene Expression, Liquid Chromatography, Lymphocytes, Lymphoma, Magnetic Resonance Imaging, Mass Spectrometry, Messenger RNA, Molecular Biology, Nucleic Acid Hybridization, Ovarian Cancer, Pharmacokinetics, Stereochemistry, Transgenic Animals.Additional Terms:
Antineoplastic, Antisense Nucleic Acid, Athymic Mouse, B Lymphocyte, Breast Cancer, Cell Cycle, Cell Differentiation, Chemical Synthesis, Circular Dichroism, DNA, Drug Delivery System, Drug Design Synthesis Production, Gene Expression, Liquid Chromatography, Lymphoma, Magnetic Resonance Imaging, Mass Spectrometry, Messenger RNA, Neoplasm Cancer Chemotherapy, Neoplasm Cancer Genetics, Neoplasm Cancer Immunotherapy, Neoplasm Cancer Pharmacology, Neoplasm Cancer Radionuclide Therapy, Neoplasm Cancer Transplantation, Neoplastic Cell, Neoplastic Growth, Nucleic Acid Chemical Synthesis, Nucleic Acid Hybridization, Nucleic Acid Sequence, Oligonucleotide, Oncogene, Ovarian Cancer, Pancreas Cancer, Pharmacokinetics, Phosphonate, Stereochemistry, Thiophosphate, Transgenic Animal.Languages
(Reading, Writing, Speaking)
Russian: (Basic, Basic, Basic)
Memberships
American Association for Cancer Research
American Chemical Society
American Society for Biochemistry and Molecular Biology
International Society for Nucleosides, Nucleotides, and Nucleic Acids
Oligonucleotide Therapeutics Society
Previous Positions
2005, Professor,
Thomas Jefferson University,
Jefferson Medical College,
Biochemistry & Molecular Pharmacology
Patents
Antigenomic Oligonucleotides for Treatment of Infection by Negative-Stranded Nonsegmented RNA Viruses,
Patent Number: 6355621,
2002,
Institution-owned,
United States.
Peptide Nucleic Acid Conjugates,
Patent Number: 6180767,
2001,
Institution-owned,
United States of America.
Composition and Method for Targeted Integration into Cells,
Patent Number: 5958775,
1999,
Institution-owned,
United States of America.
Stereospecific Solid Phase Synthesis of Oligodeoxynucleoside Alkylphosphonates by Pentavalent Grignard Coupling,
Patent Number: 5703223,
1997,
Institution-owned,
United States of America.
Funding Received
- subcontract from the University of Pennsylvania: Magnetic Resonance and Near Infrared Imaging of KRAS mRNA, $155,000, 2006 to 2007.
- Biological Effects Research: Oncogene mRNA Imaging with Radionuclide-PNA-Peptides, ER63055, $328,725, 2003 to 2006.
- NCI N01-CO-27175-01: Radionuclide Imaging, Treatment, and Assessment of Cancer, $466,009, 2002 to 2006.
Publications
- Jose B, Antoci V Jr, Zeiger AR, Wickstrom E, Hickok NJ (Sep 2005) Vancomycin covalently bonded to titanium beads kills Staphylococcus
aureus., Chemistry & Biology, 12 (9), 1041-8
- Wickstrom E, Tian X, Amirkhanov NV, Chakrabarti A, Aruva MR, Rao PS, Qin W, Zhu W, Sauter ER, Thakur ML, Radionuclide-peptide Nucleic Acid in Diagnosis and Treatment of Pancreatic
Cancer., Methods in Molecular Medicine, 106, 135-91, 2005
- Amirkhanov NV, Wickstrom E (2005) Synthesis of novel polydiamidopropanoate dendrimer PNA-peptide chimeras
for non-invasive magnetic resonance imaging of cancer., Nucleosides, Nucleotides & Nucleic Acids, 24 (5-7), 423-6
- Duffy KT, McAleer MF, Davidson WR, Kari L, Kari C, Liu CG, Farber SA, Cheng KC, Mest JR, Wickstrom E, Dicker AP, Rodeck U (2005) Coordinate control of cell cycle regulatory genes in zebrafish development
tested by cyclin D1 knockdown with morpholino phosphorodiamidates and
hydroxyprolyl-phosphono peptide nucleic acids., Nucleic Acids Research, 33 (15), 4914-21
- Tian X, Aruva MR, Wolfe HR, Qin W, Sauter ER, Thakur ML, Waldman SA, Wickstrom E (2005) Tumor-targeting peptide-PNA-peptide chimeras for imaging overexpressed
oncogene mRNAs., Nucleosides, Nucleotides & Nucleic Acids, 24 (5-7), 1085-91
- Wickstrom E, Tian X, Amirkhanov NV, Chakrabarti A, Aruva MR, Rao PS, Qin W, Zhu W, Sauter ER, Thakur ML (2005) Radionuclide-peptide nucleic acid in diagnosis and treatment of pancreatic
cancer., Methods in Molecular Medicine, 106, 135-91
- Tian X, Aruva MR, Qin W, Zhu W, Sauter ER, Thakur ML, Wickstrom E, Noninvasive Molecular Imaging of MYC MRNA Expression in Human Breast
Cancer Xenografts With a [99mTc]peptide-peptide Nucleic Acid-peptide
Chimera., Bioconjugate Chemistry, 16(1), 70-9, Jan-Feb 2005
- Tian X, Aruva MR, Qin W, Zhu W, Sauter ER, Thakur ML, Wickstrom E (Jan-Feb 2005) Noninvasive molecular imaging of MYC mRNA expression in human breast
cancer xenografts with a [99mTc]peptide-peptide nucleic acid-peptide
chimera., Bioconjugate Chemistry, 16 (1), 70-9
- Tian X, Aruva MR, Qin W, Zhu W, Sauter ER, Thakur ML, Wickstrom E (Jan-Feb 2005) Noninvasive molecular imaging of MYC mRNA expression in human breast
cancer xenografts with a [99mTc]peptide-peptide nucleic acid-peptide
chimera., Bioconjugate Chemistry, 16 (1), 70-9
- Parvizi J, Wickstrom E, Zeiger AR, Adams CS, Shapiro IM, Purtill JJ, Sharkey PF, Hozack WJ, Rothman RH, Hickok NJ, Titanium Surface With Biologic Activity Against Infection., Clinical Orthopaedics and Related Research, 33-8, Dec 2004
- Tian X, Aruva MR, Qin W, Zhu W, Duffy KT, Sauter ER, Thakur ML, Wickstrom E, External Imaging of CCND1 Cancer Gene Activity in Experimental Human Breast Cancer Xenografts With 99mTc-peptide-peptide Nucleic Acid-peptide Chimeras., Journal of Nuclear Medicine, 45(12), 2070-82, Dec 2004
- Abrams MT, Robertson NM, Yoon K, Wickstrom E, Inhibition of Glucocorticoid-induced Apoptosis By Targeting the Major Splice Variants of BIM MRNA With Small Interfering RNA and Short Hairpin RNA., Journal of Biological Chemistry, 279(53), 55809-17, Dec 2004
- Thakur ML, Aruva MR, Gariepy J, Acton P, Rattan S, Prasad S, Wickstrom E, Alavi A, PET Imaging of Oncogene Overexpression Using 64Cu-vasoactive Intestinal Peptide (VIP) Analog: Comparison With 99mTc-VIP Analog, Journal of Nuclear Medicine, 45(8), 1381-9, Aug 2004
- Hargis MT, Storck CW, Wickstrom E, Yakubov LA, Leeper DB, Coss RA, Hsp27 Anti-sense Oligonucleotides Sensitize the Microtubular Cytoskeleton of Chinese Hamster Ovary Cells Grown At Low PH to 42 Degrees C-induced Reorganization., International Journal of Hyperthermia, 20(5), 491-502, Aug 2004
- Wickstrom E, Choob M, Urtishak KA, Tian X, Sternheim N, Talbot S, Archdeacon J, Efimov VA, Farber SA, Sequence Specificity of Alternating Hydroyprolyl/phosphono Peptide Nucleic
Acids Against Zebrafish Embryo MRNAs., Journal of Drug Targeting, 12(6), 363-72, Jul 2004
- Wickstrom E, Correspondence re: S. Heckl et al., Intracellular visualization of
prostate cancer using magnetic resonance imaging. Cancer Res
2003;63:4766-72, Cancer Research, 64(7), 2639; author reply 2, Apr 2004
- Chakrabarti A, Desai P, Wickstrom E, Transposon Tn7 protein TnsD binding to Escherichia coli attTn7 DNA and its
eukaryotic orthologs, Biochemistry, 43(10), 2941-6, Mar 2004
- Wickstrom E, Urtishak KA, Choob M, Tian X, Sternheim N, Cross LM, Rubinstein A, Farber SA, Downregulation of Gene Expression With Negatively Charged Peptide Nucleic
Acids (PNAs) in Zebrafish Embryos., Methods in Cell Biology, 77, 137-58, 2004
- Tian X, Aruva MR, Rao PS, Qin W, Read P, Sauter ER, Thakur ML, Wickstrom E, Imaging oncogene expression, Annals of the New York Academy of Sciences, 1002, 165-88, Dec 2003
- Vorobjev PE, Smith JB, Pyshnaya IA, Levina AS, Zarytova VF, Wickstrom E, Site-specific cleavage of RNA and DNA by complementary DNA--bleomycin A5
conjugates, Bioconjugate Chemistry, 14(6), 1307-13, Nov-D 2003
- Urtishak KA, Choob M, Tian X, Sternheim N, Talbot WS, Wickstrom E, Farber SA, Targeted Gene Knockdown in Zebrafish Using Negatively Charged Peptide Nucleic Acid Mimics, Developmental Dynamics, 228(3), 405-13, Nov 2003
- Rao PS, Tian X, Qin W, Aruva MR, Sauter ER, Thakur ML, Wickstrom E, 99mTc-peptide-peptide nucleic acid probes for imaging oncogene mRNAs in
tumours, Nuclear Medicine Communications, 24(8), 857-63, Aug 2003
- Butz J, Wickstrom E, Edwards J, Characterization of mutations and loss of heterozygosity of p53 and K-ras2
in pancreatic cancer cell lines by immobilized polymerase chain
reaction, Bmc Biotechnology [electronic Resource], 3(1), 11, Jul 2003
- Tian X, Wickstrom E, Continuous solid-phase synthesis and disulfide cyclization of
peptide-PNA-peptide chimeras, Organic Letters, 4(23), 4013-6, November 2002
- Vorob'ev PE, Pyshnyi DV, Wickstrom E, Zarytova VF, [Cleavage of RNA in a Hybrid Duplex By E. Coli Ribonuclease H. III. Substrate Characteristics of Hybrid Duplexes of RNA and Oligodeoxyribonucleotides Containing a Bleomycin A5 Residue, Bioorganicheskaia Khimiia, 28(4), 332-40, 2002
- Wickstrom E, Oligonucleotide treatment of ras-induced tumors in nude mice, Molecular Biotechnology, 18(1), 35-55, May 2001
- Lesnikowski, Z. J., Przepiórkiewicz, M., Tamura, Y., Kaji, H., and Wickstrom, E., Effect of configuration at phosphorus on transport of tetra(thymidine methylphosphonate)s across organic liquid membrane, Collection of Czechoslovak Chemical Communications, 66, 912-922, 2001
- Cleaver SH, Wickstrom E, Transposon Tn7 gene insertion into an evolutionarily conserved human
homolog of Escherichia coli attTn7, Gene, 254(1-2), 37-44, August 2000
- Smith JB, Wickstrom E, Preclinical antisense DNA therapy of cancer in mice, Methods in Enzymology, 314, 537-80, 2000
- Smith J B, Wickstrom E, Antisense c-myc and immunostimulatory oligonucleotide inhibition of tumorigenesis in a murine B-cell lymphoma transplant model., Journal of The National Cancer Institute, 90(15), 1146-54, 5 Aug 1998
- Wickstrom E, Smith J B, DNA combination therapy to stop tumor growth., Cancer Journal From Scientific American, 4 Suppl 1, S43-7, May 1998
- Smith JB, Wickstrom E, Inhibition of tumorigenesis in a murine B-cell lymphoma transplant model by c-Myc complementary oligonucleotides., Advanced Experimental Medicine and Biology, 451, 17-22, 1998
- Basu S, Wickstrom E, Synthesis and characterization of a peptide nucleic acid conjugated to a D-peptide analog of insulin-like growth factor 1 for increased cellular uptake., Bioconjugate Chemistry, 8(4), 481-8, July 1997
- Wickstrom E, Antisense c-myc inhibition of lymphoma growth., Antisense and Nucleic Acid Drug Development, 7(3), 225-8, June 1997
- Gray G D, Wickstrom E, Rapid measurement of modified oligonucleotide levels in plasma samples with a fluorophore specific for single-stranded DNA., Antisense and Nucleic Acid Drug Development, 7(3), 133-40, June 1997
- Gray G D, Basu S, Wickstrom E, Transformed and immortalized cellular uptake of oligodeoxynucleoside phosphorothioates, 3'-alkylamino oligodeoxynucleotides, 2'-O-methyl oligoribonucleotides, oligodeoxynucleoside methylphosphonates,, Biochemical Pharmacology, 53(10), 1465-76, 15 May 1997
- DeLong R K, Nolting A, Fisher M, Chen Q, Wickstrom E, Kligshteyn M, Demirdji S, Caruthers M, Juliano R L, Comparative pharmacokinetics, tissue distribution, and tumor accumulation of phosphorothioate, phosphorodithioate, and methylphosphonate oligonucleotides in nude mice., Antisense and Nucleic Acid Drug Development, 7(2), 71-7, April 1997
- Nolting A, DeLong R K, Fisher M H, Wickstrom E, Pollack G M, Juliano R L, Brouwer K L, Hepatic distribution and clearance of antisense oligonucleotides in the isolated perfused rat liver., Pharmaceutical Research, 14(4), 516-21, April 1997
- Basu S, Wickstrom E, Temperature and salt dependence of higher order structure formation by antisense c-myc and c-myb phosphorothioate oligodeoxyribonucleotides containing tetraguanylate tracts., Nucleic Acids Research, 25(7), 1327-32, 1 Apr 1997
- Mazumder A, Uchida H, Neamati N, Sunder S, Jaworska-Maslanka M, Wickstrom E, Zeng F, Jones RA, Mandes RF, Chenault HK, Pommier Y, Probing interactions between viral DNA and human immunodeficiency virus type 1 integrase using dinucleotides, Molecular Pharmacology, 51(4), 567-75, April 1997
- Cheng X, DeLong R K, Wickstrom E, Kligshteyn M, Demirdji S H, Caruthers M H, Juliano R L, Interactions between single-stranded DNA binding protein and oligonucleotide analogs with different backbone chemistries., Journal of Molecular Recognition, 10(2), 101-7, March 1997
- Wickstrom E, Tyson F L, Differential oligonucleotide activity in cell culture versus mouse models., Ciba Foundation Symposium, 209, 124-37; discussion 1, 1997
- Gray G D, Wickstrom E, Evaluation of anchorage-independent proliferation in tumorigenic cells using the redox dye alamarBlue., Biotechniques, 21(5), 780,782, November 1996
- Fu Z F, Wickstrom E, Jiang M, Corisdeo S, Yang J, Dietzschold B, Koprowski H, Inhibition of rabies virus infection by an oligodeoxynucleotide complementary to rabies virus genomic RNA., Antisense and Nucleic Acid Drug Development, 6(2), 87-93, 1 Jul 1996
- Huang Y, Snyder R, Kligshteyn M, Wickstrom E, Prevention of tumor formation in a mouse model of Burkitt's lymphoma by 6 weeks of treatment with anti-c-myc DNA phosphorothioate., Molecular Medicine, 1(6), 647-58, September 1995
- Hughes J A, Avrutskaya A V, Brouwer K L, Wickstrom E, Juliano R L, Radiolabeling of methylphosphonate and phosphorothioate oligonucleotides and evaluation of their transport in everted rat jejunum sacs., Pharmaceutical Research, 12(6), 817-24, June 1995
- Vyazovkina E V, Savchenko E V, Lokhov S G, Engels J W, Wickstrom E, Lebedev A V, Synthesis of specific diastereomers of a DNA methylphosphonate heptamer, d(CpCpApApApCpA), and stability of base pairing with the normal DNA octamer d(TPGPTPTPTPGPGPC)., Nucleic Acids Research, 22(12), 2404-9, 25 Jun 1994
- Vyazovkina E V, Rife J P, Lebedev A V, Wickstrom E, Preparation of trimers and tetramers of mixed sequence oligodeoxynucleoside methylphosphonates and assignment of configurations at the chiral phosphorus., Nucleic Acids Research, 21(25), 5957-63, 25 Dec 1993
- Gray G D, Hernandez O M, Hebel D, Root M, Pow-Sang J M, Wickstrom E, Antisense DNA inhibition of tumor growth induced by c-Ha-ras oncogene in nude mice., Cancer Research, 53(3), 577-80, 1 Feb 1993
- Wickstrom E, Bacon T A, Wickstrom E L, Down-regulation of c-MYC antigen expression in lymphocytes of Emu-c-myc transgenic mice treated with anti-c-myc DNA methylphosphonates., Cancer Research, 52(24), 6741-5, 15 Dec 1992
- Wickstrom E, Strategies for administering targeted therapeutic oligodeoxynucleotides., Trends in Biotechnology, 10(8), 281-7, August 1992
- Akhtar S, Basu S, Wickstrom E, Juliano R L, Interactions of antisense DNA oligonucleotide analogs with phospholipid membranes (liposomes)., Nucleic Acids Research, 19(20), 5551-9, 25 Oct 1991
- Farese RV, Standaert ML, Ishizuka T, Yu B, Hernandez H, Waldron C, Watson J, Farese JP, Cooper DR, Wickstrom E, Antisense DNA downregulates protein kinase C isozymes (beta and alpha) and insulin-stimulated 2-deoxyglucose uptake in rat adipocytes, Antisense Research and Development, 1(1), 35-42, Spring 1991
- Wickstrom E, Bacon T A, Wickstrom E L, Werking C M, Palmiter R D, Brinster R L, Sandgren E P, Antisense oligodeoxynucleoside methylphosphonate inhibition of mouse c-myc p65 protein expression in E mu-c-myc transgenic mice., Nucleic Acids Symposium Series, 1991
- Bacon T A, Wickstrom E, Daily addition of an anti-c-myc DNA oligomer induces granulocytic differentiation of human promyelocytic leukemia HL-60 cells in both serum-containing and serum-free media., Oncogene Research, 6(1), 21-32, 1991
- Bacon T A, Wickstrom E, Walking along human c-myc mRNA with antisense oligodeoxynucleotides: maximum efficacy at the 5' cap region., Oncogene Research, 6(1), 13-9, 1991
- Ho PT, Ishiguro K, Wickstrom E, Sartorelli AC, Non-sequence-specific inhibition of transferrin receptor expression in HL-60 leukemia cells by phosphorothioate oligodeoxynucleotides, Antisense Research and Development, 1(4), 329-42, Winter 1991
- Campbell J M, Bacon T A, Wickstrom E, Oligodeoxynucleoside phosphorothioate stability in subcellular extracts, culture media, sera and cerebrospinal fluid., Journal of Biochemical and Biophysical Methods, 20(3), 259-67, March 1990
- Daaka Y, Wickstrom E, Target dependence of antisense oligodeoxynucleotide inhibition of c-Ha-ras p21 expression and focus formation in T24-transformed NIH3T3 cells., Oncogene Research, 5(4), 267-75, 1990
- Muralikrishna P, Wickstrom E, Escherichia coli initiation factor 3 protein binding to 30S ribosomal
subunits alters the accessibility of nucleotides within the conserved
central region of 16S rRNA, Biochemistry, 28(19), 7505-10, Sep 1989
- Muralikrishna P, Wickstrom E, Inducible high expression of the Escherichia coli infC gene subcloned behind a bacteriophage T7 promoter, Gene, 80(2), 369-74, August 1989
- Wickstrom E L, Bacon T A, Gonzalez A, Lyman G H, Wickstrom E, Anti-c-myc DNA increases differentiation and decreases colony formation by HL-60 cells., In Vitro Cellular and Developmental Biology, 25(3 Pt 1), 297-302, March 1989
- Lise LD, Jolivet M, Audibert F, Fernandez A, Wickstrom E, Chedid L, Schlesinger DH, Role of the proline residues on the immunogenic properties of a P. falciparum circumsporozoite peptide linked to a carrier protein, Peptide Research, 2(1), 114-9, 1989
- Bacon TA, Morvan F, Rayner B, Imbach JL, Wickstrom E, alpha-Oligodeoxynucleotide stability in serum, subcellular extracts and culture media, Journal of Biochemical and Biophysical Methods, 16(4), 311-8, August 1988
- Wickstrom EL, Bacon TA, Gonzalez A, Freeman DL, Lyman GH, Wickstrom E, Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA, Proceedings of the National Academy of Sciences (USA), 85(4), 1028-32, February 1988
- Wickstrom E, Laing LG, Physical studies of the interaction of Escherichia coli translational initiation factor 3 protein with ribosomal RNA, Methods in Enzymology, 164, 238-58, 1988
- Heikkila R, Schwab G, Wickstrom E, Loke SL, Pluznik DH, Watt R, Neckers LM, A c-myc antisense oligodeoxynucleotide inhibits entry into S phase but not progress from G0 to G1, Nature, 328(6129), 445-9, July 1987
- Wickstrom E, Escherichia coli ribosomal protein S1 does not protect the 49-nucleotide 3' terminal cloacin fragment of 16 S rRNA from nuclease S1, Biochimica Et Biophysica Acta, 868(4), 265-9, December 1986
- Wickstrom E, Oligodeoxynucleotide stability in subcellular extracts and culture media, Journal of Biochemical and Biophysical Methods, 13(2), 97-102, Sep 1986
- Wickstrom E, Heus HA, Haasnoot CA, van Knippenberg PH, Circular dichroism and 500-MHz proton magnetic resonance studies of the interaction of Escherichia coli translational initiation factor 3 protein with the 16S ribosomal RNA 3' cloacin fragment, Biochemistry, 25(10), 2770-7, May 1986
- Wickstrom E, Nuclease mapping of the secondary structure of the 49-nucleotide 3' terminal cloacin fragment of Escherichia coli 16s RNA and its interactions with initiation factor 3, Nucleic Acids Research, 11(7), 2035-52, April 1983
- Parker KK, Wickstrom E, Crosslinking of Escherichia coli 50S ribosomal subunits with chlorambucilyl oligoprolyl phenylalanyl-tRNA molecular rulers, Nucleic Acids Research, 11(2), 515-24, January 1983
- Skidgel RA, Wickstrom E, Kumamoto K, Erdos EG, Rapid radioassay for prolylcarboxypeptidase (angiotensinase C), Analytical Biochemistry, 118(1), 113-9, November 1981
- Yager TD, Reuben MA, Ainpour PR, Wickstrom E, Molecular rulers with 5 or 11 prolines intramolecularly crosslink to G45 of yeast phenylalanine tRNA, Febs Letters, 133(1), 59-62, October 1981
- Wickstrom E, Physical parameters of Escherichia coli translational initiation factor 3 binding to poly(A), Febs Letters, 128(1), 154-6, June 1981
- Reuben MA, Ainpour PR, Hester HL, Neveln VL, Wickstrom E, Synthesis and intramolecular crosslinking of chlorambucilyl (prolyl)n [3H]phenylalanyl-tRNAPhe (yeast), n = 0, 5, 11 and 15, Biochimica Et Biophysica Acta, 654(1), 11-25, June 1981
- Wickstrom E, Behlen LS, Reuben MA, Ainpour PR, Molecular rulers for measuring RNA structure: sites of crosslinking in chlorambucilyl-phenylalanyl-tRNAPhe (yeast) and chlorambucilyl-pentadecaprolyl-phenylalanyl-tRNAPhe (yeast) intramolecularly cros, Proceedings of the National Academy of Sciences (USA), 78(4), 2082-5, April 1981
- Wickstrom E, Parker KK, Hursh DA, Newton RL, Chlorambucil [3H]phenylalanyl-tRNA crosslinking to 50 S ribosomal subunit proteins L4, L26--27 and L18--20, Febs Letters, 123(2), 273-6, January 1981
- Wickstrom E, Chlorambucil inhibition by dimethyl sulfoxide and thiosulfate: implications for chlorambucil chemotherapy, Medical Hypotheses, 6(10), 1035-41, October 1980
- Schleich T, Wickstrom E, Twombly K, Schmidt B, Tyson RW, Circular dichroism study of Escherichia coli initiation factor 3 binding to nucleic acids, Biochemistry, 19(19), 4486-92, September 1980
- Wickstrom E, Tyson RW, Newton G, Obert R, Williams EE, Stoichiometry of homopolynucleotide binding to Escherichia coli translational initiation factor 3, Archives of Biochemistry and Biophysics, 200(1), 296-300, March 1980
- Ainpour PR, Wickstrom E, Modifications of solid phase peptide synthesis to obtain homogeneous oligoprolines in high yield, International Journal of Peptide and Protein Research, 15(3), 225-35, March 1980
- Reuben MA, Kusnezov IJ, Wickstrom E, Inhibition of deacylation and improvement in N-hydroxysuccinimide ester modification of phenylalanyl-tRNA by dimethyl sulfoxide, Biochimica Et Biophysica Acta, 565(1), 219-23, November 1979
- Wickstrom E, Tinoco I Jr, The stability of RNA hairpin loops containing A-U-G: An-U-G-Um, Biopolymers, 13(11), 2367-83, November 1974
- Wickstrom E, Escherichia coli initiation factor IF3 binding to AUG and AUG-containing
single strands and hairpin loops, and nonspecific binding to
polymers, Biochimica Et Biophysica Acta, 349(1), 125-30, Apr 1974
- Wickstrom E, Circular dichroism during deacylation of methionyl-tRNA met -f and formylmethionyl-tRNA met -f from E. coli, Biochemical and Biophysical Research Communications, 43(5), 976-83, June 1971
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